Chapter 1 Non-Canonical Amino Acids in Protein Engineering
... auxotrophic for both isoleucine and valine, were evaluated as expression hosts for the generation of proteins containing Tfv. The wild-type aaRS activity in these cells was insufficient for production of proteins containing Tfv, but incorporation of one of the diastereomers of Tfv (2S,3R-Tfv) into t ...
... auxotrophic for both isoleucine and valine, were evaluated as expression hosts for the generation of proteins containing Tfv. The wild-type aaRS activity in these cells was insufficient for production of proteins containing Tfv, but incorporation of one of the diastereomers of Tfv (2S,3R-Tfv) into t ...
An Engineered Aryl Azide Ligase for Site-Specific
... PPI detection by photo-cross-linking has considerable advantages over other more commonly used PPI detection methods, such as the yeast two-hybrid technique,[3] protein complementation assays (based on green fluorescent protein,[4] split b-lactamase,[5] and split luciferase),[6, 7] and fluorescence ...
... PPI detection by photo-cross-linking has considerable advantages over other more commonly used PPI detection methods, such as the yeast two-hybrid technique,[3] protein complementation assays (based on green fluorescent protein,[4] split b-lactamase,[5] and split luciferase),[6, 7] and fluorescence ...
Infrared spectroscopic studies: from small molecules to large.
... Infrared (IR) spectroscopy has been around since the discovery of IR light by Friedrich Wilhelm Herschel in 1800. However, until 1940’s, IR studies involved only water and small organic molecules, because of the long measurement times and inaccessibility of the instruments. In 1940s came the first c ...
... Infrared (IR) spectroscopy has been around since the discovery of IR light by Friedrich Wilhelm Herschel in 1800. However, until 1940’s, IR studies involved only water and small organic molecules, because of the long measurement times and inaccessibility of the instruments. In 1940s came the first c ...
Homology modeling workshop
... • Restraints were obtained also from a statistical analysis of the relationships from a large database of pairs of homologous structures. • Various correlations were obtained, e.g. correlations between CaCa distances. These relationships can be used directly as spatial restraints. • Restraints and C ...
... • Restraints were obtained also from a statistical analysis of the relationships from a large database of pairs of homologous structures. • Various correlations were obtained, e.g. correlations between CaCa distances. These relationships can be used directly as spatial restraints. • Restraints and C ...
Higher plant cellulose synthases | Genome Biology | Full Text
... and even some animals. The genes that synthesize cellulose in higher plants differ greatly from the well-characterized genes found in Acetobacter and Agrobacterium sp. More correctly designated as ‘cellulose synthase catalytic subunits’, plant cellulose synthase (CesA) proteins are integral membrane ...
... and even some animals. The genes that synthesize cellulose in higher plants differ greatly from the well-characterized genes found in Acetobacter and Agrobacterium sp. More correctly designated as ‘cellulose synthase catalytic subunits’, plant cellulose synthase (CesA) proteins are integral membrane ...
Proteomic analyses of Oryza sativa mature pollen reveal novel
... the ovule by its polarily growing pollen tube. Yet the molecular genetic basis of these functions is poorly understood. Here, we identified 322 unique proteins, most of which were not reported previously to be in pollen, from mature pollen of Oryza sativa L. ssp japonica using a proteomic approach, ...
... the ovule by its polarily growing pollen tube. Yet the molecular genetic basis of these functions is poorly understood. Here, we identified 322 unique proteins, most of which were not reported previously to be in pollen, from mature pollen of Oryza sativa L. ssp japonica using a proteomic approach, ...
Homology among (βα) 8 Barrels: Implications for the Evolution of
... We provide statistically reliable sequence evidence indicating that at least 12 of 23 SCOP (ba)8 (TIM) barrel superfamilies share a common origin. This includes all but one of the known and predicted TIM barrels found in central metabolism. The statistical evidence is complemented by an examination ...
... We provide statistically reliable sequence evidence indicating that at least 12 of 23 SCOP (ba)8 (TIM) barrel superfamilies share a common origin. This includes all but one of the known and predicted TIM barrels found in central metabolism. The statistical evidence is complemented by an examination ...
Characterization of Acetyl-CoA Carboxylases in the Basal
... the central domain, with no currently defined function [9]. Two different ACC forms exist, a homomeric form found in most eukaryotes and a heteromeric form found in most prokaryotes. The homomeric form exists as one large polypeptide where all the subunits are on one protein with sizes typically in ...
... the central domain, with no currently defined function [9]. Two different ACC forms exist, a homomeric form found in most eukaryotes and a heteromeric form found in most prokaryotes. The homomeric form exists as one large polypeptide where all the subunits are on one protein with sizes typically in ...
vectors
... Four minor coat proteins are found at the tips of the virion, pIII (product of phage gene III) being of particular interest here since it bears foreign amino acids in other phage display vectors. Infection begins with attachment of pIII to an F pilus, the entering ssDNA being converted to double-str ...
... Four minor coat proteins are found at the tips of the virion, pIII (product of phage gene III) being of particular interest here since it bears foreign amino acids in other phage display vectors. Infection begins with attachment of pIII to an F pilus, the entering ssDNA being converted to double-str ...
phylogenetic analysis of pdv movement protein compared to
... through vegetative reproduction. However, the mechanisms of cell-to-cell and systemic transport of the virus are still not known. For the first time this study presents phylogenetic characterization of the movement protein (MP) of PDV isolates from the GenBank database in the context of geographic o ...
