Fragmenta Agronomica 2
... – year of the experiment. The content of isoleucine, on the other hand, was significantly lower. In the case of endogenous amino acids, in the second year of the study their total content was 59.39 g·100 g-1 of protein, and was significantly higher compared to the first year, by 3.15 g, i.e. by 5.6% ...
... – year of the experiment. The content of isoleucine, on the other hand, was significantly lower. In the case of endogenous amino acids, in the second year of the study their total content was 59.39 g·100 g-1 of protein, and was significantly higher compared to the first year, by 3.15 g, i.e. by 5.6% ...
Structure of DIG
... Detection by chemiluminescence: a complicated chain of events First: Incorporate “DIG” (Digoxigenin) into your probe DNA Structure of DIG: ...
... Detection by chemiluminescence: a complicated chain of events First: Incorporate “DIG” (Digoxigenin) into your probe DNA Structure of DIG: ...
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... process takes just 25 milliseconds. Enzyme rates depend on solution conditions and substrate concentration. Conditions that denature the protein abolish enzyme activity, such as high temperatures, extremes of pH or high salt concentrations, while raising substrate concentration tends to increase act ...
... process takes just 25 milliseconds. Enzyme rates depend on solution conditions and substrate concentration. Conditions that denature the protein abolish enzyme activity, such as high temperatures, extremes of pH or high salt concentrations, while raising substrate concentration tends to increase act ...
Guidelines for separating DNA (Deoxyribonucleic Acid) using gel
... material that will support the DNA or protein to be analysed. The purpose of using any matrix in gel electrophoresis is to prevent convective mixing that can be caused by heating. These support matrices can be stained using safe dyes such as methylene blue, (but the resolution may not be sufficientl ...
... material that will support the DNA or protein to be analysed. The purpose of using any matrix in gel electrophoresis is to prevent convective mixing that can be caused by heating. These support matrices can be stained using safe dyes such as methylene blue, (but the resolution may not be sufficientl ...
Southern molecular hybridization experiments with parallel
... locus of D. melanogaster [14] were used as the model for studying the hybridization of Southern filters with the parallel complementary probe (probe A). The same blot was hybridized in 2x SSC solution at 320C with the parallel probe and then at 54°C with the antiparallel one. Fig. 2 shows the result ...
... locus of D. melanogaster [14] were used as the model for studying the hybridization of Southern filters with the parallel complementary probe (probe A). The same blot was hybridized in 2x SSC solution at 320C with the parallel probe and then at 54°C with the antiparallel one. Fig. 2 shows the result ...
RED CELL MEMBRANE DEFECTS
... exposure to certain oxidants. This leads to production of reduced glutathione to protect the RBCs. ...
... exposure to certain oxidants. This leads to production of reduced glutathione to protect the RBCs. ...
MD Simulations of the P53 oncoprotein structure
... to DNA is separately displayed. Considering the above noted difficulty of zinc binding in p53c, experiments point to the importance of zinc coordination for achieving the correct folding and correct binding of p53 to a specific DNA in intact cells. In our simulation, however, both non-bonded and bon ...
... to DNA is separately displayed. Considering the above noted difficulty of zinc binding in p53c, experiments point to the importance of zinc coordination for achieving the correct folding and correct binding of p53 to a specific DNA in intact cells. In our simulation, however, both non-bonded and bon ...
Authors Title Year Keywords Journal/Proceedings Emile Bol
... with the addition of 1.0 mM dipicolinic acid for the reduction of [Fe(bpy)2(CN)2]+ and is completely suppressed with the addition of 5.0 mM EDTA (pH < 9.00), 10.0 mM EDTA (9.0 < pH 10.0), and 1.0 mM cyclam (pH > 10.0) for the reduction of [Fe(bpy)(CN)4]-. 1H NMR and UV-vis spectra show that the prod ...
... with the addition of 1.0 mM dipicolinic acid for the reduction of [Fe(bpy)2(CN)2]+ and is completely suppressed with the addition of 5.0 mM EDTA (pH < 9.00), 10.0 mM EDTA (9.0 < pH 10.0), and 1.0 mM cyclam (pH > 10.0) for the reduction of [Fe(bpy)(CN)4]-. 1H NMR and UV-vis spectra show that the prod ...
Protein degradation in mitochondria
... removed upon assembly of PIM1 subunits. Substrates of Lon-like proteases in mitochondria have only been identified in yeast and include various non-assembled polypeptides, such as β-MPP, subunits α, β and γ of the F1 F0 -ATP synthase and ribosomal proteins,20 as well as missorted and misfolded model ...
