Pseudomonas aeruginosa lytic bacteriophage oKMV
... scale yeast two-hybrid screen. To our knowledge, this study was the first time the yeast two-hybrid system was used in determining phage-host receptor interactions. This P. aeruginosa PAO1 protein was found to interact with phage phiKMV gp48. PA4736 is co-transcribed with the outer membrane protein ...
... scale yeast two-hybrid screen. To our knowledge, this study was the first time the yeast two-hybrid system was used in determining phage-host receptor interactions. This P. aeruginosa PAO1 protein was found to interact with phage phiKMV gp48. PA4736 is co-transcribed with the outer membrane protein ...
blast
... • The sequence accession name: this takes you to the database entry that contains the sequence. • Description of the match organism and any assigned/putative function • The alignment score. Higher scoring hits are at the top • Query coverage is how much of your sequence aligned to the match • The ex ...
... • The sequence accession name: this takes you to the database entry that contains the sequence. • Description of the match organism and any assigned/putative function • The alignment score. Higher scoring hits are at the top • Query coverage is how much of your sequence aligned to the match • The ex ...
Lab 5: Proteins and the small molecules that love them
... catalyze. In the case of HIV Protease, this is abundantly clear as Protease helps coordinate a water molecule to cleave the polyprotein backbone. Two of the key residues for this are Asp 25 and Asp 25’. The prime is used to notate that the residues are on separate, but identical subunits. Asp 25 and ...
... catalyze. In the case of HIV Protease, this is abundantly clear as Protease helps coordinate a water molecule to cleave the polyprotein backbone. Two of the key residues for this are Asp 25 and Asp 25’. The prime is used to notate that the residues are on separate, but identical subunits. Asp 25 and ...
07_Metabolism of aminoacids
... Maple syrup urine disease - the disorder of the oxidative decarboxylation of -ketoacids derived from valine, isoleucine, and leucine caused by the missing or defect of branched-chain dehydrogenase. The levels of branched-chain amino acids and corresponding -ketoacids are markedly elevated in both ...
... Maple syrup urine disease - the disorder of the oxidative decarboxylation of -ketoacids derived from valine, isoleucine, and leucine caused by the missing or defect of branched-chain dehydrogenase. The levels of branched-chain amino acids and corresponding -ketoacids are markedly elevated in both ...
Knocking Down of Isoprene Emission Modiies the
... biological membranes. The fluidity of the thylakoid membrane is essential for photosynthetic processes. The thylakoid membranes are highly organized internal membrane chloroplast systems that conduct the light reactions of photosynthesis. These membranes comprise pigments and proteins organized in co ...
... biological membranes. The fluidity of the thylakoid membrane is essential for photosynthetic processes. The thylakoid membranes are highly organized internal membrane chloroplast systems that conduct the light reactions of photosynthesis. These membranes comprise pigments and proteins organized in co ...
The Invention of Proteomic Code and mRNA
... as the chief proponent of the Big Bang theory in cosmology. He proposed a Diamond Code [4-6] where DNA acted directly as a template for assembling amino acids into proteins. Various combinations of bases along one of the grooves in the double helix could form distinctively shaped cavities into which ...
... as the chief proponent of the Big Bang theory in cosmology. He proposed a Diamond Code [4-6] where DNA acted directly as a template for assembling amino acids into proteins. Various combinations of bases along one of the grooves in the double helix could form distinctively shaped cavities into which ...
Sequencing the World of Possibilities for Energy & Environment
... a curated protein sequence database high level of annotation (such as the description of the function of a protein, its domains structure, post-translational modifications, variants, etc.) ...
... a curated protein sequence database high level of annotation (such as the description of the function of a protein, its domains structure, post-translational modifications, variants, etc.) ...
Enzyme Hydrolyzed Collagen Protein
... the human body and in the same sizes and amounts the human body utilizes (making it the most bioidentical). The low-temperature, gentle drying cycle ensures that biologically active state of all cofactors and amino acids peptides remain intact. Thus the end product will have a higher concentration o ...
... the human body and in the same sizes and amounts the human body utilizes (making it the most bioidentical). The low-temperature, gentle drying cycle ensures that biologically active state of all cofactors and amino acids peptides remain intact. Thus the end product will have a higher concentration o ...
Chloroplast phosphoglycerate kinase from Euglena gracilis
... chloroplasts. Chloroplasts isolated as described previously [26] were suspended in buffer 2 and lysed by sonication for 2 s. The lysate was centrifuged for 20 min at 30 000 g, and the supernatant was diluted with buffer 2 to a final volume of 20 mL and applied to a 1.6 · 5 cm Reactive blue 72 column. ...
... chloroplasts. Chloroplasts isolated as described previously [26] were suspended in buffer 2 and lysed by sonication for 2 s. The lysate was centrifuged for 20 min at 30 000 g, and the supernatant was diluted with buffer 2 to a final volume of 20 mL and applied to a 1.6 · 5 cm Reactive blue 72 column. ...