... through vegetative reproduction. However, the mechanisms of cell-to-cell and systemic transport of the virus are still not known. For the first time this study presents phylogenetic characterization of the movement protein (MP) of PDV isolates from the GenBank database in the context of geographic o ...
THE INFLUENCE OF BODY MASS INDEX (BMI)
... needs. Circumstances can cause a non-essential amino acid to become essential if the body can no longer produce the required amino acid or not produce sufficient amounts to meet demand, the amino acid becomes conditionally essential. Peptide bonds bind amino acids to one another and can be thought ...
... needs. Circumstances can cause a non-essential amino acid to become essential if the body can no longer produce the required amino acid or not produce sufficient amounts to meet demand, the amino acid becomes conditionally essential. Peptide bonds bind amino acids to one another and can be thought ...
Osmoadaptative Strategy and Its Molecular
... of halophilic proteins possibly allows them to avoid overly rigid folded conformations. On the other hand, the proteomes of halophiles that use organic solutes as their main osmolytes (salt-out organisms) are not enriched in highly acidic proteins, although they typically produce extracellular prote ...
... of halophilic proteins possibly allows them to avoid overly rigid folded conformations. On the other hand, the proteomes of halophiles that use organic solutes as their main osmolytes (salt-out organisms) are not enriched in highly acidic proteins, although they typically produce extracellular prote ...
Translocation Arrest by Reversible Folding of a Precursor Protein
... amino-terminal presequence of the precursor protein, while on the other hand as being exposed to externally added proteases, digesting the part of the precursor which had remained outside the mitochondrion. It was concluded that at sites of import both mitochondrial membranes are sufficiently close ...
... amino-terminal presequence of the precursor protein, while on the other hand as being exposed to externally added proteases, digesting the part of the precursor which had remained outside the mitochondrion. It was concluded that at sites of import both mitochondrial membranes are sufficiently close ...
Stages of Translation (Biol 200 Sp2015): KEY Initiation
... Cell 3: About a third of all new proteins in a mutated cell are not doing their jobs correctly. When you compared to proteins in a healthy cell, these proteins appear much larger overall. Some tRNA has changed it’s anticodon to recognize one of the three STOP codons, so this is erroneously continuin ...
... Cell 3: About a third of all new proteins in a mutated cell are not doing their jobs correctly. When you compared to proteins in a healthy cell, these proteins appear much larger overall. Some tRNA has changed it’s anticodon to recognize one of the three STOP codons, so this is erroneously continuin ...
of membrane lipids
... Protein Motion in Membranes • A variety of protein motions in membranes supports their many functions • Proteins move laterally (through the plane of the membrane) at a rate of a few microns per second • Some integral membrane proteins move more slowly, at diffusion rates of 10 nm per sec – why? • ...
... Protein Motion in Membranes • A variety of protein motions in membranes supports their many functions • Proteins move laterally (through the plane of the membrane) at a rate of a few microns per second • Some integral membrane proteins move more slowly, at diffusion rates of 10 nm per sec – why? • ...
Variation in Glutenin Protein Subunits of Wheat
... bonds, such as the anionic detergent sodium dodecyl sulfate (SOS) (Bietz and Wall 1972). Following these treatments the component subunits can be separated by electrophoresis in polyacrylamide gels containing SOS (SOS-PAGE). However, when SOS-PAGE has been used to examine the glutenin subunit compos ...
... bonds, such as the anionic detergent sodium dodecyl sulfate (SOS) (Bietz and Wall 1972). Following these treatments the component subunits can be separated by electrophoresis in polyacrylamide gels containing SOS (SOS-PAGE). However, when SOS-PAGE has been used to examine the glutenin subunit compos ...
Biochimica et Biophysica Acta
... located close to the translation initiation region. A second plasmid expresses the RNA-binding protein that recognizes this RNA element. When the protein is bound to its cognate RNA located on the reporter mRNA, ribosomal assembly is obstructed, which results in the repression of reporter translatio ...
... located close to the translation initiation region. A second plasmid expresses the RNA-binding protein that recognizes this RNA element. When the protein is bound to its cognate RNA located on the reporter mRNA, ribosomal assembly is obstructed, which results in the repression of reporter translatio ...
No Slide Title
... “Throughout our endeavors we have been motivated by the expectation that the detailed knowledge of its (F0F1 ATP synthase) structure would lead to a better understanding of how ATP is made.” -John Walker Understanding Biology through Structures ...
... “Throughout our endeavors we have been motivated by the expectation that the detailed knowledge of its (F0F1 ATP synthase) structure would lead to a better understanding of how ATP is made.” -John Walker Understanding Biology through Structures ...
Influence of Valine Analogues on Protein Synthesis of Chicken
... Valine and its analogues (D-valine, methylvaline, valinol) were supplemented into Medium 199 containing 20 ng/ml of chicken insulin-like growth factor-I (IGF-I) instead of fetal calf serum (FCS). Influence of branched chain amino acids (isoleucine, leucine, valine) on myoblast protein synthesis was ...
... Valine and its analogues (D-valine, methylvaline, valinol) were supplemented into Medium 199 containing 20 ng/ml of chicken insulin-like growth factor-I (IGF-I) instead of fetal calf serum (FCS). Influence of branched chain amino acids (isoleucine, leucine, valine) on myoblast protein synthesis was ...
Interactome
In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.