The in vitro and in vivo effects of constitutive light - E
... aldehyde substrate from the fatty acid produced by the reaction. A significant advantage of the bacterial bioluminescence system is the ability to express the biosynthetic enzymes for substrate synthesis, allowing light to be produced constitutively. One of the underlying motivations for using lux-t ...
... aldehyde substrate from the fatty acid produced by the reaction. A significant advantage of the bacterial bioluminescence system is the ability to express the biosynthetic enzymes for substrate synthesis, allowing light to be produced constitutively. One of the underlying motivations for using lux-t ...
Recurrent patterns of DNA copy number alterations in tumors reflect
... The authors have performed somatic copy number alteration (CNA) profiling of human tumors across different tumor types, pursuing a pan-cancer analysis. Their main finding presented in this manuscript is that different cancers exhibit similarity in their copy-number alteration profiles when evaluated ...
... The authors have performed somatic copy number alteration (CNA) profiling of human tumors across different tumor types, pursuing a pan-cancer analysis. Their main finding presented in this manuscript is that different cancers exhibit similarity in their copy-number alteration profiles when evaluated ...
Annual Meeting of the Missouri Valley Branch of the American
... Due to the anatomical or functional loss of intestine, in majority of cases, intestinal failure might be occurred, in which the organ does not have its secretory and absorptive functions. Three-dimensional (3-D) porous scaffolds can play a vital role, as they not only serve as temporary templates to ...
... Due to the anatomical or functional loss of intestine, in majority of cases, intestinal failure might be occurred, in which the organ does not have its secretory and absorptive functions. Three-dimensional (3-D) porous scaffolds can play a vital role, as they not only serve as temporary templates to ...
hybrid DNA molecules
... of the cloned his3 gene that delete DNA sequences near or in the structural gene have been isolated and physically defined (unpublished data). The demonstration by Hinnen et al. (5) that recombinant DNA containing cloned yeast genes can be used to transform yeast cells clearly expands the potential ...
... of the cloned his3 gene that delete DNA sequences near or in the structural gene have been isolated and physically defined (unpublished data). The demonstration by Hinnen et al. (5) that recombinant DNA containing cloned yeast genes can be used to transform yeast cells clearly expands the potential ...
homolog of the agouti gene
... acceptor sites, except for the last region which, as expected, is flanked only by a splice acceptor at its 5' end. A polypyrimidine tract and putative branch point signals 10-50 bp upstream of each conserved 3' splice acceptor site were also identified (data not shown). Most notably, the ORF defined ...
... acceptor sites, except for the last region which, as expected, is flanked only by a splice acceptor at its 5' end. A polypyrimidine tract and putative branch point signals 10-50 bp upstream of each conserved 3' splice acceptor site were also identified (data not shown). Most notably, the ORF defined ...
Phylogenetic Relationships between the Western Aster Yellows
... rRNA gene is a particularly valuable evolutionary marker because it is larger and its rRNA has a more complex secondary structure than the 5s rRNA gene. Because of its central role in cell metabolism, the prokaryote 16s rRNA gene is highly conserved across widely divergent taxa, which frequently exh ...
... rRNA gene is a particularly valuable evolutionary marker because it is larger and its rRNA has a more complex secondary structure than the 5s rRNA gene. Because of its central role in cell metabolism, the prokaryote 16s rRNA gene is highly conserved across widely divergent taxa, which frequently exh ...
Widespread expression of the bovine Agouti gene results from at
... In this paper, we report for the first time the structure and widespread expression of the bovine Agouti gene. Its pattern of expression results from at least three promoters. We also report potential regulatory sequences for pig and human ASIP gene as revealed by a comparative genomic analysis. Alt ...
... In this paper, we report for the first time the structure and widespread expression of the bovine Agouti gene. Its pattern of expression results from at least three promoters. We also report potential regulatory sequences for pig and human ASIP gene as revealed by a comparative genomic analysis. Alt ...
glycan associated protein of Legionella (PpiA)
... prepared in rabbits using the E. coli K-12 strain expressing the 19 kD protein. For Western blot analysis the antiserumwas adsorbed with the E. coli strain carrying only the vector (see Ludwig et a/. 13 ). SDS-PAGE and Western blot analysis. SOS-PAGE was performed according to Laemmli 27 using 10% p ...
... prepared in rabbits using the E. coli K-12 strain expressing the 19 kD protein. For Western blot analysis the antiserumwas adsorbed with the E. coli strain carrying only the vector (see Ludwig et a/. 13 ). SDS-PAGE and Western blot analysis. SOS-PAGE was performed according to Laemmli 27 using 10% p ...
2. Primer Design
... Consider the amino acids you will be adding to the protein of interest with this primer. In order to shift the reading frame appropriately, you will often have to add a few bases to the restriction sites in your primer. Also, adding the Kozak consensus above will often require You should figure out ...
