A helicoidal transfer matrix model for inhomogeneous DNA melting
... the discrete helix-coil models. Unlike the helix-coil models, which have seen many applications to real biological sequences, the particle-lattice models are mostly used to obtain a more fundamental, sequence independent, physical understanding of the DNA melting phenomenon, such as the order of the ...
... the discrete helix-coil models. Unlike the helix-coil models, which have seen many applications to real biological sequences, the particle-lattice models are mostly used to obtain a more fundamental, sequence independent, physical understanding of the DNA melting phenomenon, such as the order of the ...
Identification and Analysis of Arabidopsis Expressed Sequence
... a family of ncRNAs with members in several plant species (Taylor and Green, 1995; Teramoto et al., 1996; van Hoof et al., 1997). GUT15 (gene with unstable transcript 15) is another characterized member of this family. This transcript was first identified as one of the most unstable transcripts in to ...
... a family of ncRNAs with members in several plant species (Taylor and Green, 1995; Teramoto et al., 1996; van Hoof et al., 1997). GUT15 (gene with unstable transcript 15) is another characterized member of this family. This transcript was first identified as one of the most unstable transcripts in to ...
The Evolutionary History of Human and Chimpanzee Y
... although there are no lineage-specific gene-disruptive mutations in the X-degenerate portion of the Y-chromosome fixed within humans, surprisingly, 4 genes, CYorf15B, TBL1Y, TMSB4Y, and USP9Y, are disrupted by one or more splice site or premature stop codon mutations in chimpanzees (Hughes et al. 20 ...
... although there are no lineage-specific gene-disruptive mutations in the X-degenerate portion of the Y-chromosome fixed within humans, surprisingly, 4 genes, CYorf15B, TBL1Y, TMSB4Y, and USP9Y, are disrupted by one or more splice site or premature stop codon mutations in chimpanzees (Hughes et al. 20 ...
Nomenclature I
... while following the rules described previously, e.g., ACH for “achondroplasia.” It is usual for this symbol to change when the gene product or function is identified; however, if there is no additional information derived from the cloned gene, the disease symbol, e.g., ACH, will be maintained. If an ...
... while following the rules described previously, e.g., ACH for “achondroplasia.” It is usual for this symbol to change when the gene product or function is identified; however, if there is no additional information derived from the cloned gene, the disease symbol, e.g., ACH, will be maintained. If an ...
MOTIFS MOTIFSMARTIFAMORIFSMOOTIFSMICIFC
... • A microarray experiment showed that when gene X is knocked out, 20 other genes are not expressed – How can one gene have such drastic effects? ...
... • A microarray experiment showed that when gene X is knocked out, 20 other genes are not expressed – How can one gene have such drastic effects? ...
Lecture 3: Sequence Alignment
... Compare the gene eyeless of Drosophila Melanoganster with the human gene aniridia. They are master regulatory genes producing proteins that control large cascade of other genes. Certain segments of genes eyeless of Drosophila melanogaster and human aniridia are almost identical. The most important o ...
... Compare the gene eyeless of Drosophila Melanoganster with the human gene aniridia. They are master regulatory genes producing proteins that control large cascade of other genes. Certain segments of genes eyeless of Drosophila melanogaster and human aniridia are almost identical. The most important o ...
Bacteriophage functional genomics and its role in
... uses BLASTP matches to a database, but chooses regions based on probability statistics rather than heuristics. PHAST [55] is a web server that also uses homology to identify prophage regions, but is unique in that it does not require an annotated genome as input. Prophages have also been identified ...
... uses BLASTP matches to a database, but chooses regions based on probability statistics rather than heuristics. PHAST [55] is a web server that also uses homology to identify prophage regions, but is unique in that it does not require an annotated genome as input. Prophages have also been identified ...
Recurrent detection of VIM-1- producing
... only sporadically reported from non-human sources.2,3 In Europe, carbapenems are not licensed for veterinary use and no maximum residue levels are defined. Therefore, the isolation of VIM-1producing Escherichia coli and Salmonella spp. in 2011–12 from German swine and poultry farms raised concerns t ...
... only sporadically reported from non-human sources.2,3 In Europe, carbapenems are not licensed for veterinary use and no maximum residue levels are defined. Therefore, the isolation of VIM-1producing Escherichia coli and Salmonella spp. in 2011–12 from German swine and poultry farms raised concerns t ...
