Functional characterization of rare FOXP2 variants in
... dimerization, and interaction with other proteins. To shed further light on molecular functions of FOXP2, we characterized the interaction between this transcription factor and co-repressor proteins of the C-terminal binding protein (CTBP) family. Finally, we analysed the functional significance of ...
... dimerization, and interaction with other proteins. To shed further light on molecular functions of FOXP2, we characterized the interaction between this transcription factor and co-repressor proteins of the C-terminal binding protein (CTBP) family. Finally, we analysed the functional significance of ...
Supplemental Table 3
... [evidence IEA]; Catalysis of the hydrolysis of peptide bonds by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions [goid 8237] [evidence IEA]; Interacting selectively with zinc (Z ...
... [evidence IEA]; Catalysis of the hydrolysis of peptide bonds by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions [goid 8237] [evidence IEA]; Interacting selectively with zinc (Z ...
Conformational changes in actin–myosin isoforms probed by Ni(II
... confirming the data obtained with the nucleotide-free myosin motor domain, and its relevance to actin binding, we have taken a solution-based approach to examine actin–myosin complexes through the use of Ni(II)Gly–Gly–His metallopeptides. Peptides of the general form Xaa–Xaa–His bind avidly to Cu(II ...
... confirming the data obtained with the nucleotide-free myosin motor domain, and its relevance to actin binding, we have taken a solution-based approach to examine actin–myosin complexes through the use of Ni(II)Gly–Gly–His metallopeptides. Peptides of the general form Xaa–Xaa–His bind avidly to Cu(II ...
Crosslinking Technical Handbook
... contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. The availability of several chemical groups in proteins and peptides make them targets for conjugation and for study using crosslinking methods. Crosslinkers also are commonly used ...
... contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. The availability of several chemical groups in proteins and peptides make them targets for conjugation and for study using crosslinking methods. Crosslinkers also are commonly used ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
... biosynthesis, and leucine biosynthesis pathways, are primary targets of the tRNA trafficking processes. I confirmed the microarray data by both Northern and Western blot analyses. Levels of all tested target proteins involved in such amino acid biogenesis pathways are down-regulated when the tRNA n ...
... biosynthesis, and leucine biosynthesis pathways, are primary targets of the tRNA trafficking processes. I confirmed the microarray data by both Northern and Western blot analyses. Levels of all tested target proteins involved in such amino acid biogenesis pathways are down-regulated when the tRNA n ...
unexpected consequences for sense codon reassignment
... Identification of inosine in tRNA anticodons The complement of tRNA molecules was extracted from NEB 5-alpha cells harboring a pWB Ultra plasmid (38). Briefly, RNA was precipitated by phenol:chloroform extraction from cell lysates; a detailed protocol is provided in the Supplementary Material (S5). ...
... Identification of inosine in tRNA anticodons The complement of tRNA molecules was extracted from NEB 5-alpha cells harboring a pWB Ultra plasmid (38). Briefly, RNA was precipitated by phenol:chloroform extraction from cell lysates; a detailed protocol is provided in the Supplementary Material (S5). ...
A motif and amino acid bias bioinformatics
... The wide range of potential functions of these molecules lies in the diversity of protein backbones and ...
... The wide range of potential functions of these molecules lies in the diversity of protein backbones and ...
Unveiling the physiological and molecular basis of Mycobacterium
... proportion of infecting bacilli is able to survive and strive among the challenging conditions present in the host’s macrophage. Current models of mycobacterial persistence invoke the presence of dormant populations of non-growing cells that reactivate during host immunosuppression. Bacterial biofil ...
... proportion of infecting bacilli is able to survive and strive among the challenging conditions present in the host’s macrophage. Current models of mycobacterial persistence invoke the presence of dormant populations of non-growing cells that reactivate during host immunosuppression. Bacterial biofil ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
... interaction in L12 dimer, had no functional relevance and was contradicted by an NMR structure where both the hinges were seen in fully extended form (9,11). Thus it is now universally accepted that the L12 dimer is ‘antiparallel’ where NTDs of two L12 molecules form a four-helix bundle dimer occupy ...
