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Stabilizing synthetic data in the DNA of living organisms
Stabilizing synthetic data in the DNA of living organisms

... within a single genome, or even within a single cell, there is the possibility for problems to occur with the storage of data. In particular, the existence of two of the same DNA sequences within a bacterial genome is known to induce homologous DNA recombination (Kowalczykowski et al. 1994; Kuzminov ...
Drug resistance of bacteria commensal with Drosophila
Drug resistance of bacteria commensal with Drosophila

... fluoroquinolones, sulfonamides, trimethoprim, and nalidixic acid (rifampicin is not recommended because resistance emerges frequently by point mutation); 3) devise a procedure that minimizes the likelihood of selecting resistance. In our case, we first reduced the bacterial load and then simultaneou ...
NAME: ________________ DATE: ____________ BLOCK: _____
NAME: ________________ DATE: ____________ BLOCK: _____

... Find Homo sapiens (humans) in the table to the right and click on the “B” icon under the Tools header. If more than one build is displayed, select the one with the highest number, as this will be the most recent version. ...
GLYPHOSATE RESISTANCE Background / Problem
GLYPHOSATE RESISTANCE Background / Problem

... Modern sequencing methods (sequencing by synthesis, pyrosequencing) have catapulted sequencing into realm of population genetics Human genome took 10 years to sequence originally, and hundreds of millions of dollars Now we can do it in a week for <$2,000 ...
Homology-based cloning and expression analysis of Rf genes
Homology-based cloning and expression analysis of Rf genes

... The pentatricopeptide repeat (PPR) protein family was first defined and given this name in Arabidopsis thaliana by Small and Peeters (2000), and then it was realized that it is particularly prevalent in terrestrial plants as compared with other eukaryotes. With the accomplishment of whole-genome seq ...
DNA and Genetics in Biotechnology
DNA and Genetics in Biotechnology

... Although most DNA is packaged in chromosomes within the nucleus, mitochondria also have a small amount of their own DNA. This genetic material is known as mitochondrial DNA or mtDNA. In humans, mitochondrial DNA spans about 16,500 DNA building blocks (base pairs), representing a small fraction of th ...
How did HIV evolve?
How did HIV evolve?

... 1. Scroll down until yu see a series of boxes. 2. Click on the box labeled Session Tools. Now look through the menu of the long rectangular box and select Start New Session. Name the ...
Chapter 9
Chapter 9

... – Relative susceptibility of microorganisms – Germicides classified as high, intermediate, or low effectiveness – High—kill all pathogens, including endospores – Intermediate—kill fungal spores, protozoan cysts, viruses, pathogenic bacteria – Low—kill vegetative bacteria, fungi, protozoa, some virus ...
X chromosome in Xq28
X chromosome in Xq28

... The construction of physical and transcriptional maps of mammalian genomes, providing clues to a better understanding of genome organization, may also shed light on how chromosomal position can influence gene expression. In viruses and prokaryotes the position of genes is important and often essenti ...
Genomics presentation
Genomics presentation

... • Transcription factories constitute a source of genomic rearrangements and translocations. Many of them are responsible for the arise of cancer. • New genome-wide technologies are being applied to tease apart how DNA sequences interact, thereby helping to build up the human “translocatome”. ...
Conservation genetics of the endangered grassland earless dragon
Conservation genetics of the endangered grassland earless dragon

... More specifically to the ACT and Cooma populations, the differences we found between the two populations are substantial. The ACT and Cooma groups differ from one another by at least 5% (range based on ND4 data = 5.76% to 6.23%). Using a conservative mitochondrial calibration of 2% sequence divergen ...
Large-Scale High-Resolution Orthology Using Gene Trees
Large-Scale High-Resolution Orthology Using Gene Trees

... Finding Orthologous Groups René van der Heijden ...
A general method for gene isolation in tagging approaches
A general method for gene isolation in tagging approaches

... identify a particular insertion sequence as the causal agent of the observed mutant phenotype by conventional Southern-based methods. Traditionally, the number of insertion sequences per plant was reduced by time-consuming outcrossing to lines with low numbers of insertion elements. To circumvent th ...
Regulatory Protein and Their Binding Sites
Regulatory Protein and Their Binding Sites

... SQ5: What fraction of genes do you think are preceded by promoters? What fraction are preceded by CRP-binding sites? C. Regulation of cyanobacterial genes by environmental nitrogen The cyanobacterium Nostoc needs multiple layers of regulation to govern the expression of genes related to nitrogen uti ...
What Makes the “Blue” in Blueberries?
What Makes the “Blue” in Blueberries?

... • Identifying gene structure of Mybs in blueberry • Determine patterns in chromosomal arrangement of Myb genes • Compare each of these to that of closely related plants • Examine various Myb genes in the genome to determine significant differences that could affect function ...
Bacterial Transformation - Eastern Regional High School
Bacterial Transformation - Eastern Regional High School

... Changing the genes and phenotype of a bacteria by uptake of foreign/new DNA ...
DNA barcoding parasite organisms found in terrestrial
DNA barcoding parasite organisms found in terrestrial

... sequences with greater sequence quality, more nucleotides present per COI sequence, is needed, but, given the financial and time constraints on this project, finding sequence data and statistically relating these sequences to the published sequences of the NCBI database was a success. The Unknown or ...
avian dna sexing order form
avian dna sexing order form

... Customer does not provide this information in the required manner, DNA Solutions will assign the species according to the information indicated on the form. If the information provided creates ambiguity in regards to the species according to the criteria of DNA Solutions, the Customer will be requir ...
and interferon-inducible bovine Mx1 promoter
and interferon-inducible bovine Mx1 promoter

... a panel of ssRNA viruses. The bovine Mx system may also offer such an innate protection, provided it shares some molecular characteristics with the ‘antiviral’ systems known so far. ...
Biotechnology and Recombinant DNA
Biotechnology and Recombinant DNA

...  Uses probes to detect DNA sequences in restriction fragments separated using gel ...
article ()
article ()

... To further investigate this PLC nucleosomal diagnostic; we ask whether particular dinucleotides which are known to participate to the positioning and formation of. nucleosomes [26J (e.g. AA dinucleotides) would carry PLC specifically associated to eucaryotic genomes. This can be examined if one perf ...
Relating genes to function: identifying enriched transcription factors
Relating genes to function: identifying enriched transcription factors

... database of official, unified peak calls from 708 ENCODE ChIPSeq non-histone and non-control experiments, encompassing 220 transcription factor and treatment combinations across 91 cell types. We first represent each called peak by the genomic position of its apex to minimize the effect of broader p ...
BIOINFORMATICS MODULE I - Tetrahymena Genome Database
BIOINFORMATICS MODULE I - Tetrahymena Genome Database

... what an open reading frame is, and differences in codon usage between organisms. When ‘Transeq’ translates their coding sequence using the ciliate codon table, the first reading frame will be correct, naturally. Students will see a peptide beginning with methionine and ending with an asterisk (STOP) ...
Virtual Lab: DNA and Genes
Virtual Lab: DNA and Genes

... 2. What process have we talked about this year that allows us to obtain energy from our foods? ...
Population Genetics in the Post
Population Genetics in the Post

... •Haplotypes make a SNP map of the human genome redundant: as some SNPs will be transmitted together, we only need a subset of SNPs to tag the entire region. •NHGRI launched in October the HapMap project: a description of the set of haplotype blocks and the SNPs that tag them. The HapMap will be valu ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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