Evolution of genetic and genomic features unique to the human
... currently available. As these two genome sequences are far less accurate and complete than the human assembly, this Review does not rely on them unless the ancient hominin sequencing data for a particular sequence is of a high quality. Our working definition of HLS therefore requires that changes fo ...
... currently available. As these two genome sequences are far less accurate and complete than the human assembly, this Review does not rely on them unless the ancient hominin sequencing data for a particular sequence is of a high quality. Our working definition of HLS therefore requires that changes fo ...
Restriction Enzymes in Microbiology, Biotechnology and
... R-M system. This grows efficiently in the absence of an R-M system (middle row, left; eop=1) because no restriction enzyme is present, and also in the presence of PstI (middle row, middle; eop=1) because the phage DNA carries the protective, PstIspecific, modification. It grows poorly, however, on t ...
... R-M system. This grows efficiently in the absence of an R-M system (middle row, left; eop=1) because no restriction enzyme is present, and also in the presence of PstI (middle row, middle; eop=1) because the phage DNA carries the protective, PstIspecific, modification. It grows poorly, however, on t ...
Comparison of three molecular methods for typing Aeromonas
... For instance, an identical ISR-RFLP type was obtained for isolates 203, 210 and 137 (Figure 1), while they showed specific patterns with the other two methods (Figures 2, 3). This may be considered a shortcoming of the ISR-RFLP method and additionally, as already commented, it is more complex to perf ...
... For instance, an identical ISR-RFLP type was obtained for isolates 203, 210 and 137 (Figure 1), while they showed specific patterns with the other two methods (Figures 2, 3). This may be considered a shortcoming of the ISR-RFLP method and additionally, as already commented, it is more complex to perf ...
Mutation Analysis of the Ornithine Transcarbamylase (OTC) Gene in
... two known and three novel mutations of the OTC gene in five Japanese patients including two neonatal-onset, one late-onset, and two symptomatic female patients. Known nonsense mutations (c.578G>A and c.421C>T) were detected in a neonatal-onset male and a symptomatic female patient, respectively. Mut ...
... two known and three novel mutations of the OTC gene in five Japanese patients including two neonatal-onset, one late-onset, and two symptomatic female patients. Known nonsense mutations (c.578G>A and c.421C>T) were detected in a neonatal-onset male and a symptomatic female patient, respectively. Mut ...
A new ultrasonic signal amplification method for detection of bacteria
... temperature inside an ultra violet (UV) flow chamber. The test was performed as follows. 1 ml of the agar medium is mixed with 1 ml of the sample and analyzed in the 3 ml plastic cuvette. Then 100 /xl of hydrogen peroxide solution 10 M in distilled water is added and shaken three times vigorously to ...
... temperature inside an ultra violet (UV) flow chamber. The test was performed as follows. 1 ml of the agar medium is mixed with 1 ml of the sample and analyzed in the 3 ml plastic cuvette. Then 100 /xl of hydrogen peroxide solution 10 M in distilled water is added and shaken three times vigorously to ...
FastGene Taq DNA Polymerase
... assays. For GC-rich targets (>65% GC content) 5 min at 95 °C may be used. ...
... assays. For GC-rich targets (>65% GC content) 5 min at 95 °C may be used. ...
The use of amplified fragment length polymorphism (AFLP) in the
... exists (ÔYÕ refers to Y or W chromosomes unless stated otherwise), much of the DNA on this chromosome is not unique. This is because copies of mobile genetic elements, repeat sequences and a pseudoautosomal region can occur elsewhere in the genome (Charlesworth 1991). Accordingly, sex-linked markers ...
... exists (ÔYÕ refers to Y or W chromosomes unless stated otherwise), much of the DNA on this chromosome is not unique. This is because copies of mobile genetic elements, repeat sequences and a pseudoautosomal region can occur elsewhere in the genome (Charlesworth 1991). Accordingly, sex-linked markers ...
Tools for genetic analysis in Trypanosoma brucei unlinked fields
... Strategies for regulated gene expression and conditional gene knockouts Cell lines for regulated expression Regulated gene expression has prerequisites: it has to be done in a transgenic cell line that expresses the Tet repressor and, depending on the vector, the T7 RNA polymerase. For these reasons ...
