A novel DNA modification by sulphur
... Methylation is the most common covalent modification of DNA so far reported (Luria and Human, 1952; Pradhan et al., 1999), although some bacteriophages modify their DNA in other ways (Hattman, 1979; 1980; Swinton et al., 1983). An unusual and seemingly novel DNA modification was discovered in the Gr ...
... Methylation is the most common covalent modification of DNA so far reported (Luria and Human, 1952; Pradhan et al., 1999), although some bacteriophages modify their DNA in other ways (Hattman, 1979; 1980; Swinton et al., 1983). An unusual and seemingly novel DNA modification was discovered in the Gr ...
Supplementary Information (docx 2885K)
... fragment was end repaired and polyA was ligated to the 3'and 5' end and Illumina adapters. The size selected product was PCR amplified, and the final product was validated using the Agilent Bioanalyzer. Two steps of hybridization and wash were needed for construction. PCR was used to amplify the enr ...
... fragment was end repaired and polyA was ligated to the 3'and 5' end and Illumina adapters. The size selected product was PCR amplified, and the final product was validated using the Agilent Bioanalyzer. Two steps of hybridization and wash were needed for construction. PCR was used to amplify the enr ...
Phylogenetic Affinity of Mitochondria of Euglena
... even after the introduction of strand breakage by g-ray irradiation of the agarose blocks prior to electrophoresis. As a control for the extent of strand breakage by the g-irradiation, the circular chloroplast DNA was found to migrate through the CHEF gel as 140-kb linear fragments (data not shown). ...
... even after the introduction of strand breakage by g-ray irradiation of the agarose blocks prior to electrophoresis. As a control for the extent of strand breakage by the g-irradiation, the circular chloroplast DNA was found to migrate through the CHEF gel as 140-kb linear fragments (data not shown). ...
SNPs - Biology, Genetics and Bioinformatics Unit
... Spinocerebellar ataxia Type10 (SCA10) (OMIM:+603516) is caused by largest tandem repeat seen in human genome. Normal population has 10-22 mer pentanucleotide ATTCT repeat in intron 9 of SCA10 gene; where as SCA10 patients have 800-4500 repeat units, which causes the disease allele up to 22.5 kb larg ...
... Spinocerebellar ataxia Type10 (SCA10) (OMIM:+603516) is caused by largest tandem repeat seen in human genome. Normal population has 10-22 mer pentanucleotide ATTCT repeat in intron 9 of SCA10 gene; where as SCA10 patients have 800-4500 repeat units, which causes the disease allele up to 22.5 kb larg ...
Adobe PDF - Boston University Physics
... be approximated by power-law functions, while for coding DNA, they can be well fit by a firstorder Markov process. We propose a model, based on known biophysical processes, which leads to the observed probability distribution functions for noncoding DNA. We argue that this difference in the shape of ...
... be approximated by power-law functions, while for coding DNA, they can be well fit by a firstorder Markov process. We propose a model, based on known biophysical processes, which leads to the observed probability distribution functions for noncoding DNA. We argue that this difference in the shape of ...
π, γ
... T he DCJ incorporat es an array of genome rearrangement s, as shown in For t he part icular case t hat Π and Γ have t he same genes (i.e., g(Π ) = G), t he DCJ distance between Π and Γ , writ t en dD CJ (Π , Γ ), is t he mi number of DCJs required t o t ransform Π int o Γ . A closed formula fo dist ...
... T he DCJ incorporat es an array of genome rearrangement s, as shown in For t he part icular case t hat Π and Γ have t he same genes (i.e., g(Π ) = G), t he DCJ distance between Π and Γ , writ t en dD CJ (Π , Γ ), is t he mi number of DCJs required t o t ransform Π int o Γ . A closed formula fo dist ...
Document
... How do we map in humans? 1. Collect pedigrees in which the disease is present, and take blood samples of people 2. Do PCR and gel electrophoresis for 100s of SSRs spread throughout the genome 3. Do statistical analysis to determine which one SSR is the most likely to be linked to the trait locus, g ...
