nectar composition and membrane transport of sugars and amino
... the maintenance of a sucrose concentration gradient driving passive sucrose diffusion. However, invertases would not be sensitive to the energy transfer inhibitors which block nectar secretion. Conversely, sugar metabolism within the gland tissue could be involved as a driving force osecretion. Suga ...
... the maintenance of a sucrose concentration gradient driving passive sucrose diffusion. However, invertases would not be sensitive to the energy transfer inhibitors which block nectar secretion. Conversely, sugar metabolism within the gland tissue could be involved as a driving force osecretion. Suga ...
Ch 5 Biomolc Strc & Fxn
... • Steroids- lipids having a carbon skeleton consisting of four fused rings • Cholesterol- important steroid component in animal cell membranes; stabilizes membranes • Cholesterol is essential in animals, but high levels in the blood may contribute to cardiovascular disease ...
... • Steroids- lipids having a carbon skeleton consisting of four fused rings • Cholesterol- important steroid component in animal cell membranes; stabilizes membranes • Cholesterol is essential in animals, but high levels in the blood may contribute to cardiovascular disease ...
Prediction of B cell epitopes
... A data set of 3D discontinuous epitopes A data set of 75 discontinuous epitopes was compiled from structures of antibodies/protein antigen complexes in the PDB The data set has been used for developing a method for predictions of discontinuous B cell epitopes Since about 30 of the PDB entries ...
... A data set of 3D discontinuous epitopes A data set of 75 discontinuous epitopes was compiled from structures of antibodies/protein antigen complexes in the PDB The data set has been used for developing a method for predictions of discontinuous B cell epitopes Since about 30 of the PDB entries ...
Proteome analysis of tobacco BY-2 cell culture - ETH E
... Plastid DNA is organized in a particular structure called “nucleoid” or “plastid nucleus”. These particles are dense, heterogeneous and consist of various DNA-binding proteins, plastid DNA and uncharacterized RNA (Briat et al., 1982). Plastid DNA exists in discrete regions in the form of nucleoids a ...
... Plastid DNA is organized in a particular structure called “nucleoid” or “plastid nucleus”. These particles are dense, heterogeneous and consist of various DNA-binding proteins, plastid DNA and uncharacterized RNA (Briat et al., 1982). Plastid DNA exists in discrete regions in the form of nucleoids a ...
The Structure and Function of Large Biological Molecules
... Steroids are lipids characterized by a carbon skeleton consisting of four fused rings Cholesterol, a type of steroid, is a component in animal cell membranes and a precursor from which other steroids are synthesized Although cholesterol is essential in animals, high levels of cholesterol in th ...
... Steroids are lipids characterized by a carbon skeleton consisting of four fused rings Cholesterol, a type of steroid, is a component in animal cell membranes and a precursor from which other steroids are synthesized Although cholesterol is essential in animals, high levels of cholesterol in th ...
Medicinal Chemistry Reviews
... suppressing tRNA with unnatural amino acids permitted sitespecific incorporation of virtually any amino acid, but it requires a substantial synthetic undertaking and resulted in poor yields of protein from in vitro translation mixtures. In order to achieve this same type of incorporation in living c ...
... suppressing tRNA with unnatural amino acids permitted sitespecific incorporation of virtually any amino acid, but it requires a substantial synthetic undertaking and resulted in poor yields of protein from in vitro translation mixtures. In order to achieve this same type of incorporation in living c ...
... albumin) for 20 min at 20°C, followed by incubation overnight at 4°C with MRK16 (20 µg·mL-1), C219 (10 µg·mL-1), C494 (10 µg·mL-1) or JSB1 (1:10) diluted in blocking serum. Biotinylated secondary antibody (Dako, Glostrup, Denmark) was applied for 30 min, followed by a streptavidin-biotin-conjugated ...
Department of Biochemistry and Molecular - 2016
... to understand the conformational changes proteins undergo and how these changes allow information to flow, both within single proteins and within networks of interacting proteins. Two major application areas are (1) understanding hidden allosteric sites and the opportunities they present for drug de ...
