The Effect of Chemical Treatments of Albumin and Orosomucoid on
... protein in its monomeric form, and those of another may relate to it in some ill-defined aggregated form. Meaningful comparison of such results is impossible, since two such preparations of the same protein may each be taken up preferentially by a different cell type. Such studies are often further ...
... protein in its monomeric form, and those of another may relate to it in some ill-defined aggregated form. Meaningful comparison of such results is impossible, since two such preparations of the same protein may each be taken up preferentially by a different cell type. Such studies are often further ...
medline:93194904
... TNF-alpha were released into the culture media. Although okadaic acid provides a ...
... TNF-alpha were released into the culture media. Although okadaic acid provides a ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessary for these pumpkin storage proteins to pass through the G ...
... are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessary for these pumpkin storage proteins to pass through the G ...
Selective Dimerization of a C2H2 Zinc Finger Subfamily
... et al., 2000). In addition to the cysteines and histidines that coordinate zinc, C2H2 fingers contain conserved hybrophobic residues that pack in the hydrophobic core. These conserved amino acids lead to the formation of the characteristic structure comprised of a twostranded antiparallel  sheet an ...
... et al., 2000). In addition to the cysteines and histidines that coordinate zinc, C2H2 fingers contain conserved hybrophobic residues that pack in the hydrophobic core. These conserved amino acids lead to the formation of the characteristic structure comprised of a twostranded antiparallel  sheet an ...
The role of mTOR signaling in the regulation of protein synthesis
... (Goodman et al., 2011c). For instance, a net decrease in protein synthesis and/or a net increase in protein degradation can lead to disuse atrophy. Indeed, both decreased rates of protein synthesis and increased rates of protein degradation have been observed in several animal models of disuse atrop ...
... (Goodman et al., 2011c). For instance, a net decrease in protein synthesis and/or a net increase in protein degradation can lead to disuse atrophy. Indeed, both decreased rates of protein synthesis and increased rates of protein degradation have been observed in several animal models of disuse atrop ...
lncRNA in
... • Look for structural evidence – Structural possibility does not mean non-coding • Look for sequence binding motif – Could still be protein coding • Look for sequence similarity to known lncRNA – Not enough known about lncRNA and low evolutionary conservation ...
... • Look for structural evidence – Structural possibility does not mean non-coding • Look for sequence binding motif – Could still be protein coding • Look for sequence similarity to known lncRNA – Not enough known about lncRNA and low evolutionary conservation ...
Designed sequences improve distant homology detection
... the test. OB-fold adopts a beta-barrel structure consisting of 5 beta strands. OB-fold is characteristic of a wide variety of proteins, most of which are involved in oligonucleotide or oligosaccharide binding. In the SCOP (Structural Classification of Proteins, version 1.57) (22) database, OB-fold i ...
... the test. OB-fold adopts a beta-barrel structure consisting of 5 beta strands. OB-fold is characteristic of a wide variety of proteins, most of which are involved in oligonucleotide or oligosaccharide binding. In the SCOP (Structural Classification of Proteins, version 1.57) (22) database, OB-fold i ...
Proteomic Approach to Identify Novel
... proteins are freshly generated by the mitochondrial processing peptidase shortly after import of proteins into the organelle. The determination of peptide masses of trypsinated spots by matrix-assisted laserdesorption ionization-mass spectrometry also led to successful identification of proteins, be ...
... proteins are freshly generated by the mitochondrial processing peptidase shortly after import of proteins into the organelle. The determination of peptide masses of trypsinated spots by matrix-assisted laserdesorption ionization-mass spectrometry also led to successful identification of proteins, be ...
Protein Nutrition of Dairy Cattle – An Overview
... requirement for the limiting amino acid is met. Within a group of animals or birds given ...
... requirement for the limiting amino acid is met. Within a group of animals or birds given ...
Protein splicing of PRP8 mini-inteins from species of the genus
... The prp8 gene of three Penicillium species contains an intein To assess the occurrence of inteins in the prp8 gene of species of the genus Penicillium, putative intein-containing fragments, according to the insertion site of previously identified C. neoformans, A. nidulans, A. fumigatus, and H. caps ...
