University of Groningen Expression and engineering of
... In Chapter 3 a sandwiched-culture technique is described, which can be used to screen filamentous fungi for production of heterologous proteins irrespective of proteases in the medium. This technique involves culturing of transformants between perforated polycarbonate membranes and transfer of this ...
... In Chapter 3 a sandwiched-culture technique is described, which can be used to screen filamentous fungi for production of heterologous proteins irrespective of proteases in the medium. This technique involves culturing of transformants between perforated polycarbonate membranes and transfer of this ...
The Anaerobic (Class III) Ribonucleotide Reductase from Lactococcus lactis
... mutants in the L. lactis nrdD gene were still able to grow well under standard anaerobic growth conditions and then overproduced the NrdEF proteins (3). There are three classes of ribonucleotide reductases that differ in their protein structure (see recent reviews in Refs. 2 and 5– 8). All operate v ...
... mutants in the L. lactis nrdD gene were still able to grow well under standard anaerobic growth conditions and then overproduced the NrdEF proteins (3). There are three classes of ribonucleotide reductases that differ in their protein structure (see recent reviews in Refs. 2 and 5– 8). All operate v ...
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... particularly the terminal ones, vary somewhat within a given taxonomic kingdom, all of the SSU active site extensions overlap sequence blocks common to all three taxonomic kingdoms. 6. The 19 Universal LSU Proteins Many of the LSU 19 universal ribosomal proteins share a globular plus nonglobular ext ...
... particularly the terminal ones, vary somewhat within a given taxonomic kingdom, all of the SSU active site extensions overlap sequence blocks common to all three taxonomic kingdoms. 6. The 19 Universal LSU Proteins Many of the LSU 19 universal ribosomal proteins share a globular plus nonglobular ext ...
PROTEOLYSIS is the breakdown of protein to free amino acids
... diagnosis is critical in preventing mental retardation. A diet must be provided that provides adequate, but not excessive, amounts of essential amino acids. Arginine is not normally considered an essential amino acid because it may be synthesized by the action of the urea cycle. Therefore, low-prote ...
... diagnosis is critical in preventing mental retardation. A diet must be provided that provides adequate, but not excessive, amounts of essential amino acids. Arginine is not normally considered an essential amino acid because it may be synthesized by the action of the urea cycle. Therefore, low-prote ...
Regulation of enzyme activity
... Phosphorylation of the enzyme occurs by addition of phosphate group to the enzyme at the hydroxyl group of serine, threonine or tyrosine. This occurs by protein kinase enzyme. Dephosphorylation of the enzyme occurs by removal of phosphate group from the hydroxyl group of serine, threonine or tyrosin ...
... Phosphorylation of the enzyme occurs by addition of phosphate group to the enzyme at the hydroxyl group of serine, threonine or tyrosine. This occurs by protein kinase enzyme. Dephosphorylation of the enzyme occurs by removal of phosphate group from the hydroxyl group of serine, threonine or tyrosin ...
Chapter 3. Enzymes
... a) Binding groups: specifically bind to the substrate to form enzyme-substrate ...
... a) Binding groups: specifically bind to the substrate to form enzyme-substrate ...
Characterisation of the novel proteins expressed in corn line 1507
... ferulic acid and phytic acid, as well as for trypsin inhibitor activity. The levels of these compounds were either well below the limit of detection or, where detectable, were very similar in both the modified corn line 1507 and the non-transformed controls. ...
... ferulic acid and phytic acid, as well as for trypsin inhibitor activity. The levels of these compounds were either well below the limit of detection or, where detectable, were very similar in both the modified corn line 1507 and the non-transformed controls. ...
Enzymes–II
... enzymes behave as ampholytes in an electric field. The isoelectric point (pl) for various enzymes has also been determined. (d) Denaturation. Enzymes, like other proteins, also undergo denaturation. If the crystalline proteinase chymotrypsin is subjected to an unfavourable pH, some part of protein b ...
