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DNA notes 2015 - OG
DNA notes 2015 - OG

... Fingerprinting takes the DNA out of a cell and separates it. This will allow investigators to distinguish body cells of different individuals (since they are unlikely to have the same DNA) Cloning – take the DNA out of one of your cells then take the DNA out of a zygote (fertilized egg). Put the DNA ...
The Secret of DNA - University Writing
The Secret of DNA - University Writing

... The lab at King's College, the closest of the three labs to discovering the structure of DNA, was headed by New Zealand-born Maurice Hugh Wilkins and his "assistant" Rosalind Elise Franklin, but their inability to work together slowed their progress. Wilkins had brought Franklin to King's college to ...
Genetics problems - University of Toronto Mississauga
Genetics problems - University of Toronto Mississauga

... 6. Which of the following is due to the principle of segregation? a. Each gamete has an equal chance of getting either allele for a gene. b. Each gamete formed in an organism will have one copy of each gene. c. Half of the gametes produced by an individual that is AaBb will be AB. d. Each gamete for ...
DNA Replication, Repair, and Recombination
DNA Replication, Repair, and Recombination

... versions of flagellin, H1 and H2 only one of the two is expressed switch every 1000 cell generations, phase variation may help evade host immune response H2 is linked to rh1, that encodes a repressor for H1 Expression of H2-rh1 unit is controlled by a 995bp segment that contains 1. Promoter for H2-r ...
The Sequence Manipulation Suite—a collection of JavaScript prog
The Sequence Manipulation Suite—a collection of JavaScript prog

Proving that DNA Replication is Semiconservative
Proving that DNA Replication is Semiconservative

... to one containing 14N as the sole nitrogen source. From this point on, all the DNA synthesized by the bacteria would incorporate 14N, rather than 15N, so that the daughter DNA strands would contain only 14N. As the bacteria continued to grow and replicate their DNA in the 14Ncontaining medium, sampl ...
Chp 18 Viruses and Bacteria
Chp 18 Viruses and Bacteria

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Colony PCR from Yeast or Bacteria
Colony PCR from Yeast or Bacteria

Genetics and Genomics in Medicine Chapter 6 Questions Multiple
Genetics and Genomics in Medicine Chapter 6 Questions Multiple

... Which, if any, of the following descriptions is false? a) Genomic imprinting in mammals really means that a very few genes are expressed from one allele only, according to the sex of the parent. b) Imprinted genes are often found in clusters of genes, many of which are imprinted. c) means that in a ...
biotechnology
biotechnology

... • Genome variations are differences in the sequence of DNA among individuals. They include both polymorphisms and mutations. • A polymorphism is a clinically harmless DNA variation. It often occurs in the intervening sequences that do not code for proteins • Mutation refers to an infrequent potentia ...
Lecture 13 Lytic vs. Lysogenic cycles:
Lecture 13 Lytic vs. Lysogenic cycles:

... The choice of the phage to enter the lytic or lysogenic cycle comes down to the physiological condition of the bacteria. If the bacteria is healthy enough to sustain the phage it will enter the lysogenic cycle and use the bacteria's machinery. However, if the bacteria is lacking in nutrients and unh ...
Genetics
Genetics

... 2. What type of RNA is involved in transcription? 3. In what organelle does translation occur? 4. What must happen to the newly formed protein before it can begin to work? 92. When a pure-breeding black cat was mated with a pure-breeding white cat, all the kittens were black. Which fur colour, black ...
Honors Genetics: Senior Exam Review Chapter 1: Introduction to
Honors Genetics: Senior Exam Review Chapter 1: Introduction to

... What is the order of steps? What is the general description of chromosome action in meiosis? Vocabulary Review MONAD GENETIC VARIATION DYAD CROSSING OVER TETRAD Know the similarities and differences between SPERMATOGENESIS and OOGENSIS. Understand the impact that certain activities can have on egg a ...
Mutation PPT
Mutation PPT

... unites with a normal one, the offspring will have an abnormal chromosome number, known as aneuploidy • If the chromosome is present in triplicate in the zygote, (the cell has a total of 2n + 1 chromosomes) then the cell is trisomic for that chromosome • trisomy 21 = Down syndrome ...
Non-small-cell lung carcinoma
Non-small-cell lung carcinoma

... derived from most of the known genes and non-coding regions of the genome, printed on a glass slide. 3. The fluorescence intensity of the test and of the reference DNA is then measured, to calculate the ratio between them and subsequently the copy number changes for a particular location in the geno ...
Homework Assignment #1
Homework Assignment #1

... b. You isolate clones for each of these bands. Two correspond to the ACT1 and ACT2 genes you have already identified. The third you name ACT3. Now you prepare labeled probes specific for each individual actin gene (i.e., they will not cross-hybridize with either of the other actin genes) and use the ...
Chapter04_Outline
Chapter04_Outline

... differences in SSRs and SNPs • Restriction enzyme cleavage of polymorphic alleles that are different in RFLP pattern produces different size fragments by gel electrophoresis ...
Chapter 10
Chapter 10

... into fragments of the desired size by physical means, by treatment with restriction enzymes that have infrequent cleavage sites (for example, enzymes such as NotI and SfiI), or by treatment with ordinary restriction enzymes under conditions in which only a fraction of the restriction sites are cleav ...
BISC-576 Practical Statistics and Bioinformatics Instructors:
BISC-576 Practical Statistics and Bioinformatics Instructors:

... can be annotated or predicted based on structural homology, evolutionary conservation, electrostatic potential, and other properties. (Chap 2, Introduction to Proteins) RNA folding and sequence-dependent DNA shape: While RNA and DNA have very similar chemical properties, they have very different bio ...
student - Shawnee Science
student - Shawnee Science

Genome sequencing and analysis of Aspergillus oryzae
Genome sequencing and analysis of Aspergillus oryzae

... programs, algorithms and symbols are same with those in Fig. 3. Supplementary Figure S6. Phylogenetic analysis of metabolic genes. Phylogenetic relationship of pyruvate decarboxylase (a), saccharopine dehydrogenase, homoaconitase and saccharopine dehydrogenase (NADP+, L-glutamate forming) in lysine ...
14 Alignment 3(1)
14 Alignment 3(1)

... line contains information on the number of identical residues in this alignment (Identities), the number of conservative substitutions (Positives), and if applicable, the number of gaps in the alignment. Finally, the actual alignment is shown, with the query on top, and the database match is labeled ...
A VIEW OF GENETICS.
A VIEW OF GENETICS.

... shown that this inherited trait was transmitted from one pneumococcal strain to another by DNA. The general transmission of other traits by the same mechanism (25) can only mean that DNA comprises the genes (b). ...
doc BIOL 112 Course Summary 2013
doc BIOL 112 Course Summary 2013

... High surface area to volume ratio is ideal for diffusion Eggs (one cell) are large because most of the volume is storing food material All organelles in eukaryotic cells are connected by vesicles that bud off of one compartment and fuse to the next, moving molecules between organelles as needed o Or ...
современные проблемы молекулярной биологии
современные проблемы молекулярной биологии

... D process by which a gene's information is converted into the structures and functions of a cell E All of these above 57. What is "transcription" of DNA? A coping codes into codones B pre-mRNA synthesis C matured RNA synthesis D protein synthesis E RNA polymerase 58. What is "translation" of DNA? A ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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