How to move an amphipathic molecule across a lipid
... brucei incorporate radiolabelled oleoyl-CoA in an ABC transporter-dependent manner [40] and isolated human peroxisomes are able to oxidize C26:0 -CoA [19]. Stimulation of CTS-dependent ATPase activity by fatty acyl-CoAs together with their accumulation in Arabidopsis cts null mutants and the ability ...
... brucei incorporate radiolabelled oleoyl-CoA in an ABC transporter-dependent manner [40] and isolated human peroxisomes are able to oxidize C26:0 -CoA [19]. Stimulation of CTS-dependent ATPase activity by fatty acyl-CoAs together with their accumulation in Arabidopsis cts null mutants and the ability ...
ppt - eweb.furman.edu
... (suggesting gene duplication and subsequent evolution of new genes and elaboration of existing pathways) 5. Evolution of a genetic system - a reasonable hypothesis and significant corroborating evidence that it could happen. But no experimental evidence of the process evolving through all three step ...
... (suggesting gene duplication and subsequent evolution of new genes and elaboration of existing pathways) 5. Evolution of a genetic system - a reasonable hypothesis and significant corroborating evidence that it could happen. But no experimental evidence of the process evolving through all three step ...
Sia water test
... less than the values reported for normal serum macroglobuhns0 and the macroglobulin of patient C. K. (Table 2). The sialic acid content, however, was 6 times that of IgG and 20 per cent greater (1 per cent) than the sialic acid of the macroglobulin of patient C. K. (0.8 per cent), whose serum gave a ...
... less than the values reported for normal serum macroglobuhns0 and the macroglobulin of patient C. K. (Table 2). The sialic acid content, however, was 6 times that of IgG and 20 per cent greater (1 per cent) than the sialic acid of the macroglobulin of patient C. K. (0.8 per cent), whose serum gave a ...
file
... • Not consumed in reaction – single enzyme molecule can catalyze thousands or more reactions per second • enzymes unaffected by the reaction ...
... • Not consumed in reaction – single enzyme molecule can catalyze thousands or more reactions per second • enzymes unaffected by the reaction ...
P.abyssi PDF version
... (Makino et al., 2001; Zivanovic et al., 2002). Further comparison including the third sequenced Pyrococcus species, P. furiosus (Maeder et al., 1999) led to identification of DNA reorganization linked to IS-like elements and DNA integration within tRNA genes (Lecompte et al., 2001). These DNA rearra ...
... (Makino et al., 2001; Zivanovic et al., 2002). Further comparison including the third sequenced Pyrococcus species, P. furiosus (Maeder et al., 1999) led to identification of DNA reorganization linked to IS-like elements and DNA integration within tRNA genes (Lecompte et al., 2001). These DNA rearra ...
Factors Affecting Adsorption and Pre
... The effects of ionic strength on HSA and IgGadsorption was also studied by varying the NaCl concentration in 50 mM MES, pH 6.1. HSA was adsorbed to the three particles at 1 mg/mL added protein, 50 mM MES buffer, pH 6.1 and varying concentrations of sodium chloride. In Figure 9, the largest effect wa ...
... The effects of ionic strength on HSA and IgGadsorption was also studied by varying the NaCl concentration in 50 mM MES, pH 6.1. HSA was adsorbed to the three particles at 1 mg/mL added protein, 50 mM MES buffer, pH 6.1 and varying concentrations of sodium chloride. In Figure 9, the largest effect wa ...
Free fatty acids regulate the uncoupling protein and alternative
... such as linoleic acid (LA), to plant mitochondria results in mitochondrial uncoupling, while the presence of purine nucleotides, such as ATP or GTP which inhibit PUMP, and bovine serum album (BSA) which removes FFA, increases the membrane potential of these mitochondria [4,5,10]. Uncoupling by FFA, ...
... such as linoleic acid (LA), to plant mitochondria results in mitochondrial uncoupling, while the presence of purine nucleotides, such as ATP or GTP which inhibit PUMP, and bovine serum album (BSA) which removes FFA, increases the membrane potential of these mitochondria [4,5,10]. Uncoupling by FFA, ...
The Other Lives of Ribosomal Proteins - PDXScholar
... For the NMJ analysis, w+/-; ;;Mhc UAS-Gal4 larvae grown at 25°C were used. Drosophila larvae were carefully dissected using Jan’s Saline without CaCl 2. A longitudinal mid-dorsal incision was made and the edges of the tissues were pinned so that the preparation was spread out on a glass slide in the ...