... enzymes behave as ampholytes in an electric field. The isoelectric point (pl) for various enzymes has also been determined. (d) Denaturation. Enzymes, like other proteins, also undergo denaturation. If the crystalline proteinase chymotrypsin is subjected to an unfavourable pH, some part of protein b ...
Insilico Studies on Taste Receptor Gene (Tas2r38) and Tas2r38
... functions based on the observation that the TAS2R genes are expressed at various extra-oral sites.( W. MEYERHOF)Various TAS2Rs show quite specific agonist profiles, like ptc & prop, have certain chemical substructures in common. In the formation of bitterness receptors, the human body uses “blueprin ...
... functions based on the observation that the TAS2R genes are expressed at various extra-oral sites.( W. MEYERHOF)Various TAS2Rs show quite specific agonist profiles, like ptc & prop, have certain chemical substructures in common. In the formation of bitterness receptors, the human body uses “blueprin ...
Purification, Characterization, and Amino Acid
... death adder, exhibits potent antiplatelet effects. By a combination of gel-filtration, cation-exchange, and reversed-phase chromatographic methods, two inhibitors of platelet aggregation, named acanthin I and II, were purified to homogeneity as assessed by capillary electrophoresis and electrospray ...
... death adder, exhibits potent antiplatelet effects. By a combination of gel-filtration, cation-exchange, and reversed-phase chromatographic methods, two inhibitors of platelet aggregation, named acanthin I and II, were purified to homogeneity as assessed by capillary electrophoresis and electrospray ...
Cloning and Molecular Analysis of the Plasma ... Paramecium tetraurelia
... plasma membrane Ca2+ pump is an enzyme that plays an important role in controlling the concentration of free intracellular Ca2+ in all eukaryotic cells studied thus far. It is the largest of all P-type ATPases [23] with a molecular weight between 128-150 kDa [34]. Plasma membrane Ca2+ pumps constitu ...
... plasma membrane Ca2+ pump is an enzyme that plays an important role in controlling the concentration of free intracellular Ca2+ in all eukaryotic cells studied thus far. It is the largest of all P-type ATPases [23] with a molecular weight between 128-150 kDa [34]. Plasma membrane Ca2+ pumps constitu ...
The Action of Chloramphenicol on Protein and Nucleic Acid
... occurring as part of normal growth, and consequently may have been interdependent. This latter interpretation is clearly supported by the progress curves for the synthesis of nucleic acid. The continued formation of nucleic acid at a logarithmic rate may require the simultaneous continued production ...
... occurring as part of normal growth, and consequently may have been interdependent. This latter interpretation is clearly supported by the progress curves for the synthesis of nucleic acid. The continued formation of nucleic acid at a logarithmic rate may require the simultaneous continued production ...
Comparison of environmental and isolate Sulfobacillus genomes
... using emergent self-organizing maps (ESOM) trained on tetranucleotide frequencies as previously described [9]. Briefly, larger sequences were subdivided into 10 kb fragments, and trained for 100 epochs using the k-batch training method, along with any leftover fragments exceeding 3 kb in length. To ...
... using emergent self-organizing maps (ESOM) trained on tetranucleotide frequencies as previously described [9]. Briefly, larger sequences were subdivided into 10 kb fragments, and trained for 100 epochs using the k-batch training method, along with any leftover fragments exceeding 3 kb in length. To ...
The Action of Chloramphenicol on Protein and Nucleic Acid
... occurring as part of normal growth, and consequently may have been interdependent. This latter interpretation is clearly supported by the progress curves for the synthesis of nucleic acid. The continued formation of nucleic acid at a logarithmic rate may require the simultaneous continued production ...
... occurring as part of normal growth, and consequently may have been interdependent. This latter interpretation is clearly supported by the progress curves for the synthesis of nucleic acid. The continued formation of nucleic acid at a logarithmic rate may require the simultaneous continued production ...
rhizopus oryzae - Journal of Marine Science and Technology
... added and incubated in a boiling water bath for 5 min to develop color [14]. Absorbance at 540 nm was measured to determine chitinolytic activity. One unit (U) of chitinolytic activity was defined as the amount of enzyme that can hydrolyze colloidal chitin and release 1 µg N-acetyl glucosemine equiv ...
