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Enhancement of antioxidant properties of tea extract and cellular
Enhancement of antioxidant properties of tea extract and cellular

... in alignment with the extensive metabolism of phenolic profile by LAB and elevation in total phenolic content compared with non-fermented samples. In addition, higher amount of TFLs from fermented TEs was taken up by Caco-2 monolayers, with the major constituents being catechins, gallic acid, querce ...
Recombination and Genetic Engineering
Recombination and Genetic Engineering

... Insertion sequences mediate a variety of DNA rearrangements. One of the first recognitions of this fact was the involvement of insertion sequences in the integration of F and R plasmids into the host chromosome. This event gives rise to Hfr strains. The initial DNA rearrangement mediated by IS eleme ...
Tandem repeats - Trimble County Schools
Tandem repeats - Trimble County Schools

... What is short tandem repeat and why is it so attractive to forensic scientists? How does the number of STRs characterized relate to the frequency of occurrence of the analyzed sample in the general population? (p. 380) Name two process by which a forensic scientist can separate STRs for characteriza ...
Mamm_Genome yTrx1-2 + refs
Mamm_Genome yTrx1-2 + refs

... the Trx12 sequence is flanked by a 15 bp direct repeat (with only one mismatch) that is believed to play a role in the insertion of the sequence into the genome (Vanin 1985). Fifth, the promoter regions described for human Trx1 (TATA box and SP1 binding site) have been replaced in Trx1-2 sequence, ...
13.2 Notes - Trimble County Schools
13.2 Notes - Trimble County Schools

... What is short tandem repeat and why is it so attractive to forensic scientists? How does the number of STRs characterized relate to the frequency of occurrence of the analyzed sample in the general population? (p. 380) Name two process by which a forensic scientist can separate STRs for characteriza ...
chromosomes
chromosomes

... DOMINANT or RECESSIVE. • Dominant genes are passed down even if there is only one copy of that gene in the pair. • For a recessive gene to be passed down, the person must have that gene on both chromosomes of the pair. ...
The first midterm will consist of 20 four
The first midterm will consist of 20 four

... 6. A 7. D 8. A 9. A 10. D 11. C 12. B Definitions 1. A codon; three base pairs in sequence that code for an amino acid (or stop signal). 2. Linkage analysis searching for linkages of small effect size of multiple genes at many loci. 3. Containing foreign DNA; e.g., inserting DNA from one organism in ...
Eucharyotic Chromatin Organization
Eucharyotic Chromatin Organization

...  3) moderately repetitive DNA makes many copies of itself while not affecting the rest of the chromosome. ...
Science Media Centre Fact Sheet Genome editing
Science Media Centre Fact Sheet Genome editing

... Once a break is made in the genome at the desired position the DNA repair mechanisms of a cell are triggered which can be harnessed to make the desired changes via two mechanisms: Homologous Recombination involves introducing a DNA fragment as a template for repair which contains the desired genetic ...
19. IMG-ER Curation Environment
19. IMG-ER Curation Environment

... EC number and PUBMED ID – see explanation Notes are free text (goes to “note” in GenBank submission) Gene symbol is “gene name” – 4 letter abbreviation; goes to “gene” in GenBank submission ...
Protein-coding genes in eukaryotic DNA
Protein-coding genes in eukaryotic DNA

... -- blocks of about 1 kilobase to 300 kb that are copied intra- or interchromosomally (5% of human genome) 5. Blocks of tandem repeats -- includes telomeric and centromeric repeats and can span millions bp (often species-specific) ...
Linking Genotype to Phenotype
Linking Genotype to Phenotype

... Lindsay MA, Nature Reviews Drug Discovery 2, 831-838 ...
structure and function of genome
structure and function of genome

... A transposon that inserts itself into a functional gene will most likely disable that gene. After a transposon leaves a gene, the resulting gap will probably not be repaired correctly. Multiple copies of the same sequence, such as Alu sequences can hinder precise chromosomal pairing during mitosis a ...
Red line lesson sketch
Red line lesson sketch

... First, use DNA subway to show how we can reveal features of a sequence. Create a project using a sample sequence. Once students have mastery, they can come back and create their own projects using real data. ...
Summer 2006
Summer 2006

... their compact genomes and minimal non-coding regions1. The advancement in computational genome analysis has permitted a more thorough examination of DNA sequences. As a result, it is now clear that repeat sequences are widespread in prokaryotic genomes. Short interspersed repeats are widely distribu ...
Microarray technique and Functional genomics
Microarray technique and Functional genomics

... Microarray terminology • Feature - an array element • Probe - a feature corresponding to a defined sequence (immobilized on a solid surface in an ordered array) • Target - a pool of nucleic acids of unknown sequence ...
Passarge, E. Taschenatlas der Genetik
Passarge, E. Taschenatlas der Genetik

... chromosomes: the nucleosomes, DNA in chromosomes, polytene chromosomes, the telomere, karyotype, chromosomal aberration, fluorescence in situ hybridization, translocation, molecular cytogenetic analysis, and others. Concluding chapter provides coverage of regulation of genes: the cell nucleus and ri ...
DYNC2H1 Clipson Family Variants 27.11.09 1.I2526S/N c.7577T>G
DYNC2H1 Clipson Family Variants 27.11.09 1.I2526S/N c.7577T>G

...  Jeune asphyxiating thoracic dystrophy is a skeletal dysplasia caused by mutations in the IFT80 gene that encodes a protein involved in intraflagellar transport of primary cilia (Beales et al 2007 Nature Genetics) ...
Presenter 18 - Florida International University
Presenter 18 - Florida International University

... Largest known human gene is dystrophin at 2.4 million bases. Chromosome 21 is the smallest human chromosome. Three copies of this autosome causes Down syndrome, the most frequent genetic disorder associated with significant mental ...
2054, Chap. 13, page 1 I. Microbial Recombination and Plasmids
2054, Chap. 13, page 1 I. Microbial Recombination and Plasmids

... b. most important for integration of viral genomes into bacterial chromosomes c. transposons use a type of site-specific recombination called replicative recombination 4. horizontal gene transfer = transfer of genetic material from one mature individual to another a. common in bacteria (think antibi ...
Ways to get from plant genomes to phenomes: via
Ways to get from plant genomes to phenomes: via

... of Toronto, Canada). Out of the 6,000 yeast genes, 5,000 have been shown to be non-essential in a genome-wide single-gene-knockout project, but double mutants of these non-essential genes often have lethal phenotypes (synthetic lethal phenotypes). SGA analysis allows the identification of genetic in ...
Human Genome
Human Genome

... in the GC rich regions and that these ‘selfish’ elements may benefit their human hosts 8. The mutation rate is about twice as high in maleas in female meiosis. Thus, most mutation occurs in males 9. Large GC-poor regions are strongly correlated with ‘dark G-bands’ in karyotypes ...
04/01
04/01

... Relationship between Genotype and Phenotype ...
Developing a new genetic system in bacteria
Developing a new genetic system in bacteria

... • More realistic possibility today than ever before, especially with 454 sequencing. • Useful for – Locating potentially important genes (by homology) – Mapping genes you find by other methods (eg, cloning, transposon mutatenesis) – find linked genes that may be involved in your process – Microarray ...
Group 4 members
Group 4 members

... – Haploid genetic screens in human cells identify host factors used by pathogens. Science, November 27, 2009. – Ebola virus entry requires the cholesterol transporter NiemannPick C1. Nature, online on August 24, 2011. ...
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Transposable element



A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.
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