Partial Purification and Characterization of Three Flavonol
... was carried out on Avicel cellulose using H20 or n-BuOHHOAc-H20 (3:1:1 or 4:1:5, v/v/v) as solvents. Paper electrophoresis was carried out as previously described (12). Developed chromatograms were visualized in UV light (366 nm) before and after spraying with 1% diphenylborinate and then autoradiog ...
... was carried out on Avicel cellulose using H20 or n-BuOHHOAc-H20 (3:1:1 or 4:1:5, v/v/v) as solvents. Paper electrophoresis was carried out as previously described (12). Developed chromatograms were visualized in UV light (366 nm) before and after spraying with 1% diphenylborinate and then autoradiog ...
Postexercise nutrient intake timing in humans is critical to recovery
... containing 1% t-butyldimethylchlorosilane (MtBSTFA ⫹ 1% tBDMCS; Regis Technologies, Morton Grove, IL). The derivatized samples were then analyzed with GC-MS (HewlettPackard 5890a GC and 5970 MS, San Fernando, CA) for plasma leucine and KIC enrichments. For determination of [2H5]glycerol enrichment, ...
... containing 1% t-butyldimethylchlorosilane (MtBSTFA ⫹ 1% tBDMCS; Regis Technologies, Morton Grove, IL). The derivatized samples were then analyzed with GC-MS (HewlettPackard 5890a GC and 5970 MS, San Fernando, CA) for plasma leucine and KIC enrichments. For determination of [2H5]glycerol enrichment, ...
國立清華大學 - 罕見疾病基金會
... amino acid residues when samples were separated by blue native PAGE (BN-PAGE) or SDS-PAGE. Similarly, fully assembled mitochondrial complex I was decreased in the BN-PAGE analysis with anti-GRIM19, NDUFA9, or NDUFS3 antibodies, but the assembly of complex II, III and IV was not affected [12]. In sum ...
... amino acid residues when samples were separated by blue native PAGE (BN-PAGE) or SDS-PAGE. Similarly, fully assembled mitochondrial complex I was decreased in the BN-PAGE analysis with anti-GRIM19, NDUFA9, or NDUFS3 antibodies, but the assembly of complex II, III and IV was not affected [12]. In sum ...
High-resolution mapping of protein sequence
... encodes 25 variable residues that span the structured region of the WW domain, including both of the conserved tryptophan residues, and encompass the binding interface (Fig. 1a). We acquired sequencing data for the input variant library as well as after three and six rounds of selection, with an ave ...
... encodes 25 variable residues that span the structured region of the WW domain, including both of the conserved tryptophan residues, and encompass the binding interface (Fig. 1a). We acquired sequencing data for the input variant library as well as after three and six rounds of selection, with an ave ...
PhoR, PhoP and MshC: Three essential proteins of Mycobacterium
... attempted to crystallize the full-length M. smegmatis MshC using Km values of ATP, Lcysteine, and the potential inhibitor glucosazide inositol. Thus far, crystallization attempts have not been successful. We also attempted to develop a high-throughput assay for drug screening. Using the quinaldine r ...
... attempted to crystallize the full-length M. smegmatis MshC using Km values of ATP, Lcysteine, and the potential inhibitor glucosazide inositol. Thus far, crystallization attempts have not been successful. We also attempted to develop a high-throughput assay for drug screening. Using the quinaldine r ...
power-point slides
... The problem is analogous to the problems of representing the surface of the earth on a two-dimensional map Reduction of the dimensions leads to distortions of scales, ...
... The problem is analogous to the problems of representing the surface of the earth on a two-dimensional map Reduction of the dimensions leads to distortions of scales, ...
Supplemental Materials
... If there is any difference in the colored beads between the normal and mutated protein, write the difference here ____________________________________________________________. 5) Now we need to fold this amino acid sequence into a 3D shape for the hemoglobin protein to function properly. How do you ...
... If there is any difference in the colored beads between the normal and mutated protein, write the difference here ____________________________________________________________. 5) Now we need to fold this amino acid sequence into a 3D shape for the hemoglobin protein to function properly. How do you ...
Gene Section LCK (lymphocyte-specific protein tyrosine kinase) Atlas of Genetics and Cytogenetics
... unique amino-terminal regions, followed by Src homology domains SH3 and SH2, a tyrosine kinase catalytic domain, and C-terminal regulatory domain. Lck associates with the inner face of the plasma membrane through its amino-terminus. This interaction is mediated by both myristic acid and palmitic aci ...