... interaction in L12 dimer, had no functional relevance and was contradicted by an NMR structure where both the hinges were seen in fully extended form (9,11). Thus it is now universally accepted that the L12 dimer is ‘antiparallel’ where NTDs of two L12 molecules form a four-helix bundle dimer occupy ...
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES
... cannot freely move across membrane barriers, it must be formed in the subcellular compartments in which it will be utilized, i.e. the plastid and the cytosol. Hence, ACCases occur in each of these compartments to generate malonylCOA. In most flowering plants, including Arabidopsis, there are two str ...
... cannot freely move across membrane barriers, it must be formed in the subcellular compartments in which it will be utilized, i.e. the plastid and the cytosol. Hence, ACCases occur in each of these compartments to generate malonylCOA. In most flowering plants, including Arabidopsis, there are two str ...
Attachment 1 - Food Standards Australia New Zealand
... potential costs and benefits of the proposed change to the Code from stakeholders is highly desirable. Claims made in submissions should be supported wherever possible by referencing or including relevant studies, research findings, trials, surveys etc. Technical information should be in sufficient ...
... potential costs and benefits of the proposed change to the Code from stakeholders is highly desirable. Claims made in submissions should be supported wherever possible by referencing or including relevant studies, research findings, trials, surveys etc. Technical information should be in sufficient ...
The C-terminal domain of the Rhizobium leguminosarum
... in the presence of hesperetin. Derivatives of pIJ1089 and pIJ1887 containing TnphoA were transferred to R. leguminosarum strain 8401 by conjugation. NodC–PhoA fusions expressing high levels of alkaline phosphatase could be identified in R. leguminosarum on plates containing hesperetin to induce nod ...
... in the presence of hesperetin. Derivatives of pIJ1089 and pIJ1887 containing TnphoA were transferred to R. leguminosarum strain 8401 by conjugation. NodC–PhoA fusions expressing high levels of alkaline phosphatase could be identified in R. leguminosarum on plates containing hesperetin to induce nod ...
The Vitamin K-dependent Carboxylase*
... protein is then secreted and circulates in the blood as a zymogen. The importance of the removal of the pro sequence for the normal function of a VKD blood protein is exemplified by the identification of Hemophilia B patients that have a point mutation at one of these conserved residues (e. g. R –4 ...
... protein is then secreted and circulates in the blood as a zymogen. The importance of the removal of the pro sequence for the normal function of a VKD blood protein is exemplified by the identification of Hemophilia B patients that have a point mutation at one of these conserved residues (e. g. R –4 ...
Gene Section FST (follistatin) Atlas of Genetics and Cytogenetics
... bound activin is unable to initiate signal transduction and consequently follistatin is a potent antagonist of physiological activin signals. Of the three follistatin domains present in all follistatin isoforms, (Shimasaki et al., 1988) the first two, but not the third, are necessary for activin A b ...
... bound activin is unable to initiate signal transduction and consequently follistatin is a potent antagonist of physiological activin signals. Of the three follistatin domains present in all follistatin isoforms, (Shimasaki et al., 1988) the first two, but not the third, are necessary for activin A b ...
Studying the Interaction Profiles of Nonnatural Amino Acids –
... their respective tasks in nature, proteins have evolved by means of selective pressure.1 The particular functionality of peptides and proteins is created by the physical and chemical properties of their building blocks, i.e. amino acids, which are precisely arranged in a threedimensional structure t ...
... their respective tasks in nature, proteins have evolved by means of selective pressure.1 The particular functionality of peptides and proteins is created by the physical and chemical properties of their building blocks, i.e. amino acids, which are precisely arranged in a threedimensional structure t ...