... added and incubated in a boiling water bath for 5 min to develop color [14]. Absorbance at 540 nm was measured to determine chitinolytic activity. One unit (U) of chitinolytic activity was defined as the amount of enzyme that can hydrolyze colloidal chitin and release 1 µg N-acetyl glucosemine equiv ...
Integrated Microfluidic Nucleic Acid Isolation, Isothermal
... of target NA in the sample. The chip performs multi tasks to simplify its design, operation, and flow control and to reduce its cost. To summarize, the chip combines the following unit operations in a single reactor: (1) A porous silica or cellulose NA binding “membrane” is used to load sample lysat ...
... of target NA in the sample. The chip performs multi tasks to simplify its design, operation, and flow control and to reduce its cost. To summarize, the chip combines the following unit operations in a single reactor: (1) A porous silica or cellulose NA binding “membrane” is used to load sample lysat ...
Translation
... • Two populations of ribosomes are evident in cells: free ribsomes (in the cytosol) and bound ribosomes (attached to the ER) • Free ribosomes mostly synthesize proteins that function in the cytosol • Bound ribosomes make proteins of the endomembrane system and proteins that are secreted from the cel ...
... • Two populations of ribosomes are evident in cells: free ribsomes (in the cytosol) and bound ribosomes (attached to the ER) • Free ribosomes mostly synthesize proteins that function in the cytosol • Bound ribosomes make proteins of the endomembrane system and proteins that are secreted from the cel ...
Feed Ingredients and Feeds for Channel Catfish
... meal, are more expensive than plant proteins. Although there have been conflicting reports, properly balanced diets with all-plant proteins can provide satisfactory growth of catfish raised in ponds from advanced fingerlings to market size. Soybean meal, dehulled, solvent-extracted, is obtained by ...
... meal, are more expensive than plant proteins. Although there have been conflicting reports, properly balanced diets with all-plant proteins can provide satisfactory growth of catfish raised in ponds from advanced fingerlings to market size. Soybean meal, dehulled, solvent-extracted, is obtained by ...
... showed significant homology. The molecular mass of streptavidin was previously reported to be 60 kDa for the product isolated as described above by an initial ammonium sulfate precipitation (many commercially available products are prepared with such methods). From the gene sequence, a mass of 66 kD ...
2014 Conference Workshop Report
... How the ratio of free drug should be evaluated? It should be evaluated in molar ratio. It seems that Seattle Genetics may be using the rule that up to 2% of the free drug can be present for specific drugs. As the some toxicity may be mediated by the unconjugated payload, reporting the accurate conce ...
... How the ratio of free drug should be evaluated? It should be evaluated in molar ratio. It seems that Seattle Genetics may be using the rule that up to 2% of the free drug can be present for specific drugs. As the some toxicity may be mediated by the unconjugated payload, reporting the accurate conce ...
Functional Characterisation of Plant
... outer membrane proteome between plants and other groups of organisms. Whilst the mitochondrial outer membrane proteome has been studied in model organisms such as Saccharomyces cerevisiae or Neurospora crassa, the components present in Arabidopsis thaliana (Arabidopsis) have been mostly deduced from ...
... outer membrane proteome between plants and other groups of organisms. Whilst the mitochondrial outer membrane proteome has been studied in model organisms such as Saccharomyces cerevisiae or Neurospora crassa, the components present in Arabidopsis thaliana (Arabidopsis) have been mostly deduced from ...
PSI
... [PSI+] is inherited in a non-Mendelian fashion and can be transmitted from one cell to another with cytoplasm No extrachromosomal DNA or RNA have been found to be associated with [PSI+] [PSI+] can be efficiently eliminated by protein denaturing agent, guanidine hydrochloride, or by exposure to ...
... [PSI+] is inherited in a non-Mendelian fashion and can be transmitted from one cell to another with cytoplasm No extrachromosomal DNA or RNA have been found to be associated with [PSI+] [PSI+] can be efficiently eliminated by protein denaturing agent, guanidine hydrochloride, or by exposure to ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.