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Slides PPT
Slides PPT

... • The lac operon is under two forms of control; positive and negative control. • Negative control occurs when the binding of a protein prevents an event. The brake on the car • Positive control is when the binding causes the event. The accelerator on the ...
Amino Acid Metabolism
Amino Acid Metabolism

... Metabolic Pool of Amino Acids • Metabolic pool AA has no storage form in mammals (as with other life forms) as free AA or as specialized storage form (such as glycogen for glucose, TG for FA) but a certain percentage of muscle & structural proteins are “expendable”. • AA are used for proteins, N co ...
Allosteric enzymes
Allosteric enzymes

... Allosteric enzymes often cooperative S binding Allosteric activators and inhibitors: • Bind at allosteric site, not catalytic site • Conformational change ...
1 Amino Acid Metabolism
1 Amino Acid Metabolism

... Metabolic Pool of Amino Acids • Metabolic pool AA has no storage form in mammals (as with other life forms) as free AA or as specialized storage form (such as glycogen for glucose, TG for FA) but a certain percentage of muscle & structural proteins are “expendable”. • AA are used for proteins, N co ...
Electron Transport Chain (1)
Electron Transport Chain (1)

... - H+ ions enter binding sites within a rotor, changing the shape of each subunit so that the rotor spins within the membrane - Each H+ ion makes one complete turn, leaving the rotor and passing through a second half channel in the stator into the mitochondrial matrix - Spinning of the rotor causes a ...
pTcGW platform guideline Gateway® cloning system: general
pTcGW platform guideline Gateway® cloning system: general

... vector. pTcGW plasmids are destination vectors, possessing the Gateway cassette RfA, including attR1 and attR2 sites, chloramphenicol resistance and ccdB gene. The attB1 and attB2 sites are added to the insert by polymerase chain reaction (PCR) amplification, then in the presence of BP Clonase mix, ...
Biomolecule Review Worksheet
Biomolecule Review Worksheet

... group. Some “R” groups are very small, others are large, and even others form chains and rings. The sequence and shapes of the “R” groups control the shape and function of the protein. 4. How many different amino acids are there? 5. What part of the amino acid varies from one amino acid to another? ...
Extension Article for students who scored 85 or above
Extension Article for students who scored 85 or above

... chemical compound. Molecules can be made of single types of atoms or of different types. For example, the oxygen in the air is made of two oxygen atoms (O2), but water is made of two hydrogen atoms and one oxygen atom (H2O). ...
Searching for frameshift evolutionary relationships between protein
Searching for frameshift evolutionary relationships between protein

... deletions. Paralogs, which are similar proteins in the same organism, diverged by similar mechanisms after gene duplication events. Evolutionary connections are established among the known proteins by statistical methods of sequence comparison. Typically, dynamic programming algorithms are used to p ...
Computational method on biochemistry
Computational method on biochemistry

... 으로 score 설정 • matrices are based on global alignments of closely related proteins. The PAM 1 is the matrix calculated from comparisons of sequences with no more than 1% divergence. Scores are derived from a mutation probability matrix where each element gives the probability of the amino acid in col ...
Summary
Summary

... steady state had been reached, the cultures were shifted to nitrogen-starvation conditions, in the presence of an excess of glucose to prevent dual starvation. Simultaneously, the cultures were brought to batch conditions, since the cells would not grow when starved for nitrogen. The fluxes through ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)

... for evaluation to check the quality of the generated model. There was more than 62 % sequence identity with E value 0.00e-1 in query and template sequences. Then MODELLER was used to generate the three dimensional structure of PEPCK. It is found that template structure contains different ligand whic ...
A novel sensitive method for the detection of user
A novel sensitive method for the detection of user

... Identification of compositionally biased segments is important not only in sequence similarity searches. DNA sequences contain a large fraction of diverse biased segments with poorly understood function. More than 50% of all proteins have been shown to contain biased segments (Wootton and Federhen, ...
Is DNA the Genetic Material?
Is DNA the Genetic Material?

... Hypothetically, which of the following molecules could work as a replacement for tRNA? 1)  A protein that can bind to the major groove of DNA and a specific amino acid. 2)  A carbohydrate that can recognize a short RNA sequence and bind to a particular amino acid. 3)  A lipid that can basepair with ...
Journal of Biotechnology VI-2 Genomics, proteomics and
Journal of Biotechnology VI-2 Genomics, proteomics and

... solar saltern lake in Egypt. Strain EG6 can grow in a wide range of NaCl concentrations up to 4 M. Phylogenetic position was established by 16S rRNA gene sequencing as a member of the genus Halomonas. Morphological, physiological and biochemical tests were done to characterize strain EG6 Leifson (19 ...
Example of identifying a monogenic condition by positional cloning
Example of identifying a monogenic condition by positional cloning

... Channel lining is formed by M1, M3, M6 & M12 segments. ...
a. carbohydrates - Valhalla High School
a. carbohydrates - Valhalla High School

... • DNA is the hereditary material passed from one generation to the next during reproduction. ...
Gene Section ALOX5 (arachidonate 5-lipoxygenase) Atlas of Genetics and Cytogenetics
Gene Section ALOX5 (arachidonate 5-lipoxygenase) Atlas of Genetics and Cytogenetics

... Online updated version: http://AtlasGeneticsOncology.org/Genes/ALOX5ID42985ch10q11.html DOI: 10.4267/2042/38350 This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. © 2006 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
Enzymes..
Enzymes..

... Find the second parts of expressions about proteins There are two types of the secondary protein structure ….. A. an alpha helix and the beta pleated sheet B. are between 5500 and 220,000 C. is a polypeptide chain D. caused by hydrogen bonds E. its three-dimensional structure Find the second parts o ...
Carbon Compounds slideshow Carbon Compounds
Carbon Compounds slideshow Carbon Compounds

... • Compound – Pure substance made of two or more elements, e.g. water (H2O) carbon dioxide (CO2) ______________ _______________________ atoms • Molecule – Particle made of two or more __________ joined together. compound Smallest particle of a __________________ ...
R N A & PROTEIN SYNTHESIS
R N A & PROTEIN SYNTHESIS

... The function of normal human red blood cells, which are disk-shaped, is to transport oxygen from the lungs to the other organs of the body. Each red blood cell contains millions of molecules of hemoglobin that carries the ...
The Structure and Topology of Protein Serine/Threonine
The Structure and Topology of Protein Serine/Threonine

Macromolecules of Life
Macromolecules of Life

... • A denatured protein is biologically inactive Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings ...
The relative importance of intracellular proteolysis and
The relative importance of intracellular proteolysis and

... required are hydrolyzed in energy– dependent reactions catalyzed by intracellular proteinases [2]. Cells contain a large number of endopeptidases that are localized in the cytoplasm, periplasm and cytoplasmic membrane [3,4]. In addition to endopeptidases, exopeptidases further degrade the peptides g ...
CH 15 PowerPoint
CH 15 PowerPoint

... group of one molecule reacts with the amine group of the other molecule, thereby releasing a molecule of water. This is a dehydration synthesis reaction (also known as a condensation reaction), and usually occurs between amino acids. The resulting C(O)NH bond is called a peptide bond, and the result ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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