Feature Article - American Society for Microbiology
... a disease, it must be (i) consistently found in association with the disease, (ii) isolated from the disease, and (iii) able to produce the disease in its isolated state . These precepts, which came to be more commonly known as Koch’s postulates because of the definitive way in which he used them in ...
... a disease, it must be (i) consistently found in association with the disease, (ii) isolated from the disease, and (iii) able to produce the disease in its isolated state . These precepts, which came to be more commonly known as Koch’s postulates because of the definitive way in which he used them in ...
Handout - 6 - CM - Outline Genomic Model for HL7 V2
... the regions in narrative. E.g. “all coding regions and appropriate flanking regions”. This variable is included to capture such descriptions. It is only relevant to sequencing studies. May or may not be needed depending on the Reference sequences to which the results are anchored. It is needed for w ...
... the regions in narrative. E.g. “all coding regions and appropriate flanking regions”. This variable is included to capture such descriptions. It is only relevant to sequencing studies. May or may not be needed depending on the Reference sequences to which the results are anchored. It is needed for w ...
Introduction slides on BASE
... Open MS Excel and tell it to open the file you downloaded (typically called base.tsv). ...
... Open MS Excel and tell it to open the file you downloaded (typically called base.tsv). ...
A simplified subtractive hybridization protocol used to isolate DNA
... et al. (1985) was used to isolate DNA sequences specific to Xylella fastidiosa isolated from diseased citrus plants. Xy. fastidiosa is a unique species in the genus and consists of several pathotypes causing diseases in economically important plants, including alfalfa, almond, grapevine, peach and p ...
... et al. (1985) was used to isolate DNA sequences specific to Xylella fastidiosa isolated from diseased citrus plants. Xy. fastidiosa is a unique species in the genus and consists of several pathotypes causing diseases in economically important plants, including alfalfa, almond, grapevine, peach and p ...
Bacteria Transformation
... human insulin. The technology which made this approach possible was the development of recombinant DNA techniques. In simple terms, the human gene which codes for the insulin protein was cloned (copied) and then put inside of bacteria. A number of tricks were performed on this gene to make the bacte ...
... human insulin. The technology which made this approach possible was the development of recombinant DNA techniques. In simple terms, the human gene which codes for the insulin protein was cloned (copied) and then put inside of bacteria. A number of tricks were performed on this gene to make the bacte ...
Analysis of a Rhizobium leguminosarum gene
... approximately 5 kb upstream of the previously identified feuP gene (Yeoman et al., 1997). This fragment was cloned into pUC18 to form pBI0860. Various restriction fragments from pBI0860 were subcloned into pUC18 and their sequences were determined. The sequence of the entire 3.7 kb fragment was then ...
... approximately 5 kb upstream of the previously identified feuP gene (Yeoman et al., 1997). This fragment was cloned into pUC18 to form pBI0860. Various restriction fragments from pBI0860 were subcloned into pUC18 and their sequences were determined. The sequence of the entire 3.7 kb fragment was then ...
Intelligence: Genetics, Genes, and Genomics
... More is known about the genetics of intelligence than about any other trait, behavioral or biological, which is selectively reviewed in this article. Two of the most interesting genetic findings are that heritability of intelligence increases throughout the life span and that the same genes affect d ...
... More is known about the genetics of intelligence than about any other trait, behavioral or biological, which is selectively reviewed in this article. Two of the most interesting genetic findings are that heritability of intelligence increases throughout the life span and that the same genes affect d ...
"PCR-based Species Identification and Gene Tagging"
... maize leaf _Bots infected leaf pt3 infected leaf pt1 P sorghi pt3 P sorghi pt1 Marker ...
... maize leaf _Bots infected leaf pt3 infected leaf pt1 P sorghi pt3 P sorghi pt1 Marker ...
Molecular Testing and Clinical Diagnosis
... • DNA fragments (probes) are anchored to a glass or silicon chip. • DNA probes within the microarray can measure the gene expression of thousands of genes in a single RNA sample • Click on link for information sheet National Institutes of Health – Information Sheet ...
... • DNA fragments (probes) are anchored to a glass or silicon chip. • DNA probes within the microarray can measure the gene expression of thousands of genes in a single RNA sample • Click on link for information sheet National Institutes of Health – Information Sheet ...
