Strong and weak hydrogen bonds in the protein
... and acceptors agree with Lipinski’s rule-of-five that predicts drug-like properties. Hydrogen bonds formed by water were also seen to be relevant in ligand binding and ligand C HOw interactions are abundant when compared to N HOw and O HOw. Among the limitations of our earlier study w ...
... and acceptors agree with Lipinski’s rule-of-five that predicts drug-like properties. Hydrogen bonds formed by water were also seen to be relevant in ligand binding and ligand C HOw interactions are abundant when compared to N HOw and O HOw. Among the limitations of our earlier study w ...
9 The AMP-activated protein kinase: more than an energy sensor
... regulatory ( and ␥) subunits. Each subunit has multiple isoforms (␣1, ␣2, 1, 2, ␥1, ␥2, ␥3) giving twelve possible combinations of holoenzyme with different tissue distributions. In skeletal muscle, only the ␣12␥1, ␣22␥1 and ␣22␥3 complexes seem to be expressed [3]. The N-terminus of the ␣subu ...
... regulatory ( and ␥) subunits. Each subunit has multiple isoforms (␣1, ␣2, 1, 2, ␥1, ␥2, ␥3) giving twelve possible combinations of holoenzyme with different tissue distributions. In skeletal muscle, only the ␣12␥1, ␣22␥1 and ␣22␥3 complexes seem to be expressed [3]. The N-terminus of the ␣subu ...
+Tec - Blood Journal
... cells. This peptide could not be detected in the lysate of 3T3 cells that do not express the tec message endogenously (data not shown). Therefore, we conclude that Tec type IV is a peptide of 70 kD in vivo. A peptide of approximately 80 kD observed in Fig 3, lane 32D, is of the nonspecific binding o ...
... cells. This peptide could not be detected in the lysate of 3T3 cells that do not express the tec message endogenously (data not shown). Therefore, we conclude that Tec type IV is a peptide of 70 kD in vivo. A peptide of approximately 80 kD observed in Fig 3, lane 32D, is of the nonspecific binding o ...
Statistical Analysis of Amino Acid Patterns in Transmembrane
... neighboring b-branched residues Ile and Val. In fact, the GxxxG pair has been described as part of the strong interaction motif in the glycophorin A transmembrane dimer, in which the pair is associated with two Val residues (GVxxGV). GxxxG is also the major motif identi®ed using TOXCAT, an in vivo s ...
... neighboring b-branched residues Ile and Val. In fact, the GxxxG pair has been described as part of the strong interaction motif in the glycophorin A transmembrane dimer, in which the pair is associated with two Val residues (GVxxGV). GxxxG is also the major motif identi®ed using TOXCAT, an in vivo s ...
Evolution of hard proteins in the sauropsid integument in relation to
... layer of flat cells that are incorporated into a variably thick corneous layer (depending on the type of scales) (Fig. 1I). In archosaurians (crocodilians and birds), the process of shedding of epidermal layers is not specifically known. However, the presence of a scission layer in avian scales (Can ...
... layer of flat cells that are incorporated into a variably thick corneous layer (depending on the type of scales) (Fig. 1I). In archosaurians (crocodilians and birds), the process of shedding of epidermal layers is not specifically known. However, the presence of a scission layer in avian scales (Can ...
The Proteomic Code: a molecular recognition code for proteins
... found that these specially coded amino acid pairs are statistically overrepresented in those proteins known to interact with each other. In addition, I was able to find short complementary amino acid sequences within the same protein sequences and inferred that these might play a role in the formati ...
... found that these specially coded amino acid pairs are statistically overrepresented in those proteins known to interact with each other. In addition, I was able to find short complementary amino acid sequences within the same protein sequences and inferred that these might play a role in the formati ...
Holding it all together? Candidate proteins for the plant Golgi matrix
... minus of the protein encoded by ORF At3 g18480 shows homology to the TM domain of human CASP (58% identity) [24,43]. The similarity between these two proteins is, however, also evident in other regions of the protein. The membrane topologies of At1 g18190, At2 g19950 and At3 g18480 resemble those of ...
... minus of the protein encoded by ORF At3 g18480 shows homology to the TM domain of human CASP (58% identity) [24,43]. The similarity between these two proteins is, however, also evident in other regions of the protein. The membrane topologies of At1 g18190, At2 g19950 and At3 g18480 resemble those of ...
Document
... one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell. 2002 Sep;10(3):457-68. Ashe MP, De Long SK, Sachs AB. Glucose depletion rapidly inhibits translation initiation in yeast. Mol Biol Cell. 2000 Mar;11(3):83348. Crespo JL, Powers T, Fowler B, Hall MN. The TOR-co ...
... one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell. 2002 Sep;10(3):457-68. Ashe MP, De Long SK, Sachs AB. Glucose depletion rapidly inhibits translation initiation in yeast. Mol Biol Cell. 2000 Mar;11(3):83348. Crespo JL, Powers T, Fowler B, Hall MN. The TOR-co ...