... unique amino-terminal regions, followed by Src homology domains SH3 and SH2, a tyrosine kinase catalytic domain, and C-terminal regulatory domain. Lck associates with the inner face of the plasma membrane through its amino-terminus. This interaction is mediated by both myristic acid and palmitic aci ...
MORE ABOUT SOLUTIONS - Bio-Link
... Once gloves have come in contact with a surface that was touched by skin (for example, a pen, notebook, laboratory bench, etc.) the gloves should be changed ...
... Once gloves have come in contact with a surface that was touched by skin (for example, a pen, notebook, laboratory bench, etc.) the gloves should be changed ...
Properties of Enzymes
... (c) Enzymes are charged molecules: Due to the presences of amino acids, each enzyme has a charge. The charge depends on the pH of the solution. At very low pH the amino acids are fully protonated and there is a positive charge on the proteins; as pH is increased, the protein losses a proton to neutr ...
... (c) Enzymes are charged molecules: Due to the presences of amino acids, each enzyme has a charge. The charge depends on the pH of the solution. At very low pH the amino acids are fully protonated and there is a positive charge on the proteins; as pH is increased, the protein losses a proton to neutr ...
Identification of Two Mammalian Reductases
... Herndon, VA), 1 mM sodium pyruvate, 100 units/ml penicillin G sodium, and 100 g/ml streptomycin sulfate. HepG2 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum, 100 units/ml penicillin G sodium, and 100 g/ml streptomycin sulfate, respectively. dsRNAs ...
... Herndon, VA), 1 mM sodium pyruvate, 100 units/ml penicillin G sodium, and 100 g/ml streptomycin sulfate. HepG2 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum, 100 units/ml penicillin G sodium, and 100 g/ml streptomycin sulfate, respectively. dsRNAs ...
enzymes lecture 1
... (C) Apoenzyme (D) Holoenzyme 3- Enzymes are largely ……………… in their chemical nature. (A) Lipids (B) Steroids (C) Protein (D) All A, B and C ...
... (C) Apoenzyme (D) Holoenzyme 3- Enzymes are largely ……………… in their chemical nature. (A) Lipids (B) Steroids (C) Protein (D) All A, B and C ...
SOMAmer® anti-Carbonic anhydrase 3 Reagent
... No binding was observed with Carbonic anhydrase I, Carbonic anhydrase II, Carbonic anhydrase XIII, Carbonic anhydrase VII, Carbonic anhydrase 6, Carbonic anhydrase X, or Carbonic anhydrase 9. Affinity The Kd for Carbonic anhydrase 3 binding to the SOMAmer reagent, measured ...
... No binding was observed with Carbonic anhydrase I, Carbonic anhydrase II, Carbonic anhydrase XIII, Carbonic anhydrase VII, Carbonic anhydrase 6, Carbonic anhydrase X, or Carbonic anhydrase 9. Affinity The Kd for Carbonic anhydrase 3 binding to the SOMAmer reagent, measured ...
Enzymes of the mevalonate pathway of isoprenoid
... reaction intermediate. The purified avian liver enzyme was used to identify cysteine in formation of acetyl-S-enzyme and enzymeS-HMG-CoA covalent reaction intermediates [23,24] that are depicted in Scheme 3, which outlines the chemistry of the reaction. A mitochondrial isoform [25] supports the ketog ...
... reaction intermediate. The purified avian liver enzyme was used to identify cysteine in formation of acetyl-S-enzyme and enzymeS-HMG-CoA covalent reaction intermediates [23,24] that are depicted in Scheme 3, which outlines the chemistry of the reaction. A mitochondrial isoform [25] supports the ketog ...
Distinct Lytic Vacuolar Compartments are Embedded
... TIP1;1::GFP under the control of TIP1;1 native promoter Based on the observation of TIP1;1::GFP fusion protein fluorescence, Hunter and co-workers reported that TIP1;1 is not present in Arabidopsis seedlings during early seed germination and appears only 3.5 d after germination when it replaces a-TI ...
... TIP1;1::GFP under the control of TIP1;1 native promoter Based on the observation of TIP1;1::GFP fusion protein fluorescence, Hunter and co-workers reported that TIP1;1 is not present in Arabidopsis seedlings during early seed germination and appears only 3.5 d after germination when it replaces a-TI ...
