amino acids
... yields without the bitter β-form byproduct. • may hydrolyze into its constituent amino acids under conditions of elevated temperature or high pH. At room temperature, it is most stable at pH 4.3, where its half-life is nearly 300 days. At pH 7, however, its half-life is only a few days ...
... yields without the bitter β-form byproduct. • may hydrolyze into its constituent amino acids under conditions of elevated temperature or high pH. At room temperature, it is most stable at pH 4.3, where its half-life is nearly 300 days. At pH 7, however, its half-life is only a few days ...
Directed mutagenesis of the Trypanosoma cruzi trans
... 4—6 and the flanking C-terminal region after the T.rangeli sialidase ^-sheets 1-3 of pG5EX, encoded an enzymatically inactive product (data not shown). The next hybrid construct, pS3—2, inserted all of P-sheets 4 and 5 and the first strand of sheet 6 from T. rangeli sialidase in place of the TS sequ ...
... 4—6 and the flanking C-terminal region after the T.rangeli sialidase ^-sheets 1-3 of pG5EX, encoded an enzymatically inactive product (data not shown). The next hybrid construct, pS3—2, inserted all of P-sheets 4 and 5 and the first strand of sheet 6 from T. rangeli sialidase in place of the TS sequ ...
A Study of the Membrane–Water Interface Region of Membrane
... from the center of the membrane, while apolar residues tend to point towards the center.15 We have carried out a similar analysis for the interfacial secondary structure elements by calculating the angle f of the Ca–Cb bond relative to the membrane normal for each residue in the interface region. Re ...
... from the center of the membrane, while apolar residues tend to point towards the center.15 We have carried out a similar analysis for the interfacial secondary structure elements by calculating the angle f of the Ca–Cb bond relative to the membrane normal for each residue in the interface region. Re ...
Structural Insights into Catalysis and Inhibition of O
... Gene Cloning and Protein Production—The DNA sequence coding for CysK1 from M. tuberculosis (Rv2334) was amplified by PCR and cloned using upstream NdeI and SCHEME 1. Schematic representation of the reaction catalyzed by CysK1. downstream HindIII restriction sites. Subsequently, the sequence enzymes ...
... Gene Cloning and Protein Production—The DNA sequence coding for CysK1 from M. tuberculosis (Rv2334) was amplified by PCR and cloned using upstream NdeI and SCHEME 1. Schematic representation of the reaction catalyzed by CysK1. downstream HindIII restriction sites. Subsequently, the sequence enzymes ...
Key Words
... Objective: Understand the process of Translation Key Words: translation, codon, anticodon, mRNA, polypeptide Arrange the following sentences in order to describe translation from mRNA to proteins A. A second tRNA links to a second codon in the mRNA ...
... Objective: Understand the process of Translation Key Words: translation, codon, anticodon, mRNA, polypeptide Arrange the following sentences in order to describe translation from mRNA to proteins A. A second tRNA links to a second codon in the mRNA ...
Non-Essential Amino Acids
... oxidation. Overweight patients generally have lower STH concentrations, which often hinders weight reduction.1 Unfortunately, the growth hormone is very expensive (approximately GBP 400–650 for a monthly ration) and must be injected under close and competent medical supervision. It is thus safer to ...
... oxidation. Overweight patients generally have lower STH concentrations, which often hinders weight reduction.1 Unfortunately, the growth hormone is very expensive (approximately GBP 400–650 for a monthly ration) and must be injected under close and competent medical supervision. It is thus safer to ...
Tendency for Local Repetitiveness in Amino Acid Usages in Modern
... Systematic analyses of human proteins show that neural and immune system-speci®c, and therefore, relatively ``modern'' proteins have a tendency for repetitive use of amino acids at a local scale (1-20 residues), while ancient proteins (human homologues of Escherichia coli proteins) do not. Those pr ...
... Systematic analyses of human proteins show that neural and immune system-speci®c, and therefore, relatively ``modern'' proteins have a tendency for repetitive use of amino acids at a local scale (1-20 residues), while ancient proteins (human homologues of Escherichia coli proteins) do not. Those pr ...
