Marine integrons containing novel integrase genes
... and Maruyama, 2010). This unique integron feature creates a system that provides for an enormous pool of adaptive genes to be mobilized, rearranged and disseminated among environmental bacteria. Indeed, the reservoir of adaptive genes capable of being mobilized by integrons has as yet no known upper ...
... and Maruyama, 2010). This unique integron feature creates a system that provides for an enormous pool of adaptive genes to be mobilized, rearranged and disseminated among environmental bacteria. Indeed, the reservoir of adaptive genes capable of being mobilized by integrons has as yet no known upper ...
1 Mathematical Population Genetics Introduction to the
... mt DNA sequences from 63 individuals. The sample approximated a random sample of individuals in the tribe, to the extent to which this can be experimentally arranged. Each sequence is the first 360 basepair segment of the control region. The region comprises 201 pyrimidine sites and 159 purine sites ...
... mt DNA sequences from 63 individuals. The sample approximated a random sample of individuals in the tribe, to the extent to which this can be experimentally arranged. Each sequence is the first 360 basepair segment of the control region. The region comprises 201 pyrimidine sites and 159 purine sites ...
Physiological and Molecular Characterization of a Newly Identified
... for inundatory release against local insect pests because they have already adapted to the local climate and other population regulators [24] [5]. In addition, many countries are concerned about the introduction of exotic EPNs because they may have a negative impact on non-target organisms [5]. The ...
... for inundatory release against local insect pests because they have already adapted to the local climate and other population regulators [24] [5]. In addition, many countries are concerned about the introduction of exotic EPNs because they may have a negative impact on non-target organisms [5]. The ...
Risks from GMOs due to Horizontal Gene Transfer
... 2002; Davison, 1999; Lilley et al., 2003; van den Eede et al., 2004; Wolska, 2003). Some settings, such as bacterial biofilms, reveal highly efficient HGT (Molin and Tolker-Nielsen, 2003; van Elsas et al., 2003; Wuertz et al., 2004), whereas the simplified conditions in laboratory studies probably lac ...
... 2002; Davison, 1999; Lilley et al., 2003; van den Eede et al., 2004; Wolska, 2003). Some settings, such as bacterial biofilms, reveal highly efficient HGT (Molin and Tolker-Nielsen, 2003; van Elsas et al., 2003; Wuertz et al., 2004), whereas the simplified conditions in laboratory studies probably lac ...
PDF
... Despite the use of several Weissella (W.) strains for biotechnological and probiotic purposes, certain species of this genus were found to act as opportunistic pathogens, while strains of W. ceti were recognized to be pathogenic for farmed rainbow trout. Herein, we investigated the pathogenic potent ...
... Despite the use of several Weissella (W.) strains for biotechnological and probiotic purposes, certain species of this genus were found to act as opportunistic pathogens, while strains of W. ceti were recognized to be pathogenic for farmed rainbow trout. Herein, we investigated the pathogenic potent ...
all chromosomes recombine in meiosis F2 plants
... Finding a gene based on phenotype • 1. 100’s of DNA markers mapped onto each chromosome – high density linkage map. • 2. identify markers linked to trait of interest by recombination analysis • 3. Narrow region down to a manageable length of DNA – for cloning and sequence comparison • 4. Compare mu ...
... Finding a gene based on phenotype • 1. 100’s of DNA markers mapped onto each chromosome – high density linkage map. • 2. identify markers linked to trait of interest by recombination analysis • 3. Narrow region down to a manageable length of DNA – for cloning and sequence comparison • 4. Compare mu ...
Protection against microbiological corrosion and - SIM
... – By feeding themselves with compounds from the coating. – By locally changing the circumstances (parameters)on the coating. ...
... – By feeding themselves with compounds from the coating. – By locally changing the circumstances (parameters)on the coating. ...
Hybrid Plasmids Containing the Pyruvate
... strain were not significantly amplified suggesting that expression of the ace and lpd genes could be tightly coupled to metabolic need (despite a high copy number) or that the average plasmid copy number is itself not significantly greater than one. In the case of pGS5 the elevated lipoamide dehydro ...
... strain were not significantly amplified suggesting that expression of the ace and lpd genes could be tightly coupled to metabolic need (despite a high copy number) or that the average plasmid copy number is itself not significantly greater than one. In the case of pGS5 the elevated lipoamide dehydro ...
