An interspecific plant hybrid shows novel changes in
... Alternative splicing is a fundamental aspect of the expression of many genes. However alternative splicing patterns have not been examined in multiple genes in an interspecific plant hybrid compared with its parents. Here we studied alternative splicing patterns in an interspecific Populus hybrid an ...
... Alternative splicing is a fundamental aspect of the expression of many genes. However alternative splicing patterns have not been examined in multiple genes in an interspecific plant hybrid compared with its parents. Here we studied alternative splicing patterns in an interspecific Populus hybrid an ...
7. glossory - Shodhganga
... Heterochromatin: Chromatin that stains darkly throughout the cell cycle, even in interphase. Generally thought to be late replicating and genetically inactive. Satellite DNA in regions such ...
... Heterochromatin: Chromatin that stains darkly throughout the cell cycle, even in interphase. Generally thought to be late replicating and genetically inactive. Satellite DNA in regions such ...
Positional dependence of transcriptional inhibition by DNA torsional
... became the predominant over-represented category (Supplementary Table I). Physical clustering of altered genes on accumulation of DNA helical stress To examine how transcriptome alterations between the above top2ts and TOP2 strains spread throughout the yeast chromosomes after the accumulation of DN ...
... became the predominant over-represented category (Supplementary Table I). Physical clustering of altered genes on accumulation of DNA helical stress To examine how transcriptome alterations between the above top2ts and TOP2 strains spread throughout the yeast chromosomes after the accumulation of DN ...
PDF - The Journal of General Physiology
... p3~ decayed. A total of nine different emulsions was counted; the average star size extended from 7.4 to 15.4 rays per star. The average of these determinations for the average number of p~2 atoms per phage particle, No, is 127 4- 10. This number combined with the constant a/Vo found from the inacti ...
... p3~ decayed. A total of nine different emulsions was counted; the average star size extended from 7.4 to 15.4 rays per star. The average of these determinations for the average number of p~2 atoms per phage particle, No, is 127 4- 10. This number combined with the constant a/Vo found from the inacti ...
Isolate and Purify Phage Genomic DNA
... 1. Perform a 10-plate infection and incubate for one day (allow to grow for two days only if the phage you are working with requires it). If the plates have good "web patterns" (i. e. the number of plaques is such that their area just covers the entire plate with only a "web" of lawn remaining), the ...
... 1. Perform a 10-plate infection and incubate for one day (allow to grow for two days only if the phage you are working with requires it). If the plates have good "web patterns" (i. e. the number of plaques is such that their area just covers the entire plate with only a "web" of lawn remaining), the ...
Structure and functions of lampbrush chromosomes
... (chromomeres visible as dark irregular structures and also observed in the interphase nucleus) and side loops of decondensed chromatin. In the homologous sections of the bivalent, chromatin is condensed (spirally twisted) or decondensed in the form of side loops – two per each chromosome and four at ...
... (chromomeres visible as dark irregular structures and also observed in the interphase nucleus) and side loops of decondensed chromatin. In the homologous sections of the bivalent, chromatin is condensed (spirally twisted) or decondensed in the form of side loops – two per each chromosome and four at ...
Homologous and Nonhomologous Rearrangements: Interactions
... functions the organism is able to perform, which will in turn be compared to an environmental target to determine how well-adapted this individual is. At each generation, N new individuals are created by reproducing preferentially the best individuals of the parental generation which is then complet ...
... functions the organism is able to perform, which will in turn be compared to an environmental target to determine how well-adapted this individual is. At each generation, N new individuals are created by reproducing preferentially the best individuals of the parental generation which is then complet ...
iGCSE Biology Section 5 lesson 2
... 5.12 describe the use of restriction enzymes to cut DNA at specific sites and ligase enzymes to join pieces of DNA together 5.13 describe how plasmids and viruses can act as vectors, which take up pieces of DNA, then insert this recombinant DNA into other cells 5.14 understand that large amounts of ...
... 5.12 describe the use of restriction enzymes to cut DNA at specific sites and ligase enzymes to join pieces of DNA together 5.13 describe how plasmids and viruses can act as vectors, which take up pieces of DNA, then insert this recombinant DNA into other cells 5.14 understand that large amounts of ...
Arabidopsis thaliana Arabidopsis thaliana
... Unlike animals, which use a sodium ion P-type ATPase pump to generate an electrochemical gradient across the plasma membrane, plants and fungi use a proton Ptype ATPase pump to form a large membrane potential. plant secondary transporters are typically coupled to protons rather than to sodium. -alm ...
... Unlike animals, which use a sodium ion P-type ATPase pump to generate an electrochemical gradient across the plasma membrane, plants and fungi use a proton Ptype ATPase pump to form a large membrane potential. plant secondary transporters are typically coupled to protons rather than to sodium. -alm ...
Gene Loss and Evolutionary Rates Following Whole
... 2003), as outgroups to actinopterygian fishes. Amino acid sequences were aligned using ClustalW (Thomson et al. 1994), followed by manual adjustments if needed. We defined groups of paralogs as 2 or 3 Tetraodon genes that have the same human best hit from Swissprot, using Blast with the settings use ...
... 2003), as outgroups to actinopterygian fishes. Amino acid sequences were aligned using ClustalW (Thomson et al. 1994), followed by manual adjustments if needed. We defined groups of paralogs as 2 or 3 Tetraodon genes that have the same human best hit from Swissprot, using Blast with the settings use ...
manual K. lactis Protein Expression Kit E1000S
... promoter (PLAC4-PBI) that has been engineered to lack background E. coli transcriptional activity (2). Therefore, genes encoding products toxic to E. coli can be cloned into pKLAC2 in E. coli prior to their introduction into yeast cells. To achieve expression in yeast, pKLAC2 containing a cloned gen ...
