Click Here to download this tutorial as a PDF
... Interactive version available at http://cbm.msoe.edu/teachingResources/jmol/jmolTraining/structures.html ...
... Interactive version available at http://cbm.msoe.edu/teachingResources/jmol/jmolTraining/structures.html ...
FC Chimeric Fusion Protein Purified Human
... (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissues and cells including the thymus, liver, ovary and lung. FasL is a member of the TNF cytokine family that induces apoptosis by binding to Fas, its cell-surface receptor ...
... (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissues and cells including the thymus, liver, ovary and lung. FasL is a member of the TNF cytokine family that induces apoptosis by binding to Fas, its cell-surface receptor ...
The energy equivalents of ATP and the energy values of food
... It is well established that biochemical energy available to the organism from dietary protein is less than that from an isoenergetic amount of fat or carbohydrate (Krebs, 1964), mainly due to the energy cost of nitrogen excretion as urea (Sallach & Farhren, 1969). Moreover, the energy available, whe ...
... It is well established that biochemical energy available to the organism from dietary protein is less than that from an isoenergetic amount of fat or carbohydrate (Krebs, 1964), mainly due to the energy cost of nitrogen excretion as urea (Sallach & Farhren, 1969). Moreover, the energy available, whe ...
- Wiley Online Library
... An unresolved conundrum in synaptic transmission remains—the observation that SNARE proteins, such as SNAP‑25, are among the most highly expressed, in copy number, presynaptic proteins, whilst only a handful of SNARE complexes are needed to drive the fusion of a single synaptic vesicle [5]. Why, the ...
... An unresolved conundrum in synaptic transmission remains—the observation that SNARE proteins, such as SNAP‑25, are among the most highly expressed, in copy number, presynaptic proteins, whilst only a handful of SNARE complexes are needed to drive the fusion of a single synaptic vesicle [5]. Why, the ...
Molecular mechanisms of platelet exocytosis: insights into the
... patients with HPS and in strains of animals demonstrating HPSlike storage pool defects such as the mocha and pale-ear mice.70 A duplication has been identified in a novel gene (HPS1) in 22 patients of Puerto Rican descent with HPS that was not found in normal controls of Puerto Rican descent or HPS ...
... patients with HPS and in strains of animals demonstrating HPSlike storage pool defects such as the mocha and pale-ear mice.70 A duplication has been identified in a novel gene (HPS1) in 22 patients of Puerto Rican descent with HPS that was not found in normal controls of Puerto Rican descent or HPS ...
exam1ans_2007
... i) the hydrophobic effect is the ordering of water molecules around non-polar groups that become exposed during unfolding (4 pts). It is entropic (+4 pts) and it is the most stabilizing factor for the folded form of the protein (+4 pts). ii) Hydrogen bond involve the donation of a proton from an ele ...
... i) the hydrophobic effect is the ordering of water molecules around non-polar groups that become exposed during unfolding (4 pts). It is entropic (+4 pts) and it is the most stabilizing factor for the folded form of the protein (+4 pts). ii) Hydrogen bond involve the donation of a proton from an ele ...
Sequence, expression, and characterization of the first archaeal ATP
... 50 mM sodium phosphate (pH 6.7 at the respective temperature) and 2 mM F-6-P, 2 mM ATP, and MgCl2:ATP at an optimized ratio of 4:1. Initial velocities were investigated in parallel assays stopped at different time intervals. One unit of ATP-PFK activity is defined as the conversion of 1 µmol F-6-P t ...
... 50 mM sodium phosphate (pH 6.7 at the respective temperature) and 2 mM F-6-P, 2 mM ATP, and MgCl2:ATP at an optimized ratio of 4:1. Initial velocities were investigated in parallel assays stopped at different time intervals. One unit of ATP-PFK activity is defined as the conversion of 1 µmol F-6-P t ...