... How do we map in humans? 1. Collect pedigrees in which the disease is present, and take blood samples of people 2. Do PCR and gel electrophoresis for 100s of SSRs spread throughout the genome 3. Do statistical analysis to determine which one SSR is the most likely to be linked to the trait locus, g ...
الشريحة 1
... Most agarose gels are made with between 0.7% (good separation or resolution of large 5–10kb DNA fragments) and 2% (good resolution for small 0.2–1kb fragments) agarose dissolved in electrophoresis buffer. Up to 3% can be used for separating very tiny fragments but a vertical polyacrylamide gel is mo ...
... Most agarose gels are made with between 0.7% (good separation or resolution of large 5–10kb DNA fragments) and 2% (good resolution for small 0.2–1kb fragments) agarose dissolved in electrophoresis buffer. Up to 3% can be used for separating very tiny fragments but a vertical polyacrylamide gel is mo ...
BIOLOGY SUPPORT MATERIAL
... Ans: Bamboo species flower only once in their life-times generally after 50-100 years. 4- What is meant by homothallic? Ans: The term homothallic refers to bisexual or hermaphrodite condition. 5- Why are the date palms referred to as dioecious ? Ans: In date-palms, the male and female flowers are pr ...
... Ans: Bamboo species flower only once in their life-times generally after 50-100 years. 4- What is meant by homothallic? Ans: The term homothallic refers to bisexual or hermaphrodite condition. 5- Why are the date palms referred to as dioecious ? Ans: In date-palms, the male and female flowers are pr ...
MCB 371/372 - Gogarten Lab | UConn
... (frequencies) and one with final data (integer scores). Blastpgp can only use the intermediate data scoremats, and unfortunately the scoremats on the ftp side are final data scoremats. We are in the process of trying to make this easier, perhaps by placing the intermediate scoremats on the ftp site ...
... (frequencies) and one with final data (integer scores). Blastpgp can only use the intermediate data scoremats, and unfortunately the scoremats on the ftp side are final data scoremats. We are in the process of trying to make this easier, perhaps by placing the intermediate scoremats on the ftp site ...
Introduction to Molecular Systematics
... • Many models have been proposed • Elements are the rate of substitution of one base for another, per site • Rates are instantaneous (probability of change in a short period of time) • Rates may be allowed to vary among sites ...
... • Many models have been proposed • Elements are the rate of substitution of one base for another, per site • Rates are instantaneous (probability of change in a short period of time) • Rates may be allowed to vary among sites ...
Molecular Cloning and Nucleotide Sequence of the 3
... clearly read from one end to the other. However, if there were two proximal CluI sites or an EcoRI or BglII site which could not be detected by mapping with restriction endonucleases, they would be missed. To test this possibility we cleaved pYKL45 with one of ClaI, EcoRI and BglII completely, recir ...
... clearly read from one end to the other. However, if there were two proximal CluI sites or an EcoRI or BglII site which could not be detected by mapping with restriction endonucleases, they would be missed. To test this possibility we cleaved pYKL45 with one of ClaI, EcoRI and BglII completely, recir ...
Conservation of Gene Order between Horse and Human X
... markers (three microsatellites, five genes), the map incorporates polymorphic markers and genes that have already been assigned to ECAX by genetic linkage, synteny, and/or FISH techniques (Table 1 and Fig. 2). The map thus integrates loci from diverse sources and provides a basis for comparison of t ...
... markers (three microsatellites, five genes), the map incorporates polymorphic markers and genes that have already been assigned to ECAX by genetic linkage, synteny, and/or FISH techniques (Table 1 and Fig. 2). The map thus integrates loci from diverse sources and provides a basis for comparison of t ...
Cunningham Cunningham An Exploration of Bacterial
... serial dilution, the group took 10 μl from each of the sample bacteria microtubes and mixed it with 990 μl of water to create three 10^-2 dilutions. Then the group took 10 μl from each of the 10^-2 solutions and added it to 990 μl of water, creating three 10^-4 dilutions. Next, 10 μl of each of the ...
