Sir Alec Jeffreys minisatellites
... Unit - 15-400 bp (average about 20). Repeat - Generally 20-50 times (1000-5000 bp long). Location - Generally euchromatic. Examples - DNA fingerprints. Tandemly repeated but often in dispersed clusters. Also called VNTR’s (variable number tandem repeats). Human λ33.1 minisatellite (62 bp) AAGGGTGGGC ...
... Unit - 15-400 bp (average about 20). Repeat - Generally 20-50 times (1000-5000 bp long). Location - Generally euchromatic. Examples - DNA fingerprints. Tandemly repeated but often in dispersed clusters. Also called VNTR’s (variable number tandem repeats). Human λ33.1 minisatellite (62 bp) AAGGGTGGGC ...
Biology Test Topics Chapters 11-12 Slideshows
... Contrast prokaryotic and eukaryotic DNA replication Be able to label any of the diagrams in our DNA packet. Gel electrophoresis: how does it work? What can it be used for? What is the purpose of the electrode? What does the gel do? How do short and long strands of DNA behave differently when moving ...
... Contrast prokaryotic and eukaryotic DNA replication Be able to label any of the diagrams in our DNA packet. Gel electrophoresis: how does it work? What can it be used for? What is the purpose of the electrode? What does the gel do? How do short and long strands of DNA behave differently when moving ...
Biotechnology, Part I
... DNA The gene of interest is pasted into the vector DNA along with a gene for resistance to a specific antibiotic. ...
... DNA The gene of interest is pasted into the vector DNA along with a gene for resistance to a specific antibiotic. ...
Mini lab 11.1 and 11.2
... instructions into proteins requires a series of coordinated steps in transcription and translation. Procedure: 1. Use the data table below. Complete column B by writing the correct mRNA codon for each sequence of DNA bases listed in the column marked DNA Base Sequence. Use the letters A, U, C, G. 2. ...
... instructions into proteins requires a series of coordinated steps in transcription and translation. Procedure: 1. Use the data table below. Complete column B by writing the correct mRNA codon for each sequence of DNA bases listed in the column marked DNA Base Sequence. Use the letters A, U, C, G. 2. ...
Biotechnology and Recombinant DNA I. Tools of Biotechnology
... • phage/viruses • Cosmid • BACs – bacterial artificial chromosomes/YACs – yeast artificial chromosomes – linear plasmids Vector features 1. Origin of replication (ori) and other replication functions for stable maintenance in host cells. Shuttle vector: can replicate in several different species (e. ...
... • phage/viruses • Cosmid • BACs – bacterial artificial chromosomes/YACs – yeast artificial chromosomes – linear plasmids Vector features 1. Origin of replication (ori) and other replication functions for stable maintenance in host cells. Shuttle vector: can replicate in several different species (e. ...
amazing facts about human dna and genome
... cytosine and guanine, or A, T, C, and G. It is the sequence of these bases in the DNA molecules which determines the biochemistry of cells and physiology of organisms. BAC (bacterial artificial chromosome) clones seem to represent human DNA far more faithfully than their YAC or cosmid counterparts a ...
... cytosine and guanine, or A, T, C, and G. It is the sequence of these bases in the DNA molecules which determines the biochemistry of cells and physiology of organisms. BAC (bacterial artificial chromosome) clones seem to represent human DNA far more faithfully than their YAC or cosmid counterparts a ...
GENETICS OF BACTERIA AND VIRUSES. BIOTECHNOLOGY
... Complex transposons vary in length from about 2,000 to more than 40,000 nucleotide pairs and contain insertion sequences (or closely related sequences) at each end, usually as inverted repeats. The entire complex element can transpose as a unit. ...
... Complex transposons vary in length from about 2,000 to more than 40,000 nucleotide pairs and contain insertion sequences (or closely related sequences) at each end, usually as inverted repeats. The entire complex element can transpose as a unit. ...
04 Genetics of Bacteria and Viruses. Biotechnology
... Complex transposons vary in length from about 2,000 to more than 40,000 nucleotide pairs and contain insertion sequences (or closely related sequences) at each end, usually as inverted repeats. The entire complex element can transpose as a unit. ...
... Complex transposons vary in length from about 2,000 to more than 40,000 nucleotide pairs and contain insertion sequences (or closely related sequences) at each end, usually as inverted repeats. The entire complex element can transpose as a unit. ...
Full Text - Harvard University
... the outer reaches of the universe and contemplating how gases revolve around black holes in the centers of quasars: my PhD topic. Had it not been for the discovery of microarrays, I may never have had the opportunity to turn my scientific career on its head and begin training in bioinformatics. The ...
... the outer reaches of the universe and contemplating how gases revolve around black holes in the centers of quasars: my PhD topic. Had it not been for the discovery of microarrays, I may never have had the opportunity to turn my scientific career on its head and begin training in bioinformatics. The ...
