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The aquaporin-Z water channel gene of Escherichia co/i
The aquaporin-Z water channel gene of Escherichia co/i

... c®ulatory elements within the 500 bp preceding the aqpZ ORF. The presence of possible repetitive extragenic palindromic (REP) sequences following the aqpZ stop codon was investigated with SiteFinder, a computer program devised by Webb Miller and Kenneth Rudd. Possible extensive secondary structur ...
MGC premier full length cDNA and ORF clones
MGC premier full length cDNA and ORF clones

Lecture7
Lecture7

... • Detect potential coding regions by looking at ORFs – A region of length n is comprised of (n/3) codons – Stop codons break genome into segments between consecutive Stop codons – The subsegments of these that start from the Start codon (ATG) are ORFs ATG ...
annotation and analysis of newly discovered mycobacteriophage
annotation and analysis of newly discovered mycobacteriophage

... host. We are analyzing two novel mycobacteriophage, Firecracker and Dori, which were isolated on the UCSC campus using Mycobacterium Smegmatis as the viral host. After multiple rounds of plaque purification, we performed electron microscopy and observed that Dori has a typical siphoviral morphology ...
DNA Recombination - Home - KSU Faculty Member websites
DNA Recombination - Home - KSU Faculty Member websites

... exchange between molecules with extended sequence homology. For example, transformation and conjugation between related bacterial strains. Site-specific recombination refers to DNA recombination between molecules that shared limited regions of sequence homology. ...
DETERMINING THE METHOD OF DNA REPLICATION LAB
DETERMINING THE METHOD OF DNA REPLICATION LAB

... After the publication of the structure of DNA, several possible hypotheses were advanced to describe how the DNA replicated. Three hypotheses were considered the most likely candidates to correctly explain replication: conservative, semiconservative, and dispersive. During conservative replication, ...
Evolution, Body Plans, and Genomes
Evolution, Body Plans, and Genomes

gene expression_hour 1 - study
gene expression_hour 1 - study

... transformation occur. So, the transformation agent is DNA! ...
Lacroix_Insyght navigating amongst abundant - Migale
Lacroix_Insyght navigating amongst abundant - Migale

... are ‘paralogues’ and others correspond to genes of the reference genome that have several ‘homologues’ in the compared genome (see the database and pipeline section). For the sake of simplicity, we will use the general term ‘homologue’ to describe all evolutionary relationships thereafter. The user ...
Directions: Each of the questions or incomplete statements below is
Directions: Each of the questions or incomplete statements below is

... 31.Crossing-over is a process that involves the (A) exchange of genetic material between chromosomes that are homologous (B) exchange of genetic material between chromosomes that are not homologous (C) random segregation of genes on different chromosomes (D) random segregation of genes on homologous ...
טקס חלוקת מלגות ע"ש אורן ברקו ז"ל 2006
טקס חלוקת מלגות ע"ש אורן ברקו ז"ל 2006

... coexisting ecotypes well adapted to certain microhabitats. The results of these studies led to two publications (Journal of Limnology and Oceanography and Journal of Environmental Microbiology) and a third publication currently in preparation. Environmental, high molecular weight genomic DNA from tw ...
Junk DNA - repetitive sequences
Junk DNA - repetitive sequences

LiMA overview
LiMA overview

... LiMA – advantages compared to direct detection of bacterial genomes by PCR • LiMA is generic – all bacteria tested contain NAD-dependent DNA ligase. It is difficult to ensure that direct PCR is generic. • LiMA is more sensitive than direct PCR. LiMA involves lysis of the bacilli and release of many ...
Molecular Biology -
Molecular Biology -

...  amino acid sequence in a polypeptide which folds into a ____________ translation  structure and function of the protein (e.g. normal hemoglobin vs. sickle cell hemoglobin)  person's characteristics or traits (e.g. normal health vs. sickle cell anemia) 2. The double helix structure of DNA, transc ...
File
File

... the sperm or egg cell there are 23 singular chromosomes rather than 23 pairs of chromosomes. That is because only one out of each pair of chromosomes will be passed to your child (to prevent the child from having double the genes it needs!). 23 singular chromosomes in the sperm cell will combine wit ...
From Communication to DNA Sequencing
From Communication to DNA Sequencing

... If the erasure probability is less than 1/3, then noiseless performance can be achieved. A separation architecture is optimal: ...
STATION 1: Nucleic acids
STATION 1: Nucleic acids

... 13) What is the most likely explanation for this base composition? 14) A mutant of E. coli with a heat-sensitive DNA ligase (25°C permissive, 37°C nonpermissive) has been used to show that DNA synthesis is discontinuous. Examination of DNA replication in the presence of [3H]-thymidine in the mutant ...
Bacterial Identification
Bacterial Identification

Recombinant DNA - Rose
Recombinant DNA - Rose

press alert - the Gregor Mendel Institute
press alert - the Gregor Mendel Institute

... transposons in gamete companion cells generates mobile signals - small RNAs - that move into the gametes to immunize against transposon activation over the plant sexual cycles. What are transposons and what is transposon silencing through DNA methylation? Transposons are parasitic mobile DNA element ...
CONNECTION: Many viruses cause disease in animals and plants
CONNECTION: Many viruses cause disease in animals and plants

... – Unique noncoding DNA – Repetitive DNA – Found in centromeres and telomeres – Found dispersed throughout the genome, related to transposable elements that can move or be copied from one location to another Copyright © 2009 Pearson Education, Inc. ...
Big Data Study - Open Medicine Foundation
Big Data Study - Open Medicine Foundation

... discovery phase could use various mass spectroscopy methods that are now quite advanced. Unique protein modifications could also be used. Many Physicians and researchers speculate that some microbe is the initiating event of ME/CFS. Although this supposed organism(s) may not continue to be present, ...
Mitosis
Mitosis

... 17. Most plants appear green because chlorophyll does not absorb _______________ light. 18. What gas is produced as a by-product of photosynthesis? __________________. 19. Describe the light-dependent and light-independent reactions and know where they occur. The light dependent reaction uses ______ ...
Final Exam answer key
Final Exam answer key

Mitosis
Mitosis

... 17. Most plants appear green because chlorophyll does not absorb _______________ light. 18. What gas is produced as a by-product of photosynthesis? __________________. 19. Describe the light-dependent and light-independent reactions and know where they occur. The light dependent reaction uses ______ ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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