$doc.title
... and the pNL4-‐3 wt plasmid were then digested with both AgeI and EcoRI (New England Biolabs). In order to confirm the insertion of the BstEII restriction site, the PCR pNL4-‐3.AgeIEcoRI was ...
... and the pNL4-‐3 wt plasmid were then digested with both AgeI and EcoRI (New England Biolabs). In order to confirm the insertion of the BstEII restriction site, the PCR pNL4-‐3.AgeIEcoRI was ...
The Relationship Between DNA Replication and the
... termination of DNA replication during sporulation If the rate of DNA replication in a resuspended culture were reduced, one would expect that the time of chromosome termination, and hence the time of onset of escape from the effect of HPUra, would be delayed. One would also expect the resulting rate ...
... termination of DNA replication during sporulation If the rate of DNA replication in a resuspended culture were reduced, one would expect that the time of chromosome termination, and hence the time of onset of escape from the effect of HPUra, would be delayed. One would also expect the resulting rate ...
Document
... Mammalian and other vertebrate mtDNAs are transcribed as a single large RNA molecule (polycistronic) and cleaved to produce mRNAs, tRNAs, and rRNAs before they are processed. ...
... Mammalian and other vertebrate mtDNAs are transcribed as a single large RNA molecule (polycistronic) and cleaved to produce mRNAs, tRNAs, and rRNAs before they are processed. ...
chapter_13b
... Mammalian and other vertebrate mtDNAs are transcribed as a single large RNA molecule (polycistronic) and cleaved to produce mRNAs, tRNAs, and rRNAs before they are processed. ...
... Mammalian and other vertebrate mtDNAs are transcribed as a single large RNA molecule (polycistronic) and cleaved to produce mRNAs, tRNAs, and rRNAs before they are processed. ...
Discriminate the Falsely Predicted Protein–Coding Genes in
... lacking of introns, whereas more and more researches indicate the issue of gene finding in microbial genomes is far from thoroughly resolved, the annotation quality of microbial genomes has been questioned continuously [1, 2]. In most microbial genomes, it is found some annotated genes do not encode ...
... lacking of introns, whereas more and more researches indicate the issue of gene finding in microbial genomes is far from thoroughly resolved, the annotation quality of microbial genomes has been questioned continuously [1, 2]. In most microbial genomes, it is found some annotated genes do not encode ...
Reassignment of the Human CSFl Gene to Chromosome lp13-p21
... labeling of 21 of 32 chromosome 1 chromatids at this position was seen in eight representative metaphases selected for photography. Hybridization with an independently derived genomic clone, pHCSF-la, also containing the complete CSFl coding sequence, yielded an identical pattern of fluorescence. A ...
... labeling of 21 of 32 chromosome 1 chromatids at this position was seen in eight representative metaphases selected for photography. Hybridization with an independently derived genomic clone, pHCSF-la, also containing the complete CSFl coding sequence, yielded an identical pattern of fluorescence. A ...
model for Escherichia coli chromosome packaging supports
... lead to organization of highly condensed and confined circular chromosomes? One of us previously measured and analyzed the position fluctuations of 15 single genetic loci (in G and early S phase), among which are the origin of replication OriC as well as two loci named ‘lac’ and ‘C4’ for convenience [ ...
... lead to organization of highly condensed and confined circular chromosomes? One of us previously measured and analyzed the position fluctuations of 15 single genetic loci (in G and early S phase), among which are the origin of replication OriC as well as two loci named ‘lac’ and ‘C4’ for convenience [ ...
Semiconservative Replication in the Quasispecies Model
... not necessarily paired with σ̄. Rather, once cell division is finished, the original σ is paired with some σ ′ , and similarly for σ̄. Each genome {σ, σ̄} has a characteristic replication mismatch probability ǫ{σ,σ̄} (a base-pair-independent mismatch probability is certainly a simplification, but it ...
... not necessarily paired with σ̄. Rather, once cell division is finished, the original σ is paired with some σ ′ , and similarly for σ̄. Each genome {σ, σ̄} has a characteristic replication mismatch probability ǫ{σ,σ̄} (a base-pair-independent mismatch probability is certainly a simplification, but it ...
