Protein sequence databases
... one of the oldest in computational biology. Back in the 60s, when the first protein sequence database was published as printed version, Margaret Dayhoff defined the basic principles of this discipline with only a small number of sequences at hand. Nowadays, with more than a million protein sequences ...
... one of the oldest in computational biology. Back in the 60s, when the first protein sequence database was published as printed version, Margaret Dayhoff defined the basic principles of this discipline with only a small number of sequences at hand. Nowadays, with more than a million protein sequences ...
A Guide to the Analysis and Purification of Proteins and
... protein therapeutic products and to analyze these for product identity and impurities. Reversed-phase HPLC plays a vital role in the separation of peptides from digested proteomes prior to protein identification by mass spectrometry. It is also used to purify many proteins and peptides during invest ...
... protein therapeutic products and to analyze these for product identity and impurities. Reversed-phase HPLC plays a vital role in the separation of peptides from digested proteomes prior to protein identification by mass spectrometry. It is also used to purify many proteins and peptides during invest ...
Protein Folding and Expression
... Examples of successfully expressed proteins can be seen in Table 1. This includes expression of enzymes, antigens, and cytokines. Each protein was produced at a very high level of expression and confirmed to have native biological activity. In addition, proteins from taxonomically distant organisms ...
... Examples of successfully expressed proteins can be seen in Table 1. This includes expression of enzymes, antigens, and cytokines. Each protein was produced at a very high level of expression and confirmed to have native biological activity. In addition, proteins from taxonomically distant organisms ...
Intersubunit contacts are often facilitated by specificity
... recognition of the interaction partner ...
... recognition of the interaction partner ...
and paralogue-specific functions of acyl-CoA
... accommodate binding of fatty acyl-CoA with high affinity (K d is approximately 1 nM) [17]. Although the basal ACBP displays the hallmarks of a housekeeping gene in mammals [18] and hence has been proposed to serve a basal function common to all cells, its precise cellular role is yet to be elucidate ...
... accommodate binding of fatty acyl-CoA with high affinity (K d is approximately 1 nM) [17]. Although the basal ACBP displays the hallmarks of a housekeeping gene in mammals [18] and hence has been proposed to serve a basal function common to all cells, its precise cellular role is yet to be elucidate ...
Protein sequence analysis
... proteins. It produces biologically meaningful multiple sequence alignments of divergent sequences. It calculates the best match for the selected sequences, and lines them up so that the identities, similarities and differences can be seen. Evolutionary relationships can be seen through Cladograms or ...
... proteins. It produces biologically meaningful multiple sequence alignments of divergent sequences. It calculates the best match for the selected sequences, and lines them up so that the identities, similarities and differences can be seen. Evolutionary relationships can be seen through Cladograms or ...
Differential display proteomic analysis of Picea meyeri pollen
... angiosperms develop and elongate via different mechanisms. Because many proteins associated with the actin cytoskeleton are probably involved in the signalling pathways that control pollen development (Gibbon et al., 1999; Vidali et al., 2001), a proteomic investigation could help elucidate the sign ...
... angiosperms develop and elongate via different mechanisms. Because many proteins associated with the actin cytoskeleton are probably involved in the signalling pathways that control pollen development (Gibbon et al., 1999; Vidali et al., 2001), a proteomic investigation could help elucidate the sign ...
HIS-Select Nickel Affinity Gel (P6611) - Technical - Sigma
... may be extracted from a crude cell extract or a partially purified protein fraction prepared by standard techniques. The protein sample preparation steps should be empirically determined by the end user since the conditions may vary depending on the nature of the recombinant protein and the host org ...
... may be extracted from a crude cell extract or a partially purified protein fraction prepared by standard techniques. The protein sample preparation steps should be empirically determined by the end user since the conditions may vary depending on the nature of the recombinant protein and the host org ...
1 Chapter 1 Introduction 1.1 – Introduction Proteins are
... selenium and tellurium have been developed for incorporation into proteins. Importantly, these amino acid derivatives are recognized by the protein synthesis machinery and can, therefore, be incorporated in vivo. These heavy atoms allow crystallographers to more easily locate discrete positions in a ...
... selenium and tellurium have been developed for incorporation into proteins. Importantly, these amino acid derivatives are recognized by the protein synthesis machinery and can, therefore, be incorporated in vivo. These heavy atoms allow crystallographers to more easily locate discrete positions in a ...
Presentation Slides - Society of Barley Engineers
... concentration and composition Two bottom-fermenting lager strains (Guinness 6701 and 7012) in 2 l 15 °P wort in European Brewery Convention (EBC) tall cylindroconical Dynamics of brewing fermentation Transcriptome (microarray, S.c. gene probeset) ...
... concentration and composition Two bottom-fermenting lager strains (Guinness 6701 and 7012) in 2 l 15 °P wort in European Brewery Convention (EBC) tall cylindroconical Dynamics of brewing fermentation Transcriptome (microarray, S.c. gene probeset) ...
Designed sequences improve distant homology detection
... sequence (e-value cutoff 0.01). We obtain a position specific scoring matrix (PSSM) using –Q option in the program BLASTPGP (13). This PSSM is used for deriving the single residue potential. We obtain a multiple alignment of found homologs directly from the output of PSI-BLAST. Seventy sequences are ...
