Silene sex chromosome genetic map, p. 1 Expansion of
... and complete sex-linkage, based on population genetic evidence. Linkage groups were inferred using the JoinMap software version 4.0 (van Ooijen 2006) with a minimum LOD (logarithm of odds) score of 3. JoinMap searches for the best fitting order of markers by a trial and error procedure. The map for ...
... and complete sex-linkage, based on population genetic evidence. Linkage groups were inferred using the JoinMap software version 4.0 (van Ooijen 2006) with a minimum LOD (logarithm of odds) score of 3. JoinMap searches for the best fitting order of markers by a trial and error procedure. The map for ...
1st set of Journal Clubs this Wednesday!
... Prediction: If protein Gilbert and Müller-Hill isolated really was the lac repressor then it should bind to the lac operator in an inducer sensitive manner (i.e. the addition of inducer should prevent the repressor from binding to the operator). Experiment: Cohn and colleagues used a nitrocellulose ...
... Prediction: If protein Gilbert and Müller-Hill isolated really was the lac repressor then it should bind to the lac operator in an inducer sensitive manner (i.e. the addition of inducer should prevent the repressor from binding to the operator). Experiment: Cohn and colleagues used a nitrocellulose ...
Differential Gene Expression in the Siphonophore
... data to quantify expression. Some of these studies lack biological replication, which makes it difficult to assess the significance of the results. The wide variation in methods across these studies provide interesting glimpses into the benefits and drawbacks of different approaches for measuring ex ...
... data to quantify expression. Some of these studies lack biological replication, which makes it difficult to assess the significance of the results. The wide variation in methods across these studies provide interesting glimpses into the benefits and drawbacks of different approaches for measuring ex ...
Heuris`c)search:)FastA)and)BLAST)
... similarities between newly determined amino acid sequences and sequences already available in databases. Because of the algorithm's efficiency on many microcomputers, sensitive protein database searches may now become a routine procedure for molecular biologists. The method efficiently identifies re ...
... similarities between newly determined amino acid sequences and sequences already available in databases. Because of the algorithm's efficiency on many microcomputers, sensitive protein database searches may now become a routine procedure for molecular biologists. The method efficiently identifies re ...
The photosynthetic apparatus of Prochlorococcus
... is numerically the most abundant, and it can grow deeper in the euphotic zone (Partensky et al., 1999a) presumably because Chl b2 allows it to efficiently harvest the low light intensities and blue wavelengths characteristic of deep water (Moore et al., 1995; Morel et al., 1993). Prochlorococcus iso ...
... is numerically the most abundant, and it can grow deeper in the euphotic zone (Partensky et al., 1999a) presumably because Chl b2 allows it to efficiently harvest the low light intensities and blue wavelengths characteristic of deep water (Moore et al., 1995; Morel et al., 1993). Prochlorococcus iso ...
Analysis of expressed sequence tags from the Huperzia serrata leaf
... each were directly associated with ‘Cell’ and ‘Cell part’ functions, respectively. A total of 155 unique sequences (22.1%) were assigned to the ‘Macromolecular complex’ (Fig. 2A). In contrast, the majority of unique sequences in the molecular function category were assigned to ‘Catalytic activity’ ( ...
... each were directly associated with ‘Cell’ and ‘Cell part’ functions, respectively. A total of 155 unique sequences (22.1%) were assigned to the ‘Macromolecular complex’ (Fig. 2A). In contrast, the majority of unique sequences in the molecular function category were assigned to ‘Catalytic activity’ ( ...
Supporting Information Parfenov et al. 10.1073/pnas.1416074111
... million reads mapped) across 70% of the patient samples as not expressed (white with black outline in Fig. 2 and Figs. S4 and S5). For graphical representation of exon expression levels, we mean-centered the RPKM values and divided by their SD for each exon. The lengths of the composite exons were t ...
... million reads mapped) across 70% of the patient samples as not expressed (white with black outline in Fig. 2 and Figs. S4 and S5). For graphical representation of exon expression levels, we mean-centered the RPKM values and divided by their SD for each exon. The lengths of the composite exons were t ...
Morgan and Linkage
... two schematic sperm in panel D). As a result, the offspring usually inherits a combination of a parents paternal and maternal chromosomes. The probability that a recombination event occurs between two loci is a function of the distance between the two loci. The alleles at two loci that are far apart ...
... two schematic sperm in panel D). As a result, the offspring usually inherits a combination of a parents paternal and maternal chromosomes. The probability that a recombination event occurs between two loci is a function of the distance between the two loci. The alleles at two loci that are far apart ...
RNA Splicing
... • The mechanism excludes any splicing together of sequences representing different alleles • A typical mammalian gene has 7 to 8 exons spread out over ~16 kb . The exons are relatively short(~100 – 200 bp) and introns relatively long(>1 kb) • So, gene is interrupted while mRNA(~2.2 kb) is uninterru ...
