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Population Genetics in the Post
Population Genetics in the Post

... •Haplotypes make a SNP map of the human genome redundant: as some SNPs will be transmitted together, we only need a subset of SNPs to tag the entire region. •NHGRI launched in October the HapMap project: a description of the set of haplotype blocks and the SNPs that tag them. The HapMap will be valu ...
Unusual Pattern Detection in DNA Database Using KMP Algorithm
Unusual Pattern Detection in DNA Database Using KMP Algorithm

... single letter designations. This decreases the space necessary to store information and increases processing speed for analysis. While most biological databases contain nucleotide and protein sequence information, there are also databases, which include taxonomic information such as the structural a ...
Milestone2
Milestone2

... The GC content of a genome is the percentage of nucleotides in the genome that are either guanines or cytosines. Different genomes have widely varying GC contents. For example, the genomes of the bacteria Anaeromyxobacter have a GC content of about 75%, whereas the genomes of the bacteria Buchnera h ...
Advancements in Genetic Engineering
Advancements in Genetic Engineering

... pretty to form a family, the genetic components in their descendants are limited to sets of combinations. Every aspect of physiologic functions have at most three combinations, once the both parties of couple is determined, whether their kids are healthy or not at a certain aspect is almost unchange ...
Robust DNA Polymerase for PCR Application in Molecular Cloning
Robust DNA Polymerase for PCR Application in Molecular Cloning

... the proofreading activity of Pfu, the extension rate is 1.5-fold faster than Pfu, and the processivity (how long a chain of DNA it polymerizes before it falls off) of BR3 is also 1.5-fold longer than Pfu. The thermostability of BR3 polymerase has been tested by heat shock and SDS-PAGE, indicating th ...
Mitochondria tutorial
Mitochondria tutorial

... specific target DNA sequence. It has a wide variety of uses, from diagnostic (i.e., is a particular pathogen present in a given biopsy/hamburger/mayonnaise sample?) to forensic (i.e., whose sperm is on the blue dress? are Sally Hemming's descendants genetically related to Thomas Jefferson's descenda ...
Appendix D
Appendix D

... 25.5. Your sink drain and garbage disposal can harbor several species of viruses and bacteria. Some sinks can contain more bacteria than in a flushed toilet. So, every week you should sanitize your drain: pour a solution of 1 teaspoon of chlorine bleach in 1 quart of water down the drain. 25.6. In t ...
EAWAG news 56e: Genomic Islands and Horizontal Gene Transfer
EAWAG news 56e: Genomic Islands and Horizontal Gene Transfer

... happening to the chromosomal DNA. Or is it? Bacterial chromosomes are now known to harbor what is called “genomic islands”, regions which can cut themselves out of the chromosome, in some cases travel to other bacterial cells and reinsert into the recipient’s chromosome. Their function? Very often, ...
Adaptation to nocturnality - learning from avian genomes
Adaptation to nocturnality - learning from avian genomes

... convergent phenotypes. Nocturnality is an obvious trait, that, according to current phylogenies, is thought to have evolved multiple times in birds, and species from a number of distinct taxa are either fully or partially nocturnal [1, 2]. Modifications of the sensory system are among the most commo ...
PTC Lab Instructions/Information
PTC Lab Instructions/Information

... 2. Using what you know about genetics, SNPs, and the PTC gene, explain why it is possible for a person to be a “weak taster.” 3. Some studies have shown that PTC “tasters” are less likely to become smokers. Why do you think scientists are seeing this correlation? 4. How can the techniques described ...
2007GenomeInformaticsGMODPoster
2007GenomeInformaticsGMODPoster

... GMOD for Natural Diversity Data ...
Supplementary Information (doc 36K)
Supplementary Information (doc 36K)

... pellet in the tube was then dried for 1-2 hours in a clean bench and finally re-dissolved in 100 μl of Nanopure water (4°C, overnight). The extracted and purified DNA was stored at -20°C. PCR amplification of the V3 region of 16S rDNA gene was performed in two steps to obtain enough DNA for the anal ...
General enquiries on this form should be made to
General enquiries on this form should be made to

... The executive summary must not exceed 2 sides in total of A4 and should be understandable to the intelligent non-scientist. It should cover the main objectives, methods and findings of the research, together with any other significant events and options for new work. TILLING (Targetted Local Lesions ...
Sporulation genes in members of the low G+C Gram-type
Sporulation genes in members of the low G+C Gram-type

... Genomic analysis among Bacillus spp. Due to some indications that the experimental test did not give unequivocal results (E. Stackebrandt, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH-Braunschweig, Germany, personal communication) and the comparison of sequences of sporulation genes d ...
A Frameshift Mutation Leading to Type 1
A Frameshift Mutation Leading to Type 1

... Although type 1 deficiencies are much more common, the molecular basis underlying these defects remains largely uncharacterized. Previous studies have shown that both copies of the ATIII gene in the majority of these patients are grossly intact," which suggests that the molecular defects are likely ...
Chapter 5
Chapter 5

... copies of nonfunctional transposons. • There are many duplications of large chromosome regions. ...
Course Syllabus - Mahidol University International College
Course Syllabus - Mahidol University International College

... Lectures, classroom discussion, practice in environmental microbiology laboratory 12. Teaching Media Power point, transparencies, slides, audiovisual and hand-out 13. Measurement and evaluation of student achievement 13.1 The ability to better understand the basic knowledge and techniques in environ ...
Microbial Growth
Microbial Growth

... Stationary phase ...
From Free-Living to Endosymbiotic Life
From Free-Living to Endosymbiotic Life

... change in lifestyle produces in an organism. The ancestors of many bacterial obligate mutualists were probably free-living bacteria with the ability to infect insects and in some way benefit from that. At the beginning they were possibly parasites or commensalists but with time evolved to a mutualist ...
DNA Technology and Genomics  I.
DNA Technology and Genomics I.

... The bacterial clone will make the protein encoded by the foreign gene. The potential uses of cloned genes fall into two general categories. a. To produce a protein product. For example, bacteria carrying the gene for human growth hormone can produce large quantities of the hormone. b. To prepare man ...
Week 7-Microarrays
Week 7-Microarrays

... •  One bad quality sample does not affect the other array results, a problem in two-channel arrays •  Results are easily comparable to arrays from different ...
tree - Tecfa
tree - Tecfa

... Character-based (Sequence) methods 1. Constructs a phylogenetic tree based on the ...
BL414 Genetics Spring 2006  page Test 2
BL414 Genetics Spring 2006 page Test 2

... 1) (2.5pts) T or F: ___T_______ Bacterial genomes do not have many repetitive sequences, most of their genome is unique. 2) (2.5pts) T or F: ____F______ Genetic linkage in corn can be analyzed using asci tetrad analysis. 3) (2.5pts) T or F: _____F_____ The Holliday model is the currently accepted mo ...


... Department of Biology, Woods Hole Oceanographic Institution Increasing use of small model fish in drug discovery and mechanistic toxicology demands knowledge of cytochrome P450 (CYP) gene regulation and function. CYP enzymes catalyze oxidative transformation leading to activation or inactivation of ...
GEN - Mass-Defect Tagging for Proteomic Analysis
GEN - Mass-Defect Tagging for Proteomic Analysis

... 40,000 proteins, however, many are only subtly different from each other. Good differentiation techniques are thus essential to the generation of high-quality data. Like many measurements conducted in biology, MS measurements are often ratiometric rather than absolute. In MS, quantitative comparison ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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