EDVOTEK 225 DNA Fingerprinting
... • Restriction enzymes are endonucleases that catalyze cleavage of phosphate bonds • Require Mg-2 for activity • Generate 5’ phosphae and 3’ hydroxyl group • Endonuclease claves at specific sequence of bases. • Produce by bacteria ...
... • Restriction enzymes are endonucleases that catalyze cleavage of phosphate bonds • Require Mg-2 for activity • Generate 5’ phosphae and 3’ hydroxyl group • Endonuclease claves at specific sequence of bases. • Produce by bacteria ...
Taxonomic distribution of Large DNA viruses in the sea
... particles per milliliter of sea water Infect marine organisms from oxygen-producing phytoplankton to whales Regulate the population of many sea organisms and are important effectors of global biogeochemical fluxes Hold a great genetic diversity May significantly contribute to the evolution of microo ...
... particles per milliliter of sea water Infect marine organisms from oxygen-producing phytoplankton to whales Regulate the population of many sea organisms and are important effectors of global biogeochemical fluxes Hold a great genetic diversity May significantly contribute to the evolution of microo ...
1 •Mitosis •Meiosis •Sex and Genetic Variability •Cloning
... •Separating the two copies •Dividing the “parent” cell in half producing two “daughter” cells •Each “daughter” cell has the same genotype as the “parent” cell ...
... •Separating the two copies •Dividing the “parent” cell in half producing two “daughter” cells •Each “daughter” cell has the same genotype as the “parent” cell ...
Gene Technology
... 2. Cut a plasmid (cloning vector) with one restriction site for the restriction enzyme – the plasmid will now have the same sticky ends (plasmid should also be resistant to antibiotic like ampicillin) 3. Mix the foreign DNA with the plasmids 4. Apply DNA ligase ...
... 2. Cut a plasmid (cloning vector) with one restriction site for the restriction enzyme – the plasmid will now have the same sticky ends (plasmid should also be resistant to antibiotic like ampicillin) 3. Mix the foreign DNA with the plasmids 4. Apply DNA ligase ...
ES Cell Targeting Handbook
... location of the mutation. However, homologous recombination occurs about 1000-fold less then random insertion. This is why screening beyond selection for the mere presence of the marker in the target is required. These ES cells once injected into a blastocyst can contribute to development in the emb ...
... location of the mutation. However, homologous recombination occurs about 1000-fold less then random insertion. This is why screening beyond selection for the mere presence of the marker in the target is required. These ES cells once injected into a blastocyst can contribute to development in the emb ...
Chapter 16, Extranuclear inheritance
... – Liver cells have 1000 mitochondria per cell. – Skin cells have 100. – Egg cells have up to 10 million. Human mitochondria have 37 genes. ...
... – Liver cells have 1000 mitochondria per cell. – Skin cells have 100. – Egg cells have up to 10 million. Human mitochondria have 37 genes. ...
Investigation 3 power point
... Asexual reproduction it the production of offspring that does not involve the union of gametes. ...
... Asexual reproduction it the production of offspring that does not involve the union of gametes. ...
Answer Key DNA Review - John Bowne High School
... D) evolution, reproduction, and digestion 24. Researchers have found that formaldehyde and asbestos can alter DNA base sequences. Based on this research, the use of these chemicals has been greatly reduced because they A) may act as fertilizers, increasing the growth of algae in ponds B) have been r ...
... D) evolution, reproduction, and digestion 24. Researchers have found that formaldehyde and asbestos can alter DNA base sequences. Based on this research, the use of these chemicals has been greatly reduced because they A) may act as fertilizers, increasing the growth of algae in ponds B) have been r ...
Pharmacogenetics Glossary
... homozygous - refers to having an identical pair of alleles, one from each parent, as opposed to heterozygous. introns - DNA sequences without instructions for making protein that come between those sequences with instructions for making protein (exons). Introns are not in messenger RNA, and it is no ...
... homozygous - refers to having an identical pair of alleles, one from each parent, as opposed to heterozygous. introns - DNA sequences without instructions for making protein that come between those sequences with instructions for making protein (exons). Introns are not in messenger RNA, and it is no ...
DNA and Central Dogma Study Guide
... b) A ____________________ bond holds the backbone together and a ______________________ bond holds the two strands together at the bases. 8. What is the function of histones? 9. Summarize the flow of genetic information starting with DNA. 10. When does DNA replicate? 11. What does DNA replication ma ...
... b) A ____________________ bond holds the backbone together and a ______________________ bond holds the two strands together at the bases. 8. What is the function of histones? 9. Summarize the flow of genetic information starting with DNA. 10. When does DNA replicate? 11. What does DNA replication ma ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.