Genetics of Viruses and Bacteria
... assembled inside a viral capsid Crossover occurs between new transduced DNA and new host DNA ...
... assembled inside a viral capsid Crossover occurs between new transduced DNA and new host DNA ...
RESTRICTION ENDONUCLEASES
... early 1970s and the recognition that these enzymes act as “molecular scissors”, always cutting DNA at specific locations (base sequences), was the key discovery which allowed the cloning of DNA to become a reality (Turner et al., 1997). Restriction enzymes are proteins that bind to a specific DNA se ...
... early 1970s and the recognition that these enzymes act as “molecular scissors”, always cutting DNA at specific locations (base sequences), was the key discovery which allowed the cloning of DNA to become a reality (Turner et al., 1997). Restriction enzymes are proteins that bind to a specific DNA se ...
Tandem repeats - Trimble County Schools
... Short Tandem Repeats (STR) • Latest method of DNA analysis • Locations on the chromosome that contain short sequence elements that repeat • Less than 400 base pairs • Shorter than RFLP • Less susceptible to degradation • Can be recovered from bodies or stains that have been subject to decomposition ...
... Short Tandem Repeats (STR) • Latest method of DNA analysis • Locations on the chromosome that contain short sequence elements that repeat • Less than 400 base pairs • Shorter than RFLP • Less susceptible to degradation • Can be recovered from bodies or stains that have been subject to decomposition ...
Cloning and Sequencing
... It requires ligating (splicing) the PCR product into a cloning vector – often a plasmid DNA The recombinant DNA of the ligation product can now be put into a cell to propagate (replicated) ...
... It requires ligating (splicing) the PCR product into a cloning vector – often a plasmid DNA The recombinant DNA of the ligation product can now be put into a cell to propagate (replicated) ...
Exempt Dealing Application Form - Macquarie University
... 4. Describe the host organism or tissue to be used – include the genus, species and strain where applicable. If not a commonly used laboratory strain, include the name of the strain from which it is derived. ...
... 4. Describe the host organism or tissue to be used – include the genus, species and strain where applicable. If not a commonly used laboratory strain, include the name of the strain from which it is derived. ...
WEBQUEST – DNA and Protein Synthesis
... 7. a. The RNA polymerase makes a copy of the LUC gene in what form? _____________ b. Once transcription is complete, where does the mRNA go next? _________________ 8. What is the cell’s protein-making machine? _________________ 9. What is the being make through the string of amino acids? ___________ ...
... 7. a. The RNA polymerase makes a copy of the LUC gene in what form? _____________ b. Once transcription is complete, where does the mRNA go next? _________________ 8. What is the cell’s protein-making machine? _________________ 9. What is the being make through the string of amino acids? ___________ ...
13.2 Notes - Trimble County Schools
... Short Tandem Repeats (STR) • Latest method of DNA analysis • Locations on the chromosome that contain short sequence elements that repeat • Less than 400 base pairs • Shorter than RFLP • Less susceptible to degradation • Can be recovered from bodies or stains that have been subject to decompositio ...
... Short Tandem Repeats (STR) • Latest method of DNA analysis • Locations on the chromosome that contain short sequence elements that repeat • Less than 400 base pairs • Shorter than RFLP • Less susceptible to degradation • Can be recovered from bodies or stains that have been subject to decompositio ...
DNA replication is molecular mechanism of
... Eukaryotes, however, have multiple separate linear chromosomes. DNA replication begins at multiple sites along the length of each chromosome. What do we call the position(s) on the chromosome(s) where DNA replication begin(s)? ...
... Eukaryotes, however, have multiple separate linear chromosomes. DNA replication begins at multiple sites along the length of each chromosome. What do we call the position(s) on the chromosome(s) where DNA replication begin(s)? ...
PCR-assay of intragenic DNA lesions induced by ionizing radiation
... 3. Project Summary: 3.1. Goal of Project: The goal of the Project is to detect the nature and location of DNA alterations induced by γ-rays and neutrons at the regulatory and coding parts of yellow gene Drosophila melanogaster. 3.2. Background and Topicality of Project: A large body of experimental ...
... 3. Project Summary: 3.1. Goal of Project: The goal of the Project is to detect the nature and location of DNA alterations induced by γ-rays and neutrons at the regulatory and coding parts of yellow gene Drosophila melanogaster. 3.2. Background and Topicality of Project: A large body of experimental ...
Chapter 13
... 23. Describe Hfr strains and interrupted mating technique in constructing the E. coli minute map 24. Explain why recipient cells of an Hfr mating remain F-. 25. Examine homologous recombination in a recipient, exconjugant cell 26. Analyze the creation of knockout mice via homologous recombination an ...
