2010 SEC Definition-style Questions
... (A nucleus having) Two sets of chromosomes (or two copies of each chromosome) ...
... (A nucleus having) Two sets of chromosomes (or two copies of each chromosome) ...
CHAPTER 17 RECOMBINANT DNA AND BIOTECHNOLOGY
... b. The restriction enzyme is used to cut DNA at specific points during production of rDNA. c. It is called a restriction enzyme because it restricts growth of viruses but it acts a molecular scissors to cleave any piece of DNA at a specific site. 7. Restriction enzymes cleave vector (plasmid) and fo ...
... b. The restriction enzyme is used to cut DNA at specific points during production of rDNA. c. It is called a restriction enzyme because it restricts growth of viruses but it acts a molecular scissors to cleave any piece of DNA at a specific site. 7. Restriction enzymes cleave vector (plasmid) and fo ...
I.
... (D) choice of alternative poly(A) sites !2.A locally folded region of tertiary structure interconnected by the polypeptide strand into the ...
... (D) choice of alternative poly(A) sites !2.A locally folded region of tertiary structure interconnected by the polypeptide strand into the ...
Document
... Self-ligation of vector Ligation of vector to primer-dimers Ligation of multiple inserts Self-ligation of inserts Ligation of one insert into vector ...
... Self-ligation of vector Ligation of vector to primer-dimers Ligation of multiple inserts Self-ligation of inserts Ligation of one insert into vector ...
Cloning Restriction Fragments of Cellular DNA
... – plasmids containing drug resistance gene (up to 10 kb) • many commercially available plasmids ...
... – plasmids containing drug resistance gene (up to 10 kb) • many commercially available plasmids ...
Genetics
... • DNA is mutable • A variation in DNA sequence at a locus is called an allele – Diploid organisms contain 2 alleles of each locus (gene) • Alleles can be identical – homozygous • Alleles can be different – heterozygous • If only one allele is present – hemizygous – Case in males for genes on X and Y ...
... • DNA is mutable • A variation in DNA sequence at a locus is called an allele – Diploid organisms contain 2 alleles of each locus (gene) • Alleles can be identical – homozygous • Alleles can be different – heterozygous • If only one allele is present – hemizygous – Case in males for genes on X and Y ...
Genome_annotation
... •pilot phase focused on 30 Mb (~ 1%) of the genome •international consortium of computational and laboratory-based scientists working to develop and apply high-throughput approaches for detecting all sequence elements that confer biological function •now in its second phase, extending study to entir ...
... •pilot phase focused on 30 Mb (~ 1%) of the genome •international consortium of computational and laboratory-based scientists working to develop and apply high-throughput approaches for detecting all sequence elements that confer biological function •now in its second phase, extending study to entir ...
slides
... • DNA microarrays – a collection of microscopic DNA spots representing single genes. • Commonly used to monitor expression levels of thousands of genes at once. ...
... • DNA microarrays – a collection of microscopic DNA spots representing single genes. • Commonly used to monitor expression levels of thousands of genes at once. ...
and Post-assessment multiple choice questions
... D. Cells that did not take up the plasmid will survive on the medium. E. Each colony began with one antibiotic resistant cell and all cells in the colony are resistant to the antibiotic ampicillin. 12. Which of the following statements concerning development of antibiotic resistance is FALSE? A. It ...
... D. Cells that did not take up the plasmid will survive on the medium. E. Each colony began with one antibiotic resistant cell and all cells in the colony are resistant to the antibiotic ampicillin. 12. Which of the following statements concerning development of antibiotic resistance is FALSE? A. It ...
Document
... Why didn’t Mendel Observe Linkage? • There are 7 chromosomes and 7 genes • Did he get one gene per chromosome? ...
... Why didn’t Mendel Observe Linkage? • There are 7 chromosomes and 7 genes • Did he get one gene per chromosome? ...
Name
... o Monosomy – in diploid organisms, when one chromosome of a pair is missing o Trisomy – in diploid organisms, when there is an extra chromosome of any of the pairs Topic 11: Genetic Engineering A. Genetic engineering is a new field of biology in which genes can be transferred from one organism to an ...
... o Monosomy – in diploid organisms, when one chromosome of a pair is missing o Trisomy – in diploid organisms, when there is an extra chromosome of any of the pairs Topic 11: Genetic Engineering A. Genetic engineering is a new field of biology in which genes can be transferred from one organism to an ...
Y13 Biology Y2 PLCs Student Teacher 1
... If respiration is only anaerobic, pyruvate can be converted to ethanol or lactate using reduced NAD. The oxidised NAD produced in this way can be used in further glycolysis. If respiration is aerobic, pyruvate from glycolysis enters the mitochondrial matrix by active transport. Aerobic respiration i ...
... If respiration is only anaerobic, pyruvate can be converted to ethanol or lactate using reduced NAD. The oxidised NAD produced in this way can be used in further glycolysis. If respiration is aerobic, pyruvate from glycolysis enters the mitochondrial matrix by active transport. Aerobic respiration i ...
Document
... •DNA Pool #5 must be narrowed down to find the individual line with a knockout in At2G37120 ...
... •DNA Pool #5 must be narrowed down to find the individual line with a knockout in At2G37120 ...
DNA …… solving the puzzle of life
... What is the key to this accuracy? Explain using a diagram. If there were many errors, what would be the problem? (Remember that genes often make proteins). How can we tell that an error has been made? ...
... What is the key to this accuracy? Explain using a diagram. If there were many errors, what would be the problem? (Remember that genes often make proteins). How can we tell that an error has been made? ...
Genetic Engineering - Needham Public Schools
... Selective Breeding • Breed only those plants or animals with desirable traits ...
... Selective Breeding • Breed only those plants or animals with desirable traits ...
The Quest for Ancient DNA
... in amber – Plant materials found in ancient tombs – Bacteria – Bones ...
... in amber – Plant materials found in ancient tombs – Bacteria – Bones ...
Nucleus - Control Center of cell
... Every Organism has specific number of chromosomes • Humans have 46 (23 pairs one from each parent) • One pair determines sex. XX or XY… why? • Flies have 4 QuickTime™ and a TIFF (LZW) decompressor are needed to see this picture. ...
... Every Organism has specific number of chromosomes • Humans have 46 (23 pairs one from each parent) • One pair determines sex. XX or XY… why? • Flies have 4 QuickTime™ and a TIFF (LZW) decompressor are needed to see this picture. ...
Quiz: DNA, RNA and Protein
... 11. What kind of bond holds the DNA bases together? 12. A three nucleotide sequence of DNA is called a _______________. 13. How many different amino acids are there? 14. State three differences between DNA and RNA. 15. The base uracil pairs with what DNA nucleotide 16. If the DNA coding strand is GT ...
... 11. What kind of bond holds the DNA bases together? 12. A three nucleotide sequence of DNA is called a _______________. 13. How many different amino acids are there? 14. State three differences between DNA and RNA. 15. The base uracil pairs with what DNA nucleotide 16. If the DNA coding strand is GT ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.