Paper - Ran Blekhman
... typically short, degenerate, and therefore difficult to differentiate from randomly occurring, nonfunctional sequences. Furthermore, although sites such as TFBSs can be computationally predicted using evolutionary conservation as a criterion, estimates of the true level of selective constraint (defi ...
... typically short, degenerate, and therefore difficult to differentiate from randomly occurring, nonfunctional sequences. Furthermore, although sites such as TFBSs can be computationally predicted using evolutionary conservation as a criterion, estimates of the true level of selective constraint (defi ...
The genomic landscape of chronic lymphocytic leukemia: clinical
... The results of this study suggest for the first time that LC-MBL and HC-MBL are biologically distinct entities and suggest that the increasing BcR stereotypy observed in larger clones may underpin their different clinical behaviour. It would however also be possible to contend that LC-MBL may in fa ...
... The results of this study suggest for the first time that LC-MBL and HC-MBL are biologically distinct entities and suggest that the increasing BcR stereotypy observed in larger clones may underpin their different clinical behaviour. It would however also be possible to contend that LC-MBL may in fa ...
Varicella-Zoster Virus Specifies a Thymidylate Synthetase
... was isolated from a plasmid containing VZV EcoRI m and ligated to appropriately cleaved pKK240-11. The ligated D N A was used to transform E. coli strain X2913 (obtained from Dr B. Bachmann, E. coli Genetic Stock Centre) to ampicillin resistance. The host strain cannot grow on minimal agar medium la ...
... was isolated from a plasmid containing VZV EcoRI m and ligated to appropriately cleaved pKK240-11. The ligated D N A was used to transform E. coli strain X2913 (obtained from Dr B. Bachmann, E. coli Genetic Stock Centre) to ampicillin resistance. The host strain cannot grow on minimal agar medium la ...
FAN: fingerprint analysis of nucleotide sequences
... E-value of one for a database the size of PRINTS—not surprisingly, the fingerprint is unrelated. The sequence shown in the figure has a frameshift-causing sequence error; however, because it does not occur within a motif-matching region, it has had no effect on the significance of the match. The rel ...
... E-value of one for a database the size of PRINTS—not surprisingly, the fingerprint is unrelated. The sequence shown in the figure has a frameshift-causing sequence error; however, because it does not occur within a motif-matching region, it has had no effect on the significance of the match. The rel ...
Comparisons of Maize pericarp color1 Alleles
... P1-wr (Zhang et al., 2000); partial sequence analysis of these clones indicated that they were derived from p2 (Zhang et al., 2003). The second class hybridizes with both probes 15 and 8B and has the 80-bp sequence in the 59 UTR sequence; thus, it appears to contain the p1 gene. Two overlapping p1-c ...
... P1-wr (Zhang et al., 2000); partial sequence analysis of these clones indicated that they were derived from p2 (Zhang et al., 2003). The second class hybridizes with both probes 15 and 8B and has the 80-bp sequence in the 59 UTR sequence; thus, it appears to contain the p1 gene. Two overlapping p1-c ...
PTC Genetics Lab Student Worksheet
... The sensation of taste can be categorized into five basic types: sweet, sour, salty, bitter, and umami (the taste of monosodium glutamate). These five tastes serve to classify compounds into potentially nutritive and beneficial (sweet, salty, umami) or potentially harmful or toxic (bitter, sour). Th ...
... The sensation of taste can be categorized into five basic types: sweet, sour, salty, bitter, and umami (the taste of monosodium glutamate). These five tastes serve to classify compounds into potentially nutritive and beneficial (sweet, salty, umami) or potentially harmful or toxic (bitter, sour). Th ...
Construction of nanA mutants
... each case. The 1500 bp Janus Cassette was amplified with primers Janus KpnF and Janus SacIR. The PCR products generated from the 3 individual reactions were then cleaned and digested with the appropriate enzymes, cleaned again, and then ligated. The ligation mix was used as template for an extended ...
... each case. The 1500 bp Janus Cassette was amplified with primers Janus KpnF and Janus SacIR. The PCR products generated from the 3 individual reactions were then cleaned and digested with the appropriate enzymes, cleaned again, and then ligated. The ligation mix was used as template for an extended ...
Chapter 25 DNA metabolism
... Are specific for DNA not RNA Two major classes Exonucleases nibble in from end May be 5' or 3' but not both Endonucleases start somewhere in the middle Endonuclease that attack specific sequences are called restriction enzymes A few endo and exo’s only work on single stranded DNA Interestingly enoug ...
... Are specific for DNA not RNA Two major classes Exonucleases nibble in from end May be 5' or 3' but not both Endonucleases start somewhere in the middle Endonuclease that attack specific sequences are called restriction enzymes A few endo and exo’s only work on single stranded DNA Interestingly enoug ...
Two Anthranilate Synthase Genes in Arabidopsis
... A fragment of the yeast TRP2 gene (Zalkin et al., 1984) containing the region most highly conserved between yeast and bacterial AS a subunit genes was used as a DNA hybridization probe to isolate homologous cDNAs from Arabidopsis. A cDNA with 500/0 nucleotide and 39% amino acid identity to the yeast ...
... A fragment of the yeast TRP2 gene (Zalkin et al., 1984) containing the region most highly conserved between yeast and bacterial AS a subunit genes was used as a DNA hybridization probe to isolate homologous cDNAs from Arabidopsis. A cDNA with 500/0 nucleotide and 39% amino acid identity to the yeast ...
