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Intrdouction to Annotation (djs)
Intrdouction to Annotation (djs)

... GeneMark, or GeneMark Smeg. Start sites are chosen to include all coding potential. These are, by far, the strongest pieces of data for predicting genes. 5. If there are two genes transcribed in opposite directions whose start sites are near one another, there typically has to be space between them ...
31_operons
31_operons

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Answers questions chapter 12
Answers questions chapter 12

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Example of BLASTN output
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...  LINES and SINES comprise a major part of the animal genome. The LINES comprise long interspersed sequences, and the SINES comprise short interspersed sequences (or interspersed repeats).  Plants contain another type of small mobile element, called MITE (for miniature inverted-repeat transposable ...
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Minos, a new transposable element from Drosophila hydei, is a

... was found inserted within the external transcribed spacer the rDNA locus, between bases 4257 and 4258 of the published sequence (2) (Figure 1). Southern blots of restricted DNA from two D. hydei strains showed distinct banding patterns (Figure 1), suggesting that the element is, or has until recentl ...
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Mutations - Department of Statistics | Rajshahi University

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投影片 1

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... DNA molecule will be: 1/4 x1/4 x 1/2 x 1/4 x 1/4 = 1/512; or it will cut once every 512 base pairs. The haploid genome of the fish is (3.8 x 106); expecting a cut every 512 nucleotides would produce (3.8 x 106)/512 or 7.4 x 105 fragments. Ans: (c). 2. Inserting a foreign DNA fragment into the BglI s ...
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Comparative genomics and Target discovery

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ASviewer: Visualizing the transcript structure and functional
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... Summary: Alternative splicing (AS) produces diverse transcript structures by differential use of splice sites. Comparing the gene structure and functional domains of splice variants is an essential but nontrivial task with numerous gene predictions available publicly. We developed a novel viewer (AS ...
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Transposable element



A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.
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