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Bacterial biofilms: Importance in animal diseases
Bacterial biofilms: Importance in animal diseases

... Antibiotic therapy usually eliminates bacteria in planktonic stage, but it cannot penetrate the biofilm. On the other hand, there have also been found hydrolytic enzymes of the β-lactamase type that are synthesized in small amounts but that are kept caught and concentrated in the matrix of the biofi ...
Whole Genome Sequencing versus Traditional Genotyping for
Whole Genome Sequencing versus Traditional Genotyping for

... approximately 50% of the resulting clusters could be confirmed by contact tracing documenting transmission links ([8], unpublished data). Among several reasons that might account for this, transmission can occur during short contacts or in high risk populations (e.g., homeless or alcoholic populatio ...
A Unified Statistical Framework for RNA Sequence Data from
A Unified Statistical Framework for RNA Sequence Data from

... e that are priorly known to be nonother words, there are several rows of A zero at only one column. This is equivalent to the separability assumption introduced by Donoho and Stodden (2003) for the uniqueness of NMF. Unfortunately, marker genes are rarely known in practice. In fact, extracting high- ...
Other RNA Processing Events
Other RNA Processing Events

... initial cleavages that separate the individual large rRNAs – RNase E is another ribonuclease that is responsible for removing the 5S rRNA from the precursor ...
BIOLOGY  SUPPORT   MATERIAL
BIOLOGY SUPPORT MATERIAL

... Ans: Bamboo species flower only once in their life-times generally after 50-100 years. 4- What is meant by homothallic? Ans: The term homothallic refers to bisexual or hermaphrodite condition. 5- Why are the date palms referred to as dioecious ? Ans: In date-palms, the male and female flowers are pr ...
Sequence Note Complete 59 Long  Terminal Repeat, nef,
Sequence Note Complete 59 Long Terminal Repeat, nef,

... strains, is found in our isolates. Notably, isolate TV005 does not have this subtype C-specific Rev truncation and thus has an Rev protein 126 amino acids in length. Isolates TV014 and TV019 have a seven-residue deletion directly following the nuclear export signal (NES), which is also present in on ...
General - Bioinformatics Research Group at SRI International
General - Bioinformatics Research Group at SRI International

... X = total number of reactions in P Y = enzymes catalyzing number of reactions for which there is evidence in O Z = number of Y reactions that are used in other pathways in O ...
Using the Wolbachia Bacterial Symbiont to Teach Inquiry
Using the Wolbachia Bacterial Symbiont to Teach Inquiry

... THE WOLBACHIA BACTERIAL SYMBIONT ...
Applied Environmental Microbiology
Applied Environmental Microbiology

... (36), age (37), and root lysates, secretions, or exudates (38) are determinants of root microbiotas. Factors that determine root exudates, such as availability of inorganic nutrients, temperature, light intensity, O2/CO2 level, or root damage, may indirectly affect root microbiotas (39). The presenc ...
Student`s guide -
Student`s guide -

... sequence that can be ‘recognised’ and cut by the restriction enzyme BamHI. In contrast, D has no restriction site and therefore it cannot be cut by the enzyme. Individuals who are homozygous for the dominant allele (DD) only have DNA of type D, which is not cut by the restriction enzyme. Gel electro ...
Natural genetic transformation: prevalence, mechanisms
Natural genetic transformation: prevalence, mechanisms

... subdivision) strongly prefer to take up DNA containing their own specific DNA uptake signal sequences (DUS or USS). Thus, it was shown more than twenty years ago that N. gonorrhoeae does not take up DNA from H. influenzae and viceversa [91]. Both the 12 bp neisserial DUS sequence and its 9e10 bp USS ...
FEMS Microbiology Letters
FEMS Microbiology Letters

... were characterised from A. variabilis and Anabaena 7119, respectively. Now an 1.1 kb nifJ probe, containing part of the earlier characterised 267 bp segment, was generated by inverse PCR from Anabaena 7119 and used in hybridisation experiments of EcoRV/HindIII-digested genomic DNA from A. variabilis ...
Release Notes for Genomes Processed Using Complete Genomics
Release Notes for Genomes Processed Using Complete Genomics