... Consider the amino acids you will be adding to the protein of interest with this primer. In order to shift the reading frame appropriately, you will often have to add a few bases to the restriction sites in your primer. Also, adding the Kozak consensus above will often require You should figure out ...
Article The Landscape of Realized Homologous
... populations (Castillo-Ramirez et al. 2012; Joseph et al. 2012; Shapiro et al. 2012; Croucher et al. 2013). Most commonly, recombination in bacteria involves the replacement of DNA in the recipient genome with homologous sequence from a donor. However, the intensity of this gene-conversion-like proce ...
... populations (Castillo-Ramirez et al. 2012; Joseph et al. 2012; Shapiro et al. 2012; Croucher et al. 2013). Most commonly, recombination in bacteria involves the replacement of DNA in the recipient genome with homologous sequence from a donor. However, the intensity of this gene-conversion-like proce ...
Copy number variants and genetic traits: closer to the resolution of
... to several megabases of DNA per event, adding up to a significant fraction of the genome57–59. The discovery of extensive copy number variation in the genomes of normal individuals provides new hypotheses to account for the phenotypic variability among inherited (Mendelian and polygenic) disorders a ...
... to several megabases of DNA per event, adding up to a significant fraction of the genome57–59. The discovery of extensive copy number variation in the genomes of normal individuals provides new hypotheses to account for the phenotypic variability among inherited (Mendelian and polygenic) disorders a ...
Candidate gene analysis of thyroid hormone receptors
... that ¯ank a major-eect locus for metamorphic failure in A. mexicanum (Voss and Shaer, 1997). If TRa or TRb are linked to these ¯anking DNA markers, this would be consistent with a causal relationship between TRs and metamorphic failure that could be investigated in subsequent studies of gene struc ...
... that ¯ank a major-eect locus for metamorphic failure in A. mexicanum (Voss and Shaer, 1997). If TRa or TRb are linked to these ¯anking DNA markers, this would be consistent with a causal relationship between TRs and metamorphic failure that could be investigated in subsequent studies of gene struc ...
Document
... In this exercise, we will utilize the UCSC Genome Browser to view a refined whole genome alignment of orangutan, mouse, dog, and opossum genomes to human. This alignment is produced by Multiz, a program that utilizes pairwise whole genome alignments of many species and, using a phylogenetic tree, im ...
... In this exercise, we will utilize the UCSC Genome Browser to view a refined whole genome alignment of orangutan, mouse, dog, and opossum genomes to human. This alignment is produced by Multiz, a program that utilizes pairwise whole genome alignments of many species and, using a phylogenetic tree, im ...
Relationship of Gene Expression and Chromosomal Abnormalities in Colorectal Cancer
... that was collected for our samples allowed us to select the best candidates for CGH or SNP arrays. We focused on a particular chromosomal region (specifically 20q) known to be frequently gained in colorectal cancer. We sampled both tumors that exhibited a clear coordinated increased expression, as w ...
... that was collected for our samples allowed us to select the best candidates for CGH or SNP arrays. We focused on a particular chromosomal region (specifically 20q) known to be frequently gained in colorectal cancer. We sampled both tumors that exhibited a clear coordinated increased expression, as w ...
Molecular analysis of the structure and expression of the RH... individuals with D--, Dc-, and DCw- gene complexes
... exons 4-6 and exons 9-10, respectively. The absence of the 23-kb fragment after hybridization with exon 7 or exon 8 suggested either a deletion of the relevant regions of the RHCE gene in the DC- gene complex or the presence of an unusual band comigrating with the 19 kb D-specific fragment. The exon ...
... exons 4-6 and exons 9-10, respectively. The absence of the 23-kb fragment after hybridization with exon 7 or exon 8 suggested either a deletion of the relevant regions of the RHCE gene in the DC- gene complex or the presence of an unusual band comigrating with the 19 kb D-specific fragment. The exon ...
Yeast genome evolution-the origin of the species
... important differences. Foremost among these is that, irrespective of the selection pressures operating on the new gene, it may originate by a variety of processes, including gene duplication, horizontal gene transfer (HGT) and de novo emergence of an open reading frame. Of these, gene duplication is ...
... important differences. Foremost among these is that, irrespective of the selection pressures operating on the new gene, it may originate by a variety of processes, including gene duplication, horizontal gene transfer (HGT) and de novo emergence of an open reading frame. Of these, gene duplication is ...
Metatranscriptome_Pipeline_Tutorial.doc
... generated by the sequencer to files that can be read by the visualization tool, here we illustrate the use of the pipeline by going through the various steps to illustrate the complexity of the process and the underlying tools and files used and generated by the pipeline. To illustrate the process w ...
