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RNA Structure, Function, and Synthesis RNA - Rose
RNA Structure, Function, and Synthesis RNA - Rose

... Template strand: the DNA sequence that the polymerase actually uses to guide the synthesis of the growing RNA strand. The template strand is complementary to both the RNA strand and the DNA coding strand. Upstream: on the 5´side of any given position on the coding strand. Downstream: on the 3´side o ...
PPT
PPT

... Observed pattern – All phylograms yielded geographic-based clustering rather than timebased clustering – Host ranges along clustered clades vary – Same strain with identical NA sequences can infect different hosts – NA may not be the sole factor responsible for determining the diverse host range – G ...
Mitochondria tutorial
Mitochondria tutorial

... three buttons labeled create map, clear DNA, and get demo DNA. Now, paste the sequence that you retrieved into the white box. Don't worry about changing the spaces and returns; the program deals with them just fine. Select the Create Map button. Beneath the white box, on the left-hand side, is a sma ...
Gene duplication and evolutionary novelty in
Gene duplication and evolutionary novelty in

... release, could initiate the process of shuffling as well. In addition, this increased transposon activity has been found to alter the expression levels of nearby genes (Kashkush et al., 2003), which may further induce phenotypic alteration following polyploidy (Chen & Ni, 2006). Although there are f ...
−Table of Contents
−Table of Contents

... An identification of the unknown band 1 area was attempted using the uv-vis spectrum obtained. The unknown spectrum did not match up well with that of chalcone because it contained a peak around 270nm that chalcone lacks. The peaks of the unknown at 270nm and 362nm suggest that the unknown is a flav ...
The Aspergillus Genome Database, a curated comparative
The Aspergillus Genome Database, a curated comparative

... a particular species—such as pathogenicity in an animal host, toxin generation or exceptional production of citric acid under industrial conditions—consistency of annotation is essential to ensure that differences in the gene complement reflect actual biological differences, rather than variations amon ...
Figure 2 - GEP Community Server
Figure 2 - GEP Community Server

... using a chemical method to tag the special structure that occurs at 5’ ends of transcript, fishing out the RNA molecules using these tags, and mapping the sequence back to the genome, a method called “CAGE” (cap analysis of gene expression). In addition, we will also display the "D. mel. cDNA" track ...
module 3: transcription part ii
module 3: transcription part ii

... using a chemical method to tag the special structure that occurs at 5’ ends of transcript, fishing out the RNA molecules using these tags, and mapping the sequence back to the genome, a method called “CAGE” (cap analysis of gene expression). In addition, we will also display the "D. mel. cDNA" track ...
Clustering2_11-8
Clustering2_11-8

... Is it possible that some of these gene expression changes are miscalled (i.e. biologically significant but insignificant p value and vice versa) and why? What other criteria might you use to distinguish genes you care about? How many genes pass the cutoff of q<0.01 and how does this compare to the n ...
Lovering presentation
Lovering presentation

... Gene name: a brief and specific description which conveys the character or function of the gene/gene product, but does not attempt to describe everything known about it. Gene Symbol: an abbreviation/acronym of the gene name, designated by upper-case Latin letters or by a combination of upper-case le ...
GENETIC BASICS OF VARIATIONS IN BACTERIA
GENETIC BASICS OF VARIATIONS IN BACTERIA

... D. Genetic changes occurring within a single cell. Point mutations, single nucleotide changes in the DNA, can have a number of consequences. In coding regions they may alter an amino acid in a polypeptide. The effect may be deleterious (inactivation or lower activity) or beneficial (enhanced or new ...
Slides
Slides

... Yeast genome has 6000 genes Select 50 genes believed to be co-regulated by a common TF Found a motif for these 50 genes It appeared in 20 out of these 50 genes In the whole genome, 100 genes have this motif ...
Adobe PDF - VCU Secrets of the Sequence
Adobe PDF - VCU Secrets of the Sequence

... over in all species of animals, even sea creatures. This video explains how a complex set of genes called homeotic genes have been found in all these species. Researchers have discovered that these genes are turned on and off in different parts of the body thus controlling how skeletons are formed a ...
doc - VCU Secrets of the Sequence
doc - VCU Secrets of the Sequence