... • The mechanism excludes any splicing together of sequences representing different alleles • A typical mammalian gene has 7 to 8 exons spread out over ~16 kb . The exons are relatively short(~100 – 200 bp) and introns relatively long(>1 kb) • So, gene is interrupted while mRNA(~2.2 kb) is uninterru ...
Predicting_tRNA_and_tmRNA_genes_12-2-16
... Predicting tRNA and tmRNA genes Revised December 1, 2016 ...
... Predicting tRNA and tmRNA genes Revised December 1, 2016 ...
Comparative sequence analysis of the long repeat regions and
... been resolved by revision of both the strain F and strain 17 sequences (Chou & Roizman, 1990; Chou et al., 1990; A. Dolan, E. McKie, A. R. MacLean & D. J. McGeoch, unpublished data), adding a further gene to the complement recognized for strain 17. I C P 3 4 . 5 appears to be an important determinan ...
... been resolved by revision of both the strain F and strain 17 sequences (Chou & Roizman, 1990; Chou et al., 1990; A. Dolan, E. McKie, A. R. MacLean & D. J. McGeoch, unpublished data), adding a further gene to the complement recognized for strain 17. I C P 3 4 . 5 appears to be an important determinan ...
Predicting tRNA and tmRNA genes Aragorn - SEA
... Predicting tRNA and tmRNA genes Revised December 1, 2016 ...
... Predicting tRNA and tmRNA genes Revised December 1, 2016 ...
Tutorial - QIAGEN Bioinformatics
... track is reversed. You can edit the parameters of various tools in the workflow by double-clicking on them and going through the corresponding wizards in the view area, or do it when running the workflow wizard. If you are using the first option, parameters can be set and locked to certain values, a ...
... track is reversed. You can edit the parameters of various tools in the workflow by double-clicking on them and going through the corresponding wizards in the view area, or do it when running the workflow wizard. If you are using the first option, parameters can be set and locked to certain values, a ...
What`s new - JSI medical systems
... If you use this setting the sequence is cut to the entered values for each ROI: e.g. setting is 5' 20 and 3' 30: All ROIs are cut to 20 bases before the exon and 30 bases after the exon in case the sequences defined on the enrichment chip are longer. The settings auto complete, check equal, cut prim ...
... If you use this setting the sequence is cut to the entered values for each ROI: e.g. setting is 5' 20 and 3' 30: All ROIs are cut to 20 bases before the exon and 30 bases after the exon in case the sequences defined on the enrichment chip are longer. The settings auto complete, check equal, cut prim ...
Conspiracy of silence among repeated transgenes
... variegation was indeed caused by heterochromatin formation was confirmed by showing suppression of silencing by suppressors of PEV, and more recent work has demonstrated additional heterochromatic properties of mini-white repeat arrays.(10) Heterochromatin formation at transgene arrays seems not to ...
... variegation was indeed caused by heterochromatin formation was confirmed by showing suppression of silencing by suppressors of PEV, and more recent work has demonstrated additional heterochromatic properties of mini-white repeat arrays.(10) Heterochromatin formation at transgene arrays seems not to ...
2q13 microduplications
... If a parent is found to have the same duplication as their child, the possibility of having another child with this duplication is 50 per cent in each pregnancy. If neither parent is found to have this chromosomal anomaly, it is unlikely that they will have another child with a 2q13 microduplication ...
... If a parent is found to have the same duplication as their child, the possibility of having another child with this duplication is 50 per cent in each pregnancy. If neither parent is found to have this chromosomal anomaly, it is unlikely that they will have another child with a 2q13 microduplication ...
Nixon Evidence
... number S42359) were used to examine 50,000 shotgun sequences from the genomic DNA of the WB isolate of G. lamblia (3, 54, 86). We estimate that these single-pass reads cover 98% of the coding capacity of the G. lamblia genome. Three unique G. lamblia ferredoxin genes, as well as two genes that encod ...
... number S42359) were used to examine 50,000 shotgun sequences from the genomic DNA of the WB isolate of G. lamblia (3, 54, 86). We estimate that these single-pass reads cover 98% of the coding capacity of the G. lamblia genome. Three unique G. lamblia ferredoxin genes, as well as two genes that encod ...
HapTree-X: An integrative Bayesian framework for haplotype
... the case of two heterozygous SNP sites, genotype calling tools cannot determine whether “mutant” alleles from different SNP loci are on the same chromosome or on different homologous chromosomes (i.e. compound heterozygote). In many cases, the latter can cause loss of function while the former is he ...
... the case of two heterozygous SNP sites, genotype calling tools cannot determine whether “mutant” alleles from different SNP loci are on the same chromosome or on different homologous chromosomes (i.e. compound heterozygote). In many cases, the latter can cause loss of function while the former is he ...
