Active site amino acid sequence of the bovine O6
... other biological effects of alkylating agents. A number of human and rodent cell lines are unable to remove m6-Gua from their DNA. These cell lines are designated Mex~ (or Mer~) and are hypersensitive to the cytotoxic, clastogenic and mutagenic action of methylating agents (5,6). The hypersensitivit ...
... other biological effects of alkylating agents. A number of human and rodent cell lines are unable to remove m6-Gua from their DNA. These cell lines are designated Mex~ (or Mer~) and are hypersensitive to the cytotoxic, clastogenic and mutagenic action of methylating agents (5,6). The hypersensitivit ...
The connection between transcription and genomic instability
... region more susceptible to attack by internal metabolites that are reactive with ssDNA, leading to both mutagenic and recombinogenic lesions. This hypothesis has the advantage that it can also explain TAM, even though more quantitative data are lacking on TAM to strengthen this possibility. In addit ...
... region more susceptible to attack by internal metabolites that are reactive with ssDNA, leading to both mutagenic and recombinogenic lesions. This hypothesis has the advantage that it can also explain TAM, even though more quantitative data are lacking on TAM to strengthen this possibility. In addit ...
Pierce chapter 9
... – If crossing over occurs within loop: – Creates a dicentric chromosome and an acentric chromosome • Acentric is lost • Dicentric forms a dicentric bridge, and breaks • Nonviable recombinant ...
... – If crossing over occurs within loop: – Creates a dicentric chromosome and an acentric chromosome • Acentric is lost • Dicentric forms a dicentric bridge, and breaks • Nonviable recombinant ...
DNA Shape Dominates Sequence Affinity in Nucleosome Formation
... and local flexibility—that render them more or less favorable for protein binding and nucleosome formation. Recent studies of these structural properties have considered them individually, and several views exist of the physical origins of nucleosome positioning. One such view assumes that sequence ...
... and local flexibility—that render them more or less favorable for protein binding and nucleosome formation. Recent studies of these structural properties have considered them individually, and several views exist of the physical origins of nucleosome positioning. One such view assumes that sequence ...
doc
... emerging technologies, a selection of the most important are summarised below; Database-mining: this is the process by which the structure/function of an unknown gene/protein is inferred from similar sequences identified in information already stored in database, most often from well-characterised m ...
... emerging technologies, a selection of the most important are summarised below; Database-mining: this is the process by which the structure/function of an unknown gene/protein is inferred from similar sequences identified in information already stored in database, most often from well-characterised m ...
Syntrophic linkage between predatory Carpediemonas and
... with other microbes has rarely been investigated so far. Here, we studied an example of such an interaction by cultivating the anerobic marine flagellate, Carpediemonas frisia sp. nov. (supergroup Excavata), with parts of its naturally associated microbiome. This microbiome consisted of so far uncul ...
... with other microbes has rarely been investigated so far. Here, we studied an example of such an interaction by cultivating the anerobic marine flagellate, Carpediemonas frisia sp. nov. (supergroup Excavata), with parts of its naturally associated microbiome. This microbiome consisted of so far uncul ...
What is PCR? - Cobb Learning
... millions of copies of a specific DNA sequence in a relatively short period of time ...
... millions of copies of a specific DNA sequence in a relatively short period of time ...
Identification of Amino Acid Substitutions that Alter the Substrate Specificity of TEM-1 b-Lactamase.
... P-Lactam antibiotics such as penicillins and cephalosporins are among the most often used antimicrobial agents. Because of the selective pressure resulting from the prevalence of j-lactam antibiotic use, bacterial resistance has increased and now represents a serious threat to antibiotic therapy (14 ...
... P-Lactam antibiotics such as penicillins and cephalosporins are among the most often used antimicrobial agents. Because of the selective pressure resulting from the prevalence of j-lactam antibiotic use, bacterial resistance has increased and now represents a serious threat to antibiotic therapy (14 ...
BLAST- bioinformatics
... Multiple sequence alignments can help identify conserved regions of coding sequences, which might have functional significance, or to help understand evolutionary relationships among difficult to classify organisms. Multiple sequence alignments can also help with the development of primers in order ...
... Multiple sequence alignments can help identify conserved regions of coding sequences, which might have functional significance, or to help understand evolutionary relationships among difficult to classify organisms. Multiple sequence alignments can also help with the development of primers in order ...
Full Text
... al., 2016) as same species genes originated by a speciation event, which were brought back together in the same genome by allopolyploidization, but we plan to address these insufficiencies in future releases. The BLAST runs are performed in parallel for each sequence type and provide up to four time ...
... al., 2016) as same species genes originated by a speciation event, which were brought back together in the same genome by allopolyploidization, but we plan to address these insufficiencies in future releases. The BLAST runs are performed in parallel for each sequence type and provide up to four time ...
