Instructions for Mem-mEN Web-server
... Membrane proteins, which interact with the membranes of a cell or an organelle, play essential roles in a variety of vital biological processes. Because membrane proteins mediate many interactions between cells and extracellular surroundings as well as between the cytosol and membrane-bound organell ...
... Membrane proteins, which interact with the membranes of a cell or an organelle, play essential roles in a variety of vital biological processes. Because membrane proteins mediate many interactions between cells and extracellular surroundings as well as between the cytosol and membrane-bound organell ...
Plasma membrane Affect shape and function Anchor protein to the
... 1. Impermeable barrier prevent diffusion of water soluble solute 2. Membrane protein mediate transport of specific molecule 3. Maintained by hydrophobic interaction ...
... 1. Impermeable barrier prevent diffusion of water soluble solute 2. Membrane protein mediate transport of specific molecule 3. Maintained by hydrophobic interaction ...
Protein-Surface Interactions
... e.g., immunoglobulins (antibodies)—Y-shaped proteins that bind to antigens (foreign proteins) inducing aggregate formation fibrinogen & thrombin—induce clots by platelet receptor binding • Regulatory: cytokines—regulate cell activities e.g., hormones: insulin (regulates sugar metabolism); growth fac ...
... e.g., immunoglobulins (antibodies)—Y-shaped proteins that bind to antigens (foreign proteins) inducing aggregate formation fibrinogen & thrombin—induce clots by platelet receptor binding • Regulatory: cytokines—regulate cell activities e.g., hormones: insulin (regulates sugar metabolism); growth fac ...
Word Doc - Live Life, Love Fitness
... composed of smaller molecular chains made of carbon, hydrogen, oxygen and nitrogen atoms. There are 50,000 different proteins in the body; everything we are in fact is made of protein, our hair our skin, bone, blood, internal organs, hormones, are all made of protein. Proteins are the primary compon ...
... composed of smaller molecular chains made of carbon, hydrogen, oxygen and nitrogen atoms. There are 50,000 different proteins in the body; everything we are in fact is made of protein, our hair our skin, bone, blood, internal organs, hormones, are all made of protein. Proteins are the primary compon ...
AB123Abstract - PSI AOAPO 2016 Conference
... mechanism of stress-responsive secretion, the suspension-cultured cells of chickpea were subjected to water-deficit conditions. Cell viability of the suspension culture remained unaltered until 96 h, which gradually declined at later stages of dehydration. Proteomic analysis led to the identificatio ...
... mechanism of stress-responsive secretion, the suspension-cultured cells of chickpea were subjected to water-deficit conditions. Cell viability of the suspension culture remained unaltered until 96 h, which gradually declined at later stages of dehydration. Proteomic analysis led to the identificatio ...
Anti-CASK/LIN2 (KA-17) antibody produced in rabbit (C4856
... immunogen a synthetic peptide corresponding to amino acids 393-409 of human CASK/Lin-2 conjugated to keyhole limpet hemocyanin (KLH). This sequence is conserved in mouse and rat. The antibody is affinitypurified using the immunizing peptide immobilized on agarose. Anti-CASK/Lin-2 specifically recogn ...
... immunogen a synthetic peptide corresponding to amino acids 393-409 of human CASK/Lin-2 conjugated to keyhole limpet hemocyanin (KLH). This sequence is conserved in mouse and rat. The antibody is affinitypurified using the immunizing peptide immobilized on agarose. Anti-CASK/Lin-2 specifically recogn ...
(Simple) Physical Models of Protein Folding
... •Linear polymer chain composed of tens (peptides) to thousands (proteins) of monome •Monomers are 20 naturally occurring amino acids •Different proteins have different amino acid sequences •Structureless, extended unfolded state •Compact, ‘unique’ native folded state (with secondary and tertiary str ...
... •Linear polymer chain composed of tens (peptides) to thousands (proteins) of monome •Monomers are 20 naturally occurring amino acids •Different proteins have different amino acid sequences •Structureless, extended unfolded state •Compact, ‘unique’ native folded state (with secondary and tertiary str ...
Protein Complexes – Challenges and Opportunities for
... The traditional gene-based view of drug targets that still governs the industry predicted a maximum of 2000-3000 target genes. Consequently, discovery focuses on a limited set of genes, and compounds are optimized for binding to individual proteins. However, the high attrition rates observed when go ...
... The traditional gene-based view of drug targets that still governs the industry predicted a maximum of 2000-3000 target genes. Consequently, discovery focuses on a limited set of genes, and compounds are optimized for binding to individual proteins. However, the high attrition rates observed when go ...