Spr01Final Exam Answer Key
... morphology. Suppose you were trying to isolate rII mutants. After you mutagenize your phage, would you infect E.coli or E.coli λ to screen for your mutants? Why? (4pts) E.coli, because the mutants you are hoping to isolate would not grow in E.coli λ. After isolating several mutants, Benzer then trie ...
... morphology. Suppose you were trying to isolate rII mutants. After you mutagenize your phage, would you infect E.coli or E.coli λ to screen for your mutants? Why? (4pts) E.coli, because the mutants you are hoping to isolate would not grow in E.coli λ. After isolating several mutants, Benzer then trie ...
26 Fungal Genetics Newsletter Michelle Dequard-Chablat and Philippe Silar
... cerevisiae S28 protein. At least one orthologue is present in the complete genomes of all organisms sequenced to date and the gene is highly conserved in fungi (figure 1). For example, the P. anserina and Neurospora crassa proteins are 99% identical . Pa_3_5360 is located on the right arm of chromos ...
... cerevisiae S28 protein. At least one orthologue is present in the complete genomes of all organisms sequenced to date and the gene is highly conserved in fungi (figure 1). For example, the P. anserina and Neurospora crassa proteins are 99% identical . Pa_3_5360 is located on the right arm of chromos ...
Gene duplications in prokaryotes can be
... Additional File 1), see Methods. Among these organisms were 10 archaea. The paralog fraction was defined as the ratio between the number of proteins with one or more paralogs (not considering the number of copies) and the total number of proteins in that particular proteome, and is also known as the ...
... Additional File 1), see Methods. Among these organisms were 10 archaea. The paralog fraction was defined as the ratio between the number of proteins with one or more paralogs (not considering the number of copies) and the total number of proteins in that particular proteome, and is also known as the ...
Molecular analysis of extracellular-superoxide dismutase
... and hemodialysis patients. The distribution of the serum EC-SOD contents was discontinuous in healthy persons (n= 103), and the contents were clearly separated into two groups; a lower levels (group I) below 400 ng/ml and a higher levels (group II) above 600 ng/ml, as described previously (Adachi et ...
... and hemodialysis patients. The distribution of the serum EC-SOD contents was discontinuous in healthy persons (n= 103), and the contents were clearly separated into two groups; a lower levels (group I) below 400 ng/ml and a higher levels (group II) above 600 ng/ml, as described previously (Adachi et ...
Linkage, Crossing Over, and Chromosome Mapping
... FISH Chromosome Painting Using Probes With Cloned Libraries ...
... FISH Chromosome Painting Using Probes With Cloned Libraries ...
gyrA AND SEQUENCING METHOD
... observed commonly in the regions where fluoroquinolones are used empirically to treat enteric fever. Quinolone and fluoroquinoloneresistance commonly arise via mutations in the genes encoding DNA gyrase (gyr A and gyr B) and DNA topoisomerase IV (par C and par E) [5]. A number of resistance mechanis ...
... observed commonly in the regions where fluoroquinolones are used empirically to treat enteric fever. Quinolone and fluoroquinoloneresistance commonly arise via mutations in the genes encoding DNA gyrase (gyr A and gyr B) and DNA topoisomerase IV (par C and par E) [5]. A number of resistance mechanis ...
Understanding the Human Karyotype - Dr. Jackson
... High resolution (HR) banding by altering the chromosome condensation cycle, very long and extended chromosomes may be obtained with as many as 850+ bands per haploid set. This allows for a very detailed analysis of small aberrations. ...
... High resolution (HR) banding by altering the chromosome condensation cycle, very long and extended chromosomes may be obtained with as many as 850+ bands per haploid set. This allows for a very detailed analysis of small aberrations. ...
8.1 Human Chromosomes and Genes
... Mendelian Inheritance in Humans Mendelian inheritance refers to the inheritance of traits controlled by a single gene with two alleles, one of which may be dominant to the other. Not many human traits are controlled by a single gene with two alleles, but they are a good starting point for understand ...
... Mendelian Inheritance in Humans Mendelian inheritance refers to the inheritance of traits controlled by a single gene with two alleles, one of which may be dominant to the other. Not many human traits are controlled by a single gene with two alleles, but they are a good starting point for understand ...
Positional dependence of transcriptional inhibition by DNA torsional
... became the predominant over-represented category (Supplementary Table I). Physical clustering of altered genes on accumulation of DNA helical stress To examine how transcriptome alterations between the above top2ts and TOP2 strains spread throughout the yeast chromosomes after the accumulation of DN ...
... became the predominant over-represented category (Supplementary Table I). Physical clustering of altered genes on accumulation of DNA helical stress To examine how transcriptome alterations between the above top2ts and TOP2 strains spread throughout the yeast chromosomes after the accumulation of DN ...
An interspecific plant hybrid shows novel changes in
... Alternative splicing is a fundamental aspect of the expression of many genes. However alternative splicing patterns have not been examined in multiple genes in an interspecific plant hybrid compared with its parents. Here we studied alternative splicing patterns in an interspecific Populus hybrid an ...
... Alternative splicing is a fundamental aspect of the expression of many genes. However alternative splicing patterns have not been examined in multiple genes in an interspecific plant hybrid compared with its parents. Here we studied alternative splicing patterns in an interspecific Populus hybrid an ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.