... to understand the conformational changes proteins undergo and how these changes allow information to flow, both within single proteins and within networks of interacting proteins. Two major application areas are (1) understanding hidden allosteric sites and the opportunities they present for drug de ...
Ocimum sanctum Induced hepatic damage R.Bhuvaneswari Dr.K.Jegatheesan
... and animals. Excess of any kind of compounds will be harmful to life [1].Liver plays a major role in detoxification and is generally the major site for intense metabolism[2].It is also a site of biotransformation, of toxic compounds were converted into less harmful form[3].So in this study liver rel ...
... and animals. Excess of any kind of compounds will be harmful to life [1].Liver plays a major role in detoxification and is generally the major site for intense metabolism[2].It is also a site of biotransformation, of toxic compounds were converted into less harmful form[3].So in this study liver rel ...
Document
... the ability of yeast extracts that lacked any living yeast cells to ferment sugar. In a series of experiments at the University of Berlin, he found that the sugar was fermented even when there were no living yeast cells in the mixture. He named the enzyme that brought about the fermentation of sucro ...
... the ability of yeast extracts that lacked any living yeast cells to ferment sugar. In a series of experiments at the University of Berlin, he found that the sugar was fermented even when there were no living yeast cells in the mixture. He named the enzyme that brought about the fermentation of sucro ...
here - Dr. Alan Dombkowski
... Disulfide by Design is an application for the rational design of disulfide bonds in proteins. For a given protein structural model, all residue pairs are rapidly assessed for proximity and geometry consistent with disulfide formation, assuming the residues were mutated to cysteines. The output displ ...
... Disulfide by Design is an application for the rational design of disulfide bonds in proteins. For a given protein structural model, all residue pairs are rapidly assessed for proximity and geometry consistent with disulfide formation, assuming the residues were mutated to cysteines. The output displ ...
Introduction to Carbohydrates
... • The half-life of a protein is influenced by the nature of the N-terminal residue. For example, proteins that have serine as the N-terminal amino acid are long-lived, with a half-life of more than 20 hours. • In contrast, proteins with aspartate as the N-terminal amino acid have a half-life of only ...
... • The half-life of a protein is influenced by the nature of the N-terminal residue. For example, proteins that have serine as the N-terminal amino acid are long-lived, with a half-life of more than 20 hours. • In contrast, proteins with aspartate as the N-terminal amino acid have a half-life of only ...
active site - Blue Valley Schools
... The reactant that an enzyme acts on is called the enzyme’s substrate. The enzyme binds to its substrate, forming an enzyme-substrate complex. The active site is the region on the enzyme where the substrate binds. Induced fit of a substrate brings chemical groups of the active site into positi ...
... The reactant that an enzyme acts on is called the enzyme’s substrate. The enzyme binds to its substrate, forming an enzyme-substrate complex. The active site is the region on the enzyme where the substrate binds. Induced fit of a substrate brings chemical groups of the active site into positi ...
BioN04 Enzymes 2015 v2
... number, the maximum number of substrate molecules converted by one molecule of enzyme per unit time. ...
... number, the maximum number of substrate molecules converted by one molecule of enzyme per unit time. ...
Platelet exocytosis 2000 - Department of Medical Biochemistry
... Although the relationships between the genetic defects of CHS, HPS, and the molecular pathogenesis of abnormal vesicle trafficking and function are not yet fully defined, these insights suggest that normal development of platelet dense granules is related to lysosomal vesicle trafficking. Defects in ...
... Although the relationships between the genetic defects of CHS, HPS, and the molecular pathogenesis of abnormal vesicle trafficking and function are not yet fully defined, these insights suggest that normal development of platelet dense granules is related to lysosomal vesicle trafficking. Defects in ...
Isolation and characterization of (S)
... morphine) and poisons (tubocurarine). The discovery of morphine by the German pharmacist Friedrich W. Sertürner in 1806 began the field of plant alkaloid biochemistry. However, the structure of morphine was not determined until 1952 due to its stereochemical complexity. Major technical advances occu ...
