Engineering Acetyl Coenzyme A Supply: Functional Expression of a
... the mitochondrial matrix. However, in this yeast, acetyl-CoA generated in the mitochondrion cannot meet the requirement for cytosolic acetyl-CoA. Instead, a separate pathway known as the pyruvate dehydrogenase bypass, which involves pyruvate decarboxylase, acetaldehyde dehydrogenase, and acetyl-CoA ...
... the mitochondrial matrix. However, in this yeast, acetyl-CoA generated in the mitochondrion cannot meet the requirement for cytosolic acetyl-CoA. Instead, a separate pathway known as the pyruvate dehydrogenase bypass, which involves pyruvate decarboxylase, acetaldehyde dehydrogenase, and acetyl-CoA ...
Protein translation in Plasmodium parasites
... encoded by the nuclear genome, translated in the cytoplasm, and transported into the apicoplast using specific targeting signals [4]. Detection of plastid-encoded EF-Tu by western blot and immunofluorescence has provided evidence for translation in P. falciparum plastids [5]. Indirect evidence of ap ...
... encoded by the nuclear genome, translated in the cytoplasm, and transported into the apicoplast using specific targeting signals [4]. Detection of plastid-encoded EF-Tu by western blot and immunofluorescence has provided evidence for translation in P. falciparum plastids [5]. Indirect evidence of ap ...
Recycling of vitamin B12 and NAD+ within the Pdu
... Recent crystallography studies suggested that some Pdu MCP shell proteins, such as PduA, T and U, have pores that may mediate the transport of enzyme substrates/cofactors across the MCP shell. However, it’s possible that the cofactors might be regenerated in the local vicinity of the metabolic enzym ...
... Recent crystallography studies suggested that some Pdu MCP shell proteins, such as PduA, T and U, have pores that may mediate the transport of enzyme substrates/cofactors across the MCP shell. However, it’s possible that the cofactors might be regenerated in the local vicinity of the metabolic enzym ...
and Rhizobiales-Like PPP-Family Protein Phosphatases from
... biosynthesis and mitochondrial intermembrane space processes, respectively. Lastly, phenotypic analysis of atslp2 insertional mutant and 35S::AtSLP2 over-expression plants revealed biological involvement in regulating gibberellic acid-related processes during seed germination as well as ...
... biosynthesis and mitochondrial intermembrane space processes, respectively. Lastly, phenotypic analysis of atslp2 insertional mutant and 35S::AtSLP2 over-expression plants revealed biological involvement in regulating gibberellic acid-related processes during seed germination as well as ...
Progesterone-stimulated intracellular calcium increase in human
... medium, centrifuged and washed twice in a buffer (buffer 1) containing 20 mM Tris, 2 mM EDTA, 1 mM EGTA, 2 mM PMSF, 330 mM sucrose, 50 mM 2βmercaptoethanol, 5% glycerol, 5 µg/ml leupeptin, 0.5 mg/ml bacitracin and 50 µg/ml soybean trypsin inhibitor. All subsequent passages were performed on ice. Spe ...
... medium, centrifuged and washed twice in a buffer (buffer 1) containing 20 mM Tris, 2 mM EDTA, 1 mM EGTA, 2 mM PMSF, 330 mM sucrose, 50 mM 2βmercaptoethanol, 5% glycerol, 5 µg/ml leupeptin, 0.5 mg/ml bacitracin and 50 µg/ml soybean trypsin inhibitor. All subsequent passages were performed on ice. Spe ...
thèse - Université Evry Val d`Essonne
... hypothesis of mitochondrial origin, popularized in the early seventies by Lynn Margulis’s book “the origin of eukaryotic cells”, stating that mitochondria evolved from a bacterial progenitor through symbiosis with an amitochondrial eukaryotic cell. These primitive eukaryotes were formally put in the ...
... hypothesis of mitochondrial origin, popularized in the early seventies by Lynn Margulis’s book “the origin of eukaryotic cells”, stating that mitochondria evolved from a bacterial progenitor through symbiosis with an amitochondrial eukaryotic cell. These primitive eukaryotes were formally put in the ...
A conserved N-‐terminal domain of Rif2 regulates telomere length in
... The regulation of telomere length equilibrium is essential for cell growth and survival since critically short telomeres signal DNA damage and cell cycle arrest. While the broad principles of length regulatio ...