Full version (PDF file)
... only 0.001 % of total glucose concentration, α-oxoaldehydes are characterized by extremely high chemical activity. They are up to 20,000-fold as reactive as glucose, thus being able to form AGE-structures even at very low concentrations. Physiologically, α-oxoaldehydes react directly with protein te ...
... only 0.001 % of total glucose concentration, α-oxoaldehydes are characterized by extremely high chemical activity. They are up to 20,000-fold as reactive as glucose, thus being able to form AGE-structures even at very low concentrations. Physiologically, α-oxoaldehydes react directly with protein te ...
You can answer the question Yourself with a few
... including it in your daily regimen. It’s enormously advantageous for several reasons, including amino acid and microfraction content (more on that in a minute) and digestion rate. Whey is one of the richest sources of ...
... including it in your daily regimen. It’s enormously advantageous for several reasons, including amino acid and microfraction content (more on that in a minute) and digestion rate. Whey is one of the richest sources of ...
Isolation and Expression Pattern of Human Unc-33
... washed three times in PBS, then fixed for 1 hr in 4% paraformaldehyde. After one wash in PBS, endogenous phosphatases were heat-inactivated at 65°C for 50 min, then the preparations were equilibrated for 20 min with AP buffer (100 mM Tris, 100 mM NaC l, and 5 mM MgC l2, pH 9.5), and the bound AP-Sem ...
... washed three times in PBS, then fixed for 1 hr in 4% paraformaldehyde. After one wash in PBS, endogenous phosphatases were heat-inactivated at 65°C for 50 min, then the preparations were equilibrated for 20 min with AP buffer (100 mM Tris, 100 mM NaC l, and 5 mM MgC l2, pH 9.5), and the bound AP-Sem ...
Role of lectins (and rhizobial exopolysaccharides) in legume
... formulate the lectin recognition hypothesis have no transmembrane domains that could connect directly to components of a signal transduction cascade. Legume seed lectins are usually hololectins with two or more carbohydrate-binding sites, one per subunit; the carbohydrate binding domains are identic ...
... formulate the lectin recognition hypothesis have no transmembrane domains that could connect directly to components of a signal transduction cascade. Legume seed lectins are usually hololectins with two or more carbohydrate-binding sites, one per subunit; the carbohydrate binding domains are identic ...
soy protein concentrate - US Soybean Export Council
... Information in this technical bulletin was provided by The Solae Company, St. Louis, Missouri, USA. The U.S. Soybean Export Council sincerely thanks The Solae Company for its cooperation and assistance in producing this technical bulletin. For more information about The Solae Company, please see the ...
... Information in this technical bulletin was provided by The Solae Company, St. Louis, Missouri, USA. The U.S. Soybean Export Council sincerely thanks The Solae Company for its cooperation and assistance in producing this technical bulletin. For more information about The Solae Company, please see the ...
Current Opinion in Plant Biology
... formulate the lectin recognition hypothesis have no transmembrane domains that could connect directly to components of a signal transduction cascade. Legume seed lectins are usually hololectins with two or more carbohydrate-binding sites, one per subunit; the carbohydrate binding domains are identic ...
... formulate the lectin recognition hypothesis have no transmembrane domains that could connect directly to components of a signal transduction cascade. Legume seed lectins are usually hololectins with two or more carbohydrate-binding sites, one per subunit; the carbohydrate binding domains are identic ...
Insulin action on skeletal muscle protein metabolism during
... that their effect was artefactual. For example, the strong stimulation of muscle protein synthesis recently observed in the forearm [42] could be related to an IGF11 receptor signalling owing to the extreme hyperinsulinemia. The study by Bennett et al. [8] compared the effect of amino acid infusion ...
... that their effect was artefactual. For example, the strong stimulation of muscle protein synthesis recently observed in the forearm [42] could be related to an IGF11 receptor signalling owing to the extreme hyperinsulinemia. The study by Bennett et al. [8] compared the effect of amino acid infusion ...
NSPKU
... This blood test is carried out by the midwife around 5 days after birth. Under the national newborn screening programme every baby in the UK and Ireland is screened for this condition. PKU is then picked up before any harm is caused. ...
... This blood test is carried out by the midwife around 5 days after birth. Under the national newborn screening programme every baby in the UK and Ireland is screened for this condition. PKU is then picked up before any harm is caused. ...
Sixth Southeast Enzyme Conference
... The Chemistry Department at Georgia State University is the lead organizer and founder of the Southeast Enzyme Conference. This conference attracts students, research scientists and faculty members from colleges and universities throughout the southeastern region. With the assistance of the GSU Foun ...
... The Chemistry Department at Georgia State University is the lead organizer and founder of the Southeast Enzyme Conference. This conference attracts students, research scientists and faculty members from colleges and universities throughout the southeastern region. With the assistance of the GSU Foun ...