... morphine) and poisons (tubocurarine). The discovery of morphine by the German pharmacist Friedrich W. Sertürner in 1806 began the field of plant alkaloid biochemistry. However, the structure of morphine was not determined until 1952 due to its stereochemical complexity. Major technical advances occu ...
Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
... of Commelina communis using a cell permeabilization method (Brearley and Hanke, 2000). Taken together, these observations support the existence of a functional lipid-independent pathway in higher plants. Ins(1,3,4,5,6)P5 is the common intermediate in both lipid-dependent and lipid-independent phytic ...
... of Commelina communis using a cell permeabilization method (Brearley and Hanke, 2000). Taken together, these observations support the existence of a functional lipid-independent pathway in higher plants. Ins(1,3,4,5,6)P5 is the common intermediate in both lipid-dependent and lipid-independent phytic ...
Refining the Definition of Plant Mitochondrial
... presequences (Lister and Whelan, 2006). Although structural studies reveal the importance of hydrophobic residues for presequence binding, several studies on yeast, mammals, and plants reveal an important role for positively charged residues in presequences for import into mitochondria (Lister et al ...
... presequences (Lister and Whelan, 2006). Although structural studies reveal the importance of hydrophobic residues for presequence binding, several studies on yeast, mammals, and plants reveal an important role for positively charged residues in presequences for import into mitochondria (Lister et al ...
Genome segment 5 of Antheraea mylitta cytoplasmic polyhedrosis
... done in virus infected and virus uninfected mid gut cells of fifth instar A. mylitta larvae using anti-p65 antibody. A major immunoreactive band of approximately 65 kDa was observed in case of infected mid gut cells [Figure 2B (ii), lane 2]; purified polyhedra [Figure 2B (ii), lane 3] and native vir ...
... done in virus infected and virus uninfected mid gut cells of fifth instar A. mylitta larvae using anti-p65 antibody. A major immunoreactive band of approximately 65 kDa was observed in case of infected mid gut cells [Figure 2B (ii), lane 2]; purified polyhedra [Figure 2B (ii), lane 3] and native vir ...
B cell epitopes and predictions
... The problem is analogous to the problems of representing the surface of the earth on a two-dimensional map Reduction of the dimensions leads to distortions of scales, ...
... The problem is analogous to the problems of representing the surface of the earth on a two-dimensional map Reduction of the dimensions leads to distortions of scales, ...
Formation of crystalloid endoplasmic reticulum in
... msALDH and endogenous PDI were exactly colocalized. In the COS-1 cells not expressing msALDH, the immunofluorescence staining of PDI showed a typical reticular pattern (arrow in Fig. 1B). When msALDH was strongly expressed in COS1 cells, large granular structures containing both msALDH and PDI appea ...
... msALDH and endogenous PDI were exactly colocalized. In the COS-1 cells not expressing msALDH, the immunofluorescence staining of PDI showed a typical reticular pattern (arrow in Fig. 1B). When msALDH was strongly expressed in COS1 cells, large granular structures containing both msALDH and PDI appea ...
Protocol Booklet
... (ChIP-PCR), microarrays (ChIP-chip), or sequencing (ChIP-seq). For example, measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while the recruitment of meH3K9 to t ...
... (ChIP-PCR), microarrays (ChIP-chip), or sequencing (ChIP-seq). For example, measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while the recruitment of meH3K9 to t ...
STRUCTURAL INSIGHTS INTO NOVEL MICROBIAL METALLOENZYMES
... Metalloproteins represent a large portion of the total proteome. When bound to a protein a metal ion influences both protein stability and function through structural, catalytic or regulatory roles. Discovery of a metal ion cofactor presents new insight into both the structural and functional aspect ...
... Metalloproteins represent a large portion of the total proteome. When bound to a protein a metal ion influences both protein stability and function through structural, catalytic or regulatory roles. Discovery of a metal ion cofactor presents new insight into both the structural and functional aspect ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.