here - Norwegian Genomics Consortium
... depending on the type of analysis (Table 1). All common variants described in databases such as dbSNP and our in-house database were filtered out, as well as synonymous variants (not causing amino acid change) and variants in introns and UTR regions except for those affecting canonical splice sites. ...
... depending on the type of analysis (Table 1). All common variants described in databases such as dbSNP and our in-house database were filtered out, as well as synonymous variants (not causing amino acid change) and variants in introns and UTR regions except for those affecting canonical splice sites. ...
In vitro formation of a catabolic plasmid carrying
... gave the strongest signal and this D N A was ligated into HindIII-digested pBR322. After transformation into E. coli strain SIC, ampicillin-resistant colonies were probed with the labelled oligodeoxyribonucleotide and those showing strong hybridization identified. Plasmid D N A was isolated from a s ...
... gave the strongest signal and this D N A was ligated into HindIII-digested pBR322. After transformation into E. coli strain SIC, ampicillin-resistant colonies were probed with the labelled oligodeoxyribonucleotide and those showing strong hybridization identified. Plasmid D N A was isolated from a s ...
EAWAG news 56e: Genomic Islands and Horizontal Gene Transfer
... the recipient bacteria with auxiliary capabilities for infecting eukaryotic hosts or for degrading environmental pollutants. Almost ten years ago, we started to investigate the process of horizontal gene transfer in bacteria (see glossary). Our aim was to estimate how frequent certain types of genes ...
... the recipient bacteria with auxiliary capabilities for infecting eukaryotic hosts or for degrading environmental pollutants. Almost ten years ago, we started to investigate the process of horizontal gene transfer in bacteria (see glossary). Our aim was to estimate how frequent certain types of genes ...
Part 1: Motivation, Basic Concepts, Algorithms
... – Finding good sub-optimum in a reasonable time, rather than spending years on finding, perhaps, the best solution. ...
... – Finding good sub-optimum in a reasonable time, rather than spending years on finding, perhaps, the best solution. ...
pARA and pKAN-R
... naturally in bacterial cells. The plasmids used in molecular biology have been modified through genetic engineering to facilitate gene cloning and protein production (gene expression) in bacteria. Antibiotic resistant genes have been engineered into these plasmids and function as selectable markers— ...
... naturally in bacterial cells. The plasmids used in molecular biology have been modified through genetic engineering to facilitate gene cloning and protein production (gene expression) in bacteria. Antibiotic resistant genes have been engineered into these plasmids and function as selectable markers— ...
Full-Text PDF
... investigated. Only chromosome 24 did not contain any EqERV-beta1 LTR sequences. The largest number of hits (22) was found on chromosome 10, with chromosome 5 harboring six LTRs. Four LTRs, on chromosomes 5 (2×), 7 and 20, were followed by a PBS(Lys3) sequence. 3.4. The LTR of the Horse Endogenous Re ...
... investigated. Only chromosome 24 did not contain any EqERV-beta1 LTR sequences. The largest number of hits (22) was found on chromosome 10, with chromosome 5 harboring six LTRs. Four LTRs, on chromosomes 5 (2×), 7 and 20, were followed by a PBS(Lys3) sequence. 3.4. The LTR of the Horse Endogenous Re ...
Document
... The reference sequence for each human chromosome provides the framework for understanding genome function, variation and evolution. Here we report the finished sequence and biological annotation of human chromosome 1. Chromosome 1 is gene-dense, with 3,141 genes and 991 pseudogenes, and many coding ...
... The reference sequence for each human chromosome provides the framework for understanding genome function, variation and evolution. Here we report the finished sequence and biological annotation of human chromosome 1. Chromosome 1 is gene-dense, with 3,141 genes and 991 pseudogenes, and many coding ...
P site
... the synthesis of one polypeptide. Bacterial chromosome Consists of a single molecule of double-stranded deoxyribonucleic acid (DNA) in ring shape which is in association with histone like proteins. A bacterium contains one chromosome. It contains hereditary information which is passed from one gener ...
... the synthesis of one polypeptide. Bacterial chromosome Consists of a single molecule of double-stranded deoxyribonucleic acid (DNA) in ring shape which is in association with histone like proteins. A bacterium contains one chromosome. It contains hereditary information which is passed from one gener ...
Chromosome challenge activity pack
... also controls the cells in your body. Sometimes there are bits of information missing, or it is a bit scrambled, and this can cause illness. The DNA in your cells is squashed into packages called chromosomes. Scientists study DNA and chromosomes to learn about human health. They often compare DNA fr ...
... also controls the cells in your body. Sometimes there are bits of information missing, or it is a bit scrambled, and this can cause illness. The DNA in your cells is squashed into packages called chromosomes. Scientists study DNA and chromosomes to learn about human health. They often compare DNA fr ...
Detection of Mosaicism by Augmented Exome
... strategies. However, routine detection of mosaicism in conventional genome and exome sequencing is hampered by multiple factors. Assumptions inherent to genotyping and filtration strategies utilized by both genome and exome analysis often result in the dismissal or outright failure to call variants ...