Do nonasterid holoparasitic flowering plants have plastid genomes?
... products, and sequencing conditions have been described [27]. The PCR products from Cytinus and Hydnora were sequenced directly. Insufficient product was obtained from Corynaea crassa after the amplification of the 1.2 kb fragment to allow direct sequencing. This product was cloned prior to sequenci ...
... products, and sequencing conditions have been described [27]. The PCR products from Cytinus and Hydnora were sequenced directly. Insufficient product was obtained from Corynaea crassa after the amplification of the 1.2 kb fragment to allow direct sequencing. This product was cloned prior to sequenci ...
Functional Analysis of the Genes of Yeast Chromosome V by Genetic Footprinting.
... (encoding a RecA-like DNA repair protein), PRB1 (encoding vacuolar protease B), and GLN3 (encoding a positive nitrogen-regulatory protein) (12). Spurious PCR products (those unrelated to real Ty1 insertions in the gene of interest) would not be expected to be depleted under a selection that requires ...
... (encoding a RecA-like DNA repair protein), PRB1 (encoding vacuolar protease B), and GLN3 (encoding a positive nitrogen-regulatory protein) (12). Spurious PCR products (those unrelated to real Ty1 insertions in the gene of interest) would not be expected to be depleted under a selection that requires ...
UBE3B developmental disorders and increased mortality in cattle
... Loss-of-function mutations in UBE3B cause severe neurodevelopmental disorders in humans such as developmental delay, intellectual disability and characteristic facial dysmorphisms, e.g., ptosis, blepharophimosis and telecanthus. Affected individuals also suffer from severe growth retardation, hypoto ...
... Loss-of-function mutations in UBE3B cause severe neurodevelopmental disorders in humans such as developmental delay, intellectual disability and characteristic facial dysmorphisms, e.g., ptosis, blepharophimosis and telecanthus. Affected individuals also suffer from severe growth retardation, hypoto ...
Chapter 3
... tremendous effect on biology and our society. Genetic mechanisms explain how traits are passed between generations. They also help explain how species change over time. Genetic and evolutionary themes are interdependent in biology, and biology without either would be unrecognizable from its present ...
... tremendous effect on biology and our society. Genetic mechanisms explain how traits are passed between generations. They also help explain how species change over time. Genetic and evolutionary themes are interdependent in biology, and biology without either would be unrecognizable from its present ...
Organization and dynamics of plant interphase chromosomes
... positions [24,50,51]. Sister chromatid alignment is independent of the CpG methylation level, because separation of sister chromatids in the hypomethylation mutant ddm1 [52] was no more frequent than in the wild-type background [50]. An Arabidopsis mutant of the p150 subunit of the chromatin assembl ...
... positions [24,50,51]. Sister chromatid alignment is independent of the CpG methylation level, because separation of sister chromatids in the hypomethylation mutant ddm1 [52] was no more frequent than in the wild-type background [50]. An Arabidopsis mutant of the p150 subunit of the chromatin assembl ...
Archives of Microbiology 167:
... carry out N2 fixation only when the oxygen tension in the environment is low, e.g., during the dark phase of a natural diel cycle when photosynthetic O2 production ceases and respiratory O2 consumption increases (Gallon 1992). Many filamentous cyanobacteria fix N2 concurrently with photosynthetic O2 ...
... carry out N2 fixation only when the oxygen tension in the environment is low, e.g., during the dark phase of a natural diel cycle when photosynthetic O2 production ceases and respiratory O2 consumption increases (Gallon 1992). Many filamentous cyanobacteria fix N2 concurrently with photosynthetic O2 ...
1 X chromosome crossover formation and genome stability in
... The germ line efficiently combats numerous genotoxic insults to ensure the high fidelity propagation of unaltered genomic information across generations. Yet, germ cells in most metazoans also intentionally create double-strand breaks (DSBs) to promote DNA exchange between parental chromosomes, a pr ...