Document
... plant’s G5K domain have a vital role in functional confirmation. The results of our study are also in conformity with this, as the β4-αE loop of G5K is found to be conserved throught the plants selected for the study. " ->" They also found that aspartic acid (D148) of the β4-αE loop in the G5K domai ...
... plant’s G5K domain have a vital role in functional confirmation. The results of our study are also in conformity with this, as the β4-αE loop of G5K is found to be conserved throught the plants selected for the study. " ->" They also found that aspartic acid (D148) of the β4-αE loop in the G5K domai ...
Ascorbate peroxidaserelated (APxR) is a new
... Amoebozoa, and animals. APx and CcP are closely related. However, APx sequences are found in chloroplast-containing organisms, whereas CcP genes are found in most mitochondria-containing organisms. Except for the land plants, which contain only APx, chloroplastic organisms contain both APx and CcP s ...
... Amoebozoa, and animals. APx and CcP are closely related. However, APx sequences are found in chloroplast-containing organisms, whereas CcP genes are found in most mitochondria-containing organisms. Except for the land plants, which contain only APx, chloroplastic organisms contain both APx and CcP s ...
Systematic and Applied Microbiology
... 2006 from rice and 7 of the 131 clinical isolates (5.3% of the total) did not ferment adonitol. All adonitol negative strains were selected (Table 2). One adonitol positive clinical strain (910) was also included in the sequencing assays, as well as strain B5R5 [31], an Enterobacter banana isolate. ...
... 2006 from rice and 7 of the 131 clinical isolates (5.3% of the total) did not ferment adonitol. All adonitol negative strains were selected (Table 2). One adonitol positive clinical strain (910) was also included in the sequencing assays, as well as strain B5R5 [31], an Enterobacter banana isolate. ...
Chapter 10 - McGraw Hill Higher Education
... 10.2 The Human Genome The sequence of the entire human genome was reported on June 26, 2000 It consists of 3.2 billion base pairs If the human genome were a book It would be 500,000 pages long It would take about 60 years to read at the rate of 8 hours a day, every day, at five bases a second ...
... 10.2 The Human Genome The sequence of the entire human genome was reported on June 26, 2000 It consists of 3.2 billion base pairs If the human genome were a book It would be 500,000 pages long It would take about 60 years to read at the rate of 8 hours a day, every day, at five bases a second ...
The linear chromosome of the plant
... has evolved from Gram-positive bacteria [3]. Mycoplasmas are among the smallest self-replicating organisms known, and are characterized by a small genome with a low G+C content, and a lack of a firm cell wall. The genome sizes of phytoplasmas are estimated to range from 0.53 to 1.35 Mb [4] with a GC ...
... has evolved from Gram-positive bacteria [3]. Mycoplasmas are among the smallest self-replicating organisms known, and are characterized by a small genome with a low G+C content, and a lack of a firm cell wall. The genome sizes of phytoplasmas are estimated to range from 0.53 to 1.35 Mb [4] with a GC ...
Evolution of Gene Order and Chromosome Number in
... eye, to permit inclusion of some very short but highly conserved sequence matches that occurred at the ends of database sequences. The S. pombe dataset comprised 1·577 megabases (55 cosmids in 27 contigs) from chromosome I sequenced at the Sanger Centre, and a consistent significance threshold (BLAS ...
... eye, to permit inclusion of some very short but highly conserved sequence matches that occurred at the ends of database sequences. The S. pombe dataset comprised 1·577 megabases (55 cosmids in 27 contigs) from chromosome I sequenced at the Sanger Centre, and a consistent significance threshold (BLAS ...
Full-Text PDF
... identification of UCEs. These constraints are now being addressed with fast and efficient solutions with no constraints on the underlying models [18,19]. These approaches have led us to an unexpected result: at least for some organelles and taxa, the genome structure and HCE set, despite themselves ...
... identification of UCEs. These constraints are now being addressed with fast and efficient solutions with no constraints on the underlying models [18,19]. These approaches have led us to an unexpected result: at least for some organelles and taxa, the genome structure and HCE set, despite themselves ...
Detection of Borrelia burgdorferi in cerebrospinal fluid by the
... from patients and in ticks. However, culture of this fastidious organism is difficult and requires incubation for about 6 weeks.'79l g 9*' We have established PCR for rapid and sensitive detection of B. burgdorferi in CSF. Because clinical samples contain only a few organisms, sensitivity is an obli ...