... The regulation of telomere length equilibrium is essential for cell growth and survival since critically short telomeres signal DNA damage and cell cycle arrest. While the broad principles of length regulatio ...
BIOGENESIS OF MITOCHONDRIAL ATPase Studies on
... have been recovered in the postribosomal supernatant after cell breakage [11]. These results strongly suggest that OSCP, like F 1 is provided by the extramitochondrial system. Remarkably, OSCP from yeast has not been found to form a compJex with F l ' It is bound, however, to OSCP-depleted membranes ...
... have been recovered in the postribosomal supernatant after cell breakage [11]. These results strongly suggest that OSCP, like F 1 is provided by the extramitochondrial system. Remarkably, OSCP from yeast has not been found to form a compJex with F l ' It is bound, however, to OSCP-depleted membranes ...
AraC Protein, Regulation of the L-arabinose Operon in Escherichia
... (Greenblatt & Schleif, 1971). This assay allowed the purification of modest amounts of AraC and the determination of many of the protein’s DNA-binding properties. Clearly, a substantial overproduction of the AraC protein is necessary for full purification. Indeed, as the molecular biology tools for ...
... (Greenblatt & Schleif, 1971). This assay allowed the purification of modest amounts of AraC and the determination of many of the protein’s DNA-binding properties. Clearly, a substantial overproduction of the AraC protein is necessary for full purification. Indeed, as the molecular biology tools for ...
Shigella boydii Involved in O-antigen Synthesis By
... Figure 1. A model of inner and outer cell membranes of E.coli K-12. ......................... - 2 Figure 2. An example of a cell surface LPS, from E.coli O111:B4. ............................. - 3 Figure 3. Three O-antigen repeating unit structures that are identical in different serotypes of Shigel ...
... Figure 1. A model of inner and outer cell membranes of E.coli K-12. ......................... - 2 Figure 2. An example of a cell surface LPS, from E.coli O111:B4. ............................. - 3 Figure 3. Three O-antigen repeating unit structures that are identical in different serotypes of Shigel ...
Structure-Based Prediction of Asparagine and Aspartate
... Monoclonal antibodies (mAbs) and proteins containing antibody domains are the most prevalent class of biotherapeutics in diverse indication areas. Today, established techniques such as immunization or phage display allow for an efficient generation of new mAbs. Besides functional properties, the sta ...
... Monoclonal antibodies (mAbs) and proteins containing antibody domains are the most prevalent class of biotherapeutics in diverse indication areas. Today, established techniques such as immunization or phage display allow for an efficient generation of new mAbs. Besides functional properties, the sta ...
Early events in protein folding
... usually taken as a signature of barrier-less folding51–53. It should, however, be noted that downhill folding reactions may also show exponential kinetics, depending on the roughness of the free energy surface54,55. Some mutant variants of the λ-repressor fragment, λ6-85, fold on a timescale close t ...
... usually taken as a signature of barrier-less folding51–53. It should, however, be noted that downhill folding reactions may also show exponential kinetics, depending on the roughness of the free energy surface54,55. Some mutant variants of the λ-repressor fragment, λ6-85, fold on a timescale close t ...
Soybean Meal – An Exceptional Protein Source
... Soybean meal has long been considered an outstanding source of supplemental protein in diets for livestock and poultry. In fact, soybean meal is sometimes referred to as the "gold standard" because other protein sources are often compared to it. Soybean meal is rich in highly digestible protein, and ...
... Soybean meal has long been considered an outstanding source of supplemental protein in diets for livestock and poultry. In fact, soybean meal is sometimes referred to as the "gold standard" because other protein sources are often compared to it. Soybean meal is rich in highly digestible protein, and ...
Purification of Arrestin from Bovine Retinas
... It has been reported that bovine arrestin may contain lipids and carbohydrate moieties, covalently attached to the protein (for review, see Ref. 22). It was also reported that arrestin can be modified substoichiometrically by phosphorylation, presumably by protein kinase C (23). Mass spectroscopic a ...
... It has been reported that bovine arrestin may contain lipids and carbohydrate moieties, covalently attached to the protein (for review, see Ref. 22). It was also reported that arrestin can be modified substoichiometrically by phosphorylation, presumably by protein kinase C (23). Mass spectroscopic a ...