... over in all species of animals, even sea creatures. This video explains how a complex set of genes called homeotic genes have been found in all these species. Researchers have discovered that these genes are turned on and off in different parts of the body thus controlling how skeletons are formed a ...
Chromosome structure and mutations
Chromosome structure and mutations

... Many TEs sustain deletions during transposition or repair If promoter needed for transcription is deleted, TE can not transpose again Nonautonomous elements – need activity of intact copies of same TE for movement Autonomous elements – move by themselves Most SINEs and LINEs in human genome are defe ...
Supplementary Text 1 (doc 52K)
Supplementary Text 1 (doc 52K)

... Myxococcales based on 16S rRNA gene sequence similarity was calculated with sequences of at least 1300 bp length. A bootstrap analysis was derived from 2000 replicates. Shorter sequences were added later with maximum parsimony. Selected members of the Cyanobacteria were used as outgroup (not shown) ...
Alternative Splicing Analysis Tools Through the UCSC Genome
Alternative Splicing Analysis Tools Through the UCSC Genome

... 2. On the left, click on "Get Data"  "UCSC Main table browser". 3. On the right, select genome: "Human", assembly: "Mar. 2006", group: "Genes and Gene Prediction Tracks", track:"Alt Events". Click on "Describe table schema" to view the structure of the Alt Events track. In the upper table, click on ...
Chapter 5
Chapter 5

... genome contributed by the germ cells, egg and sperm, contains a large repertoire of immunoglobulin genes 2. Somatic-variation theory: maintained that the genome contains a small number of immunoglobulin genes, from which a large number of Ab specificities are generated in the somatic cells by mutati ...
Lecture 4
Lecture 4

... Two phase integration mechanism was proposed. In the “pre-integration” phase, transforming plasmid molecules (intact or partial) are spliced together. This gives rise to rearranged sequence, which upon integration don’t contain interspersed host DNA. Subsequently, integration of transgenic DNA into ...
Text S1.
Text S1.

... developmental defects in transgenic plants that express RNAi suppressors during development [1,2]. This effect may be due to convergence of the antiviral RNAi and miRNA pathways on Argonaute-1 (AGO1) in plants. In Drosophila, the miRNA and siRNA pathways are parallel pathways. Nevertheless, there is ...
MB207Jan2010
MB207Jan2010

... 1. Binding of transposase protein to transposon sequence. 2. Transposon DNA is replicated and a copy is inserted at a new chromosomal site, leaving the original chromosome intact. ...
Conditional (if else) lecture
Conditional (if else) lecture

... • A Hash table can be often used like an reference index ; e.g. “code of life” translation table : – hash_base.pl shows what the nucleotide base letter stands for. – Moreover Hash tables could be use, as it the exercise, to create a DNA codon conversion table so that when a codon is encountered as i ...
Comparisons of Maize pericarp color1 Alleles
Comparisons of Maize pericarp color1 Alleles

... 5). The general structure of the P1-rw1077 allele is similar to that of P1-rr4B2 (e.g., it has a single coding sequence flanked by two long [6.3 kb] direct repeats). However, the P1-rw1077 coding sequence is chimeric, consisting of a p1-like 59 UTR followed by p2-like exons and introns. Farther down ...
Eukaryotic Transcription
Eukaryotic Transcription

... Following the formation of the preinitiation complex, the polymerase is released from the other transcription factors, and elongation is allowed to proceed as it does in prokaryotes with the polymerase synthesizing premRNA in the 5' to 3' direction. As discussed previously, RNA polymerase II transcr ...
length of exons and introns in genes of some human chromosomes
length of exons and introns in genes of some human chromosomes

... including 1 to 11 genes per region of chromosome 1 (average value was 4 genes/Mbp) exon length decreased from 282 to 135 nt, as well as the number of introns in genes (Nin) increased. The average total exon lengths (Lex) in genes increased from 691 to 3163 nt and the positive correlation between Nin ...
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Transposable element



A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.
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