Genome-sequence analysis of Acinetobacter johnsonii MB44
... Acinetobacter johnsonii is generally recognized as a nonpathogenic bacterium although it is often found in hospital environments. However, a newly identified isolate of this species from a frost-plant-tissue sample, namely, A. johnsonii MB44, showed significant nematicidal activity against the model ...
... Acinetobacter johnsonii is generally recognized as a nonpathogenic bacterium although it is often found in hospital environments. However, a newly identified isolate of this species from a frost-plant-tissue sample, namely, A. johnsonii MB44, showed significant nematicidal activity against the model ...
Comparative analysis of peanut NBS‐LRR gene clusters suggests
... Biotechnology Information (NCBI) nonredundant (nr) protein database. The coding sequences of the predicted genes in each region were used as query for BLASTN and TBLASTX searches against the corresponding homoeologous or orthologous regions with a cutoff of E < 10)05 to identify the conserved genes. ...
... Biotechnology Information (NCBI) nonredundant (nr) protein database. The coding sequences of the predicted genes in each region were used as query for BLASTN and TBLASTX searches against the corresponding homoeologous or orthologous regions with a cutoff of E < 10)05 to identify the conserved genes. ...
Bioinformatics: Bringing it all together
... Wisconsin package, the grandfather of molecular-biology packages (now sold by Accelrys), had to work with UNIX-based systems. Although these systems are still preferred by some, users can now point-and-click their way through a wide range of tasks on ordinary desktop computers. Another trend is the ...
... Wisconsin package, the grandfather of molecular-biology packages (now sold by Accelrys), had to work with UNIX-based systems. Although these systems are still preferred by some, users can now point-and-click their way through a wide range of tasks on ordinary desktop computers. Another trend is the ...
CONNECTIVE TISSUE LABORATORY Center for Medical Genetics
... sterile solution. It is essential to send the biopsy to our laboratory by a special courier within 48 hours after sampling. If a tissue culture facility is available in your institute, we prefer you to culture fibroblasts from the biopsy and to keep part of the culture frozen at your institute. We n ...
... sterile solution. It is essential to send the biopsy to our laboratory by a special courier within 48 hours after sampling. If a tissue culture facility is available in your institute, we prefer you to culture fibroblasts from the biopsy and to keep part of the culture frozen at your institute. We n ...
Module 8 – Diversity of Microbial World
... microorganisms can differ considerably with respect to ecological characteristics. The ability to cause disease in a particular host; and habitat preferences such as requirements for temperature, pH, oxygen, and osmotic concentration are examples of ecological characteristics. 4. Genetic analysis:- ...
... microorganisms can differ considerably with respect to ecological characteristics. The ability to cause disease in a particular host; and habitat preferences such as requirements for temperature, pH, oxygen, and osmotic concentration are examples of ecological characteristics. 4. Genetic analysis:- ...
Isolation of DNA from A Single Helminth Using New Developed Kit
... tion by agarose gel electrophoresis may be more reliable. The amount of the extracted DNA was low, when the worms were used for the DNA extraction promptly after removing from 70% ethanol solution. The important critical point for the DNA extraction is to ensure that the worm is well disrupted, homo ...
... tion by agarose gel electrophoresis may be more reliable. The amount of the extracted DNA was low, when the worms were used for the DNA extraction promptly after removing from 70% ethanol solution. The important critical point for the DNA extraction is to ensure that the worm is well disrupted, homo ...
The Value of MLPA in Waardenburg Syndrome - MRC
... Whole-gene sequencing enables discovery of point mutations and small alterations in the gene, but cannot reliably detect whole-exon or whole-gene copy number changes. Exon and/or whole-gene copy number changes have been reported for many genes resulting in a specific genetic disorder. Although there ...
... Whole-gene sequencing enables discovery of point mutations and small alterations in the gene, but cannot reliably detect whole-exon or whole-gene copy number changes. Exon and/or whole-gene copy number changes have been reported for many genes resulting in a specific genetic disorder. Although there ...
Isolation of DNA sequences on human chromosome 21
... Umadevi Tantravahi 1' 3, Gordon D. Stewart 6, Margaret Van Keuren 6, Gerard McNeil 1' 3, Sayon Roy 1' 3, David Patterson 5, Harry Drabkin 5, Marc Lalande 1'3, David M. Knrnit 6, and Samuel A. L a t t 1"2, 3, 4 1Genetics Division and Mental Retardation Center, The Children's Hospital, 2Howard Hughes ...