Developement of strategies for the isotopic labeling of methyl
... enhances the intensity of detectable long-range nOes by a factor 4. The described protocol also permits the suppression of spurious correlations, especially harmful for structural studies based on detection/analysis of nOes. To make an efficient use of the obtained high quality NMR spectra using thi ...
... enhances the intensity of detectable long-range nOes by a factor 4. The described protocol also permits the suppression of spurious correlations, especially harmful for structural studies based on detection/analysis of nOes. To make an efficient use of the obtained high quality NMR spectra using thi ...
Chlamydia Exploit the Mammalian Tryptophan-Depletion
... distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academ ...
... distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academ ...
METABOLIC CUES AND REGULATORY PROTEINS
... macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates between at least two distinct phenotypic phases: a non-infectious, replicative form required for intracellular growth and an infectious, transmissive form that enhances ...
... macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates between at least two distinct phenotypic phases: a non-infectious, replicative form required for intracellular growth and an infectious, transmissive form that enhances ...
Queuosine formation in eukaryotic tRNA occurs via a mitochondria
... Eubacteria are unique in their ability to synthesize Q. As part of this biosynthetic process, the eubacterial TGT enzyme inserts the Q-precursor molecule, 7-aminomethyl-7-deazaguanine (preQ1) into tRNA, which is then converted to Q by two further enzymatic steps at the tRNA level (6). Eukaryotes by ...
... Eubacteria are unique in their ability to synthesize Q. As part of this biosynthetic process, the eubacterial TGT enzyme inserts the Q-precursor molecule, 7-aminomethyl-7-deazaguanine (preQ1) into tRNA, which is then converted to Q by two further enzymatic steps at the tRNA level (6). Eukaryotes by ...
The effect of higher protein human milk fortifier on growth in preterm
... growth – were assessed weekly. Lean mass gain, which is better associated with adult metabolic health outcomes than adipose tissue gain in the in-hospital period, was also assessed weekly. Blood and urine chemistry markers were assessed weekly and every two weeks, respectively, as an assessment of p ...
... growth – were assessed weekly. Lean mass gain, which is better associated with adult metabolic health outcomes than adipose tissue gain in the in-hospital period, was also assessed weekly. Blood and urine chemistry markers were assessed weekly and every two weeks, respectively, as an assessment of p ...
Life in the slow lane: molecular mechanisms of estivation
... Organisms that incorporate facultative hypometabolism into their lifestyle (e.g. estivators, hibernators, anoxia-tolerant species) must implement a suite of control mechanisms that both coordinate an overall steep suppression of metabolic rate and adjust the relative rates of many cell functions. Th ...
... Organisms that incorporate facultative hypometabolism into their lifestyle (e.g. estivators, hibernators, anoxia-tolerant species) must implement a suite of control mechanisms that both coordinate an overall steep suppression of metabolic rate and adjust the relative rates of many cell functions. Th ...
The Major Component of the Paraflagellar Rod of Trypanosoma
... similarity to the intermediate filament proteins (solubility properties, amino acid composition, and high degree of helicity), the PFR protein does not belong in this class of cytoskeletal proteins. The PFR protein is coded for by two tandemly linked genes of identical nucleotide sequence. Both gene ...
... similarity to the intermediate filament proteins (solubility properties, amino acid composition, and high degree of helicity), the PFR protein does not belong in this class of cytoskeletal proteins. The PFR protein is coded for by two tandemly linked genes of identical nucleotide sequence. Both gene ...
CO2 and O2 Distribution in Rubisco Suggests the
... This indicates that O2 is hardly soluble in any environment, whereas CO2 is. Residues That Have a High Affinity for CO2. Virtually all residues are in contact with a gas molecule within 20 ns, but the interior of the subunit is less accessible than the surface. This needs to be considered when attempt ...
... This indicates that O2 is hardly soluble in any environment, whereas CO2 is. Residues That Have a High Affinity for CO2. Virtually all residues are in contact with a gas molecule within 20 ns, but the interior of the subunit is less accessible than the surface. This needs to be considered when attempt ...
Interactome
In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.