The Use of Cytochrome B Sequence Variation in Estimation of
... traditional classificationof the genus.Johnsonet al. (1988) find a groupcontainingmembers of the subgenera Vireo and Lanivireo. a lone taxon containing the species V. bellii and a division of the subgenusVireosylvainto a gilvusand an olivaceusgroup.To test theseresults independently, sequencedata fr ...
... traditional classificationof the genus.Johnsonet al. (1988) find a groupcontainingmembers of the subgenera Vireo and Lanivireo. a lone taxon containing the species V. bellii and a division of the subgenusVireosylvainto a gilvusand an olivaceusgroup.To test theseresults independently, sequencedata fr ...
Potential alteration of U37K paleothermometer due to selective
... tion of UK37 values and identification of any metabolites produced during the different incubation times. In order to monitor the degradation of alkenones by the different bacteria, sterile controls were prepared in the same way as the bacterial incubation flasks, but without adding the bacterial in ...
... tion of UK37 values and identification of any metabolites produced during the different incubation times. In order to monitor the degradation of alkenones by the different bacteria, sterile controls were prepared in the same way as the bacterial incubation flasks, but without adding the bacterial in ...
Evolution of a pseudo-control region in the mitochondrial genome of
... section between the /RNAG'" and /RNAPh'genes consisting of a 338 bp nonrepetitive section followed by a cluster of 23 tandem repeats with a unit length of 48 bp. Since this noncoding section seems to be the remnant of an ancestral duplicated control region, it was named pseudo-control region (YCR). ...
... section between the /RNAG'" and /RNAPh'genes consisting of a 338 bp nonrepetitive section followed by a cluster of 23 tandem repeats with a unit length of 48 bp. Since this noncoding section seems to be the remnant of an ancestral duplicated control region, it was named pseudo-control region (YCR). ...
Twin methodology in epigenetic studies
... genetic (50% on average) as well as environmental influences with rDZ=0.5A+C. The variance of each twin is due to their genetic makeup, shared environmental influences and residual factors unique to each twin, i.e. A+C+E. The structural equation modelling (SEM) can be applied to estimate the A, C an ...
... genetic (50% on average) as well as environmental influences with rDZ=0.5A+C. The variance of each twin is due to their genetic makeup, shared environmental influences and residual factors unique to each twin, i.e. A+C+E. The structural equation modelling (SEM) can be applied to estimate the A, C an ...
Comparative studies on molecular techniques for detecting
... membrane was washed with 1 mol/L NaCl and TE (pH 8.0) to remove inhibitor factors. DNA fixed membrane was employed for PCR. PCR Assay: PCR was performed by a programmable thermal controller (model PTC-150, MJ Inc.) and the DNA Amplification Reagent Kit (Biomedical Inc.) with Taq polymerase, 1 U per ...
... membrane was washed with 1 mol/L NaCl and TE (pH 8.0) to remove inhibitor factors. DNA fixed membrane was employed for PCR. PCR Assay: PCR was performed by a programmable thermal controller (model PTC-150, MJ Inc.) and the DNA Amplification Reagent Kit (Biomedical Inc.) with Taq polymerase, 1 U per ...
Theoretical and Applied Genetics
... effective method to control this disease in canola production. In particular, blackleg resistance is considered as one of the most important traits in the canola breeding programs of all seed companies in Canada, Europe and Australia. Mapping blackleg resistance genes and eventually cloning these ge ...
... effective method to control this disease in canola production. In particular, blackleg resistance is considered as one of the most important traits in the canola breeding programs of all seed companies in Canada, Europe and Australia. Mapping blackleg resistance genes and eventually cloning these ge ...
scope and historical developments in microbiology
... John Snow, a British physician, traced the source of cholera to the municipal water supply of London during an 1854 outbreak. He reasoned that by avoiding the contaminated water source, people could avoid the disease. Snow’s recommendations were adopted and the spread of disease was halted. Both Sem ...
... John Snow, a British physician, traced the source of cholera to the municipal water supply of London during an 1854 outbreak. He reasoned that by avoiding the contaminated water source, people could avoid the disease. Snow’s recommendations were adopted and the spread of disease was halted. Both Sem ...
Chapter 4. Studying DNA Learning outcomes 4.1. Enzymes for DNA
... results of a restriction digest are examined 5. Distinguish between blunt- and sticky-end ligation and explain how the efficiency of blunt-end ligation can be increased 6. Give details of the key features of plasmid cloning vectors and describe how these vectors are used in cloning experiments, usin ...