... from patients and in ticks. However, culture of this fastidious organism is difficult and requires incubation for about 6 weeks.'79l g 9*' We have established PCR for rapid and sensitive detection of B. burgdorferi in CSF. Because clinical samples contain only a few organisms, sensitivity is an obli ...
Detection of Borrelia burgdorferi in cerebrospinal fluid by the
... from patients and in ticks. However, culture of this fastidious organism is difficult and requires incubation for about 6 weeks.'79l g 9*' We have established PCR for rapid and sensitive detection of B. burgdorferi in CSF. Because clinical samples contain only a few organisms, sensitivity is an obli ...
... from patients and in ticks. However, culture of this fastidious organism is difficult and requires incubation for about 6 weeks.'79l g 9*' We have established PCR for rapid and sensitive detection of B. burgdorferi in CSF. Because clinical samples contain only a few organisms, sensitivity is an obli ...
"Dual-coding Regions in Alternatively Spliced Human Genes". In
... region (Liang and Landweber, 2006). Furthermore, this number is probably an underestimate, because not all spliced versions of every alternatively spliced gene are available. More recently, Tress and colleagues examined alternatively spliced patterns in the ENCODE project comprising close examinatio ...
... region (Liang and Landweber, 2006). Furthermore, this number is probably an underestimate, because not all spliced versions of every alternatively spliced gene are available. More recently, Tress and colleagues examined alternatively spliced patterns in the ENCODE project comprising close examinatio ...
Analyzer Manual - immunoSEQ Analyzer
... By default, the “Summary” project will be selected. “Summary” is the full list of all your available samples. To deselect “Summary,” simply click on it. To choose a different project, click on its name. You may select multiple projects at a time and all samples in the selected projects will appear i ...
... By default, the “Summary” project will be selected. “Summary” is the full list of all your available samples. To deselect “Summary,” simply click on it. To choose a different project, click on its name. You may select multiple projects at a time and all samples in the selected projects will appear i ...
Integrated Analysis of Microarray Data and Gene Function Information.
... of genes simultaneously. Huge amount of genomewide gene expression data have been generated using microarrays. However, microarray data by themselves tell us very little about the underlying biological processes. On the other hand, a lot of biological knowledge have been obtained by conventional bio ...
... of genes simultaneously. Huge amount of genomewide gene expression data have been generated using microarrays. However, microarray data by themselves tell us very little about the underlying biological processes. On the other hand, a lot of biological knowledge have been obtained by conventional bio ...
View Full PDF - Biochemical Society Transactions
... Root- and stem-nodule bacteria collectively called rhizobia are capable of fixing atmospheric nitrogen in association with leguminous plants. In this process, a large amount of hydrogen is released as an obligate by-product of the nitrogen reduction. This hydrogen production has been described as on ...
... Root- and stem-nodule bacteria collectively called rhizobia are capable of fixing atmospheric nitrogen in association with leguminous plants. In this process, a large amount of hydrogen is released as an obligate by-product of the nitrogen reduction. This hydrogen production has been described as on ...
Data Availability and NASC tools
... • Data mining tool for finding interesting experiments for genes of interest. • It shows experiments where genes chosen are highly variable, over all the experiments in the database. • Shows if genes are correlated in any way. • It also allows you to pick slides that are different from the general t ...
... • Data mining tool for finding interesting experiments for genes of interest. • It shows experiments where genes chosen are highly variable, over all the experiments in the database. • Shows if genes are correlated in any way. • It also allows you to pick slides that are different from the general t ...
Identification and isolation of active N2O reducers in rice paddy soil
... for N2O reduction. For the stable isotope probing (SIP) experiment, 13C-labeled succinate was used to identify succinate-assimilating microbes under N2O-reducing conditions. DNA was extracted 24 h after incubation, and heavy and light DNA fractions were separated by density gradient ultracentrifugat ...
... for N2O reduction. For the stable isotope probing (SIP) experiment, 13C-labeled succinate was used to identify succinate-assimilating microbes under N2O-reducing conditions. DNA was extracted 24 h after incubation, and heavy and light DNA fractions were separated by density gradient ultracentrifugat ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.