... 23. Describe Hfr strains and interrupted mating technique in constructing the E. coli minute map 24. Explain why recipient cells of an Hfr mating remain F-. 25. Examine homologous recombination in a recipient, exconjugant cell 26. Analyze the creation of knockout mice via homologous recombination an ...
Genome Instability and Repair
... • Local genome instability • Genomic rearrangements, new exons, etc. (Cordaux & Batzer (2010) Nat. Rev. Genet. 10, 691-703) ...
... • Local genome instability • Genomic rearrangements, new exons, etc. (Cordaux & Batzer (2010) Nat. Rev. Genet. 10, 691-703) ...
Biology Assessment #3:
... 2. Compare and contrast DNA and RNA (location, function, size/structure/shape, nitrogen bases, etc.) 3. Explain how protein synthesis occurs. Explain the steps of transcription/translation. What is the role of different the types of RNA and of DNA in protein synthesis? 4. How are amino acids coded f ...
... 2. Compare and contrast DNA and RNA (location, function, size/structure/shape, nitrogen bases, etc.) 3. Explain how protein synthesis occurs. Explain the steps of transcription/translation. What is the role of different the types of RNA and of DNA in protein synthesis? 4. How are amino acids coded f ...
Selection Coevolution
... Red Queen situation: to remain in one place (survive without being vulnerable to pathogens), you have to keep evolving new defenses. In this case there is frequency-dependent selection, where the common phenotype has a reduced fitness (pathogens will easily infect individuals who all have the same i ...
... Red Queen situation: to remain in one place (survive without being vulnerable to pathogens), you have to keep evolving new defenses. In this case there is frequency-dependent selection, where the common phenotype has a reduced fitness (pathogens will easily infect individuals who all have the same i ...
Multi-copy suppressor screen
... might show novel phenotypes caused by the mutation such as temperature sensitivity. Calculation exercise: Assume you have 1 ug of plasmid DNA. What is the molecular weight of pRS316? How many plasmid molecules are present in 1 ug of DNA? ...
... might show novel phenotypes caused by the mutation such as temperature sensitivity. Calculation exercise: Assume you have 1 ug of plasmid DNA. What is the molecular weight of pRS316? How many plasmid molecules are present in 1 ug of DNA? ...
PCR
... cloning and expressing eukaryotic genes. • Yeast cells, single-celled fungi, are as easy to grow as bacteria and have plasmids, rare for eukaryotes. • Scientists have constructed yeast artificial chromosomes (YACs) - an origin site for replication, a centromere, and two telomeres with foreign DNA. • ...
... cloning and expressing eukaryotic genes. • Yeast cells, single-celled fungi, are as easy to grow as bacteria and have plasmids, rare for eukaryotes. • Scientists have constructed yeast artificial chromosomes (YACs) - an origin site for replication, a centromere, and two telomeres with foreign DNA. • ...
Mutations
... They cause disease because changes in the genome's instructions alter the functions of important proteins that are needed for health. For example, diabetes, cancer, heart disease, and hemophilia all result from mutations that cause harmful effects. ...
... They cause disease because changes in the genome's instructions alter the functions of important proteins that are needed for health. For example, diabetes, cancer, heart disease, and hemophilia all result from mutations that cause harmful effects. ...
Aliens? - Johns Hopkins Bloomberg School of Public Health
... – RepeatMasker (Smit & Jurka) – Problem: each element is at least in part unique, and RepeatMasker will mask that too ...
... – RepeatMasker (Smit & Jurka) – Problem: each element is at least in part unique, and RepeatMasker will mask that too ...
Biotechnology - BHSBiology-Cox
... genetically modified – Organisms is expressing genes that did not occur naturally – A new gene was inserted into the genome of an organism ...
... genetically modified – Organisms is expressing genes that did not occur naturally – A new gene was inserted into the genome of an organism ...
Status and plans, human vs. mouse alignments
... • Posterior probability that a site is among the most highly conserved sites • Allows for variation in rates along lineages c is “conserved” (constrained) n is “nonconserved” (aligns but is not clearly subject to purifying selection) Siepel et al. (2005) Genome Research 15:1034-1050 ...
... • Posterior probability that a site is among the most highly conserved sites • Allows for variation in rates along lineages c is “conserved” (constrained) n is “nonconserved” (aligns but is not clearly subject to purifying selection) Siepel et al. (2005) Genome Research 15:1034-1050 ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.