... The germ line efficiently combats numerous genotoxic insults to ensure the high fidelity propagation of unaltered genomic information across generations. Yet, germ cells in most metazoans also intentionally create double-strand breaks (DSBs) to promote DNA exchange between parental chromosomes, a pr ...
Why Gene Duplication? ,
... free copies of the nucleolar organizer during oogenesis probably occurs on a much smaller scale, if it occurs at all. Nevertheless, the fact that a segment of the chromosome can engage in repeated DNA replication and disseminate its free copies, while the rest of the chromosomes are not involved in ...
... free copies of the nucleolar organizer during oogenesis probably occurs on a much smaller scale, if it occurs at all. Nevertheless, the fact that a segment of the chromosome can engage in repeated DNA replication and disseminate its free copies, while the rest of the chromosomes are not involved in ...
1. Chromatin structure is based on successive levels of DNA packing
... • Interphase chromatin is generally much less condensed than the chromatin of mitosis. – While the 30-nm fibers and looped domains remain, the discrete scaffold is not present. – The looped domains appear to be attached to the nuclear lamina and perhaps the nuclear matrix. ...
... • Interphase chromatin is generally much less condensed than the chromatin of mitosis. – While the 30-nm fibers and looped domains remain, the discrete scaffold is not present. – The looped domains appear to be attached to the nuclear lamina and perhaps the nuclear matrix. ...
A Comparative Genomic Analysis of Two Distant Diptera, the Fruit
... Chromosomal Regions In a first set of experiments aiming at exploring long-range synteny and microsynteny, we identified, among the currently available A. gambiae sequences, putative orthologs of genes in which in D. melanogaster are clustered within two well-studied chromosomal regions, each nearly ...
... Chromosomal Regions In a first set of experiments aiming at exploring long-range synteny and microsynteny, we identified, among the currently available A. gambiae sequences, putative orthologs of genes in which in D. melanogaster are clustered within two well-studied chromosomal regions, each nearly ...
Simplified Insertion of Transgenes Onto Balancer Chromosomes via
... during gene replacement by homologous recombination (Huang et al. 2008). Thus, a pattern exists in which the development of new genetic technologies consistently leads researchers to target new transgenes to Drosophila balancer chromosomes. For each of the examples listed above, transgenic insertion ...
... during gene replacement by homologous recombination (Huang et al. 2008). Thus, a pattern exists in which the development of new genetic technologies consistently leads researchers to target new transgenes to Drosophila balancer chromosomes. For each of the examples listed above, transgenic insertion ...
Duplication of an approximately 1.5 Mb DNA segment
... mitotic recombination at the molecular level is restricted to an approximate 150 kb DNA fragment between the APC and MCC genes at chromosome 5q22. We have shown in this study that the same recombination site is involved in the duplication of the smallest overlapping region of approximately 1.5 Mb DN ...
... mitotic recombination at the molecular level is restricted to an approximate 150 kb DNA fragment between the APC and MCC genes at chromosome 5q22. We have shown in this study that the same recombination site is involved in the duplication of the smallest overlapping region of approximately 1.5 Mb DN ...
Advanced Bacterial Conjugation Kit
... resistance to antibiotics is a type of genetic recombination that enables the new recombinant bacterial cell to express resistance to an antibiotic to which it was formerly sensitive. While bacterial chromosomes normally carry all the genes necessary for growth and reproduction, bacteria also contai ...
... resistance to antibiotics is a type of genetic recombination that enables the new recombinant bacterial cell to express resistance to an antibiotic to which it was formerly sensitive. While bacterial chromosomes normally carry all the genes necessary for growth and reproduction, bacteria also contai ...
Biology
... 39. Viruses use different strategies to survive. The lytic and lysogenic cycles illustrate two of them. (12pts) Compare the two strategies using the Venn diagram to place the words or phrases given into the diagram. prophage Viral DNA circularizes symptoms appear soon symptoms appear much later inf ...
... 39. Viruses use different strategies to survive. The lytic and lysogenic cycles illustrate two of them. (12pts) Compare the two strategies using the Venn diagram to place the words or phrases given into the diagram. prophage Viral DNA circularizes symptoms appear soon symptoms appear much later inf ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.