Supplementary Information
... resuspended in phosphate buffered saline (PBS) and then captured onto MSD 96well high bind plates at 30,000 cells per well by incubating at 37⁰C for 1.5 hours. The plates were blocked with 15% fetal bovine serum (FBS) (or heat-inactivated FBS) for 30 min at room temperature with gentle agitation. Th ...
... resuspended in phosphate buffered saline (PBS) and then captured onto MSD 96well high bind plates at 30,000 cells per well by incubating at 37⁰C for 1.5 hours. The plates were blocked with 15% fetal bovine serum (FBS) (or heat-inactivated FBS) for 30 min at room temperature with gentle agitation. Th ...
Pupko_pairwise
... B. Take one sequence randomly shuffle it -> find score with the second sequence. Repeat 100,000 times. If the score in A is at the top 5% of the scores in B the similarity is significant. ...
... B. Take one sequence randomly shuffle it -> find score with the second sequence. Repeat 100,000 times. If the score in A is at the top 5% of the scores in B the similarity is significant. ...
BS 11 First Mid-Term Answer Key Spring 1998
... before, but they may have been changed. READ the questions carefully! (2 pt) A) An H-bond between two H2O molecules in isolation is about 10 times stronger energetically (∆H) than a van der Waals contact between two xenon atoms. ...
... before, but they may have been changed. READ the questions carefully! (2 pt) A) An H-bond between two H2O molecules in isolation is about 10 times stronger energetically (∆H) than a van der Waals contact between two xenon atoms. ...
MIAPE_Quant_v1.0_Template
... 5.1 Quantification values at peptide and/or feature level: Actual quantification values achieved for each peptide and/or, in case of feature-based quantification, for the corresponding features (mapped back from each peptide), together with their estimated confidence. Report each quantification valu ...
... 5.1 Quantification values at peptide and/or feature level: Actual quantification values achieved for each peptide and/or, in case of feature-based quantification, for the corresponding features (mapped back from each peptide), together with their estimated confidence. Report each quantification valu ...
Higher plant cellulose synthases | Genome Biology | Full Text
... protein is an amino acid domain that bears some resemblance to a zinc finger or LIM transcription factor. It is thought that this domain might play a role in protein-protein interactions in the CesA complex. Within this domain is a strictly conserved sequence motif, the CxxC motif, beginning 10-40 ...
... protein is an amino acid domain that bears some resemblance to a zinc finger or LIM transcription factor. It is thought that this domain might play a role in protein-protein interactions in the CesA complex. Within this domain is a strictly conserved sequence motif, the CxxC motif, beginning 10-40 ...
PROTEIN SYNTHESIS
... Eukaryotes have only one release factor eRF which requires GTP termination of protein synthesis. It recognizes all the three stop codons. In eukaryotes the mRNA is synthesized in the nucleus, then processed, modified and passed on into the cytoplasm through nucleopores. The protein synthesis takes p ...
... Eukaryotes have only one release factor eRF which requires GTP termination of protein synthesis. It recognizes all the three stop codons. In eukaryotes the mRNA is synthesized in the nucleus, then processed, modified and passed on into the cytoplasm through nucleopores. The protein synthesis takes p ...
Tonoplast Intrinsic Protein Isoforms as Markers for Vacuolar Functions
... quences (Jauh et al., 1998). The specificity of MAb351 was also examined by using protein gel blot analysis to compare anti–a-TIP and anti–d-TIP peptide antibodies (Figure 1B). The anti–a-TIP antibodies identified a major band of the appropriate 26-kD size, but only in the pea root tip (Figure 1B, l ...
... quences (Jauh et al., 1998). The specificity of MAb351 was also examined by using protein gel blot analysis to compare anti–a-TIP and anti–d-TIP peptide antibodies (Figure 1B). The anti–a-TIP antibodies identified a major band of the appropriate 26-kD size, but only in the pea root tip (Figure 1B, l ...
as a PDF
... quences (Jauh et al., 1998). The specificity of MAb351 was also examined by using protein gel blot analysis to compare anti–a-TIP and anti–d-TIP peptide antibodies (Figure 1B). The anti–a-TIP antibodies identified a major band of the appropriate 26-kD size, but only in the pea root tip (Figure 1B, l ...
... quences (Jauh et al., 1998). The specificity of MAb351 was also examined by using protein gel blot analysis to compare anti–a-TIP and anti–d-TIP peptide antibodies (Figure 1B). The anti–a-TIP antibodies identified a major band of the appropriate 26-kD size, but only in the pea root tip (Figure 1B, l ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.