PowerPoint
... Gene coding for specific functions needs to be located on the DNA before recombinant DNA technology is applied. • 1. A complete complement of genetic material in an organism is the genome. • 2. Locating and recording the site of specific genes within the chromosomes is gene mapping. Selected port ...
... Gene coding for specific functions needs to be located on the DNA before recombinant DNA technology is applied. • 1. A complete complement of genetic material in an organism is the genome. • 2. Locating and recording the site of specific genes within the chromosomes is gene mapping. Selected port ...
Chapter 13 Biotechnology 2013
... Takes less time than use of living cells Requires less amount of desired DNA initially Used for cloning rare DNA & ID small amounts of infectious DNA (AIDS) ...
... Takes less time than use of living cells Requires less amount of desired DNA initially Used for cloning rare DNA & ID small amounts of infectious DNA (AIDS) ...
1 - life.illinois.edu
... c. genes encoding proteins allowing for conjugation between different bacteria. d. genes encoding proteins from humans. 32. A cDNA clone would contain only the __________ of a protein-coding gene. a. exons b. introns c. promoter d. spacer 33. Short tandem repeats (microsatellites) in our genome are ...
... c. genes encoding proteins allowing for conjugation between different bacteria. d. genes encoding proteins from humans. 32. A cDNA clone would contain only the __________ of a protein-coding gene. a. exons b. introns c. promoter d. spacer 33. Short tandem repeats (microsatellites) in our genome are ...
Chapter 13 DNA Technology
... made even when the original sample does not supply does not supply enough. This helps a lot when working a crime scene!! Materials List for PCR – a DNA sample, a supply of the 4 DNA nucleotides, DNA polymerase, and primers (artificially made single stranded piece of DNA needed to start replication). ...
... made even when the original sample does not supply does not supply enough. This helps a lot when working a crime scene!! Materials List for PCR – a DNA sample, a supply of the 4 DNA nucleotides, DNA polymerase, and primers (artificially made single stranded piece of DNA needed to start replication). ...
MCB5472_Lecture_2_Feb-3-14
... read assemblers only use read pairing information AFTER contig assembly during scaffolding – This is starting to change as algorithms ...
... read assemblers only use read pairing information AFTER contig assembly during scaffolding – This is starting to change as algorithms ...
6.2 Recombinant DNA Technology
... Fragments of human DNA and plasmid mixed together and join Plasmids enter the bacterial cells, copy themselves, carry recombinant DNA into bacteria Bacteria express gene, synthesize the human protein, can be used for treatments, vaccines, or other purposes ...
... Fragments of human DNA and plasmid mixed together and join Plasmids enter the bacterial cells, copy themselves, carry recombinant DNA into bacteria Bacteria express gene, synthesize the human protein, can be used for treatments, vaccines, or other purposes ...
The human genome
... almost illiterate. Since he was unable to read as a child, he concentrated his curiosity on the plant and animal life on the family estate. One day, says a story, he saw a gardener planting beans. The boy asked why the man was planting sticks of wood and was told they would grow up to be beans. The ...
... almost illiterate. Since he was unable to read as a child, he concentrated his curiosity on the plant and animal life on the family estate. One day, says a story, he saw a gardener planting beans. The boy asked why the man was planting sticks of wood and was told they would grow up to be beans. The ...
Unit Study Guide
... 3. What is the monomer of this macromolecule and what are the three parts that make up this macromolecule? 4. What do the bases attach to at the backbone? What type of bonds hold the bases together in the middle? 5. What is Chargaff’s rule? 6. What term is used to describe the shape of DNA? 7. Speci ...
... 3. What is the monomer of this macromolecule and what are the three parts that make up this macromolecule? 4. What do the bases attach to at the backbone? What type of bonds hold the bases together in the middle? 5. What is Chargaff’s rule? 6. What term is used to describe the shape of DNA? 7. Speci ...
Chapter 12 - Biotechnology
... Recombinant DNA Technology Restriction enzymes • Restriction enzymes were discovered in bacteria. Bacteria use them as a defense mechanism to cut up the DNA of viruses or other bacteria. • Hundreds of different restriction enzymes have been isolated. Each one cuts DNA at a specific base sequence. F ...
... Recombinant DNA Technology Restriction enzymes • Restriction enzymes were discovered in bacteria. Bacteria use them as a defense mechanism to cut up the DNA of viruses or other bacteria. • Hundreds of different restriction enzymes have been isolated. Each one cuts DNA at a specific base sequence. F ...
命題標頭紙 - 慈濟大學醫學資訊學系所
... 9. A BLAST search yields several hits. Among them, subject A with 95% identity and E-value = 0.1, and subject B with 65% identity and E-value = e-32. Which one is a better hit? Why? (5%) 10. Palindromes are DNA sequences in which the reverse complement is identical to the positive strand, such as GT ...