... results of a restriction digest are examined 5. Distinguish between blunt- and sticky-end ligation and explain how the efficiency of blunt-end ligation can be increased 6. Give details of the key features of plasmid cloning vectors and describe how these vectors are used in cloning experiments, usin ...
Analysis of flower pigmentation mutants generated by random
... originally identified in a polymorphic DNA fragment containing the dfrC gene (Gerats et al., 1990). To isolate mutants in which various steps in plant development were altered we conducted a self-pollination program of W138 plants. This yielded a collection of mutants with alterations in growth char ...
... originally identified in a polymorphic DNA fragment containing the dfrC gene (Gerats et al., 1990). To isolate mutants in which various steps in plant development were altered we conducted a self-pollination program of W138 plants. This yielded a collection of mutants with alterations in growth char ...
FX 8 - ACMG
... gene (FMR1) was performed by Triplet Repeat Primed PCR followed by capillary electrophoresis. Southern blot analysis, when performed, uses the StB12.3 probe and genomic DNA digested with EagI/EcoRI. Up to 44 copies of the CGG repeat within FMR-1 is considered normal. Premutation alleles are usually ...
... gene (FMR1) was performed by Triplet Repeat Primed PCR followed by capillary electrophoresis. Southern blot analysis, when performed, uses the StB12.3 probe and genomic DNA digested with EagI/EcoRI. Up to 44 copies of the CGG repeat within FMR-1 is considered normal. Premutation alleles are usually ...
Glencoe Biology
... Where the mutation is in the genome Type of protein that the mutation affects Population in which the mutation occurs ...
... Where the mutation is in the genome Type of protein that the mutation affects Population in which the mutation occurs ...
Chapter 12
... Chapter 12 - DNA Technology and the Human Genome How can we use bacteria to manipulate DNA and protein? Recall how a bacterium defends itself when a bacteriophage injects its DNA into a bacterium… The bacterium has enzymes called restriction enzymes that attempt to cut up the bacteriophage DNA befo ...
... Chapter 12 - DNA Technology and the Human Genome How can we use bacteria to manipulate DNA and protein? Recall how a bacterium defends itself when a bacteriophage injects its DNA into a bacterium… The bacterium has enzymes called restriction enzymes that attempt to cut up the bacteriophage DNA befo ...
The Differential Killing of Genes by Inversions in Prokaryotic Genomes
... from ftp://www.ncbi.nlm.nih.gov. The two pairs of genomes were chosen because, according to parameters of asymmetry in each of these genomes, the genes lying on the leading strand form a set distinct from the set of genes lying on the lagging strand. We have extracted amino acid sequences of ortholo ...
... from ftp://www.ncbi.nlm.nih.gov. The two pairs of genomes were chosen because, according to parameters of asymmetry in each of these genomes, the genes lying on the leading strand form a set distinct from the set of genes lying on the lagging strand. We have extracted amino acid sequences of ortholo ...
Chapter 14: Gene Transcription and RNA Modification
... This section outlines the process of transcription in bacteria. As was the case with replication, it is better to understand the simpler bacterial transcription system before proceeding to the more complex eukaryotic one. Before entering into the discussion of initiation, elongation, and termination ...
... This section outlines the process of transcription in bacteria. As was the case with replication, it is better to understand the simpler bacterial transcription system before proceeding to the more complex eukaryotic one. Before entering into the discussion of initiation, elongation, and termination ...
Infectious Bursal Disease Virus (IBDV) genesig
... The Negative Control should be completely free of any DNA/RNA. If you see this error message it means that at some point during the setup, the Negative Control has been contaminated with DNA/RNA and has given a positive signal. This contamination has invalidated the test. The Positive Control and yo ...
... The Negative Control should be completely free of any DNA/RNA. If you see this error message it means that at some point during the setup, the Negative Control has been contaminated with DNA/RNA and has given a positive signal. This contamination has invalidated the test. The Positive Control and yo ...
aquificae.2 - Pace University ePortfolio
... between 85-95 degrees Celsius. It uses oxygen respiration to survive, even though it can exist where oxygen is as low as 7.5 ppm. It also creates water as a byproduct. A.aeolicus’s genome has been sequenced and it was found that it is one third the length of a genome of E.coli. It has been found th ...
... between 85-95 degrees Celsius. It uses oxygen respiration to survive, even though it can exist where oxygen is as low as 7.5 ppm. It also creates water as a byproduct. A.aeolicus’s genome has been sequenced and it was found that it is one third the length of a genome of E.coli. It has been found th ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.