... sequence (e-value cutoff 0.01). We obtain a position specific scoring matrix (PSSM) using –Q option in the program BLASTPGP (13). This PSSM is used for deriving the single residue potential. We obtain a multiple alignment of found homologs directly from the output of PSI-BLAST. Seventy sequences are ...
The Early Interaction of the Outer Membrane Protein PhoE with
... MC4100 (F⫺ ⌬lacU169 araD136 rpsL thi relA) was used for the spheroplast labeling experiments and for the isolation of lysate and inner membrane vesicles (19). MC4100 and RC354c, containing a deletion in the skp gene (17), were used for the isolation of periplasmic extracts (20). BG87 (21) was used f ...
... MC4100 (F⫺ ⌬lacU169 araD136 rpsL thi relA) was used for the spheroplast labeling experiments and for the isolation of lysate and inner membrane vesicles (19). MC4100 and RC354c, containing a deletion in the skp gene (17), were used for the isolation of periplasmic extracts (20). BG87 (21) was used f ...
Cysteine 230 Modulates Tumor Necrosis Factor
... exhibits much stronger apoptotic activity than other TNF family members and is able to kill many cell types without potentiating agents such as actinomycin D or cycloheximide (10, 18, 19). Second, TRAIL kills tumor cells more effectively than normal cells by virtue of differential expression of DcRs ...
... exhibits much stronger apoptotic activity than other TNF family members and is able to kill many cell types without potentiating agents such as actinomycin D or cycloheximide (10, 18, 19). Second, TRAIL kills tumor cells more effectively than normal cells by virtue of differential expression of DcRs ...
Denaturation of hemoglobin in the presence of tannic acid
... Tannic acid (TA) is a natural gallotannin and is comprised of a pentagalloylglucose core esterified at all functional hydroxyl groups with gallic acid molecules (see Scheme) [4]. TA belongs to the class of hydrolysable tannins and is found in numerous plants and foods. It generally can be extracted ...
... Tannic acid (TA) is a natural gallotannin and is comprised of a pentagalloylglucose core esterified at all functional hydroxyl groups with gallic acid molecules (see Scheme) [4]. TA belongs to the class of hydrolysable tannins and is found in numerous plants and foods. It generally can be extracted ...
Metabolism II
... of soluble protein in a solution. - It is used in the detection and estimation of proteins and peptides. - The reaction is characterized by a blue-violet color upon the addition of cupper sulfate to any compound containing more than 2 peptide bonds. - Biuret reagent (dilute copper sulphate in strong ...
... of soluble protein in a solution. - It is used in the detection and estimation of proteins and peptides. - The reaction is characterized by a blue-violet color upon the addition of cupper sulfate to any compound containing more than 2 peptide bonds. - Biuret reagent (dilute copper sulphate in strong ...
Modulating the cobalt redox potential through imidazole hydrogen
... Furthermore, comparison with the active site of a “His-on” bound B12-cofactor from crystal structure indicates a good agreement with the structural behavior of 1-H+ (Fig. 2). Reversible proton release of 1-H+ (Scheme 1) was then tested by pH titration (from pH 8.5 to pH 12.5) and was detected with U ...
... Furthermore, comparison with the active site of a “His-on” bound B12-cofactor from crystal structure indicates a good agreement with the structural behavior of 1-H+ (Fig. 2). Reversible proton release of 1-H+ (Scheme 1) was then tested by pH titration (from pH 8.5 to pH 12.5) and was detected with U ...
Substrate specificity of protein kinase A in reaction with
... 2002). The phosphorylation modulates the activity of many proteins and protein kinases play a key role in multiple signaling and regulatory phenomena in cell. It has been estimated that as much as 20-50% of all the cellular proteins undergo phosphorylation in vivo (Pinna and Ruzzene, 1996) .On the o ...
... 2002). The phosphorylation modulates the activity of many proteins and protein kinases play a key role in multiple signaling and regulatory phenomena in cell. It has been estimated that as much as 20-50% of all the cellular proteins undergo phosphorylation in vivo (Pinna and Ruzzene, 1996) .On the o ...
Recombinant N-terminal Nucleotide
... product CFTR, to bacterial transporters (10). The ATPase activity and related drug transport of P-glycoprotein require both functional nucleotide-binding sites (11, 12) and are sensitive to the cysteine-specific modifier N-ethylmaleimide (NEM) (13– 17). The lack of structural data about P-glycoprote ...
... product CFTR, to bacterial transporters (10). The ATPase activity and related drug transport of P-glycoprotein require both functional nucleotide-binding sites (11, 12) and are sensitive to the cysteine-specific modifier N-ethylmaleimide (NEM) (13– 17). The lack of structural data about P-glycoprote ...
soy protein concentrate - US Soybean Export Council
... selected and cleaned as the first step in soy product processing. The clean soybeans are dehulled, then the oil is extracted for cooking, biodiesel and other uses. The residue, consisting of defatted white flakes, can then be ground to make soy flour, toasted and ground to make soybean meal, or subj ...
... selected and cleaned as the first step in soy product processing. The clean soybeans are dehulled, then the oil is extracted for cooking, biodiesel and other uses. The residue, consisting of defatted white flakes, can then be ground to make soy flour, toasted and ground to make soybean meal, or subj ...
Interactome
In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.