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Nature Biotechnology, 21(4) - Weizmann Institute of Science
Nature Biotechnology, 21(4) - Weizmann Institute of Science

... Microarrays were constructed by spotting each of the aforemen(http://www.ncbi.nlm.nih.gov/LocusLink/RSfaq.html) mRNAs that tioned oligonucleotide probes onto treated glass slides in quadruplidid not give clear hybridization signals on our microarrays. Thus, in cate. The two counterpart oligonucleoti ...
GenBank Searches
GenBank Searches

... Resources. This will take you to a new window that will allow you to search for nucleotide sequences present in genbank. (If you want to search for other sequences (e.g. protein, SNPs etc.) you could do this by selecting a different database in the drop down menu in the box that reads “nucleotide” a ...
Bioinformatics - University of Colorado Denver
Bioinformatics - University of Colorado Denver

... COX-2. This achievement has paved the way for developing new therapies that bind more specifically to their target and therefore have fewer side effects. Understanding the enzyme structures of COX-1 and COX-2 helped researchers develop a drug that would only bind and inhibit COX-2. Many of the types ...
Mapping strategies for sequence reads (with focus on RNA-seq)
Mapping strategies for sequence reads (with focus on RNA-seq)

... uires a set of known junctions from the reference ond, the QPALMA pipeline’s initial mapping phase (Abouelhoda et al., 2004), a general-purpose suffix lignment program. Vmatch is a flexible, fast aligner, t is not designed to map short reads on machines ain memories, it is substantially slower than ...
CHEM 331 Problem Set #7
CHEM 331 Problem Set #7

... 11. Bacterial endospores form when the environment is no longer conducive to active cell metabolism. The soil bacterium Bacillus subtilis, for example, begins the process of sporulation when one or more nutrients are depleted. The end product is a small, metabolically dormant structure that can sur ...
Something`s Fishy
Something`s Fishy

... guanine, and cytosine. This sequence of A, T, G, and C is unique to each individual. Restriction enzymes cut DNA. Each restriction enzyme recognizes a specific group of “target” base pairs and makes a cut within this area. The resulting fragments are called restriction fragment length polymorphisms ...
GMM assessment: experiences from the evaluation of food enzymes
GMM assessment: experiences from the evaluation of food enzymes

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blast
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... The Expect value (E) is a parameter that describes the number of “hits” one can "expect" to see just by chance when searching a database of a particular size. It decreases exponentially as the Similarity Score (S) increases (inverse relationship). The higher the Similarity Score, the lower the E val ...
Immunome database for marsupials and monotremes Open Access
Immunome database for marsupials and monotremes Open Access

... first identified using either BLAST [10] or HMMER hidden Markov model [52] searches. Following this, best hits were either concatenated into genes or used to predict a full gene model using a gene prediction program. 516 wallaby genes were annotated based on opossum genes identified in Wong et al. 2 ...
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No Slide Title

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Nucleic Acid Biotechnology Techniques
Nucleic Acid Biotechnology Techniques

... • DNA undergoes reaction that incorporates radioactive isotope into the DNA • Autoradiography used to visualize image that has been exposed to radiolabeled oligonucleotides ...
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Item 6 - NHS England
Item 6 - NHS England

... infrastructure – consolidated genomic laboratory infrastructure for rare, and inherited diseases and cancer operating to a defined service model, and a transitional role for the existing Genomic Medicine Centres to establish comprehensive coverage and access to genomic medicine across the NHS. A com ...
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ppt

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Lab #1: Alu Lab, Part 1
Lab #1: Alu Lab, Part 1

... Introns are an example of some of this non-coding DNA. As you might imagine, the sequence of our introns is much more variable than the sequence of the coding portions of our genes (exons) as mutations in introns don’t necessarily impact the structure of the protein. We carry, and pass along, such m ...
ddPCR
ddPCR

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Particle Mesh Ewald(PME) method
Particle Mesh Ewald(PME) method

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14–3 Human Molecular Genetics
14–3 Human Molecular Genetics

... In 1990, scientists began the Human Genome Project. One goal was to identify and map the locations of all the genes in human DNA. Second goal was to determine the sequence of the 3 billion base pairs that make up human DNA. Slide 14 of 24 Copyright Pearson Prentice Hall ...
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... • Phylogenetic trees express the evolutionary relationships between a set of species • The relationship is inferred from the similarities and differences between homologue sequences • The inferred relationships are subject to influences from ...
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The genome organisation of vertebrates
The genome organisation of vertebrates

... Encyclopedia of the Human Genome—Author Stylesheet very low level in prokaryotes) of noncoding sequences. These can be both intergenic, between genes, and intragenic, within genes. The latter sequences, called introns, separate different coding stretches, or exons, of most eukaryotic genes. The int ...
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... 1. Collection of data 2. acquisition of random samples, graphical / tabular representation of data 3. problems related to combining probabilities, central tendencies and dispersion 4. problems related to chi-square 5. problems of goodness of fit and independent events 6. verification of genetic rati ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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