... Results from SV analysis are reported in the following four files: i. allJunctionsBeta-[ASM-ID].tsv file reports all junctions detected in the sequence genome, with associated information including genomic coordinates of breakpoints, number of discordant mate pairs supporting each junction, assemble ...
(base) sequence of the genome might reflect biological information
(base) sequence of the genome might reflect biological information

... of chromosome II was enlarged to 990 nt (Figure 2(c), 648 ~ 1,638), or 1,584 nt (Figure 2(d), 54 ~ 1,638), respectively. That is, the base sequence of the complete ATP1 gene had self-similarity to the gene-position on chromosome II. Other genes of S. cerevisiae were highly homologous with the gene-p ...
RNA-Mediated Programming of Developmental
RNA-Mediated Programming of Developmental

... the vector sequence. Minichromosomes were obtained by inserting 165 bp of natural Paramecium telomeric repeats, ending with a synthetic BamHI site, into both the SalI and XhoI sites. After BamHI digestion, the minichromosomes were purified from the vector by agarose gel electrophoresis. The frameshi ...
Simple Sequence Repeats as Advantageous Mutators
Simple Sequence Repeats as Advantageous Mutators

... Runx-2, and yeast RAS2. Ideally, an incremental effect of repeat number should be demonstrated over a range of quantitative phenotypic differences. Although a few studies have provided data from multiple alleles [e.g., 4, 17, 32], and the triplet repeat diseases also show dependence on repeat number ...
Divergent evolution of oxidosqualene cyclases in plants
Divergent evolution of oxidosqualene cyclases in plants

... & Huelsenbeck, 2003) was used, with 10 000 000 generations performed with a sampling every 10 000 generations by the Markov chain Monte Carlo method. For molecular evolution analysis, genes from the CS and LS groups were separated and used to construct CS-derived and LSderived phylogenetic trees, re ...
The nucleotide sequence of Saccharomyces cerevisiae
The nucleotide sequence of Saccharomyces cerevisiae

... and TRP5 markers had been built, allowing sequencing and transcriptional mapping of nine ORFs located in the 24-kb region spanning the PMA1 and ATE1 loci. Analysis of these data led to the estimation of a minimum number of 5,300 expressed genes in yeast. In this centromeric region a recombination fr ...
Sorting Out the Genome
Sorting Out the Genome

... a mathematical point of view, however, the pancake problem remains unsolved: The exact number of flips needed to sort n pancakes is unknown. Gene flipping has a lot in common with the pancake problem, but there are differences as well. With pancakes, you are not allowed to reach into the middle of t ...
Divergent evolution of oxidosqualene cyclases in plants
Divergent evolution of oxidosqualene cyclases in plants

... & Huelsenbeck, 2003) was used, with 10 000 000 generations performed with a sampling every 10 000 generations by the Markov chain Monte Carlo method. For molecular evolution analysis, genes from the CS and LS groups were separated and used to construct CS-derived and LSderived phylogenetic trees, re ...
The Genome of a Mongolian Individual Reveals
The Genome of a Mongolian Individual Reveals

... Consortium 2010, 2012) a reality and facilitated genomebased, personal medicine. Representative genomes of increasing numbers of human populations have been sequenced to dissect the structure and history, including Indian (Reich et al. 2009), American (Reich et al. 2012), and Jewish (Behar et al. 20 ...
The role of variable DNA tandem repeats in bacterial adaptation
The role of variable DNA tandem repeats in bacterial adaptation

... DNA tandem repeats (TRs), also designated as satellite DNA, are inter- or intragenic nucleotide sequences that are repeated two or more times in a headto-tail manner. Because TR tracts are prone to strand-slippage replication and recombination events that cause the TR copy number to increase or decr ...
New Phytologist Supporting Information Notes S1  Populus trichocarpa
New Phytologist Supporting Information Notes S1 Populus trichocarpa

... expression values were estimated using the latest publically available genome sequences and annotated ...
Woolfe, 2005
Woolfe, 2005

... Tissues from few cells are underrepresented Late developing tissues or cell types after 24 h will be missed completely ...
Rooting the Universal Tree of Life Using Genomic Fusion
Rooting the Universal Tree of Life Using Genomic Fusion

... (Buc), will be used as the root of Enterobacteriales branch. This strategy, similar as the neighbor-join method, can be recursively used to find the root of Gammaproteobacteria which again is Buc. The corresponding rooted Gammaproteobacteria tree is drawn at the right-bottom in Fig. 3. It is worthy ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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