... serial dilution, the group took 10 μl from each of the sample bacteria microtubes and mixed it with 990 μl of water to create three 10^-2 dilutions. Then the group took 10 μl from each of the 10^-2 solutions and added it to 990 μl of water, creating three 10^-4 dilutions. Next, 10 μl of each of the ...
COMPUTATIONAL BIOLOGY
... from entry X01714, the one that describes its bacterial homologue. The top part of the entry follows the general information keywords order: LOCUS, ACCESSION, DEFINITION and VERSION The KEYWORD line, which is supposed to list readily relevant and searchable terms, is empty for entry U90223. Unfortun ...
... from entry X01714, the one that describes its bacterial homologue. The top part of the entry follows the general information keywords order: LOCUS, ACCESSION, DEFINITION and VERSION The KEYWORD line, which is supposed to list readily relevant and searchable terms, is empty for entry U90223. Unfortun ...
Detection of Antioxidative Activity of Plant Extracts at the DNA-modified Screen-printed Electrode
... substrate markedly delay or prevent the oxidation of the substrate [1]. They are used in the food industry to delay the oxidation process [2, 3]. The most active dietary antioxidants belong to the family of phenolic and polyphenolic compounds. Phenolic acids are widely distributed in the plant kingd ...
... substrate markedly delay or prevent the oxidation of the substrate [1]. They are used in the food industry to delay the oxidation process [2, 3]. The most active dietary antioxidants belong to the family of phenolic and polyphenolic compounds. Phenolic acids are widely distributed in the plant kingd ...
AAV-mediated Gene Therapy Restores Cone Function In A Rat With
... naturally occurring m-cone opsin mutant middle-wavelength opsin cone dysfunction, MCD) rat model. Methods: Abnormal cone response phenotype male Sprague-Dawley (SD) rats (outbreed strain) were analyzed to identify the inherited trait and the causative mutation. To develop a gene therapy, two AAV vec ...
... naturally occurring m-cone opsin mutant middle-wavelength opsin cone dysfunction, MCD) rat model. Methods: Abnormal cone response phenotype male Sprague-Dawley (SD) rats (outbreed strain) were analyzed to identify the inherited trait and the causative mutation. To develop a gene therapy, two AAV vec ...
the Acetyl-Coenzyme A
... plasmid with XbaI and PstI and transformed into strain 144-3A. The resulting gene disruption mutant was called MM67 (a ura3, leu2, his4, hurl, acsl:: LEU2). Gene disruption was confirmed by Southern analysis (Southern. 1975; Sambrook et a/., 1989) of genomic DNA digested with either BamHI or EcoRI. ...
... plasmid with XbaI and PstI and transformed into strain 144-3A. The resulting gene disruption mutant was called MM67 (a ura3, leu2, his4, hurl, acsl:: LEU2). Gene disruption was confirmed by Southern analysis (Southern. 1975; Sambrook et a/., 1989) of genomic DNA digested with either BamHI or EcoRI. ...
Chapter 13
... It goes into the complex as one doublestranded molecule, and emerges as two double-stranded molecules. ...
... It goes into the complex as one doublestranded molecule, and emerges as two double-stranded molecules. ...
Expansion of tandem repeats and oligomer
... We also calculate the expected length of repetitions if the oligomers – with the same frequencies as in real sequence – were randomly placed along an arti cial sequence. We use the expected length of repetitions of oligomers as a control. By forming the dimensionless ratio between the actual value t ...
... We also calculate the expected length of repetitions if the oligomers – with the same frequencies as in real sequence – were randomly placed along an arti cial sequence. We use the expected length of repetitions of oligomers as a control. By forming the dimensionless ratio between the actual value t ...
this help page as PDF
... exons and introns and shows where discrepancies are located. It also shows the identifiers of the target sequences. In order not to make small exons vanish when very large intronic stretches are found, the scaling of introns end exons is automatically balanced to make the picture visually meaningful ...
... exons and introns and shows where discrepancies are located. It also shows the identifiers of the target sequences. In order not to make small exons vanish when very large intronic stretches are found, the scaling of introns end exons is automatically balanced to make the picture visually meaningful ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.