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Species matter: the role of competition in the assembly of
Species matter: the role of competition in the assembly of

... which are based solely on gene sequence similarity, most often the 16S rRNA gene. OTUs based on the commonly used 97% or 99% identity cutoffs at the 16S rRNA locus are known to encompass large swaths of genomic (Staley, 2006; Goris et al., 2007) and ecological (Ward et al., 2006) diversity within th ...
Web resources
Web resources

... You may encounter a sequence and you will want to know what this protein does. The sequence databases are linked to the literature through ENTREZ, which allows simple searches of all databases for keywords. Medline is also linked through PUBMED which allows literature searches. The BLAST results are ...
Overlapping Co-clusters
Overlapping Co-clusters

... • Each row or column can be assigned to multiple row and column clusters respectively by certain probability based on their distances from respective cluster prototypes. This will produce overlapping coclustering. • Maximum overlapping co-clusters that could be obtained = k x l • Initialization of X ...
Document
Document

... • products are fed into thin capillary tube • 10 to 300 µm in diameter and ~ 50 cm long • applied electric field of up to 1,200 V/cm • higher fields can be used with smaller cross sections due to the ability to remove heat more rapidly • tag DNA with tag to “light up” strands across gel • radioactiv ...
Document - Fan Lab
Document - Fan Lab

... well-annotated genome and their ortholog sequences in another closely related genome, which hasn’t been annotated. The result a) can be used to compute the diverge time between two species through estimating average Ks and mutation rate; b) can be used to estimate how many ortholog sequence pairs ar ...
official course outline information
official course outline information

... By organizing the exercises as part of a single project, students get the sense of performing a complete cloning project, rather than just learning a collection of procedures. The complete course requires the students to perform DNA cloning techniques that are in common use in research molecular bio ...
Variation and Evolution of Genetic Networks
Variation and Evolution of Genetic Networks

... Arabidopsis lyrata ~95% seq. identity ~tiling Arabidopsis array: ~3M PM/MM features ...
Tài liệu PDF
Tài liệu PDF

... discovered. Small, virus-like particles called gene transfer agents (GTAs) transfer random genomic segments from one species of prokaryote to another. GTAs have been shown to be responsible for genetic changes, sometimes at a very high frequency compared to other evolutionary processes. The first GT ...
Polymerase Chain Reaction
Polymerase Chain Reaction

... Homozygous: An individual has two different alleles present at a specific locus Homozygous: An individual has two identical copies of the same allele present at a specific locus In situ hybridization: Use of a DNA or RNA probe to detect the presence of the complementary DNA sequence in cloned bacter ...
Data Mining in Ensembl with BioMart
Data Mining in Ensembl with BioMart

... • A data export tool • A quick table generator • A web interface to mine Ensembl data ...
Omics and Overview tutorial script
Omics and Overview tutorial script

... Can zoom in and out Semantic zooming means that information appears and disappears Highlighting and other display tools Can show / hide links between transported compounds and those same compounds inside the metabolic diagram Can highlight reactions and pathways Show highlight all by class Show clea ...
Bioinformatics/Computational Biology
Bioinformatics/Computational Biology

... field of industrial biotechnology, operating its own biotechnology capability out of the R&D lab in Wilton. INVISTA’s bio-scientists and engineers are working, alongside collaborators in academia and external technology companies, to develop new biotechnology processes and bring bio-derived products ...
DIS (1999) 82, 94-95 - Institut de Génétique Humaine
DIS (1999) 82, 94-95 - Institut de Génétique Humaine

... Key words: P-mediated transposition; enhancer trap; yellow gene. The P[lyB] enhancer trap vector (figure 1A) was designed to be used in P-element-mediated mutagenesis and enhancer detection in Drosophila melanogaster. It contains the ß-galactosidase reporter gene and the yellow gene as a transformat ...
Bioinformatics Exercises Over the last two decades, information has
Bioinformatics Exercises Over the last two decades, information has

... multiple sequence alignment d. Once you have defined these terms, find resources on the Internet which enable you to study them. 2. TIGR (The Institute for Genomic Research) Exercise. Open the TIGR site (http://www.tigr.org). Find the Comprehensive Microbial Resource. a. What 2001 publication descri ...
Supplementary Methods.
Supplementary Methods.

... study, we initially focused on human chromosome 16 and tested 79 elements (73 were human-fugu conserved, 4 were human-fugu-ultra conserved, and 2 were ultraconserved alone) from this chromosome but subsequently expanded the study to include 88 elements on other chromosomes (10 were human-fugu conser ...
Informed consent.
Informed consent.

... NGS studies generate a huge amount of data and it is mandatory to distinguish what is informative data and what isn´t. There are also some technical limitations that should be known to accurately interpret the results: • There are some regions in genes that are difficult to analyze. If this is the c ...
IJBT 10(2) 178-182
IJBT 10(2) 178-182

... performance. The experimental animals do not represent a random sample, nor do they reflect the overall genetic make-up of the population maintained at NDRI herd, since the males were selected as future bulls through a multi-stage selection procedure. More than 80% of the male calves born are auctio ...
De novo assembled expressed gene catalog of a
De novo assembled expressed gene catalog of a

... derived from six tissues of a Eucalyptus grandis x E. urophylla F1 hybrid clone and filtered to exclude low quality and ribosomal RNA-derived reads. Supplemental Table S2. The complete gene catalog from Arabidopsis thaliana (TAIR 9) was used to query the EucALL dataset and the Velvet assembled datas ...
Molecular diversity of thermophilic bacteria isolated from Pasinler
Molecular diversity of thermophilic bacteria isolated from Pasinler

... concentration ranges of 10.08%-29.42%. However, fatty acid 19:0 iso, which is present in all other remaining strains, is not found. These strains were identified as Bacillus spp. according to FAME data. The remaining 5 bacterial strains (G19B, P45, Ah23, P26, and M71) also showed similar FAME compos ...
Products of Modern Biotechnology
Products of Modern Biotechnology

... proteins is often significantly lower than bacteria ...
Sequencing Crop Genomes - Tropical Life Sciences Research
Sequencing Crop Genomes - Tropical Life Sciences Research

... as restriction fragment length polymorphism (RFLP), random amplification of polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), single sequence repeat (SSR), and single nucleotide polymorphisms (SNPs) have been identified and applied to improve breeding of several plants (Salgotra ...
Selective propagation of the clones
Selective propagation of the clones

... separate nucleic acids (DNA, RNA).  Northern blot: separation of RNA molecules on agarose gel, blotting and hybridizing with specific labeled probe or gene Southern blot: separation of DNA molecules on agarose or polyacrylamide ges, blotting to nylon or nitrocellulose membranes and hybridizing wit ...
Lab 9: Web Applications for Gene Family Evolution
Lab 9: Web Applications for Gene Family Evolution

... make up the ABC-transporter trans-membrane domains What is the “ABC tran”? Click on that link. This links to the Sanger Trust Institute database on protein families. This page is specifically for this domain. So, it's the transporter part of the protein and it is also involved in ATP-hydrolysis. Cli ...
Table 7. Summary statistics for the consensus gene set of Haliotis
Table 7. Summary statistics for the consensus gene set of Haliotis

... supply of abalone has increased fivefold since 1970’s. In order to prevent indiscreetly fishing abalones, legal landings from abalone fisheries have made fishery productions decreased gradually from 19,720 mt to 7,486 mt, but have made farm productions increase explosively from 50 mt to 103,464 mt i ...
How to measure DNA methylation
How to measure DNA methylation

... Functional genomic distribution ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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