... The prp8 gene of three Penicillium species contains an intein To assess the occurrence of inteins in the prp8 gene of species of the genus Penicillium, putative intein-containing fragments, according to the insertion site of previously identified C. neoformans, A. nidulans, A. fumigatus, and H. caps ...
Channel Protein From Rat Heart Using Subtype
... of the primary sequence that demonstrate high homology among subtypes, it is not clear whether the unique characteristics of each subtype are due to differences in channel primary sequence, posttranslational modification, presence of associated f, and/or /32 subunits, or a combination of the above f ...
... of the primary sequence that demonstrate high homology among subtypes, it is not clear whether the unique characteristics of each subtype are due to differences in channel primary sequence, posttranslational modification, presence of associated f, and/or /32 subunits, or a combination of the above f ...
Charge asymmetry in the proteins of the outer membrane
... found in the outer membrane of bacteria, mitochondria and chloroplasts. There are thousands of beta-barrels reported in genomic databases with 2–3% of the genes in gram-negative bacteria encoding these proteins. These proteins have a wide variety of biological functions including active and passive ...
... found in the outer membrane of bacteria, mitochondria and chloroplasts. There are thousands of beta-barrels reported in genomic databases with 2–3% of the genes in gram-negative bacteria encoding these proteins. These proteins have a wide variety of biological functions including active and passive ...
Protein Applications in Sports Nutrition—Part I
... IMPORTANT TO MAXIMALLY STIMULATE MUSCLE PROTEIN SYNTHESIS AND PROMOTE A POSITIVE MUSCLE PROTEIN BALANCE. OVERVIEW ...
... IMPORTANT TO MAXIMALLY STIMULATE MUSCLE PROTEIN SYNTHESIS AND PROMOTE A POSITIVE MUSCLE PROTEIN BALANCE. OVERVIEW ...
Antioxidant and antimicrobial activity of lecithin free egg yolk protein
... studies reported the antioxidant activity of egg-yolk protein in a linoleic acid oxidation system [3,4]. The hydroxyl radical and DPPH scavenging activity and suppression of discoloration of βcarotene have been also observed. In food modeling systems, peptides derived from egg-yolk hydrolysates effe ...
... studies reported the antioxidant activity of egg-yolk protein in a linoleic acid oxidation system [3,4]. The hydroxyl radical and DPPH scavenging activity and suppression of discoloration of βcarotene have been also observed. In food modeling systems, peptides derived from egg-yolk hydrolysates effe ...
NF-kB as a primary regulator of the stress response
... recent report demonstrated via phosphopeptide mapping studies that IKK2 is phosphorylated at Ser 177 and Ser 181 in response to pro-in¯amatory cytokines (Delhase et al., 1999). These studies strongly suggest that the IKKs are themselves phosphorylated and activated by one or more upstream kinases. M ...
... recent report demonstrated via phosphopeptide mapping studies that IKK2 is phosphorylated at Ser 177 and Ser 181 in response to pro-in¯amatory cytokines (Delhase et al., 1999). These studies strongly suggest that the IKKs are themselves phosphorylated and activated by one or more upstream kinases. M ...
JNK Regulates MCP-1 Expression in Adenovirus Type 19
... arthritis, and bacterial meningitis (for a review, see Luster9). It has been shown that inhibiting MCP-1 expression results in reduced transmigration of monocytes through blood vessels10 and in diminished recruitment of T lymphocytes,8 suggesting a critical role in acute inflammation. Human keratocy ...
... arthritis, and bacterial meningitis (for a review, see Luster9). It has been shown that inhibiting MCP-1 expression results in reduced transmigration of monocytes through blood vessels10 and in diminished recruitment of T lymphocytes,8 suggesting a critical role in acute inflammation. Human keratocy ...
Simultaneous Alignment and Folding of Protein Sequences
... structure. Moreover, for proteins, there is no clear chemical basis for compensatory mutations [11], the energy models that define β-strand pairings are more complex, and the larger residue alphabet vastly increases the complexity of the problem. This class of problems is also different than any tha ...
... structure. Moreover, for proteins, there is no clear chemical basis for compensatory mutations [11], the energy models that define β-strand pairings are more complex, and the larger residue alphabet vastly increases the complexity of the problem. This class of problems is also different than any tha ...