Predicting the Secondary Structure of Globular Proteins Using
... how to predict the secondary structure of local sequences of amino acids. The average success rate of our method on a testing set of proteins non-homologous with the corresponding training set was 643% on three types of secondary structure (u-helix, b-sheet, and coil), with correlation coefficients ...
... how to predict the secondary structure of local sequences of amino acids. The average success rate of our method on a testing set of proteins non-homologous with the corresponding training set was 643% on three types of secondary structure (u-helix, b-sheet, and coil), with correlation coefficients ...
Citrate synthase proteins in extremophilic organisms: Studies within
... from a mesophilic organism; three are in the closed and two in the open conformations. Even though the proteins belong to the same fold, the model distinguishes the properties of these proteins in a way which is consistent with experiments. For instance, the thermophilic proteins are more stable the ...
... from a mesophilic organism; three are in the closed and two in the open conformations. Even though the proteins belong to the same fold, the model distinguishes the properties of these proteins in a way which is consistent with experiments. For instance, the thermophilic proteins are more stable the ...
Isolation of AOXI promoter
... the blood alcohol concentration of an individual. It is constructed using DNA from Pichia pastoris, a strain of yeast with a diauxic metabolic pathway for ethanol and methanol. The alcohol sensor will utilize this metabolic activity, along with a fluorescent protein indicator fused with the alcohol ...
... the blood alcohol concentration of an individual. It is constructed using DNA from Pichia pastoris, a strain of yeast with a diauxic metabolic pathway for ethanol and methanol. The alcohol sensor will utilize this metabolic activity, along with a fluorescent protein indicator fused with the alcohol ...
BI25M1
... Cyanobacteria have specialised N-fixing cells (‘heterocysts’) with O2-inpenetrable walls; legumes produce leghaemoglobin, which, like RBC haemoglobin, binds O2 (and prevents the inhibition). ...
... Cyanobacteria have specialised N-fixing cells (‘heterocysts’) with O2-inpenetrable walls; legumes produce leghaemoglobin, which, like RBC haemoglobin, binds O2 (and prevents the inhibition). ...
Canine CD34: Cloning of the cDNA and Evaluation
... rapidly through graded alcohols. As negative controls, staining was performed on canine tissues without primary antibody on andhuman lung to assess nonspecific tissue reactivity of RPacaCD34. A rabbit polyclonal antihuman factor VI11 (Dako, Carpinteria. CA) was used as a positive control to identify ...
... rapidly through graded alcohols. As negative controls, staining was performed on canine tissues without primary antibody on andhuman lung to assess nonspecific tissue reactivity of RPacaCD34. A rabbit polyclonal antihuman factor VI11 (Dako, Carpinteria. CA) was used as a positive control to identify ...
... DNA was measured as a function of salt concentration and the binding curves are shown on the right. Based on these data, what type(s) of interactions are used by the lac repressor to bind to DNA [Hint: It may be useful to sketch a plot of KD versus [NaCl]] The Kd increases as salt increases, indicat ...
Structure and function of human lactalbumin made lethal to tumor
... field has widened in scope, acquiring new members and enriching our understanding of the basic principles underlying protein self-assembly and acquisition of new functionality. HAMLET key features are related to the intrinsic properties of proteins to possess varying functions depending on their conf ...
... field has widened in scope, acquiring new members and enriching our understanding of the basic principles underlying protein self-assembly and acquisition of new functionality. HAMLET key features are related to the intrinsic properties of proteins to possess varying functions depending on their conf ...
1 Engineering Lipases with an Expanded Genetic Code - Wiley-VCH
... properties through protein engineering strategies [6]. Protein engineers focus not only on increasing the enzyme stability but, given the high versatility of lipases, also on the improvement of promiscuous catalytic activities toward nonnatural substrates, for example, usage of the other enantiomer ...
... properties through protein engineering strategies [6]. Protein engineers focus not only on increasing the enzyme stability but, given the high versatility of lipases, also on the improvement of promiscuous catalytic activities toward nonnatural substrates, for example, usage of the other enantiomer ...