... strategies. However, routine detection of mosaicism in conventional genome and exome sequencing is hampered by multiple factors. Assumptions inherent to genotyping and filtration strategies utilized by both genome and exome analysis often result in the dismissal or outright failure to call variants ...
NATIONAL UNIVERSITY OF SINGAPORE DEPARTMENT OF BIOLOGICAL SCIENCES ADVANCED PLACEMENT TEST
... B. Conjugation is the direct transmission of DNA from one bacteria cell to another, but is not a replicative process. C. Plasmid DNA transfer from donor to recipient by conjugation is usually initiated at specific site, known as oriT. D. F plasmid can integrate into E. coli chromosome. E. Competence ...
... B. Conjugation is the direct transmission of DNA from one bacteria cell to another, but is not a replicative process. C. Plasmid DNA transfer from donor to recipient by conjugation is usually initiated at specific site, known as oriT. D. F plasmid can integrate into E. coli chromosome. E. Competence ...
pdf
... When they first form, allopolyploids are typically, for many characters and traits, intermediate between their two parents, and they are in instant competition if they occur sympatrically with their parents. They may also lack an ecological niche and/or experience low rates of pollination as a resul ...
... When they first form, allopolyploids are typically, for many characters and traits, intermediate between their two parents, and they are in instant competition if they occur sympatrically with their parents. They may also lack an ecological niche and/or experience low rates of pollination as a resul ...
Lateral gene transfer between prokaryotes and multicellular
... implying that the transfer is not recent – and pseudogenization would be expected in the absence of positive selection for function. The aphid study [9] is one of several recent papers describing lateral transfer within symbiosis. Rumpho et al. [10] found evidence for LGT between two eukaryotes, the ...
... implying that the transfer is not recent – and pseudogenization would be expected in the absence of positive selection for function. The aphid study [9] is one of several recent papers describing lateral transfer within symbiosis. Rumpho et al. [10] found evidence for LGT between two eukaryotes, the ...
BMC Biology - BioMedSearch
... implying that the transfer is not recent – and pseudogenization would be expected in the absence of positive selection for function. The aphid study [9] is one of several recent papers describing lateral transfer within symbiosis. Rumpho et al. [10] found evidence for LGT between two eukaryotes, the ...
... implying that the transfer is not recent – and pseudogenization would be expected in the absence of positive selection for function. The aphid study [9] is one of several recent papers describing lateral transfer within symbiosis. Rumpho et al. [10] found evidence for LGT between two eukaryotes, the ...
Poliammine, evoluzione e patogenicità in Shigella spp
... expression of virulence genes residing on a large plasmid and on the chromosome. The genomes of Shigella and E. coli, its commensal ancenstor, are colinear and highly homologous. Critical events in the evolution of Shigella have been the acquisition of the virulence plasmid through lateral gene tran ...
... expression of virulence genes residing on a large plasmid and on the chromosome. The genomes of Shigella and E. coli, its commensal ancenstor, are colinear and highly homologous. Critical events in the evolution of Shigella have been the acquisition of the virulence plasmid through lateral gene tran ...
Mendelian Genetics part 4
... b. They may experience problems with possible bleeding to death. c. This was a disorder associated with the “Royal Blue-Bloods of Europe” – They were inbreeding to keep the crown “In the Family”. d. Treatment? These individuals have to keep AHF with them at all times in case they get hurt. If they d ...
... b. They may experience problems with possible bleeding to death. c. This was a disorder associated with the “Royal Blue-Bloods of Europe” – They were inbreeding to keep the crown “In the Family”. d. Treatment? These individuals have to keep AHF with them at all times in case they get hurt. If they d ...
Document
... The genetic code is said to be degenerate because in many cases more then one codon is assigned to a single amino acid, and, in addition, several codons can pair with more then on anticodon (wobble) ...
... The genetic code is said to be degenerate because in many cases more then one codon is assigned to a single amino acid, and, in addition, several codons can pair with more then on anticodon (wobble) ...
Segmented Arrangement of Borrelia duttonii DNA
... virtually comigrated in conventional 1 % agarose gels, formed a 'family' with apparent sizes ranging from 24 kb to 150 kb when separated by prolonged, low-voltage electrophoresis on soft gels followed by Southern blotting (see Fig. 1b, bands C to I). ;1DNA oligomers were used to estimate the sizes o ...
... virtually comigrated in conventional 1 % agarose gels, formed a 'family' with apparent sizes ranging from 24 kb to 150 kb when separated by prolonged, low-voltage electrophoresis on soft gels followed by Southern blotting (see Fig. 1b, bands C to I). ;1DNA oligomers were used to estimate the sizes o ...
Untitled
... process. Gradually, we got better at reading. The first lucky organism to have its whole genome read was the bacterium Haemophilus influenzae. That sequence was published in 1995, clocking in at just 1.8 million letters compared to our three billion. Yeast followed in 1996 and a tiny worm known as C ...
... process. Gradually, we got better at reading. The first lucky organism to have its whole genome read was the bacterium Haemophilus influenzae. That sequence was published in 1995, clocking in at just 1.8 million letters compared to our three billion. Yeast followed in 1996 and a tiny worm known as C ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.