... removed upon assembly of PIM1 subunits. Substrates of Lon-like proteases in mitochondria have only been identified in yeast and include various non-assembled polypeptides, such as β-MPP, subunits α, β and γ of the F1 F0 -ATP synthase and ribosomal proteins,20 as well as missorted and misfolded model ...
... is similar to that of the two acyl chains, thus they pack to form an extended two-dimensional array of molecules. C. The CMC is the highest concentration of free fatty acids that can be obtained in solution before micelles form (+4 pts). Palmitic acid is more non-polar than butyric acid, so it will ...
The Plasma Membrane - Beck-Shop
... Membrane–DNA Interactions: More Questions than Answers With the absence of internal membranes in prokaryotes, scientists have often speculated that the plasma membrane may function in directing some of the cellular processes. In 1963, F. Jacob and colleagues proposed the Replicon Model in which the ...
... Membrane–DNA Interactions: More Questions than Answers With the absence of internal membranes in prokaryotes, scientists have often speculated that the plasma membrane may function in directing some of the cellular processes. In 1963, F. Jacob and colleagues proposed the Replicon Model in which the ...
L-ASPG86 - Journal of Microbiology and Biotechnology
... product and pET-16b vector (Novagen, USA) were digested with NdeI (Takara, Japan) and XhoI (Takara) restriction enzymes, and ligated with T4 DNA ligase (Takara). The ligation product was transformed into E. coli DH5α cells and purified with the AccuPrep Nano-Plus Plasmid Mini Extraction Kit (Bioneer ...
... product and pET-16b vector (Novagen, USA) were digested with NdeI (Takara, Japan) and XhoI (Takara) restriction enzymes, and ligated with T4 DNA ligase (Takara). The ligation product was transformed into E. coli DH5α cells and purified with the AccuPrep Nano-Plus Plasmid Mini Extraction Kit (Bioneer ...
A first attempt to elucidate the amino acid sequence of some lichen
... mixed with 75 μl glycerol. This solution was applied onto 12% polyacrylamide gels, and subjected to standard electrophoresis, employing 50 mM Tris-glycine (pH 8.3) as running buffer. The mixture SeeBlue 2 plus (Invitrogen, Carlsbad, CA, USA) was used as molecular mass marker. SDS-PAGE Both proteins ...
... mixed with 75 μl glycerol. This solution was applied onto 12% polyacrylamide gels, and subjected to standard electrophoresis, employing 50 mM Tris-glycine (pH 8.3) as running buffer. The mixture SeeBlue 2 plus (Invitrogen, Carlsbad, CA, USA) was used as molecular mass marker. SDS-PAGE Both proteins ...
Comparison of the Structure of the Extrinsic 33 kDa Protein from
... 33 kDa protein was cleaved at 16Y by chymotrypsin and at 18E by V8 protease (Eaton-Rye and Murata 1989), while the cyanobacterial 33 kDa protein was cleaved primarily at 156F and 190F by chymotrypsin (Motoki et al. 1998). Another line of evidence suggesting a possible difference in the structure of ...
... 33 kDa protein was cleaved at 16Y by chymotrypsin and at 18E by V8 protease (Eaton-Rye and Murata 1989), while the cyanobacterial 33 kDa protein was cleaved primarily at 156F and 190F by chymotrypsin (Motoki et al. 1998). Another line of evidence suggesting a possible difference in the structure of ...
... Choice A: Why are weak acids buffers within one pH unit of their pKa? Choice B: Briefly describe how to make a buffer solution, given that the pH of the solution should be equal to the pKa of a monoprotic buffer. Choice A: If base is added then the weak acid dissociates to neutralize the base. If ac ...
Cis-trans isomerization of omega dihedrals in Proteins
... (ψ) around the Cα-C bond, and the omega-angle (ω) around the C-N bond – peptide bond. The φ and ψ-angle define the structure of a protein backbone. Due to the delocalization of the carbonyl π electrons and the nitrogen lone pair, the ω angle tends to be planar and therefore is very rigid. From a the ...
... (ψ) around the Cα-C bond, and the omega-angle (ω) around the C-N bond – peptide bond. The φ and ψ-angle define the structure of a protein backbone. Due to the delocalization of the carbonyl π electrons and the nitrogen lone pair, the ω angle tends to be planar and therefore is very rigid. From a the ...
Biomolecular chemistry 3. Translating the genetic code
... (somewhat more important) and protein factors (somewhat less important). Protein synthesis takes place in ribosomes — enormous complexes containing three large RNA molecules and more than 50 proteins. We will first take a look at the tRNA and the enzymes that ‘charge’ them with the correct amino aci ...