... Umadevi Tantravahi 1' 3, Gordon D. Stewart 6, Margaret Van Keuren 6, Gerard McNeil 1' 3, Sayon Roy 1' 3, David Patterson 5, Harry Drabkin 5, Marc Lalande 1'3, David M. Knrnit 6, and Samuel A. L a t t 1"2, 3, 4 1Genetics Division and Mental Retardation Center, The Children's Hospital, 2Howard Hughes ...
Computational approaches to predict bacteriophage–host
... and Botstein 1971; Susskind, Botstein and Wright 1974). The step where the genomes of phage and host interact can also be wrought with difficulty for a phage. If the host acquires a mutation in the genomic site where a lysogenic phage typically integrates, either integration or excision could be aff ...
... and Botstein 1971; Susskind, Botstein and Wright 1974). The step where the genomes of phage and host interact can also be wrought with difficulty for a phage. If the host acquires a mutation in the genomic site where a lysogenic phage typically integrates, either integration or excision could be aff ...
Bioinformatics Sequencing
... – Phylogenetic trees can be constructed based on comparison of the sequences of a molecule (example: 16S rRNA) taken from different species – Residues conserved during evolution play an important role ...
... – Phylogenetic trees can be constructed based on comparison of the sequences of a molecule (example: 16S rRNA) taken from different species – Residues conserved during evolution play an important role ...
Counting reads with summarizeOverlaps
... When the region of interest is defined by one or more ranges the features argument should be a GRangesList. In practice this could be a list of exons by gene or transcripts by gene or other similar relationships. The count result will be the same length as the GRangesList. For a list of exons by gen ...
... When the region of interest is defined by one or more ranges the features argument should be a GRangesList. In practice this could be a list of exons by gene or transcripts by gene or other similar relationships. The count result will be the same length as the GRangesList. For a list of exons by gen ...
Molecular Identification of Vibrio harveyi From Larval Stage of
... and, in many cases, at the level of genus and ...
... and, in many cases, at the level of genus and ...
VI.P.1 VI.P.2 Session VI. Environmental microbiology
... The Black Sea is unique marine ecosystem with high microbial biotechnology potential. The microbial communities are the integral part of the ecosystem and play the key role in all biological processes occurring in it. The data on the Black Sea bacterial diversity is mainly obtained by cultural metho ...
... The Black Sea is unique marine ecosystem with high microbial biotechnology potential. The microbial communities are the integral part of the ecosystem and play the key role in all biological processes occurring in it. The data on the Black Sea bacterial diversity is mainly obtained by cultural metho ...
ISOLATION AND CHARACTERIZATION OF FREE
... We are focusing on isolation of bacteriophage(s) specific to some free N2-fixer bacteria from rhizosphere and non-rhizosphere soils of Taif region of KSA as a first record for such study in Taif area (Makkah, Jeddah and Taif). A number of 10 bacterial isolates grown on the nitrogen-free specific med ...
... We are focusing on isolation of bacteriophage(s) specific to some free N2-fixer bacteria from rhizosphere and non-rhizosphere soils of Taif region of KSA as a first record for such study in Taif area (Makkah, Jeddah and Taif). A number of 10 bacterial isolates grown on the nitrogen-free specific med ...
Will Koning`s Summer Project
... I identified two bacterial genes showing similarity to a small region of human Interleukin-10 using a BLAST search (Altschul et al. 1990; Altschul et al. 1997). I did the BLAST search looking for ‘short nearly exact matches’ to the amino-acid sequence of human IL-10. These two bacterial genes were c ...
... I identified two bacterial genes showing similarity to a small region of human Interleukin-10 using a BLAST search (Altschul et al. 1990; Altschul et al. 1997). I did the BLAST search looking for ‘short nearly exact matches’ to the amino-acid sequence of human IL-10. These two bacterial genes were c ...
Chapter 1
... • After the first step is completed, DNA polymerase III “reads” the parental strand or template, catalyzing the polymerization of a complementary daughter strand • In the polymerization reaction – A pyrophosphate group is released as a phosphoester bond is formed between the 5'-phosphoryl group of t ...
... • After the first step is completed, DNA polymerase III “reads” the parental strand or template, catalyzing the polymerization of a complementary daughter strand • In the polymerization reaction – A pyrophosphate group is released as a phosphoester bond is formed between the 5'-phosphoryl group of t ...
Full Text - American Diabetes Association
... morphogenetic protein (BMP)-signaling pathway. The approach allows us to generate testable hypotheses from GWAS candidates falling in promoter regions and has the potential to help understand the functional impact of genetic variants in DN and other complex genetic diseases. DN is the leading cause ...