Self-Subunit Swapping Occurs in Another Gene Type of Cobalt
... the absence of cobalt ion) (final concentration, 0.1 mg/ml) was mixed with the purified cobalt-containing a(StrepP14K)2 [holoa(StrepP14K)2] (final concentration, 0.8 mg/ml) followed by incubation in 10 mM KPB (pH 7.5) at 24uC. As a control, the purified apo-NHase was mixed with cobalt ion (20 mM) wi ...
... the absence of cobalt ion) (final concentration, 0.1 mg/ml) was mixed with the purified cobalt-containing a(StrepP14K)2 [holoa(StrepP14K)2] (final concentration, 0.8 mg/ml) followed by incubation in 10 mM KPB (pH 7.5) at 24uC. As a control, the purified apo-NHase was mixed with cobalt ion (20 mM) wi ...
A Fiber-Optic Electrochemilunescence Sensor Placed into Sample Solution
... The aim of this work is to fabricate a fiber-optic ECL sensor of small size that can be placed into the sample solution and conveniently used in a manner similar to an ion selective electrode. To meet the requirement of practical application, it must have a longer service time and higher repeatabili ...
... The aim of this work is to fabricate a fiber-optic ECL sensor of small size that can be placed into the sample solution and conveniently used in a manner similar to an ion selective electrode. To meet the requirement of practical application, it must have a longer service time and higher repeatabili ...
Supplementary Table 1
... Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance ...
... Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance ...
Functional and structural studies of a C
... peptidyl tRNA in the A site now move to the empty P site. Thus, the ribosome moves along its mRNA in the 3´direction by one codon so that the next aminoacyl tRNA can be delivered to the empty A site. ...
... peptidyl tRNA in the A site now move to the empty P site. Thus, the ribosome moves along its mRNA in the 3´direction by one codon so that the next aminoacyl tRNA can be delivered to the empty A site. ...
and paralogue-specific functions of acyl-CoA
... the nucleus in both human and bovine epithelial cells [14]. ACBD1 or the gene encoding it has been identified in all eukaryotic species examined, and on the basis of both the primary, secondary and tertiary structure it is also highly conserved in eukaryotes [15]. The three-dimensional structure sho ...
... the nucleus in both human and bovine epithelial cells [14]. ACBD1 or the gene encoding it has been identified in all eukaryotic species examined, and on the basis of both the primary, secondary and tertiary structure it is also highly conserved in eukaryotes [15]. The three-dimensional structure sho ...
Chapter 5 - Red Hook Central Schools
... skeleton consisting of four fused rings. • Cholesterol, an important steroid, is a component in animal cell membranes. • Although cholesterol is essential in animals, high levels in the blood may contribute to cardiovascular disease. ...
... skeleton consisting of four fused rings. • Cholesterol, an important steroid, is a component in animal cell membranes. • Although cholesterol is essential in animals, high levels in the blood may contribute to cardiovascular disease. ...
Gene Section BOK (Bcl2-related ovarian killer) Atlas of Genetics and Cytogenetics
... variants. The full-length (Bok-L) mRNA comprises 2.6 kb with the 639 bp open reading frame. The truncated form (Bok-S) results from skipping of exon three and a deletion of 43 bp in the Bok-L coding region. It has been shown that transcription activity of the Bok gene depends on expression of p53 an ...
... variants. The full-length (Bok-L) mRNA comprises 2.6 kb with the 639 bp open reading frame. The truncated form (Bok-S) results from skipping of exon three and a deletion of 43 bp in the Bok-L coding region. It has been shown that transcription activity of the Bok gene depends on expression of p53 an ...
3D Models Enzyme Student Handout
... interaction. This model suggests that enzymes are flexible structures in which the bind of the substrate results in small changes to the shape of the active site, maximizing its interaction with substrate. 10. Describe how the foam catabolism model illustrates the induced fit model of enzyme-substra ...
... interaction. This model suggests that enzymes are flexible structures in which the bind of the substrate results in small changes to the shape of the active site, maximizing its interaction with substrate. 10. Describe how the foam catabolism model illustrates the induced fit model of enzyme-substra ...