Applied Environmnetal Microbiology
... a cryI-specific probe that is highly homologous to all known cryI genes in order to compare the relative distribution of cryV and cryI genes among B. thuringiensis strains. Of the 24 strains, 14 showed a positive signal to the cryI-specific probe, indicating that these strains contain cryI-type gene ...
... a cryI-specific probe that is highly homologous to all known cryI genes in order to compare the relative distribution of cryV and cryI genes among B. thuringiensis strains. Of the 24 strains, 14 showed a positive signal to the cryI-specific probe, indicating that these strains contain cryI-type gene ...
Studies on the Reactivity towards Pyridoxal 5`
... sequence of the bovine enzyme (Wootton et al., 1973). These include a 30-residue fragment that aligns with residues 105-134 of the bovine enzyme sequence described by Moon et al. (1972), including a lysine residue corresponding to lysine-126, which in the bovine enzyme is highly reactive towards pyr ...
... sequence of the bovine enzyme (Wootton et al., 1973). These include a 30-residue fragment that aligns with residues 105-134 of the bovine enzyme sequence described by Moon et al. (1972), including a lysine residue corresponding to lysine-126, which in the bovine enzyme is highly reactive towards pyr ...
Young Innovators 2009
... Purpose: The highly polymorphic organic transport protein, OCT1; SLC22A1 is involved in the translocation of the hydrophilic anti-diabetic drug metformin from the blood into hepatocytes. Since many drugs are inhibitors of the OCT1 [1], this may lead to drug-drug interactions at the OCT1. The risk of ...
... Purpose: The highly polymorphic organic transport protein, OCT1; SLC22A1 is involved in the translocation of the hydrophilic anti-diabetic drug metformin from the blood into hepatocytes. Since many drugs are inhibitors of the OCT1 [1], this may lead to drug-drug interactions at the OCT1. The risk of ...
A bioinformatics study concerning the structural and functional
... The structural and functional characteristics of caveolin proteins have not yet been well defined, but some useful information can be obtained through sequence based predictions using structural bioinformatics tools. In the present study, a bioinformatics investigation was performed in which, to the ...
... The structural and functional characteristics of caveolin proteins have not yet been well defined, but some useful information can be obtained through sequence based predictions using structural bioinformatics tools. In the present study, a bioinformatics investigation was performed in which, to the ...
Characterization of a P-lactamase produced by
... the p-lactamase was performed in two stages. Crude sonicate was loaded onto a 1.6 x 80 cm column of Ultrogel AcA44 (LKB) and run in 50 mM-potassium phosphate pH 7.5, 10% (v/v) glycerol, 1 mM-EDTA and 5 mM-2-mercaptoethanol (flow rate 8 ml h-I). Fractions of 2 ml were collected and the presence of p- ...
... the p-lactamase was performed in two stages. Crude sonicate was loaded onto a 1.6 x 80 cm column of Ultrogel AcA44 (LKB) and run in 50 mM-potassium phosphate pH 7.5, 10% (v/v) glycerol, 1 mM-EDTA and 5 mM-2-mercaptoethanol (flow rate 8 ml h-I). Fractions of 2 ml were collected and the presence of p- ...
Analysis of the 3′-terminal nucleotide sequence of vesicular
... transcription, 10 x the concentration of the corresponding dNTP; (b) for 10 - 100 nucleotides, 1 x the dNTP concentration; (c) for more than 100 nucleotides, 0.5 x or 0,25 x. the dNTP concentration. The products of the reactions were fractionated by electrophoresis in polyacrylamide slab gels contai ...
... transcription, 10 x the concentration of the corresponding dNTP; (b) for 10 - 100 nucleotides, 1 x the dNTP concentration; (c) for more than 100 nucleotides, 0.5 x or 0,25 x. the dNTP concentration. The products of the reactions were fractionated by electrophoresis in polyacrylamide slab gels contai ...
Enzymes
... • Enzymes are globular proteins which act as catalysts of chemical reactions • Without enzymes to catalyze them, many chemical processes happen at a very slow rate in living organisms • By making some enzymes and not others, cells can control what chemical reactions happen in their cytoplasm ...
... • Enzymes are globular proteins which act as catalysts of chemical reactions • Without enzymes to catalyze them, many chemical processes happen at a very slow rate in living organisms • By making some enzymes and not others, cells can control what chemical reactions happen in their cytoplasm ...
soy protein concentrate - US Soybean Export Council
... selected and cleaned as the first step in soy product processing. The clean soybeans are dehulled, then the oil is extracted for cooking, biodiesel and other uses. The residue, consisting of defatted white flakes, can then be ground to make soy flour, toasted and ground to make soybean meal, or subj ...