... 9. A BLAST search yields several hits. Among them, subject A with 95% identity and E-value = 0.1, and subject B with 65% identity and E-value = e-32. Which one is a better hit? Why? (5%) 10. Palindromes are DNA sequences in which the reverse complement is identical to the positive strand, such as GT ...
Chapter 12 - Biotechnology
... Recombinant DNA Technology Restriction enzymes • Restriction enzymes were discovered in bacteria. Bacteria use them as a defense mechanism to cut up the DNA of viruses or other bacteria. • Hundreds of different restriction enzymes have been isolated. Each one cuts DNA at a specific base sequence. F ...
... Recombinant DNA Technology Restriction enzymes • Restriction enzymes were discovered in bacteria. Bacteria use them as a defense mechanism to cut up the DNA of viruses or other bacteria. • Hundreds of different restriction enzymes have been isolated. Each one cuts DNA at a specific base sequence. F ...
Genetics 310 Practice exam III-1
... 1. What are the two types of molecules found in eukaryotic chromosomes? 2. True or False? ____ Man has more DNA per genome than all other organisms. ____ The number of chromosomes is a direct reflection of the amount of DNA/genome in a species. ____ All of the DNA in a eukaryote is unique sequence D ...
... 1. What are the two types of molecules found in eukaryotic chromosomes? 2. True or False? ____ Man has more DNA per genome than all other organisms. ____ The number of chromosomes is a direct reflection of the amount of DNA/genome in a species. ____ All of the DNA in a eukaryote is unique sequence D ...
Chapter 13 – Genetic Engineering
... producing a successful mutant. – Ex – bacteria that can digest oil have been produced this way ...
... producing a successful mutant. – Ex – bacteria that can digest oil have been produced this way ...
Chapter 13 – Genetic Engineering
... producing a successful mutant. – Ex – bacteria that can digest oil have been produced this way ...
... producing a successful mutant. – Ex – bacteria that can digest oil have been produced this way ...
Agrobacterium
... Sometimes, this fragment will be incorporated into the host (human) genome. Problems: Viruses are scary and germy! Immune system response can be serious. The fragment could happen to land in the middle of a functional gene. More feasible: implant stem cells that have undergone genetic engineering. ...
... Sometimes, this fragment will be incorporated into the host (human) genome. Problems: Viruses are scary and germy! Immune system response can be serious. The fragment could happen to land in the middle of a functional gene. More feasible: implant stem cells that have undergone genetic engineering. ...
Chapter 13 – Genetic Engineering
... – Ex – bananas, citrus fruit, strawberries, many ornamental flowers Diploid corn Tetraploid corn ...
... – Ex – bananas, citrus fruit, strawberries, many ornamental flowers Diploid corn Tetraploid corn ...
18.3 GMO, GMF & Cloning
... Examples 1) Corn (GMF) → over ½ of the corn produced today contains recombinant DNA → for example, herbicide-resistant corn can be sprayed with herbicide and not be harmed. 2) insulin-producing bacteria (GMO) → in the past, insulin was only available from animal sources. Now, it can be produced by ...
... Examples 1) Corn (GMF) → over ½ of the corn produced today contains recombinant DNA → for example, herbicide-resistant corn can be sprayed with herbicide and not be harmed. 2) insulin-producing bacteria (GMO) → in the past, insulin was only available from animal sources. Now, it can be produced by ...
L 17 _PCR
... Usually we want to sequence a segment of DNA longer than 600 bp. We have three choices: a. “subclones” of different fragments and sequence the ends of each, using vector primers b. sequence once for 600 bp, then design and synthesize a new primer to the end of the that sequence; repeat until the end ...
... Usually we want to sequence a segment of DNA longer than 600 bp. We have three choices: a. “subclones” of different fragments and sequence the ends of each, using vector primers b. sequence once for 600 bp, then design and synthesize a new primer to the end of the that sequence; repeat until the end ...
Unit 6 Part 2 Notes Jan 16 2012
... • It is highly unlikely that an individual would have the same number of repeats as another individual at the most common13 STR sites used by criminal justice to distinguish individuals. ...
... • It is highly unlikely that an individual would have the same number of repeats as another individual at the most common13 STR sites used by criminal justice to distinguish individuals. ...
Agrobacterium tumefaciens
... • Average ORF 317 amino acids • Average gene size 1000 bp • Very compact: average distance between genes 118bp Contour length of genome: 1.7 mm It can accept foreign DNA derived from any organism Some genes are arranged in the plasmid ...
... • Average ORF 317 amino acids • Average gene size 1000 bp • Very compact: average distance between genes 118bp Contour length of genome: 1.7 mm It can accept foreign DNA derived from any organism Some genes are arranged in the plasmid ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.