... For the NMJ analysis, w+/-; ;;Mhc UAS-Gal4 larvae grown at 25°C were used. Drosophila larvae were carefully dissected using Jan’s Saline without CaCl 2. A longitudinal mid-dorsal incision was made and the edges of the tissues were pinned so that the preparation was spread out on a glass slide in the ...
Meal ingestion, amino acids and brain
... 10 d before experimentation. During this time, water and food (a 17% casein diet; see below) were provided ad libitum. The animals were exposed to 12 h of light daily (2200 h–1000 h; i.e., a reversed lighting cycle) and an ambient temperature of 22 °C. All experiments were approved by the University ...
... 10 d before experimentation. During this time, water and food (a 17% casein diet; see below) were provided ad libitum. The animals were exposed to 12 h of light daily (2200 h–1000 h; i.e., a reversed lighting cycle) and an ambient temperature of 22 °C. All experiments were approved by the University ...
Complete DNA sequence of yeast chromosome II.
... eukaryotes in the yeast system. Two years ago a consortium of 35 European laboratorics published the first complete sequence of a eukaryotic chromosome: chromosome III of S.cerevisiae {Oliver el al., 1992). For the past 3 years our consortium has tumed its efforts to the sequencing of yeast chromoso ...
... eukaryotes in the yeast system. Two years ago a consortium of 35 European laboratorics published the first complete sequence of a eukaryotic chromosome: chromosome III of S.cerevisiae {Oliver el al., 1992). For the past 3 years our consortium has tumed its efforts to the sequencing of yeast chromoso ...
Isotope-labeled Peptides
... API products Bachem offers deuterated, 13C-, and 15Nlabeled analogs for pharmacokinetic/pharmacodynamic studies as well as analogs suitable for radiolabeling. Contrary to working with radionuclide-labeled peptides, licenses and additional safety measures are not required for handling stable isotope- ...
... API products Bachem offers deuterated, 13C-, and 15Nlabeled analogs for pharmacokinetic/pharmacodynamic studies as well as analogs suitable for radiolabeling. Contrary to working with radionuclide-labeled peptides, licenses and additional safety measures are not required for handling stable isotope- ...
Chem 465 Biochemistry II
... Pyruvate dehydrogenase and á-ketoglutarate dehydrogenase are very similar in structure and mechanism. Both are large enzyme complexes that contain multiple copies of three major proteins usually called simply E1, E2, and E3. Both enzyme complexes also utilize the cofactors thiamine pyrophosphate, li ...
... Pyruvate dehydrogenase and á-ketoglutarate dehydrogenase are very similar in structure and mechanism. Both are large enzyme complexes that contain multiple copies of three major proteins usually called simply E1, E2, and E3. Both enzyme complexes also utilize the cofactors thiamine pyrophosphate, li ...
novel nucleotide carrier proteins of Protochlamydia
... phylogenetic trees containing all known NTT proteins PamNTT2, PamNTT3 and PamNTT5 formed deep branches between the ATP/ADP translocases from rickettsiae, chlamydiae, plant plastids and other intracellular bacteria, and the proton-driven nucleotide importers of chlamydiae (Fig. S2). They thus could n ...
... phylogenetic trees containing all known NTT proteins PamNTT2, PamNTT3 and PamNTT5 formed deep branches between the ATP/ADP translocases from rickettsiae, chlamydiae, plant plastids and other intracellular bacteria, and the proton-driven nucleotide importers of chlamydiae (Fig. S2). They thus could n ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... were added and the total volume was made to 2.9ml with water. The reaction was started by adding 0.1ml of mitochondrial suspension. The enzyme activity was measured at 420 nm in a Shimadzu- UV-1601 spectrophotometer using UV PC Software package. The activity of malate dehydrogenase was expressed as ...
... were added and the total volume was made to 2.9ml with water. The reaction was started by adding 0.1ml of mitochondrial suspension. The enzyme activity was measured at 420 nm in a Shimadzu- UV-1601 spectrophotometer using UV PC Software package. The activity of malate dehydrogenase was expressed as ...
Crude protein and amino acids content in some common
... Sartorius stedim filter paper (Biotech, 1288) into 100 mL flask. The samples were then diluted with deionized water and then filtered with aqueous syringe nylon filter 0.2 µm. A total of 2 mL filtered sample were cooled at -20°C prior to amino acid analysis using HPLC standart procedure. Data analys ...
... Sartorius stedim filter paper (Biotech, 1288) into 100 mL flask. The samples were then diluted with deionized water and then filtered with aqueous syringe nylon filter 0.2 µm. A total of 2 mL filtered sample were cooled at -20°C prior to amino acid analysis using HPLC standart procedure. Data analys ...