... morphogenetic protein (BMP)-signaling pathway. The approach allows us to generate testable hypotheses from GWAS candidates falling in promoter regions and has the potential to help understand the functional impact of genetic variants in DN and other complex genetic diseases. DN is the leading cause ...
seq.
... species B C D). The phylogeny suggests that seq’ and seq have similar function, and that this function was important in the evolution of the clade BCD. seq’ in B and seq’in C and D are orthologs and probably have the same function, whereas seq and seq’ in BCD probably have different function (the di ...
... species B C D). The phylogeny suggests that seq’ and seq have similar function, and that this function was important in the evolution of the clade BCD. seq’ in B and seq’in C and D are orthologs and probably have the same function, whereas seq and seq’ in BCD probably have different function (the di ...
Prioritizing curation of mouse genes by paucity of annotations and
... Our goal is to achieve comprehensive annotation of ciliary genes in the laboratory mouse based on experimental data by mid 2015. Based on our early results, we were able to annotate some genes that were not associated in the Mouse Genome Database (MGD) with literature tagged to be curated for Gene O ...
... Our goal is to achieve comprehensive annotation of ciliary genes in the laboratory mouse based on experimental data by mid 2015. Based on our early results, we were able to annotate some genes that were not associated in the Mouse Genome Database (MGD) with literature tagged to be curated for Gene O ...
ZFIN-Mar2006 - Gene Ontology Consortium
... ZFIN currently has 8 curators, and will add 2 more in the first half of 2006. Currently all of them split their time between project management / development activities and literature curation for all data types, including GO. As a result, only a fraction of each curator’s time is spent on GO curati ...
... ZFIN currently has 8 curators, and will add 2 more in the first half of 2006. Currently all of them split their time between project management / development activities and literature curation for all data types, including GO. As a result, only a fraction of each curator’s time is spent on GO curati ...
Articles - American Scientist
... viruses are ”life.” “I believe that the virus factory should be considered the actual virus organism when referring to a virus. Incidentally, in this interpretation, the living nature of viruses is undisputable, on the same footing as intracellular bacterial parasites.” The virion particle would the ...
... viruses are ”life.” “I believe that the virus factory should be considered the actual virus organism when referring to a virus. Incidentally, in this interpretation, the living nature of viruses is undisputable, on the same footing as intracellular bacterial parasites.” The virion particle would the ...
Array-based sequencing of filaggrin gene for
... with a conserved exon structure and likely shared functions in epithelial biology (Gan et al. 1990; Henry et al. 2012; Strasser et al. 2014). FLG transcription is initiated in exon 2 with the majority of the profilaggrin polyprotein produced from the extremely long (>12Kb) and highly repetitive exon ...
... with a conserved exon structure and likely shared functions in epithelial biology (Gan et al. 1990; Henry et al. 2012; Strasser et al. 2014). FLG transcription is initiated in exon 2 with the majority of the profilaggrin polyprotein produced from the extremely long (>12Kb) and highly repetitive exon ...
Phylogenetic Place of Guinea Pigs: No Support of the Rodent
... Graur et al’s ( 199 1) hypothesis that the guinea pig-like rodents have an evolutionary origin within mammals that is separate from that of other rodents (the rodent-polyphyly hypothesis) was reexamined by the maximum-likelihood method for protein phylogeny, as well as by the maximum-parsimony and n ...
... Graur et al’s ( 199 1) hypothesis that the guinea pig-like rodents have an evolutionary origin within mammals that is separate from that of other rodents (the rodent-polyphyly hypothesis) was reexamined by the maximum-likelihood method for protein phylogeny, as well as by the maximum-parsimony and n ...
Fungal - CBS-KNAW Fungal Biodiversity Centre
... No teleomorphs are known for the Cercospora species causing leaf spot on celery, sugar beet or maize, although there was an unconWrmed report of a teleomorph for C. zeae-maydis (Latterell and Rossi, 1977). Wang et al. (1998) were unable to Wnd evidence of the MAT-2 idiomorph in isolates of C. zeae-m ...
... No teleomorphs are known for the Cercospora species causing leaf spot on celery, sugar beet or maize, although there was an unconWrmed report of a teleomorph for C. zeae-maydis (Latterell and Rossi, 1977). Wang et al. (1998) were unable to Wnd evidence of the MAT-2 idiomorph in isolates of C. zeae-m ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.