... promoter (PLAC4-PBI) that has been engineered to lack background E. coli transcriptional activity (2). Therefore, genes encoding products toxic to E. coli can be cloned into pKLAC2 in E. coli prior to their introduction into yeast cells. To achieve expression in yeast, pKLAC2 containing a cloned gen ...
Interaction of DNA with ribosomes in cell-free protein
... illumination at 30 °C. The single steps of the prepara tion of ribosomes and supernatant enzymes have been previously described *. After disruption of the cells with glass beads, the homogenate was precentrifuged 30 min at 30 000 g. From this supernatant, the ribo somes were pelleted after 90 min ...
... illumination at 30 °C. The single steps of the prepara tion of ribosomes and supernatant enzymes have been previously described *. After disruption of the cells with glass beads, the homogenate was precentrifuged 30 min at 30 000 g. From this supernatant, the ribo somes were pelleted after 90 min ...
SGD: Saccharomyces Genome Database.
... (GenBank, EMBL, DDBJ, SwissProt and PIR). It then assembles it into datasets (described below) that make the sequence information more useful to molecular biologists. These datasets are available from SGD through the World Wide Web and Anonymous FTP. The genomic sequence. In April 1996, the complete ...
... (GenBank, EMBL, DDBJ, SwissProt and PIR). It then assembles it into datasets (described below) that make the sequence information more useful to molecular biologists. These datasets are available from SGD through the World Wide Web and Anonymous FTP. The genomic sequence. In April 1996, the complete ...
Comprehension Question
... Strengths – Fruit flies have proven to be an excellent model system for studying aspects of biology that they share with humans. Fruit flies are simpler in structure and physiology than humans and have a much simpler genome. They are small and easy to raise, they have a short generation time, and th ...
... Strengths – Fruit flies have proven to be an excellent model system for studying aspects of biology that they share with humans. Fruit flies are simpler in structure and physiology than humans and have a much simpler genome. They are small and easy to raise, they have a short generation time, and th ...
Carbapenemase and virulence factors of
... However, Escherichia coli represented 73% of the OXA-48-producing Enterobacteriaceae collected in 2012 at this hospital. During the faecal carriage study performed in non-hospitalized patients, E. coli was the only species producing OXA-48. The blaOXA-48 gene was mainly found within Tn1999.2-type tr ...
... However, Escherichia coli represented 73% of the OXA-48-producing Enterobacteriaceae collected in 2012 at this hospital. During the faecal carriage study performed in non-hospitalized patients, E. coli was the only species producing OXA-48. The blaOXA-48 gene was mainly found within Tn1999.2-type tr ...
unit II - SP College
... Satellite DNA adopts higher-order three-dimensional structures in eukaryotic organisms. This was demonstrated in the land crab Gecarcinuslateralis, whose DNA contains 3% of a GC-rich sequence consisting of repeats of a ~2100 base pair (bp) sequence called RU. The RU was arranged in long tandem array ...
... Satellite DNA adopts higher-order three-dimensional structures in eukaryotic organisms. This was demonstrated in the land crab Gecarcinuslateralis, whose DNA contains 3% of a GC-rich sequence consisting of repeats of a ~2100 base pair (bp) sequence called RU. The RU was arranged in long tandem array ...
Chase, B. A., and Baker, B. S.
... Sex-type in Dros@hila mlanogaster is controlled by a hierarchically acting setof regulatory genes. At the terminusof this hierarchy lie those regulatory genes responsible for implementing sexual differentiation: genes that control the activity of target loci whose products give rise to sexually dimo ...
... Sex-type in Dros@hila mlanogaster is controlled by a hierarchically acting setof regulatory genes. At the terminusof this hierarchy lie those regulatory genes responsible for implementing sexual differentiation: genes that control the activity of target loci whose products give rise to sexually dimo ...
MEDICAL BIOLOGY AND GENERAL GENETICS
... and properties of cell organelles: a tissue specimen is fragmentized to destroy cell membranes, then placed into the centrifuge, where it is divided into separate fractions. 5. The method of autography is used for studying the dynamic of metabolic processes in cell compnents. It is based on introduc ...
... and properties of cell organelles: a tissue specimen is fragmentized to destroy cell membranes, then placed into the centrifuge, where it is divided into separate fractions. 5. The method of autography is used for studying the dynamic of metabolic processes in cell compnents. It is based on introduc ...
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... and the pNL4-‐3 wt plasmid were then digested with both AgeI and EcoRI (New England Biolabs). In order to confirm the insertion of the BstEII restriction site, the PCR pNL4-‐3.AgeIEcoRI was ...
... and the pNL4-‐3 wt plasmid were then digested with both AgeI and EcoRI (New England Biolabs). In order to confirm the insertion of the BstEII restriction site, the PCR pNL4-‐3.AgeIEcoRI was ...
nucleicacidchemistry
... sequence appears in the DNA, the enzyme cleaves it When the corresponding methylated sequence appears, it doesn’t get cleaved and remains available for replication The restriction endonucleases only bind to palindromic sequences ...
... sequence appears in the DNA, the enzyme cleaves it When the corresponding methylated sequence appears, it doesn’t get cleaved and remains available for replication The restriction endonucleases only bind to palindromic sequences ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.