Chapter 5 Separations: I) Based on Charge or pI A) Electrophoresis
... molecules have the slowest mobility. Can use a UV-VIS detector to detect when proteins are eluting from the column. II) Based on Size or Molecular Weight A) Ultracentrifugation Proteins subjected to a centrifugal force move in the direction of the force at a velocity dependent on mass. Measure the r ...
... molecules have the slowest mobility. Can use a UV-VIS detector to detect when proteins are eluting from the column. II) Based on Size or Molecular Weight A) Ultracentrifugation Proteins subjected to a centrifugal force move in the direction of the force at a velocity dependent on mass. Measure the r ...
Structure of Proteins
... Domains – 3 types: Mostly α-Helix, Mostly β-Sheet, Mixed (α-Helix + β-Sheet) ...
... Domains – 3 types: Mostly α-Helix, Mostly β-Sheet, Mixed (α-Helix + β-Sheet) ...
Dr Asmat Salim MM707-electrophoresis 2014
... types of sensitizers such as thiosulfate ions has been shown to decrease the background and increase detection sensitivity. • After fixing the proteins by methanol/acetic acid the sensitizer is added to the gel which chelates the silver. Silver ions binds to the proteins and can easily and specifica ...
... types of sensitizers such as thiosulfate ions has been shown to decrease the background and increase detection sensitivity. • After fixing the proteins by methanol/acetic acid the sensitizer is added to the gel which chelates the silver. Silver ions binds to the proteins and can easily and specifica ...
Worksheet2_Solutions
... b) One method used to prevent disulfide bond interference with protein sequencing procedures is to reduce disulfide bridges and prevent their reformation with the addition of iodoacetic acid. T c) High molecular weight proteins will migrate farther during gel electrophoresis (SDS-PAGE). F d) -sheet ...
... b) One method used to prevent disulfide bond interference with protein sequencing procedures is to reduce disulfide bridges and prevent their reformation with the addition of iodoacetic acid. T c) High molecular weight proteins will migrate farther during gel electrophoresis (SDS-PAGE). F d) -sheet ...
ECS 189K - UC Davis
... Detecting knots in proteins Think of the main-chain of a protein as being a long entangled string. The question is: does that string form a knot? (i.e. if you were to hold the two extremities of the string and pull, would it result in the formation of a knot, or would the string become linear?) Some ...
... Detecting knots in proteins Think of the main-chain of a protein as being a long entangled string. The question is: does that string form a knot? (i.e. if you were to hold the two extremities of the string and pull, would it result in the formation of a knot, or would the string become linear?) Some ...
Proteins
... Because of their a.a`composition, proteins can bear +ve and –ve charges (amphoteric nature). The pH at which an a.a` or protein has no net charge is known as its isoelectric point. This characteristic is used for separation and quantitation of proteins such as electrophoresis. Solubility: ...
... Because of their a.a`composition, proteins can bear +ve and –ve charges (amphoteric nature). The pH at which an a.a` or protein has no net charge is known as its isoelectric point. This characteristic is used for separation and quantitation of proteins such as electrophoresis. Solubility: ...
Let`s Get Pumped Up about Proteins!!!
... • Typical cell produces ~2000 different proteins • 9 different categories depending on function (Table 2.1 pg. 57) ...
... • Typical cell produces ~2000 different proteins • 9 different categories depending on function (Table 2.1 pg. 57) ...
• - Cambridge Isotope Laboratories
... human membrane proteins or receptors. M-fold has developed methods for expressing G protein-coupled receptors (GPCRs) in bacteria utilizing stable isotope labeled media and refolding proteins into biologically active forms. GPCRs are involved in a wide range of biological activities (blood pressure, ...
... human membrane proteins or receptors. M-fold has developed methods for expressing G protein-coupled receptors (GPCRs) in bacteria utilizing stable isotope labeled media and refolding proteins into biologically active forms. GPCRs are involved in a wide range of biological activities (blood pressure, ...
The basis of specific ligand recognition by proteins
... high-resolution structures of complexes between proteins and drugs, DNA or simply other proteins [1]. However, what really determines binding specificity is still largely unknown, as the very limited success of drug design and protein modelling clearly shows. Many observations on proteins demonstrat ...
... high-resolution structures of complexes between proteins and drugs, DNA or simply other proteins [1]. However, what really determines binding specificity is still largely unknown, as the very limited success of drug design and protein modelling clearly shows. Many observations on proteins demonstrat ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.