Materials - HAL
... and Psp5II restriction sites were introduced in SacII-NotI sites of pHM6 vector. The cDNA encoding the N-terminal part of PTPL1 was cloned by PCR using pSV7d-PTPL1 (gift of Prof. C.H. Heldin) as a template in SacII-Bsu15I sites of modified pHM6 vector. SacII site was introduced by PCR in frame with ...
... and Psp5II restriction sites were introduced in SacII-NotI sites of pHM6 vector. The cDNA encoding the N-terminal part of PTPL1 was cloned by PCR using pSV7d-PTPL1 (gift of Prof. C.H. Heldin) as a template in SacII-Bsu15I sites of modified pHM6 vector. SacII site was introduced by PCR in frame with ...
Bile Acids and Bile Acid Sequestrants
... family of intracellular transcription factors and is encoded by the NR0B2 (nuclear receptor subfamily 0, group B, member 2) gene. SHP is unusual for a nuclear receptor in that it lacks a DNA binding domain. Therefore technically it is neither a transcription factor nor nuclear receptor but neverthel ...
... family of intracellular transcription factors and is encoded by the NR0B2 (nuclear receptor subfamily 0, group B, member 2) gene. SHP is unusual for a nuclear receptor in that it lacks a DNA binding domain. Therefore technically it is neither a transcription factor nor nuclear receptor but neverthel ...
PDF
... Robishaw, 1984; Wolfgang et al., 1990). It is the immediate transducer of a number of G protein-coupled receptors (GPCRs), including receptors of the Frizzled (Fz) family (Egger-Adam and Katanaev, 2008). In Drosophila, Go is involved in transduction of the Wingless (Wg; Drosophila Wnt1) signal (Kata ...
... Robishaw, 1984; Wolfgang et al., 1990). It is the immediate transducer of a number of G protein-coupled receptors (GPCRs), including receptors of the Frizzled (Fz) family (Egger-Adam and Katanaev, 2008). In Drosophila, Go is involved in transduction of the Wingless (Wg; Drosophila Wnt1) signal (Kata ...
YangSpr07
... or RGD present on extracellular matrix proteins is known to be a requirement for binding to cell surface receptor proteins, the integrins. The binding of extracellular matrix proteins to the integrin receptors by the RGD sequence involves a number of important cellular processes, such as cell anchor ...
... or RGD present on extracellular matrix proteins is known to be a requirement for binding to cell surface receptor proteins, the integrins. The binding of extracellular matrix proteins to the integrin receptors by the RGD sequence involves a number of important cellular processes, such as cell anchor ...
Statistical potential-based amino acid similarity
... sequence, which do not necessarily have an evolutionary relationship, have a similar local structure.6 – 8 Of course, these two approaches are not necessarily mutually exclusive, and they are often combined as terms in the scoring function of “ab initio” or “de novo” protein structure prediction alg ...
... sequence, which do not necessarily have an evolutionary relationship, have a similar local structure.6 – 8 Of course, these two approaches are not necessarily mutually exclusive, and they are often combined as terms in the scoring function of “ab initio” or “de novo” protein structure prediction alg ...
Feeney_ku_0099D_12934_DATA_1 - KU ScholarWorks
... Purpose: Oxidative post-translational modification of protein-bound tyrosine residues can have a significant impact on protein structure and function and thus may be important to physiological and pathological processes. Oxidative stress has been correlated with biological aging and many disease sta ...
... Purpose: Oxidative post-translational modification of protein-bound tyrosine residues can have a significant impact on protein structure and function and thus may be important to physiological and pathological processes. Oxidative stress has been correlated with biological aging and many disease sta ...
Multivariate Modeling of Proteins Related to
... validation (SIMCA-P version 12, Umetrics AB, Umeå, Sweden). This method keeps part of the data out from the model development to assess the predictive power of the model and was used to test the significance of the components. Hence, this validation technique increases the stability of the results. ...
... validation (SIMCA-P version 12, Umetrics AB, Umeå, Sweden). This method keeps part of the data out from the model development to assess the predictive power of the model and was used to test the significance of the components. Hence, this validation technique increases the stability of the results. ...
Mesoderm and Endoderm Formation
... Conclusions from Nieuwkoop’s Experiments 1. Mesoderm is induced when endoderm and endoderm are juxtaposed. 2. Signals arise from endoderm and received by ectoderm. 3. The signal from the endoderm is secreted. 4. Mesoderm induction is independent of protein synthesis. 5. The signal can only act over ...
... Conclusions from Nieuwkoop’s Experiments 1. Mesoderm is induced when endoderm and endoderm are juxtaposed. 2. Signals arise from endoderm and received by ectoderm. 3. The signal from the endoderm is secreted. 4. Mesoderm induction is independent of protein synthesis. 5. The signal can only act over ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).