TAR-RNA binding by HIV-1 Tat protein is
... peptide (12 residues) corresponding to the basic region of HIV-1 Tat protein with a Kd of 26 µM. In contrast, our competition binding experiments demonstrate that the affinity of the mirrorimage (L-enantiomer) of wild-type TAR for the Tat protein is 1000-fold better, thereby matching the affinity of ...
... peptide (12 residues) corresponding to the basic region of HIV-1 Tat protein with a Kd of 26 µM. In contrast, our competition binding experiments demonstrate that the affinity of the mirrorimage (L-enantiomer) of wild-type TAR for the Tat protein is 1000-fold better, thereby matching the affinity of ...
Poster
... electrical charges: positively charged nuclei and a negatively charged electron cloud. Every electrical charge creates an electric field that can exert a force on other charges. All the charges on a protein combine to form a electric field around and within myoglobin. This electrostatic field determ ...
... electrical charges: positively charged nuclei and a negatively charged electron cloud. Every electrical charge creates an electric field that can exert a force on other charges. All the charges on a protein combine to form a electric field around and within myoglobin. This electrostatic field determ ...
Poster
... other parts of the body are injured, new cells are generated to replace the damaged cells. On the other hand, the spinal cord is strikingly different because nerve cells don’t regenerate, making the process of rehabilitating injured spines complex and nearly impossible. This is even more drastic whe ...
... other parts of the body are injured, new cells are generated to replace the damaged cells. On the other hand, the spinal cord is strikingly different because nerve cells don’t regenerate, making the process of rehabilitating injured spines complex and nearly impossible. This is even more drastic whe ...
Application Note
... development of a reliable, rapid and accurate method of analysis for assessing the quality of foods for regulatory purposes. Many analytical methods have been proposed for the analysis of amino acids. Until a few decades ago analysis of amino acids via ion-exchange chromatography was by far the most ...
... development of a reliable, rapid and accurate method of analysis for assessing the quality of foods for regulatory purposes. Many analytical methods have been proposed for the analysis of amino acids. Until a few decades ago analysis of amino acids via ion-exchange chromatography was by far the most ...
amino acids
... yields without the bitter β-form byproduct. • may hydrolyze into its constituent amino acids under conditions of elevated temperature or high pH. At room temperature, it is most stable at pH 4.3, where its half-life is nearly 300 days. At pH 7, however, its half-life is only a few days ...
... yields without the bitter β-form byproduct. • may hydrolyze into its constituent amino acids under conditions of elevated temperature or high pH. At room temperature, it is most stable at pH 4.3, where its half-life is nearly 300 days. At pH 7, however, its half-life is only a few days ...
Simultaneous Alignment and Folding of Protein Sequences
... proteins with few known three-dimensional structures. Testing against structurally derived sequence alignments, partiFold-Align significantly outperforms state-of-the-art pairwise sequence alignment tools in the most difficult low sequence homology case and improves secondary structure prediction wh ...
... proteins with few known three-dimensional structures. Testing against structurally derived sequence alignments, partiFold-Align significantly outperforms state-of-the-art pairwise sequence alignment tools in the most difficult low sequence homology case and improves secondary structure prediction wh ...
Changes in chemical composition in male turkeys
... substantially from 3 wk of age onward (Table 1). The composition of FFB at 1 wk of age appeared to be different from the general tendency observed at the other ages. The water content in particular was low (60.3%) at 1 wk of age, resulting in relatively high values for protein, ash, and lipid conten ...
... substantially from 3 wk of age onward (Table 1). The composition of FFB at 1 wk of age appeared to be different from the general tendency observed at the other ages. The water content in particular was low (60.3%) at 1 wk of age, resulting in relatively high values for protein, ash, and lipid conten ...
medbiochem exam, 1999
... You ask about any other incidences of light sensitivity in the family, and learn that this is the first incident observed. Which of the following statements is TRUE? A. The trait may not have been expressed previously because it is recessive. B. The trait was probably not expressed previously becaus ...
... You ask about any other incidences of light sensitivity in the family, and learn that this is the first incident observed. Which of the following statements is TRUE? A. The trait may not have been expressed previously because it is recessive. B. The trait was probably not expressed previously becaus ...
General introduction
... are taken up by the cell and after washing off the excess of radiolabeled compound the release of radioactive transmitters can be initiated. Analysis of the amount of radioactivity in the superfusate and of the amount of radioactivity remaining in the cell enables determination of the percentage of ...
... are taken up by the cell and after washing off the excess of radiolabeled compound the release of radioactive transmitters can be initiated. Analysis of the amount of radioactivity in the superfusate and of the amount of radioactivity remaining in the cell enables determination of the percentage of ...
Tetrahymena Contain Two Distinct and Unusual High Mobility Group
... in large quantities from purified macronuclei along with histone H1 due to their selective solubility in 5 % perchloric acid (Fig. 1 A). Like other HMGs, LG-1 and LG-2 (and H1) can also be extracted from macronuclei with 0.35 M NaCI and are soluble in 2 % TCA (data not shown). The copurification of ...
