Recombination and clonal groupings within Helicobacter pylori from
... isolated from East Asians and Europeans (Atherton et al ., 1995; Ito et al ., 1998; Pan et al ., 1998; van Doorn et al ., 1998a), suggesting that recombination is rare between bacteria from different continents or that particular alleles are selected for in certain populations. However, a different ...
... isolated from East Asians and Europeans (Atherton et al ., 1995; Ito et al ., 1998; Pan et al ., 1998; van Doorn et al ., 1998a), suggesting that recombination is rare between bacteria from different continents or that particular alleles are selected for in certain populations. However, a different ...
DNA Hybridization: A Decade of Molecular Discourse in Hominoid
... or if either of them might be closer to humans than to each other. This takes us to Benveniste and Todaro's (1976) study. They also made only one DNADNA hybrid for each pairwise comparison, but their data did not resolve the Homo-PanGorifla trichotomy. So, when Goodman and Cronin concluded in 1982 t ...
... or if either of them might be closer to humans than to each other. This takes us to Benveniste and Todaro's (1976) study. They also made only one DNADNA hybrid for each pairwise comparison, but their data did not resolve the Homo-PanGorifla trichotomy. So, when Goodman and Cronin concluded in 1982 t ...
Genomic imprinting of a placental lactogen gene in Peromyscus
... by using RT-PCR primers that amplified both genes, and exploiting a restriction fragment length polymorphism between them. We observed that the imprinted transcript was only detected after E12, similar to the rat rPl1-v gene (Fig. 3). For this reason we have designated the imprinted transcript as pP ...
... by using RT-PCR primers that amplified both genes, and exploiting a restriction fragment length polymorphism between them. We observed that the imprinted transcript was only detected after E12, similar to the rat rPl1-v gene (Fig. 3). For this reason we have designated the imprinted transcript as pP ...
BMC Microbiology - ORBi
... the same strains in presence of pancreatic juice with a decrease of less than one log. Survival of strain FR49/f/2 was better than FR41/2 with a decrease of 3 logarithms (in presence of 1% bile salts) and almost 2 logarithms (in presence of 0.5% bile salts). The genotypic analyses using total DNA-DN ...
... the same strains in presence of pancreatic juice with a decrease of less than one log. Survival of strain FR49/f/2 was better than FR41/2 with a decrease of 3 logarithms (in presence of 1% bile salts) and almost 2 logarithms (in presence of 0.5% bile salts). The genotypic analyses using total DNA-DN ...
File - Integrated Science
... Targeted deletion by homologous recombination (KNOCKOUT MICE) - We’ll discuss next time Specific mutational changes can be made Time consuming and limited to certain organisms Antisense RNA Variable effects and mechanism not understood ...
... Targeted deletion by homologous recombination (KNOCKOUT MICE) - We’ll discuss next time Specific mutational changes can be made Time consuming and limited to certain organisms Antisense RNA Variable effects and mechanism not understood ...
Kevin Ann Hunt Biol 509- Molecular Biology and Applications 1
... conferred resistance to X. perforans, although to a lesser extent than other bacteria. EFR was less effective in reducing X. perforans than the Bs2 gene from pepper (Fig. 4c; Supp. Fig. 3). The Bs2 gene encodes an R protein specific for an effector conserved in ...
... conferred resistance to X. perforans, although to a lesser extent than other bacteria. EFR was less effective in reducing X. perforans than the Bs2 gene from pepper (Fig. 4c; Supp. Fig. 3). The Bs2 gene encodes an R protein specific for an effector conserved in ...
(BACTERIOCIN-LIKE INHIBITORY SUBSTANCES) PRODUCED BY
... alternative agents either in food biopreservation or in prevention/treatment of bacterial infections due to their antimicrobial action against human, plant and animal pathogens, showing potential biotechnological applications. Bacteriocin-like inhibitory substances (BLIS) are antimicrobial agents si ...
... alternative agents either in food biopreservation or in prevention/treatment of bacterial infections due to their antimicrobial action against human, plant and animal pathogens, showing potential biotechnological applications. Bacteriocin-like inhibitory substances (BLIS) are antimicrobial agents si ...
Epigenetic Inactivation of Chalcone Synthase-A
... study, we investigated the mechanisms underlying the reversion of the PTGS phenotype (white flowers) to the normal phenotype (purple flowers) by comparing the C001 and C002 lines. Transcription run-on assays were performed to determine the activity of both the CHS-A transgene and the CHS-A endogene ...