IvDimitrov_slides
... epitopes and Allergen-Representative Peptides (ARP) - Iterative pairwise sequence similarity encoding scheme with SVM as the discriminating engine ...
... epitopes and Allergen-Representative Peptides (ARP) - Iterative pairwise sequence similarity encoding scheme with SVM as the discriminating engine ...
Austin Texas Championship Poster
... Motivation and Background In recent years, the ability to expand the genetic code has been made possible by re-coding the amber stop codon, UAG, via the use of modified tRNA synthetase/tRNA pairs. The modified tRNA synthetase charges the modified tRNA with a non-canonical amino acid (ncAA), an amino ...
... Motivation and Background In recent years, the ability to expand the genetic code has been made possible by re-coding the amber stop codon, UAG, via the use of modified tRNA synthetase/tRNA pairs. The modified tRNA synthetase charges the modified tRNA with a non-canonical amino acid (ncAA), an amino ...
View PDF - Faculty Web Sites at the University of Virginia
... current information, because most of the new apparent motors have been linked only in general terms to particular in lliz)o functions. Nevertheless, it is not too early to speculate that the cell makes use of numerous microtubule motors, each of which normally specializes in particular tasks, but ma ...
... current information, because most of the new apparent motors have been linked only in general terms to particular in lliz)o functions. Nevertheless, it is not too early to speculate that the cell makes use of numerous microtubule motors, each of which normally specializes in particular tasks, but ma ...
Hypothesis: Hypoxia induces de novo synthesis of NeuGc
... thereafter washed twice with a solution containing 49 ml phosphate buffered saline (PBS), 1 ml of either fetal bovine serum, human or chicken serum, 200 l EDTA before fixation with 4% paraformaldehyde for 15 minutes. After a third washing step, the cells were incubated in 50 l of primary antibody ...
... thereafter washed twice with a solution containing 49 ml phosphate buffered saline (PBS), 1 ml of either fetal bovine serum, human or chicken serum, 200 l EDTA before fixation with 4% paraformaldehyde for 15 minutes. After a third washing step, the cells were incubated in 50 l of primary antibody ...
Presequence-Independent Manner Transported by the TIM23
... trans compartment, as the electrode of this compartment was directly connected to the headstage of a current amplifier (Axon Gene Clamp 500; Axon Instr.). A self-created analysis software in combination with Origin 6.0 (MicroCal Software Inc.) was used to analyze the current recordings. Miscellaneou ...
... trans compartment, as the electrode of this compartment was directly connected to the headstage of a current amplifier (Axon Gene Clamp 500; Axon Instr.). A self-created analysis software in combination with Origin 6.0 (MicroCal Software Inc.) was used to analyze the current recordings. Miscellaneou ...
Sample and buffer preparation
... Cu2+ ions and reduces them to Cu1+ which shifts color of dye (562 nm) ...
... Cu2+ ions and reduces them to Cu1+ which shifts color of dye (562 nm) ...
Human complement factor H: expression of an additional truncated
... sign of acute phase reaction as assessed by a control hybridization with the SAA probe (Fig. 2). Therefore, the expression of the short factor H mRNA is not linked to an acute phase state. The question arises whether the three distinct factor Hspecific transcripts are encoded by three separate genes ...
... sign of acute phase reaction as assessed by a control hybridization with the SAA probe (Fig. 2). Therefore, the expression of the short factor H mRNA is not linked to an acute phase state. The question arises whether the three distinct factor Hspecific transcripts are encoded by three separate genes ...
nectar composition and membrane transport of sugars and amino
... the maintenance of a sucrose concentration gradient driving passive sucrose diffusion. However, invertases would not be sensitive to the energy transfer inhibitors which block nectar secretion. Conversely, sugar metabolism within the gland tissue could be involved as a driving force osecretion. Suga ...
... the maintenance of a sucrose concentration gradient driving passive sucrose diffusion. However, invertases would not be sensitive to the energy transfer inhibitors which block nectar secretion. Conversely, sugar metabolism within the gland tissue could be involved as a driving force osecretion. Suga ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.