... in large quantities from purified macronuclei along with histone H1 due to their selective solubility in 5 % perchloric acid (Fig. 1 A). Like other HMGs, LG-1 and LG-2 (and H1) can also be extracted from macronuclei with 0.35 M NaCI and are soluble in 2 % TCA (data not shown). The copurification of ...
Table S1 Genes with similar expression patterns in Qing2
... Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies ...
... Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies ...
Evolution of an ancient protein function involved in
... by the interaction of a transmembrane receptor – which receives local extracellular signals – with Pins, which in turn recruits GKPID, KHC-73, and the spindle microtubules (Yoshiura et al., 2012). In epithelia, in contrast, localization of the complex relative to surrounding cells appears to be medi ...
... by the interaction of a transmembrane receptor – which receives local extracellular signals – with Pins, which in turn recruits GKPID, KHC-73, and the spindle microtubules (Yoshiura et al., 2012). In epithelia, in contrast, localization of the complex relative to surrounding cells appears to be medi ...
Adaptations of protein structure and function to temperature: there is
... Sensitivity to temperature helps determine the success of organisms in all habitats, and is caused by the susceptibility of biochemical processes, including enzyme function, to temperature change. A series of studies using two structurally and catalytically related enzymes, A4-lactate dehydrogenase ...
... Sensitivity to temperature helps determine the success of organisms in all habitats, and is caused by the susceptibility of biochemical processes, including enzyme function, to temperature change. A series of studies using two structurally and catalytically related enzymes, A4-lactate dehydrogenase ...
magamtol talalt cikkek
... regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits. Most of these target PP1c to specific subcellular locations and interact with a small hydrophobic groove on the surface o ...
... regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits. Most of these target PP1c to specific subcellular locations and interact with a small hydrophobic groove on the surface o ...
A Positive Selection Function for miRNA
... The combinations are then translated For example, miRNA let-7 (6) in the 5′ to 3′ has one open reading frame and three open reading frames in its antiparallel complement in the 5′ to 3′ direction. The antiparallel complement would correspond to coding sequences which would appear in mRNA which are ...
... The combinations are then translated For example, miRNA let-7 (6) in the 5′ to 3′ has one open reading frame and three open reading frames in its antiparallel complement in the 5′ to 3′ direction. The antiparallel complement would correspond to coding sequences which would appear in mRNA which are ...
Solution Blowing of Soy Protein Fibers
... to such high temperatures when there must be some thermal degradation of it. Note that PAN shell definitely does not fully disappear under the same thermal treatment but rather carbonizes (12). In addition, it was expected that both materials (soy protein and PAN) shrink differently under such therm ...
... to such high temperatures when there must be some thermal degradation of it. Note that PAN shell definitely does not fully disappear under the same thermal treatment but rather carbonizes (12). In addition, it was expected that both materials (soy protein and PAN) shrink differently under such therm ...
Role of Dietary Soy Protein in Obesity
... 2. Dietary protein and effects on food intake and body weight Ingestion of foods with high protein content is well known to suppress appetite and food intake in humans [14]. Among the three macronutrients (carbohydrate, fat, and protein), protein has the most suppressing effect on food intake. In ad ...
... 2. Dietary protein and effects on food intake and body weight Ingestion of foods with high protein content is well known to suppress appetite and food intake in humans [14]. Among the three macronutrients (carbohydrate, fat, and protein), protein has the most suppressing effect on food intake. In ad ...
1 Proteins: Workshop I Amino Acids
... c. Intermolecular forces are an important aspect of the interaction between a pharmaceutical and its receptor site. Experimental evidence indicates that drugs interact with receptor sites which have protein-like properties. Hydrophobic bonds (London dispersion forces) are formed between non-polar hy ...
... c. Intermolecular forces are an important aspect of the interaction between a pharmaceutical and its receptor site. Experimental evidence indicates that drugs interact with receptor sites which have protein-like properties. Hydrophobic bonds (London dispersion forces) are formed between non-polar hy ...
Nuclear mitotic apparatus protein (NuMA): spindle association
... which each carried an EcoRI recognition site at each end and were complementary to each other, were synthesized. Equal amounts of complementary oligonucleotides were then annealed, followed by EcoRI digestion. The EcoRI-released fragment was then subcloned into the unique EcoRI site of the lacZ gene ...
... which each carried an EcoRI recognition site at each end and were complementary to each other, were synthesized. Equal amounts of complementary oligonucleotides were then annealed, followed by EcoRI digestion. The EcoRI-released fragment was then subcloned into the unique EcoRI site of the lacZ gene ...
ppt - Bio 5068
... NMR Assignments 4D NMR Experiments • Consider a 4D NMR experiment as a collection of 3D NMR experiments still some ambiguities present when correlating multiple 3D triple-resonance experiments 4D NMR experiments make definitive sequential correlations increase in spectral resolution – Overlap ...
... NMR Assignments 4D NMR Experiments • Consider a 4D NMR experiment as a collection of 3D NMR experiments still some ambiguities present when correlating multiple 3D triple-resonance experiments 4D NMR experiments make definitive sequential correlations increase in spectral resolution – Overlap ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.