... study, we investigated the mechanisms underlying the reversion of the PTGS phenotype (white flowers) to the normal phenotype (purple flowers) by comparing the C001 and C002 lines. Transcription run-on assays were performed to determine the activity of both the CHS-A transgene and the CHS-A endogene ...
Origin and Distribution of Calvin Cycle Fructose and
... could be the active holoenzyme; however, post-translational processing cannot be ruled out. ...
... could be the active holoenzyme; however, post-translational processing cannot be ruled out. ...
Retrovirus Integration Database (RID): a public database for
... can be accessed in Data Submission tab (Fig. 1). Generally speaking, only data from published peer-reviewed studies will be accepted and made available on the website. We reserve the right not to post data if inspection of the submitted data shows that there are obvious problems with the dataset. I ...
... can be accessed in Data Submission tab (Fig. 1). Generally speaking, only data from published peer-reviewed studies will be accepted and made available on the website. We reserve the right not to post data if inspection of the submitted data shows that there are obvious problems with the dataset. I ...
Newton E. Morton - The American Society of Human Genetics
... practical size”. Using the LOD tests Steinberg and Morton (7) found no significant evidence for that subsample or overall. Finally (8) I reviewed evidence of human linkage published before 1957. Traits were limited to complete dominance or recessivity. Markers were limited to colour blindness on the ...
... practical size”. Using the LOD tests Steinberg and Morton (7) found no significant evidence for that subsample or overall. Finally (8) I reviewed evidence of human linkage published before 1957. Traits were limited to complete dominance or recessivity. Markers were limited to colour blindness on the ...
Author`s personal copy - Real Jardín Botánico
... DNA sequences were edited manually using the Phylogenetic Data Editor (PhyDEÒ, www.phyde.de). Sequences were crosschecked via BLASTn (local Blast-search and via http:// www.ncbi.nlm.nih.gov/BLAST, www.COSMOSS.org and DOE-Joint Genome Institute). Sequences were manually aligned (as far as possible) a ...
... DNA sequences were edited manually using the Phylogenetic Data Editor (PhyDEÒ, www.phyde.de). Sequences were crosschecked via BLASTn (local Blast-search and via http:// www.ncbi.nlm.nih.gov/BLAST, www.COSMOSS.org and DOE-Joint Genome Institute). Sequences were manually aligned (as far as possible) a ...
Cytogenetic and AZF microdeletions on the Y chromosome of
... by these primer sets have been associated with male infertility [12, 16, 19-27]. The primers have been combined into five Multiplex Master Mix sets for use in multiplex PCR. This makes it possible to determine the presence or absence of all 20 STS by performing five concurrent PCR amplifications (Fi ...
... by these primer sets have been associated with male infertility [12, 16, 19-27]. The primers have been combined into five Multiplex Master Mix sets for use in multiplex PCR. This makes it possible to determine the presence or absence of all 20 STS by performing five concurrent PCR amplifications (Fi ...
Uria_et_al_2006 _ADH paper
... marine organisms. Enzymes belong to the most interesting natural products from both scientific and industrial perpectives. They are enjoying increasing popularity in the chemical and pharmaceutical industries as environmental friendly, economical, and clean catalysts (Wahler and Reymond, 2001). By e ...
... marine organisms. Enzymes belong to the most interesting natural products from both scientific and industrial perpectives. They are enjoying increasing popularity in the chemical and pharmaceutical industries as environmental friendly, economical, and clean catalysts (Wahler and Reymond, 2001). By e ...
Analysis of Gene expression data using MATLAB Software
... GRASP) to detect significant biclusters from large microarray datasets. The method has two major steps. First, high quality bicluster seeds are generated by means of k-means[10] clustering. In the second step, these seeds are grown using the Reactive GRASP, in which the basic parameter that defines ...
... GRASP) to detect significant biclusters from large microarray datasets. The method has two major steps. First, high quality bicluster seeds are generated by means of k-means[10] clustering. In the second step, these seeds are grown using the Reactive GRASP, in which the basic parameter that defines ...
A phylogenomic study of DNA repair genes, proteins
... VC-16, DSM4304 Eukaryote S. cereÕisiae S288C ...
... VC-16, DSM4304 Eukaryote S. cereÕisiae S288C ...
The genomic landscape of meiotic crossovers and gene
... genotypes. Since all three parental lines are highly inbred, and hence homozygous throughout their genomes, the presence of heterozygous marker calls can only be explained by misaligned reads that result from unknown rearrangements or repeats. This is especially critical for Ler and Cvi, where genom ...
... genotypes. Since all three parental lines are highly inbred, and hence homozygous throughout their genomes, the presence of heterozygous marker calls can only be explained by misaligned reads that result from unknown rearrangements or repeats. This is especially critical for Ler and Cvi, where genom ...
