PPT - International Wheat Genome Sequencing Consortium
... • Annotation (RNASeq, FLcDNA, grass genomes): 124,201 allocated to chromosomes ...
... • Annotation (RNASeq, FLcDNA, grass genomes): 124,201 allocated to chromosomes ...
Table II Transformation of various derived strains OSU Strain Outcrossed with
... 1984 Neurospora Newsl. 31: 32). Each E. coli clone of the library contains recombinant DNA molecules i.e. fragments of Neursopora DNA inserted into the BamHI site of the cosmid vector. DNA was prepared from all 5000 clones of the library (a recombinant cosmid-pool) and it was purified further on a C ...
... 1984 Neurospora Newsl. 31: 32). Each E. coli clone of the library contains recombinant DNA molecules i.e. fragments of Neursopora DNA inserted into the BamHI site of the cosmid vector. DNA was prepared from all 5000 clones of the library (a recombinant cosmid-pool) and it was purified further on a C ...
1 Genetics (BIL-250) Review Questions #1 (2
... (3-1) Draw a DNA replication fork and identify and label the locations of the following major components: (1) 5’ and 3’ ends of each strand, (2) leading strand, (3) lagging strand, (4) single-stranded binding proteins, (5) DNA polymerase, (6)Okazaki fragments, (7) RNA primer, (8) DNA helicase, (9) D ...
... (3-1) Draw a DNA replication fork and identify and label the locations of the following major components: (1) 5’ and 3’ ends of each strand, (2) leading strand, (3) lagging strand, (4) single-stranded binding proteins, (5) DNA polymerase, (6)Okazaki fragments, (7) RNA primer, (8) DNA helicase, (9) D ...
Genetics Exam 3
... cell types. ________________________________ A chromosomal mutation in which there is a change in position of chromosome segments to a different location in the genome. ________________________________ A gene present in only one dose. ________________________________ An enzyme that introduces or eli ...
... cell types. ________________________________ A chromosomal mutation in which there is a change in position of chromosome segments to a different location in the genome. ________________________________ A gene present in only one dose. ________________________________ An enzyme that introduces or eli ...
DNA Sequencing
... 13.3 Cloned Libraries Libraries are resources for gene studies To make a human genomic library using bacterial plasmids ~8 million plasmid clones would be needed (plasmids generally can carry DNA fragments of about 1700 base pairs long) ...
... 13.3 Cloned Libraries Libraries are resources for gene studies To make a human genomic library using bacterial plasmids ~8 million plasmid clones would be needed (plasmids generally can carry DNA fragments of about 1700 base pairs long) ...
DISCOVERY OF DNAhandout
... 3. Treated with deoxyribonuclease, which eliminates all DNA The result: ...
... 3. Treated with deoxyribonuclease, which eliminates all DNA The result: ...
Biology Vocabulary 8, test on Thursday, 1/19/17
... permanent change in a cell's DNA, ranging from changes in a single base pair to deletions of large sections of chromosomes cell division in which the sister chromatids do not separate correctly, resulting in gametes with an abnormal number of chromosomes characteristic, such as eye color or skin col ...
... permanent change in a cell's DNA, ranging from changes in a single base pair to deletions of large sections of chromosomes cell division in which the sister chromatids do not separate correctly, resulting in gametes with an abnormal number of chromosomes characteristic, such as eye color or skin col ...
Learner outcomes File
... separated according to their size. - State that gel electrophoresis of DNA is used in DNA profiling. - Describe the application of DNA profiling to determine paternity and also in forensic investigations. - Analyze DNA profiles to draw conclusions about paternity or forensic investigations. - Outlin ...
... separated according to their size. - State that gel electrophoresis of DNA is used in DNA profiling. - Describe the application of DNA profiling to determine paternity and also in forensic investigations. - Analyze DNA profiles to draw conclusions about paternity or forensic investigations. - Outlin ...
Chapter 14
... • Heat is applied to denature the double-stranded DNA fragments and then cooled to allow for the primer to bind to a single strand of DNA • DNA polymerase synthesizes a complementary strand until a chemical tag is incorporated because of the relatively low concentration of chemical tags compared w ...
... • Heat is applied to denature the double-stranded DNA fragments and then cooled to allow for the primer to bind to a single strand of DNA • DNA polymerase synthesizes a complementary strand until a chemical tag is incorporated because of the relatively low concentration of chemical tags compared w ...
Epigenet-web
... • All cells in the organism carry the same genetic material, however each cell type expresses different genes. ...
... • All cells in the organism carry the same genetic material, however each cell type expresses different genes. ...
DNA (deoxyribonucleic acid ) **Long molecule made up of units
... DNA molecules are very long and must be folded into a space only one one-thousandth of its length. They are found in the nucleus of the cell. Example: ** The chromosome of a bacterial cell in the human colon contains 4,639,221 base pairs. **A human cell contains almost 1000 times as many base pairs ...
... DNA molecules are very long and must be folded into a space only one one-thousandth of its length. They are found in the nucleus of the cell. Example: ** The chromosome of a bacterial cell in the human colon contains 4,639,221 base pairs. **A human cell contains almost 1000 times as many base pairs ...
Document
... 13. Several forms of RNA or ______________________ help change DNA code into proteins. 14. Because it is so similar to ______________________, RNA can serve as a temporary copy of a DNA sequence. 15. The “factory” that assembles proteins is known as a(n) ______________________. 16. A mirror-like cop ...