... selected and cleaned as the first step in soy product processing. The clean soybeans are dehulled, then the oil is extracted for cooking, biodiesel and other uses. The residue, consisting of defatted white flakes, can then be ground to make soy flour, toasted and ground to make soybean meal, or subj ...
Characterisation of Milk Proteins in Confectionery Products
... The proteins in milk, although representing only a small percentage (3-4 %) of its total composition are considered to possess unique structural and functional properties which will influence these final quality parameters as well as affecting processing. Of particular impor tance in confectionery a ...
... The proteins in milk, although representing only a small percentage (3-4 %) of its total composition are considered to possess unique structural and functional properties which will influence these final quality parameters as well as affecting processing. Of particular impor tance in confectionery a ...
Structure-Function Analysis of the UDP-N-acetyl-D
... Expression and Immunopurification of Recombinant Proteins—Recombinant ppGaNTases were expressed as secreted products from COS7 cells and partially purified from cell culture medium as described previously (4). To normalize the quantity of recombinant proteins employed for enzyme assays, aliquots of ...
... Expression and Immunopurification of Recombinant Proteins—Recombinant ppGaNTases were expressed as secreted products from COS7 cells and partially purified from cell culture medium as described previously (4). To normalize the quantity of recombinant proteins employed for enzyme assays, aliquots of ...
A Survey of Left-handed Helices in Protein Structures
... very rare. Stretches of amino acids with unusual backbone conformations (e.g. left-handed helices) often appear at ligand-binding sites, protein–protein interfaces or other functional sites. It has been suggested that proteins may sacrifice a part of their stability to form an effective functional s ...
... very rare. Stretches of amino acids with unusual backbone conformations (e.g. left-handed helices) often appear at ligand-binding sites, protein–protein interfaces or other functional sites. It has been suggested that proteins may sacrifice a part of their stability to form an effective functional s ...
Conserved Positions for Ribose Recognition: Importance of Water
... are involved in energy exchange processes in biological systems, whereas FAD, among other dinucleotides, acts as an ancillary electron carrier for a wide variety of enzymes involved in metabolism. Knowledge of the principles that govern ribose recognition by proteins is sketchy because tertiary and ...
... are involved in energy exchange processes in biological systems, whereas FAD, among other dinucleotides, acts as an ancillary electron carrier for a wide variety of enzymes involved in metabolism. Knowledge of the principles that govern ribose recognition by proteins is sketchy because tertiary and ...
Protein kinase inhibition: natural and synthetic variations on a theme
... Not only are there thousands of proteins that utilize this regulatory mechanism, but the number of protein kinases that catalyze these phosphoryl transfer reactions also number in the hundreds (reviewed in [Z]). It is predicted that the human genome alone encodes over 2000 protein kinases. Since the ...
... Not only are there thousands of proteins that utilize this regulatory mechanism, but the number of protein kinases that catalyze these phosphoryl transfer reactions also number in the hundreds (reviewed in [Z]). It is predicted that the human genome alone encodes over 2000 protein kinases. Since the ...
Phosphate Groups Modifying Myelin Basic Proteins Are
... protein was subjected to discontinuous PAGE in buffers containing SDS (30) as described previously (25, 45). The procedure was modified by increasing the bisacrylamide concentration in the 15% acrylamide running gel solution so that the ratio of acrylamide/bisacrylamide was 20: I; this modification ...
... protein was subjected to discontinuous PAGE in buffers containing SDS (30) as described previously (25, 45). The procedure was modified by increasing the bisacrylamide concentration in the 15% acrylamide running gel solution so that the ratio of acrylamide/bisacrylamide was 20: I; this modification ...
Synaptic adhesion molecules - UCSF Physiology
... some integrins. Intracellular partners include the protein tyrosine phosphatase RPTPα, the tyrosine kinase p59Fyn and spectrin. The most remarkable feature of NCAM is that it is virtually the sole acceptor for long linear homopolymers of α2, 8-linked sialic acid residues (polysialic acid, PSA). The ...
... some integrins. Intracellular partners include the protein tyrosine phosphatase RPTPα, the tyrosine kinase p59Fyn and spectrin. The most remarkable feature of NCAM is that it is virtually the sole acceptor for long linear homopolymers of α2, 8-linked sialic acid residues (polysialic acid, PSA). The ...
PPT - Portland State University
... The signal output from the pH meter was acquired by a computer through a 12-bit A/D board operated at 1kHz rate. Samples that contained 5 M rhodopsin , Gt and PDE, 50 M GTP and 1 mM cGMP in TBS buffer (pH 8.0) were preincubated at 37 C in a thermo-regulated micro-cuvette in the dark for 10 minute ...
... The signal output from the pH meter was acquired by a computer through a 12-bit A/D board operated at 1kHz rate. Samples that contained 5 M rhodopsin , Gt and PDE, 50 M GTP and 1 mM cGMP in TBS buffer (pH 8.0) were preincubated at 37 C in a thermo-regulated micro-cuvette in the dark for 10 minute ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.