Cloning of Plastid Acetyl-CoA Carboxylase cDNA from Setaria italica
... from plastid and cytosol, which have Leu and Ile respectively at the herbicide binding site. The result showed that their molecular masses, native conformations and Michaelis constants for three substrates were all rather similar. Moreover, the reaction characteristics were close to an ordered mecha ...
... from plastid and cytosol, which have Leu and Ile respectively at the herbicide binding site. The result showed that their molecular masses, native conformations and Michaelis constants for three substrates were all rather similar. Moreover, the reaction characteristics were close to an ordered mecha ...
Feed Ingredients and Feeds for Channel Catfish
... obtained by grinding the flakes remaining after removing most of the oil from dehulled soybeans by a solventextraction process. It contains about 48 percent highquality protein and is the predominant protein source in catfish feeds. Soybean meal has the best amino acid profile of all common plant pr ...
... obtained by grinding the flakes remaining after removing most of the oil from dehulled soybeans by a solventextraction process. It contains about 48 percent highquality protein and is the predominant protein source in catfish feeds. Soybean meal has the best amino acid profile of all common plant pr ...
PDF of article
... The unusually high Matthews coefficient (VM = 9.17 Å3 Da1) and high solvent content (>80%; Matthews, 1968) of the cubic crystals leads to the presence of large solvent channels. In contrast, most GlmU proteins crystallize in the H3/H32/P6322 form and do not show the presence of such large channels ...
... The unusually high Matthews coefficient (VM = 9.17 Å3 Da1) and high solvent content (>80%; Matthews, 1968) of the cubic crystals leads to the presence of large solvent channels. In contrast, most GlmU proteins crystallize in the H3/H32/P6322 form and do not show the presence of such large channels ...
(9E10): sc-40 - Santa Cruz Biotechnology
... provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions w ...
... provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions w ...
33_organelles.txt 3/25/2010 Limited proteolysis, phosphorylation
... Since their DNA copying does not have any error checking, the probability of random mutation is much higher, leading to diseases such as Kearn-Sayre syndrome. Its DNA typically consists of five to ten copies, all inherited from the mother, and when they divide these copies are randomly distributed b ...
... Since their DNA copying does not have any error checking, the probability of random mutation is much higher, leading to diseases such as Kearn-Sayre syndrome. Its DNA typically consists of five to ten copies, all inherited from the mother, and when they divide these copies are randomly distributed b ...
Candidate genes and single nucleotide polymorphisms associated
... (Hubbard et al., 2009) were used as reference sequences. The SNP returned by the Maq SNPfilter command were annotated using a collection of command-line scripts (Grant et al., 2011) NGS-SNP (Grant et al., 2011) by assigning a functional class to each SNP (e.g., nonsynonymous) and then providing Ense ...
... (Hubbard et al., 2009) were used as reference sequences. The SNP returned by the Maq SNPfilter command were annotated using a collection of command-line scripts (Grant et al., 2011) NGS-SNP (Grant et al., 2011) by assigning a functional class to each SNP (e.g., nonsynonymous) and then providing Ense ...
Development of Biocatalysts for Production of Fine Chemicals
... pharmaceuticals and agrochemicals. The principal advantage of biocatalysts is their ability to catalyze reactions with high specificity (often enantio- or regio-selectively). Furthermore, biocatalysts have the advantage of operating under mild conditions, typically at ambient temperature and pressur ...
... pharmaceuticals and agrochemicals. The principal advantage of biocatalysts is their ability to catalyze reactions with high specificity (often enantio- or regio-selectively). Furthermore, biocatalysts have the advantage of operating under mild conditions, typically at ambient temperature and pressur ...
12. Molecular Recognition: The Thermodynamics of
... In Chapter 11 we introduced the concept of the equilibrium constant, K, which governs the concentrations of reactants and products in a reaction that has reached equilibrium. In this chapter and the next one we focus on the analysis of equilibrium constants for a particularly important subset of mol ...
... In Chapter 11 we introduced the concept of the equilibrium constant, K, which governs the concentrations of reactants and products in a reaction that has reached equilibrium. In this chapter and the next one we focus on the analysis of equilibrium constants for a particularly important subset of mol ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.