... (somewhat more important) and protein factors (somewhat less important). Protein synthesis takes place in ribosomes — enormous complexes containing three large RNA molecules and more than 50 proteins. We will first take a look at the tRNA and the enzymes that ‘charge’ them with the correct amino aci ...
Characterization of the binding properties of the Avian Coronavirus
... Protein biochemistry ................................................................................. 47 ...
... Protein biochemistry ................................................................................. 47 ...
Slayer of Lactobacillus helveticus ATCC 12046
... pH 7.2, and treated for 10 min in a boiling water bath; then the samples were centrifuged and 25 pl of the supernatant was mixed with 40 pl of the followingsolution: 0.1 % SDS,0.1 % 2-mercaptoethanol, 30% (w/v) glycerol, 0.2% Orange G in 0.01 M-Tris/HCl buffer, pH 7-2.Gels were stained with Coomassi ...
... pH 7.2, and treated for 10 min in a boiling water bath; then the samples were centrifuged and 25 pl of the supernatant was mixed with 40 pl of the followingsolution: 0.1 % SDS,0.1 % 2-mercaptoethanol, 30% (w/v) glycerol, 0.2% Orange G in 0.01 M-Tris/HCl buffer, pH 7-2.Gels were stained with Coomassi ...
Identification of a novel N-terminal hydrophobic sequence that
... (inset). To quantify the distribution of AAM-B, images of stained cells were systematically acquired until 50 cells had been photographed for each time point. The cells were then scored for the presence of either lipid droplet alone, or droplet plus reticular ER staining patterns. We did not detect ...
... (inset). To quantify the distribution of AAM-B, images of stained cells were systematically acquired until 50 cells had been photographed for each time point. The cells were then scored for the presence of either lipid droplet alone, or droplet plus reticular ER staining patterns. We did not detect ...
Lysines 72, 80 and 213 and aspartic acid 210 of the
... lysozyme as described (Maeda and Gasson, 1986) and boiled (5 min) in the presence of SDS-PAGE sample buffer. Subsequently, total cellular protein was separated on a 12.5% polyacrylamide-SDS gel and transferred to a nitrocellulose membrane (BA85; Schleicher & Schuell). The membrane was treated with r ...
... lysozyme as described (Maeda and Gasson, 1986) and boiled (5 min) in the presence of SDS-PAGE sample buffer. Subsequently, total cellular protein was separated on a 12.5% polyacrylamide-SDS gel and transferred to a nitrocellulose membrane (BA85; Schleicher & Schuell). The membrane was treated with r ...
Molecular Models for Biochemistry at CMU
... description. (All of the CMU cluster Macs and PCs have Chime installed): Chemscape Chime Plug-in v2.6. Get the Chime plug-in from the MDL Inc. software download page. It is now available for the Mac, Windows, and SGI IRIX computers (v0.9z, only). Internet Explorer 6, on a Windows platform, appears t ...
... description. (All of the CMU cluster Macs and PCs have Chime installed): Chemscape Chime Plug-in v2.6. Get the Chime plug-in from the MDL Inc. software download page. It is now available for the Mac, Windows, and SGI IRIX computers (v0.9z, only). Internet Explorer 6, on a Windows platform, appears t ...
Pseudomonas aeruginosa lytic bacteriophage oKMV
... scale yeast two-hybrid screen. To our knowledge, this study was the first time the yeast two-hybrid system was used in determining phage-host receptor interactions. This P. aeruginosa PAO1 protein was found to interact with phage phiKMV gp48. PA4736 is co-transcribed with the outer membrane protein ...
... scale yeast two-hybrid screen. To our knowledge, this study was the first time the yeast two-hybrid system was used in determining phage-host receptor interactions. This P. aeruginosa PAO1 protein was found to interact with phage phiKMV gp48. PA4736 is co-transcribed with the outer membrane protein ...
Protein tyrosine phosphorylation in plants: more
... whereas phosphorylation on Ser (90%) and Thr (10%) were predicted to be more abundant [33]. However, mediumand large-scale (several hundred to >10 000 sites per study) MS-based studies recently indicated that the frequency of Tyr phosphorylation ranges from 2% to 3% in animals [34–36] and <1% in yea ...
... whereas phosphorylation on Ser (90%) and Thr (10%) were predicted to be more abundant [33]. However, mediumand large-scale (several hundred to >10 000 sites per study) MS-based studies recently indicated that the frequency of Tyr phosphorylation ranges from 2% to 3% in animals [34–36] and <1% in yea ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.