Crystal structure of yeast hexokinase Pl in complex
... sets of data extended to include all data from 12.9 to 2.95 Å. Thirteen thousand six hundred forty-two reflections were used for the refinement, while 715 reflections, randomly selected and set aside during refinement process, were used for R-free calculation. Rounds of positional and group B-facto ...
... sets of data extended to include all data from 12.9 to 2.95 Å. Thirteen thousand six hundred forty-two reflections were used for the refinement, while 715 reflections, randomly selected and set aside during refinement process, were used for R-free calculation. Rounds of positional and group B-facto ...
Angiotensin I-converting enzyme-inhibitory peptide fractions
... grain and defatted flour used in this study. The present results show that crude protein (N 5.85) was not appreciably concentrated when the flour was defatted with acetone. On the contrary, ash and carbohydrates increased because of this process. Even though this protein concentration is not very si ...
... grain and defatted flour used in this study. The present results show that crude protein (N 5.85) was not appreciably concentrated when the flour was defatted with acetone. On the contrary, ash and carbohydrates increased because of this process. Even though this protein concentration is not very si ...
Mass Spectrometry of Proteinstutorial
... The terms in parentheses (related to a fixed distance and accelerating potential) remain constant thus m/z can be determined from t2. As all the ions are accelerated with the same potential then they all have the same kinetic energy. Singly charged ions with a larger mass will travel with a lower ve ...
... The terms in parentheses (related to a fixed distance and accelerating potential) remain constant thus m/z can be determined from t2. As all the ions are accelerated with the same potential then they all have the same kinetic energy. Singly charged ions with a larger mass will travel with a lower ve ...
Word - Food Standards Australia New Zealand
... Cotton (Gossypium hirsutum L.) is grown as a commercial crop worldwide and has a long history of safe use for both human food and stock feed. Cotton is grown typically in arid regions of the tropics and sub-tropics. It is primarily grown as a fibre crop with the resulting cottonseed being processed ...
... Cotton (Gossypium hirsutum L.) is grown as a commercial crop worldwide and has a long history of safe use for both human food and stock feed. Cotton is grown typically in arid regions of the tropics and sub-tropics. It is primarily grown as a fibre crop with the resulting cottonseed being processed ...
food derived from insect-protected, glufosinate ammonium
... Cotton (Gossypium hirsutum L.) is grown as a commercial crop worldwide and has a long history of safe use for both human food and stock feed. Cotton is grown typically in arid regions of the tropics and sub-tropics. It is primarily grown as a fibre crop with the resulting cottonseed being processed ...
... Cotton (Gossypium hirsutum L.) is grown as a commercial crop worldwide and has a long history of safe use for both human food and stock feed. Cotton is grown typically in arid regions of the tropics and sub-tropics. It is primarily grown as a fibre crop with the resulting cottonseed being processed ...
PowerPoint 演示文稿
... • (1) Bacteriophage and viral transcipts and (2) many mRNAs for hormones in eukaryotes (e.g. pro-opiomelanocortin) are translated to give a single polypeptide chain that is cleaved subsequently by specific proteases to produce multiple mature protein from one translation product. The parent polypept ...
... • (1) Bacteriophage and viral transcipts and (2) many mRNAs for hormones in eukaryotes (e.g. pro-opiomelanocortin) are translated to give a single polypeptide chain that is cleaved subsequently by specific proteases to produce multiple mature protein from one translation product. The parent polypept ...
Microreviews in Cell and Molecular Biology
... disease in the United States, it can lead to more severe complications than implied by the trademark abnormal fat deposits. Cushing syndrome can contribute to heightened blood pressure, diabetes, depression, bone loss, and, as stated previously, cancer (2). However, treatment options are somewhat li ...
... disease in the United States, it can lead to more severe complications than implied by the trademark abnormal fat deposits. Cushing syndrome can contribute to heightened blood pressure, diabetes, depression, bone loss, and, as stated previously, cancer (2). However, treatment options are somewhat li ...
Position versus Substrate
... The most obvious questions to be asked about any transferase are two very simple ones: What are their substrates? How specific are the individual enzymes? Earlier reports were often forced to work with only partly purified proteins or crude cell extracts, which were not suited to unequivocally corre ...
... The most obvious questions to be asked about any transferase are two very simple ones: What are their substrates? How specific are the individual enzymes? Earlier reports were often forced to work with only partly purified proteins or crude cell extracts, which were not suited to unequivocally corre ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.