Microarray data normalization and data transformation
... There are a number of schools of thought regarding the estimation of background for DNA microarrays (1) Not to calculate a background value • But rather to try to estimate it using statistical techniques • Argument – subtracting background introduces significant variance into the estimation of hybri ...
... There are a number of schools of thought regarding the estimation of background for DNA microarrays (1) Not to calculate a background value • But rather to try to estimate it using statistical techniques • Argument – subtracting background introduces significant variance into the estimation of hybri ...
A type III-like restriction endonuclease functions as a major barrier to
... and HsdM. Alignment of the HsdS protein sequences from NCTC 8325 (parent of RN4220, clonal complex CC8) with those of the CC30 strain UAMS-1 (24) and CC5 strain SA564 (25) reveals important differences in their respective target recognition domains (TRDs) (Fig. S1). Each TRD binds to part of the rec ...
... and HsdM. Alignment of the HsdS protein sequences from NCTC 8325 (parent of RN4220, clonal complex CC8) with those of the CC30 strain UAMS-1 (24) and CC5 strain SA564 (25) reveals important differences in their respective target recognition domains (TRDs) (Fig. S1). Each TRD binds to part of the rec ...
Analyzing the Changes in DNA Flexibility Due to Base Modifications
... Finally, we will perform the production simulations in which we remove the restraints from the DNA. The DNA will need to relax for some time after removing the restraints, at least 10 ns. To get meaningful results, this simulation will need to be run for about 100 ns, which will take around 3 days u ...
... Finally, we will perform the production simulations in which we remove the restraints from the DNA. The DNA will need to relax for some time after removing the restraints, at least 10 ns. To get meaningful results, this simulation will need to be run for about 100 ns, which will take around 3 days u ...
Distinguishing Different DNA Heterozygotes by
... under the primers and lead to undesired allele-specific PCR. The same concern applies to internal primers used for sequencing or extension reactions. These caveats aside, some PCR-based methods, including sequencing, will identify and distinguish polymorphisms that are missed by restriction fragment ...
... under the primers and lead to undesired allele-specific PCR. The same concern applies to internal primers used for sequencing or extension reactions. These caveats aside, some PCR-based methods, including sequencing, will identify and distinguish polymorphisms that are missed by restriction fragment ...
HiPer® Bacterial Conjugation Teaching Kit
... elements. The factors (plasmids) contain about 20-30 genes, most of which are associated with conjugation. These genes encode enzymes that replicate DNA during conjugation and structural proteins needed to synthesize special pili at the cell surface. Known as F pili or sex pili, these hairlike fibre ...
... elements. The factors (plasmids) contain about 20-30 genes, most of which are associated with conjugation. These genes encode enzymes that replicate DNA during conjugation and structural proteins needed to synthesize special pili at the cell surface. Known as F pili or sex pili, these hairlike fibre ...
Origin of New Genes: Evidence from Experimental
... each of the processes in new gene evolution. A serious challenge is that many of the early features of evolution of new genes have been lost in the long history of the genes comprising a most common data sources in molecular research, because most genes available for analysis are very old. For examp ...
... each of the processes in new gene evolution. A serious challenge is that many of the early features of evolution of new genes have been lost in the long history of the genes comprising a most common data sources in molecular research, because most genes available for analysis are very old. For examp ...
Functional and ecological impacts of horizontal gene transfer in
... at the genomic level, and here HGT has emerged as a clear factor in the adaptation to various stresses. Nutritionally, two of the major limitations for marine microbes are iron and nitrogen [40]. Iron-stress especially has been the focus of recent attention, as it is known to be the limiting nutrien ...
... at the genomic level, and here HGT has emerged as a clear factor in the adaptation to various stresses. Nutritionally, two of the major limitations for marine microbes are iron and nitrogen [40]. Iron-stress especially has been the focus of recent attention, as it is known to be the limiting nutrien ...
A machine learning approach to gene expression data analysis
... Reasons to apply RS ensembles to the bio-molecular diagnosis of tumors • Gene expression data are usually very high dimensional, and RS ensembles reduce the dimensionality and are effective with high dimensional data (Skurichina and Duin, 2002) • Co-regulated genes show correlated gene expression l ...
... Reasons to apply RS ensembles to the bio-molecular diagnosis of tumors • Gene expression data are usually very high dimensional, and RS ensembles reduce the dimensionality and are effective with high dimensional data (Skurichina and Duin, 2002) • Co-regulated genes show correlated gene expression l ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.