MD1-103 Protein Stability Combo Kit
... • Optimize purification and storage conditions. • Screen for global parameters e.g. pH, salt concentrations, buffer type and concentration. • Tested on more than 200 different protein constructs. • Suits a wide range of proteins (small, large, complex, DNA binding proteins etc.) and applications. • ...
... • Optimize purification and storage conditions. • Screen for global parameters e.g. pH, salt concentrations, buffer type and concentration. • Tested on more than 200 different protein constructs. • Suits a wide range of proteins (small, large, complex, DNA binding proteins etc.) and applications. • ...
2nd_lecture
... Sequence Determination Frederick Sanger was the first - in 1953, he sequenced the two chains of insulin. • Sanger's results established that all of the molecules of a given protein have the same sequence • Proteins can be sequenced in two ways: - real amino acid sequencing - sequencing the correspon ...
... Sequence Determination Frederick Sanger was the first - in 1953, he sequenced the two chains of insulin. • Sanger's results established that all of the molecules of a given protein have the same sequence • Proteins can be sequenced in two ways: - real amino acid sequencing - sequencing the correspon ...
4. characterisation of novel proteins
... Refined soybean oil from MON87769 contains 20-30% SDA (% total fatty acids), and 5-8% gamma-linolenic acid (GLA), neither of which is present in conventional soybean oil. SDA soybean oil also contains slightly higher levels of alpha-linolenic acid (ALA) and palmitic acid as well as lower levels of o ...
... Refined soybean oil from MON87769 contains 20-30% SDA (% total fatty acids), and 5-8% gamma-linolenic acid (GLA), neither of which is present in conventional soybean oil. SDA soybean oil also contains slightly higher levels of alpha-linolenic acid (ALA) and palmitic acid as well as lower levels of o ...
Minimal metabolic pathway structure is consistent with
... networks of Escherichia coli (Orth et al, 2011) and Saccharomyces cerevisiae (Mo et al, 2009). They contain 750 and 332 pathways, respectively, representing the dimensions of the two null spaces (see Supplementary Dataset S1). For each calculated MinSpan pathway, we grouped the “gene-protein-reactio ...
... networks of Escherichia coli (Orth et al, 2011) and Saccharomyces cerevisiae (Mo et al, 2009). They contain 750 and 332 pathways, respectively, representing the dimensions of the two null spaces (see Supplementary Dataset S1). For each calculated MinSpan pathway, we grouped the “gene-protein-reactio ...
catalogue 2012 - olimp
... principle which involves a special queuing mode following the said principle -- this means that the batch manufactured and stored as the first one is used for manufacturing process in the first place. Both the samples of raw materials and semi-finished products as well as end products undergo detail ...
... principle which involves a special queuing mode following the said principle -- this means that the batch manufactured and stored as the first one is used for manufacturing process in the first place. Both the samples of raw materials and semi-finished products as well as end products undergo detail ...
PROTEIN PHOSPHORYLATION AND CELLULAR REGULATION, I by
... ed in skeletal muscle in two different forms that they designated a s phosphorylase b and phosphorylase a (2,3). The a form was purified and obtained as a crystalline enzyme. Kinetically, it was shown that phosphorylas e b required high concentrations of 5’-AMP for activity whereas phosphorylase a w ...
... ed in skeletal muscle in two different forms that they designated a s phosphorylase b and phosphorylase a (2,3). The a form was purified and obtained as a crystalline enzyme. Kinetically, it was shown that phosphorylas e b required high concentrations of 5’-AMP for activity whereas phosphorylase a w ...
The Role of the Bundle Sheath in the Leaf Development of C3 plants
... purine metabolism was assessed with systematic growth screens. Re was first described more than fifty years ago (Rédei and Hironyo, 1964), morphologically described and genetically mapped about forty years later (González-Bayón et al., 2006). Transcriptional profiling, extensive metabolite analysis, ...
... purine metabolism was assessed with systematic growth screens. Re was first described more than fifty years ago (Rédei and Hironyo, 1964), morphologically described and genetically mapped about forty years later (González-Bayón et al., 2006). Transcriptional profiling, extensive metabolite analysis, ...
Interactome
In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.