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8.
8.

... the kaiC homologs in hyperthermophiles; together, these two genes might have an important role in signal transduction in these organisms. COG2250 and COG1895 – putative molecular chaperones important for hyperthermophily COG2250 comes even closer to being a true ‘hyperthermophilic signature’ because ...
SBI4U- Molecular Genetics
SBI4U- Molecular Genetics

... Explain what this itatlicized term means, and indicate the significance of this for genetic research. (3 marks) Alternative splicing refers to the fact that when introns are cut out, the exons (coding regions) can be rearranged or even omitted in different ways producing different mRNA transcripts, ...
Exam II Notes DNA
Exam II Notes DNA

... Mom gave you finds the double #1 chromosome that Dad gave you. All 23 pairs line up at the metaphase plate. As anaphase 1 begins one of each homologous chromosome goes to each side. (So there is no ripping of the chromosomes after the first meiotic division.) Now there are 23 double chromosomes at e ...
Sequencing Crop Genomes - Tropical Life Sciences Research
Sequencing Crop Genomes - Tropical Life Sciences Research

... sequencing (NGS) technologies provide opportunities to develop millions of novel markers, as well as the identification of agronomically important genes (Edwards & Batley 2010). SNPs now dominate over other molecular marker applications, with the advancement in sequencing technology. Traditionally, ...
BIOL 221_syllabus_part1_2010
BIOL 221_syllabus_part1_2010

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central dogma of molecular biology - Rose

... alter the tightness of the winding, and can pass one strand through another. Eukaryotic DNA replication Eukaryotic cells are much more complex than prokaryotic cells, and contain much more DNA. Human cells contain more than 1000 times more DNA than E. coli. The polymerase responsible for human DNA r ...
03-131 Genes, Drugs, and Diseases Exam 2 – F2015 Name:____________________
03-131 Genes, Drugs, and Diseases Exam 2 – F2015 Name:____________________

... Instructions: This exam has 5 pages. Total points=100. Use the space provided, or the back of the previous page if necessary. On questions with choices, your answer that gives the highest grade will be used. A. Multiple Choice and fill in the blanks (15 points) 1. The increase in the rate of an enzy ...
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TRANSCRIPTION TO TRANSLATION

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SBI-4U1 Exam Review

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... The genome consists of the sum total of an organism's chromosomes. Each chromosome contains hundreds and sometimes thousands of genes, segments of DNA that code for a polypeptide or RNA, and a large amount of DNA with no known function. ...
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SBI-4U1 Exam Review

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...  They protect the bacterial cell by cutting foreign DNA from other organisms or phages  Hundreds have been identified, they are highly specific and recognize a particular sort DNA sequence or Restriction site  Most restriction sites are symetrical (meaning the same sequence whether it is read 5’- ...
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Horizontal gene transfer and the origin of species: lessons from

... insertional inactivation, so that the incorporated genes are fixed. On the evolutionary scale, the acquisition of pathogenicity islands occurs at a much slower pace than that of plasmids, and would obviously create new bacterial subspecies (i.e. the recombinants have discernibly different phenotypes ...
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Gene discovery in in the parasitic plant Ipomoeae hederacea expressed sequence tags.

... physiological consequences of adopting the parasitic habit, we are investigating the function and fate of genes that are expressed in tissues that have undergone reduction or have been lost in the parasite. During the summer session of 2008, the Biology-STEP molecular research team examined populati ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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