... 13. Several forms of RNA or ______________________ help change DNA code into proteins. 14. Because it is so similar to ______________________, RNA can serve as a temporary copy of a DNA sequence. 15. The “factory” that assembles proteins is known as a(n) ______________________. 16. A mirror-like cop ...
Cell Cycle SG
... 14. The bases are always added from the ____________ direction to form the new DNA strands. 15. What makes a DNA molecule antiparallel? List all the differences between the leading & lagging strands. Strand How It is Built 16. leading strand 17. lagging strand 18. Label the following drawing of the ...
... 14. The bases are always added from the ____________ direction to form the new DNA strands. 15. What makes a DNA molecule antiparallel? List all the differences between the leading & lagging strands. Strand How It is Built 16. leading strand 17. lagging strand 18. Label the following drawing of the ...
deoxyribonucleic acid contained in the chromosomes humans have
... DNA Replication DNA can make a copy of it itself BECAUSE of the way the bases pair up 1) the DNA strand will 'unzip' as the chemical bonds are broken between each of the nitrogen bases 2) 'loose' nitrogen bases of the correct type will adhere to the free one 3) the ends of the newly attache ...
... DNA Replication DNA can make a copy of it itself BECAUSE of the way the bases pair up 1) the DNA strand will 'unzip' as the chemical bonds are broken between each of the nitrogen bases 2) 'loose' nitrogen bases of the correct type will adhere to the free one 3) the ends of the newly attache ...
DIR RD 4C-2
... 13. Several forms of RNA or ______________________ help change DNA code into proteins. 14. Because it is so similar to ______________________, RNA can serve as a temporary copy of a DNA sequence. 15. The “factory” that assembles proteins is known as a(n) ______________________. 16. A mirror-like cop ...
... 13. Several forms of RNA or ______________________ help change DNA code into proteins. 14. Because it is so similar to ______________________, RNA can serve as a temporary copy of a DNA sequence. 15. The “factory” that assembles proteins is known as a(n) ______________________. 16. A mirror-like cop ...
Cloning Power Point
... multiple copies of that gene using bacterial plasmids, selfreplicating extra-chromosomal circular DNA molecules, that are distinctly different from the normal bacterial genome. Genes and other chromosomes are copied to make enough samples for further study. In order to clone a gene, a fragment of DN ...
... multiple copies of that gene using bacterial plasmids, selfreplicating extra-chromosomal circular DNA molecules, that are distinctly different from the normal bacterial genome. Genes and other chromosomes are copied to make enough samples for further study. In order to clone a gene, a fragment of DN ...
GENETICS
... • Genes (“ DNA words”) are parts of the DNA molecule made of groups base pairs triplets (“3 DNA letters = 1 word or gene) • Each gene instructs the body to make 1 protein • These proteins include enzymes, structural proteins, hormones etc. Together these proteins allow an organism to live, grow and ...
... • Genes (“ DNA words”) are parts of the DNA molecule made of groups base pairs triplets (“3 DNA letters = 1 word or gene) • Each gene instructs the body to make 1 protein • These proteins include enzymes, structural proteins, hormones etc. Together these proteins allow an organism to live, grow and ...
Chap 3
... for proteins 3. The human genome has significantly more duplicated segments within it that other mammalian genomes do. These sections may be the source of new primatespecific genes. 4. At the time when the draft sequence was published less than 7% of protein families appeared to be vertebrate-specif ...
... for proteins 3. The human genome has significantly more duplicated segments within it that other mammalian genomes do. These sections may be the source of new primatespecific genes. 4. At the time when the draft sequence was published less than 7% of protein families appeared to be vertebrate-specif ...
GENETICS SOL REVIEW – 2015 PART II Name ____________________________
... Process of cells reduction division of chromosomes; produces gametes Reproductive cell; eggs and sperm Specific characteristics Traits determined by genes located on the X chromosome In the first meiotic division chromosomes exchange segments of their DNA The likelihood, or chance, something will ha ...
... Process of cells reduction division of chromosomes; produces gametes Reproductive cell; eggs and sperm Specific characteristics Traits determined by genes located on the X chromosome In the first meiotic division chromosomes exchange segments of their DNA The likelihood, or chance, something will ha ...
Document
... Genetic Engineering - the deliberate modification of an organisms genome. The methods used to accomplish this are know as recombinant DNA technology Recombinant DNA - DNA with a new sequence formed by joining fragments from different sources Restriction enzymes - bacterial enzymes (endonucleases) th ...
... Genetic Engineering - the deliberate modification of an organisms genome. The methods used to accomplish this are know as recombinant DNA technology Recombinant DNA - DNA with a new sequence formed by joining fragments from different sources Restriction enzymes - bacterial enzymes (endonucleases) th ...
Document
... They used bacterial plasmids which are small circular replicating fragments of DNA They also used enzymes that cut DNA into specific fragments. These enzymes are called restriction endonucleases (enzymes ...
... They used bacterial plasmids which are small circular replicating fragments of DNA They also used enzymes that cut DNA into specific fragments. These enzymes are called restriction endonucleases (enzymes ...
Genetic Engineering Notes
... o The most common vector is a ___________that has been genetically altered to carry normal human DNA. o Ex: To reverse disease caused by genetic damage, researchers isolate normal DNA and package it into a vector, a molecular delivery truck usually made from a disabled virus. Doctors then infect a t ...
... o The most common vector is a ___________that has been genetically altered to carry normal human DNA. o Ex: To reverse disease caused by genetic damage, researchers isolate normal DNA and package it into a vector, a molecular delivery truck usually made from a disabled virus. Doctors then infect a t ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.