... generated by the sequencer to files that can be read by the visualization tool, here we illustrate the use of the pipeline by going through the various steps to illustrate the complexity of the process and the underlying tools and files used and generated by the pipeline. To illustrate the process w ...
Title: Genes in the Postgenomic Era Authors: Paul E. Griffiths and
... classical genetics should not be thought of as merely a theory of heredity embodied in Mendel's laws and their later refinements (Waters 2004; Falk In Press). Instead, this theory of heredity functioned as an investigative tool with which geneticists could explore broader biological questions. The ...
... classical genetics should not be thought of as merely a theory of heredity embodied in Mendel's laws and their later refinements (Waters 2004; Falk In Press). Instead, this theory of heredity functioned as an investigative tool with which geneticists could explore broader biological questions. The ...
SURVEY AND SUMMARY Genomics of bacteria and archaea: the
... been completely sequenced (5). Moreover, inevitable biases (especially, toward medically important bacteria) in sequencing notwithstanding, these genomes are representative of the majority of recognized bacterial and archaeal phyla (Table 1). A common concern with regard to the representation of the ...
... been completely sequenced (5). Moreover, inevitable biases (especially, toward medically important bacteria) in sequencing notwithstanding, these genomes are representative of the majority of recognized bacterial and archaeal phyla (Table 1). A common concern with regard to the representation of the ...
The genomic sequence of Exiguobacterium chiriqhucha str
... Fabiano Thompson Additional Information and Declarations can be found on page 19 DOI 10.7717/peerj.3162 Copyright 2017 Gutiérrez-Preciado et al. Distributed under Creative Commons CC-BY 4.0 ...
... Fabiano Thompson Additional Information and Declarations can be found on page 19 DOI 10.7717/peerj.3162 Copyright 2017 Gutiérrez-Preciado et al. Distributed under Creative Commons CC-BY 4.0 ...
Cloning, characterization and in vitro and in planta expression of a
... enzymes that degrade cell wall polysaccharides, together with various sets of expression data, suggests that they are likely to influence plant–pathogen interactions. However, ...
... enzymes that degrade cell wall polysaccharides, together with various sets of expression data, suggests that they are likely to influence plant–pathogen interactions. However, ...
Gene prediction and Genome Annotation
... Isolate and clone cognate transcripts (as cDNA) sequence them and compare cDNA with genomic DNA It’s the ONLY secure method! BUT !!! • Cloning is time consuming. • Lowly expressed genes are difficult to detect. • The nucleotide sequence does not contain translation information. • Short read length o ...
... Isolate and clone cognate transcripts (as cDNA) sequence them and compare cDNA with genomic DNA It’s the ONLY secure method! BUT !!! • Cloning is time consuming. • Lowly expressed genes are difficult to detect. • The nucleotide sequence does not contain translation information. • Short read length o ...
Bacteria and Archaea: The Prokaryotic Domains
... Some microbial communities form layers in sediments, and others form clumps a meter or more in diameter. While some microbial communities are harmful to humans, others provide important services. They help us digest our food, break down municipal waste, and recycle organic matter in the environment. ...
... Some microbial communities form layers in sediments, and others form clumps a meter or more in diameter. While some microbial communities are harmful to humans, others provide important services. They help us digest our food, break down municipal waste, and recycle organic matter in the environment. ...
Multiple Mechanisms Contribute to Lateral Transfer of an
... isolated by following the Currier Nester protocol with minor modifications (Currier and Nester 1976; Pandeeti et al. 2011). The plasmid preparation made from Sphingobium fuliginis ATCC 27551 was directly used for tagging with minitransposon EZ-Tn5,R6Kgori/KAN-2. using the EZTn5 ,R6Kgori/KAN-2. insert ...
... isolated by following the Currier Nester protocol with minor modifications (Currier and Nester 1976; Pandeeti et al. 2011). The plasmid preparation made from Sphingobium fuliginis ATCC 27551 was directly used for tagging with minitransposon EZ-Tn5,R6Kgori/KAN-2. using the EZTn5 ,R6Kgori/KAN-2. insert ...
Archives of Microbiology
... fused and clustered in a phylogenetic tree next to the corresponding genes of Methanosarcina. The expression of vnfH in cells cultured in Mo-free medium and of nifH in Mo-grown cells was shown for the Wrst time by sequencing cDNA derived from cultures of A. azotica and Anabaena sp. CH1. The vnfH seq ...
... fused and clustered in a phylogenetic tree next to the corresponding genes of Methanosarcina. The expression of vnfH in cells cultured in Mo-free medium and of nifH in Mo-grown cells was shown for the Wrst time by sequencing cDNA derived from cultures of A. azotica and Anabaena sp. CH1. The vnfH seq ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.