Genetics Workbook
... Calculate the coefficient of interference. 4. In Drosophila, three autosomal genes have the following map: a----20cM----b-10cMc Provide the data, in terms of the expected number of flies in the following phenotypic classes, when a+ b+ c+ / a b c females are crossed to a b c / a b c males. Assume ...
... Calculate the coefficient of interference. 4. In Drosophila, three autosomal genes have the following map: a----20cM----b-10cMc Provide the data, in terms of the expected number of flies in the following phenotypic classes, when a+ b+ c+ / a b c females are crossed to a b c / a b c males. Assume ...
FREE Sample Here
... 43. Draw how the dNTP is added to a DNA strand at the 3' end. Ans: The drawing should indicate clearly how the 3'OH oxygen attacks the α phosphate on the incoming dNTP with the displacement of pyrophosphate. Section: 4.4 and Figure 4.25 44. How does RNA polymerase differ from DNA polymerase? Ans: Al ...
... 43. Draw how the dNTP is added to a DNA strand at the 3' end. Ans: The drawing should indicate clearly how the 3'OH oxygen attacks the α phosphate on the incoming dNTP with the displacement of pyrophosphate. Section: 4.4 and Figure 4.25 44. How does RNA polymerase differ from DNA polymerase? Ans: Al ...
second of three for Chapter 8
... When an inversion involves the centromere, it is called a pericentric inversion. Crossing over in a pericentric inversion does not create dicentric and acentric chromosomes ...
... When an inversion involves the centromere, it is called a pericentric inversion. Crossing over in a pericentric inversion does not create dicentric and acentric chromosomes ...
Ethical issues raised by genetically modified microorganisms
... plasmids (small loops of DNA which are capable of replication independently of the chromosome) which replicate both in the donor and the recipient bacteria. These plasmids do not only contain transfer genes but also other genes which often allow the bacteria to adapt to an ecological niche (genes en ...
... plasmids (small loops of DNA which are capable of replication independently of the chromosome) which replicate both in the donor and the recipient bacteria. These plasmids do not only contain transfer genes but also other genes which often allow the bacteria to adapt to an ecological niche (genes en ...
Genetic and Epigenetic Aspects of Polyploid Evolution in Plants
... somatic chromosome number variation in mitotically dividing cells, leading to aneuploid mosaics and suggesting a mechanism for the generation of variation in offspring. These plants are, however, phenotypically mostly stable, and fertility and offspring viability are not affected by the somatic aneu ...
... somatic chromosome number variation in mitotically dividing cells, leading to aneuploid mosaics and suggesting a mechanism for the generation of variation in offspring. These plants are, however, phenotypically mostly stable, and fertility and offspring viability are not affected by the somatic aneu ...
... This table was originally taken from the website: http://www.uea.ac.uk/~b270/repair.htm, which was last modified in 2005. This version is from Dec. 2012. Please feel free to send me any updates/corrections- highlighted so that I can find them. The references are not complete- to get current referenc ...
... This table was originally taken from the website: http://www.uea.ac.uk/~b270/repair.htm, which was last modified in 2005. This version is from Dec. 2012. Please feel free to send me any updates/corrections- highlighted so that I can find them. The references are not complete- to get current referenc ...
Multifractal analysis of DNA sequences using a novel chaos
... the decay of correlations in the DNA sequence by Gxing a scale k = 1=2L , that corresponds to subsequences of length L, and by computing directly the spectrum fL () at that scale using (6). The way in which the spectrum varies provides a quantiGcation of the non-random structures existing within th ...
... the decay of correlations in the DNA sequence by Gxing a scale k = 1=2L , that corresponds to subsequences of length L, and by computing directly the spectrum fL () at that scale using (6). The way in which the spectrum varies provides a quantiGcation of the non-random structures existing within th ...
DNA and Its Role in Heredity
... DNA polymerases can make mistakes in replication, but most errors are repaired. Cells have two major repair mechanisms: ...
... DNA polymerases can make mistakes in replication, but most errors are repaired. Cells have two major repair mechanisms: ...
MCDB 1041 Activity 3: Thinking about how “linkage” affects the
... In the 1930’s, scientists had the idea that they could figure out where all the genes were on the human chromosomes by following patterns of inheritance. They looked for diseases or traits that were commonly inherited together as an indication that the two genes causing the diseases were located clo ...
... In the 1930’s, scientists had the idea that they could figure out where all the genes were on the human chromosomes by following patterns of inheritance. They looked for diseases or traits that were commonly inherited together as an indication that the two genes causing the diseases were located clo ...
Prokaryotes: The First Life on Earth
... double-stranded, circular structure containing the prokaryote's DNA. Prokaryotes usually only have one chromosome and the area where it is located is known as the nucleoid. http://www.singleton-associates.org/bacteri2.htm ...
... double-stranded, circular structure containing the prokaryote's DNA. Prokaryotes usually only have one chromosome and the area where it is located is known as the nucleoid. http://www.singleton-associates.org/bacteri2.htm ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.