... Strategies for regulated gene expression and conditional gene knockouts Cell lines for regulated expression Regulated gene expression has prerequisites: it has to be done in a transgenic cell line that expresses the Tet repressor and, depending on the vector, the T7 RNA polymerase. For these reasons ...
http://www.life.umd.edu/grad/mlfsc/ DNA Bracelets
... The coded amino acids were changed significantly. 3) What happens to the amino acid chain if the frame shift results in an RNA codon of UAA, UAG, or UGA? These are termination codons. Translation will stop prematurely. 4) How will the changes in amino acids affect the protein that is expressed by th ...
... The coded amino acids were changed significantly. 3) What happens to the amino acid chain if the frame shift results in an RNA codon of UAA, UAG, or UGA? These are termination codons. Translation will stop prematurely. 4) How will the changes in amino acids affect the protein that is expressed by th ...
Molecular Determinants of Alphavirus Neurovirulence: Nucleotide
... mpl0 and mpl 1. The entire sequence was determined on each strand of the pTC-5 c D N A and all the junctions between restriction fragments were confirmed by sequencing other fragments which contained these junctions in an internal position. In the region encoding the capsid, it was necessary to buil ...
... mpl0 and mpl 1. The entire sequence was determined on each strand of the pTC-5 c D N A and all the junctions between restriction fragments were confirmed by sequencing other fragments which contained these junctions in an internal position. In the region encoding the capsid, it was necessary to buil ...
poster_CSHL_2007
... • Importance of RNA motifs in shaping transcriptomes (~30% of yeast, worm, human, arabidopsis motifs we found are RNA motifs) ...
... • Importance of RNA motifs in shaping transcriptomes (~30% of yeast, worm, human, arabidopsis motifs we found are RNA motifs) ...
PowerPoint File, 13.82 MB
... • ~2,000 expressed genes present in teosinte are missing from the B73 genome. 72 of these genes are missing from all other tested maize lines. • Teosinte genes failed to pass through the domestication bottleneck for a variety of reasons (selection for or against haplotypes and random processes). • T ...
... • ~2,000 expressed genes present in teosinte are missing from the B73 genome. 72 of these genes are missing from all other tested maize lines. • Teosinte genes failed to pass through the domestication bottleneck for a variety of reasons (selection for or against haplotypes and random processes). • T ...
An Experimental Program for Introducing First
... the students to analyze the data. The groups also received a general packet which clarified any unknown terminology or figures. The second assignment held a greater amount of relative credit (250% in comparison to assignment 1). It was expected that the students would be more capable when it came to ...
... the students to analyze the data. The groups also received a general packet which clarified any unknown terminology or figures. The second assignment held a greater amount of relative credit (250% in comparison to assignment 1). It was expected that the students would be more capable when it came to ...
Ribosomal frameshifting in decoding antizyme mRNAs from yeast
... years. As pointed out previously we have been unable to amplify it from human genomic DNA (14). Based on these and other considerations, we now believe that this cDNA is a contaminant, most likely a mammalian antizyme 1 gene belonging to an unidentified rabbit or hare species. In our search for new a ...
... years. As pointed out previously we have been unable to amplify it from human genomic DNA (14). Based on these and other considerations, we now believe that this cDNA is a contaminant, most likely a mammalian antizyme 1 gene belonging to an unidentified rabbit or hare species. In our search for new a ...
Kreitman review on positive selection
... Statistical tests can capitalize on the multiple time depths provided by withinand between-species variation, and some tests use both kinds of information. This review of methods to detect selection, accordingly, does not restrict itself to only one arena. The strongest inferences about selection ca ...
... Statistical tests can capitalize on the multiple time depths provided by withinand between-species variation, and some tests use both kinds of information. This review of methods to detect selection, accordingly, does not restrict itself to only one arena. The strongest inferences about selection ca ...
Bewildering Bs: an impression of the 1st B-Chromosome
... Bs? This does not seem to result solely from a lack of study. From a selective point of view, it may be that there is a narrow niche for a B to exist in a species and hence strong selection for a particular type of B. Alternatively, because Bs differ in their effects and transmission rates, the 'bes ...
... Bs? This does not seem to result solely from a lack of study. From a selective point of view, it may be that there is a narrow niche for a B to exist in a species and hence strong selection for a particular type of B. Alternatively, because Bs differ in their effects and transmission rates, the 'bes ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.