Ribosomes and Protein Synthesis
... interaction of tRNA and the template RNA, considered to exist on the ribosome as a mechanism for ordering the amino acid sequence (62). Although Crick suggested the presence of a trinucleotide as a translation piece, the tRNA actually found contained approximately 75 nucleotides . It was determined ...
... interaction of tRNA and the template RNA, considered to exist on the ribosome as a mechanism for ordering the amino acid sequence (62). Although Crick suggested the presence of a trinucleotide as a translation piece, the tRNA actually found contained approximately 75 nucleotides . It was determined ...
Glycophorin C Content of Human Erythrocyte
... except 5% (wt/vol) low-fat milk in phosphate-buffered saline at pH 7.4 was used as the blocking reagent. ...
... except 5% (wt/vol) low-fat milk in phosphate-buffered saline at pH 7.4 was used as the blocking reagent. ...
Tubulin folding is altered by mutations in a putative GTP binding motif
... Weisenberg et al., 1976). β-tubulin is a GTPase, while αtubulin has no enzyme activity (Carlier, 1982). Tubulins have an invariant region rich in glycines that is found in α-, β-, and γ-chains and which is presumed to form a phosphate-binding loop (Burns, 1995). The consensus phosphate binding site ...
... Weisenberg et al., 1976). β-tubulin is a GTPase, while αtubulin has no enzyme activity (Carlier, 1982). Tubulins have an invariant region rich in glycines that is found in α-, β-, and γ-chains and which is presumed to form a phosphate-binding loop (Burns, 1995). The consensus phosphate binding site ...
Supporting Information
... Fig. S1. Comparison of the RPE65 and ACO crystal structures. (A) Structural superposition of RPE65 (blue; PDB ID code 3FSN) and ACO (green; PDB ID code 2BIW). The two structures superimpose with an rmsd of 2.5 Å over 443 matched C␣ positions. The most notable differences are found in the helical cap ...
... Fig. S1. Comparison of the RPE65 and ACO crystal structures. (A) Structural superposition of RPE65 (blue; PDB ID code 3FSN) and ACO (green; PDB ID code 2BIW). The two structures superimpose with an rmsd of 2.5 Å over 443 matched C␣ positions. The most notable differences are found in the helical cap ...
Proteomic Analysis of the Arabidopsis Nucleolus Suggests Novel
... Lamond, 1999; Phair and Misteli, 2000; Chen and Huang, 2001; Leung and Lamond, 2002; Lamond and Spector, 2003). Although the fundamental processes of ribosome biogenesis are very similar in all eukaryotes, there are significant ...
... Lamond, 1999; Phair and Misteli, 2000; Chen and Huang, 2001; Leung and Lamond, 2002; Lamond and Spector, 2003). Although the fundamental processes of ribosome biogenesis are very similar in all eukaryotes, there are significant ...
University of Groningen Plasticity of airway smooth muscle
... in part be responsible for this process by increasing cell number (hyperplasia) or size (hypertrophy) through the activation of receptor tyrosine kinases. In addition, G protein coupled receptor (GPCR) agonists may contribute to this increase in ASM ...
... in part be responsible for this process by increasing cell number (hyperplasia) or size (hypertrophy) through the activation of receptor tyrosine kinases. In addition, G protein coupled receptor (GPCR) agonists may contribute to this increase in ASM ...
Tangping Li, Ke Fan, Jun Wang and Wei Wang Reduction of protein
... substitution matrix is necessary, in which different scores are assigned to different exchanges of one amino acid with another. In general, a positive score indicates that two residues are similar, and substitution or mutation between them may be applicable. While a negative score implies that two r ...
... substitution matrix is necessary, in which different scores are assigned to different exchanges of one amino acid with another. In general, a positive score indicates that two residues are similar, and substitution or mutation between them may be applicable. While a negative score implies that two r ...
Chemical synthesis of proteins
... for specific functions. There are three general chemical approaches to constructing proteins. The first is ‘stepwise synthesis’, in which the entire protein is synthesized one amino acid at a time. The second is ‘fragment assembly’, in which individual peptide strands are initially constructed stepw ...
... for specific functions. There are three general chemical approaches to constructing proteins. The first is ‘stepwise synthesis’, in which the entire protein is synthesized one amino acid at a time. The second is ‘fragment assembly’, in which individual peptide strands are initially constructed stepw ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).