(S) tet Resistance Determinant Element Containing the Tetracycline
... pAM120 is used to probe the blots, HincII digestion shows similar hybridizing fragments, as would be expected from DNA containing an integrated copy of Tn916 but with the extra HincII fragment (see above). When the blots were probed with tet(M), there was no hybridization (data not shown). Taken tog ...
... pAM120 is used to probe the blots, HincII digestion shows similar hybridizing fragments, as would be expected from DNA containing an integrated copy of Tn916 but with the extra HincII fragment (see above). When the blots were probed with tet(M), there was no hybridization (data not shown). Taken tog ...
Recombination and clonal groupings within Helicobacter pylori from
... isolated from East Asians and Europeans (Atherton et al ., 1995; Ito et al ., 1998; Pan et al ., 1998; van Doorn et al ., 1998a), suggesting that recombination is rare between bacteria from different continents or that particular alleles are selected for in certain populations. However, a different ...
... isolated from East Asians and Europeans (Atherton et al ., 1995; Ito et al ., 1998; Pan et al ., 1998; van Doorn et al ., 1998a), suggesting that recombination is rare between bacteria from different continents or that particular alleles are selected for in certain populations. However, a different ...
Contamination controls when preparing archaeological remains for
... ancient DNA are the degradation of DNA over time and the contamination of ancient samples with modern DNA [11,15,22]. Physical and chemical degradation can destroy most of the DNA molecules contained in ancient remains. The minute amounts of preserved DNA are left in poor condition, damaged and brok ...
... ancient DNA are the degradation of DNA over time and the contamination of ancient samples with modern DNA [11,15,22]. Physical and chemical degradation can destroy most of the DNA molecules contained in ancient remains. The minute amounts of preserved DNA are left in poor condition, damaged and brok ...
RNA Polymerases
... regulation of their transcription. Some promoters such as the U6 small nuclear RNA (U6 snRNA ) and small RNA genes from the Epstein-Barr virus use only regulatory sequences upstream from their transcription start sites. The coding region of the U6 snRNA has a characteristic A box. However, this sequ ...
... regulation of their transcription. Some promoters such as the U6 small nuclear RNA (U6 snRNA ) and small RNA genes from the Epstein-Barr virus use only regulatory sequences upstream from their transcription start sites. The coding region of the U6 snRNA has a characteristic A box. However, this sequ ...
A Complex Suite of Forces Drives Gene Traffic from Drosophila X
... Because previously published analyses of gene duplication from the X chromosome to the autosomes in Drosophila have been limited to only retroposed genes and to only the D. melanogaster genome (Betrán et al. 2002; Dai et al. 2006; Bai et al. 2007), it is unclear whether these patterns of movement h ...
... Because previously published analyses of gene duplication from the X chromosome to the autosomes in Drosophila have been limited to only retroposed genes and to only the D. melanogaster genome (Betrán et al. 2002; Dai et al. 2006; Bai et al. 2007), it is unclear whether these patterns of movement h ...
Human genome
The human genome is the complete set of nucleic acid sequence for humans (Homo sapiens), encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria. Human genomes include both protein-coding DNA genes and noncoding DNA. Haploid human genomes, which are contained in germ cells (the egg and sperm gamete cells created in the meiosis phase of sexual reproduction before fertilization creates a zygote) consist of three billion DNA base pairs, while diploid genomes (found in somatic cells) have twice the DNA content. While there are significant differences among the genomes of human individuals (on the order of 0.1%), these are considerably smaller than the differences between humans and their closest living relatives, the chimpanzees (approximately 4%) and bonobos. Humans share 50% of their DNA with bananas.The Human Genome Project produced the first complete sequences of individual human genomes, with the first draft sequence and initial analysis being published on February 12, 2001. The human genome was the first of all vertebrates to be completely sequenced. As of 2012, thousands of human genomes have been completely sequenced, and many more have been mapped at lower levels of resolution. The resulting data are used worldwide in biomedical science, anthropology, forensics and other branches of science. There is a widely held expectation that genomic studies will lead to advances in the diagnosis and treatment of diseases, and to new insights in many fields of biology, including human evolution.Although the sequence of the human genome has been (almost) completely determined by DNA sequencing, it is not yet fully understood. Most (though probably not all) genes have been identified by a combination of high throughput experimental and bioinformatics approaches, yet much work still needs to be done to further elucidate the biological functions of their protein and RNA products. Recent results suggest that most of the vast quantities of noncoding DNA within the genome have associated biochemical activities, including regulation of gene expression, organization of chromosome architecture, and signals controlling epigenetic inheritance.There are an estimated 20,000-25,000 human protein-coding genes. The estimate of the number of human genes has been repeatedly revised down from initial predictions of 100,000 or more as genome sequence quality and gene finding methods have improved, and could continue to drop further. Protein-coding sequences account for only a very small fraction of the genome (approximately 1.5%), and the rest is associated with non-coding RNA molecules, regulatory DNA sequences, LINEs, SINEs, introns, and sequences for which as yet no function has been elucidated.