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... carbohydrates the night before a game? ...
Complete protein with pulses, Chickpea Tortilla or Vegan
Complete protein with pulses, Chickpea Tortilla or Vegan

... Vegetables, your choice! [1/2 onion (peeled and roughly chopped), 1 clove garlic (peeled and crushed), corn (1/3 cup), 1/2 red pepper (cored and chopped), potato (partly cooked and chopped), sweet potato (partly cooked and chopped), ...
Organic Chemistry
Organic Chemistry

... • 4 Other types of biologically important Lipids – Phospholipids - Important for membrane structure – Steroids - eg. Cholesterol & testosterone. Provide membrane support / serve as hormones – Terpenes - serve as important components of pigments – Prostaglandins - appear to act like localized hormone ...
Organic Chemistry - Biology Junction
Organic Chemistry - Biology Junction

... • 4 Other types of biologically important Lipids – Phospholipids - Important for membrane structure – Steroids - eg. Cholesterol & testosterone. Provide membrane support / serve as hormones – Terpenes - serve as important components of pigments – Prostaglandins - appear to act like localized hormone ...
Organic Chemistry - Welcome to Cherokee High School
Organic Chemistry - Welcome to Cherokee High School

... • 4 Other types of biologically important Lipids – Phospholipids - Important for membrane structure – Steroids - eg. Cholesterol & testosterone. Provide membrane support / serve as hormones – Terpenes - serve as important components of pigments – Prostaglandins - appear to act like localized hormone ...
Protein Folding 2 Lattice Model
Protein Folding 2 Lattice Model

... Evolving a foldable sequence = sequence with a large energy gap Three approaches to obtain foldable sequences: En ! E Ec ! E ...
03 - summer worksheet
03 - summer worksheet

... Five-carbon sugar occurring in RNA Sugar present in milk; formed from glucose and galactose Main structural material in some external skeletons and other hard body parts of some animals and fungi Animal starch, stored especially in liver and muscle tissue; formed from glucose chains ...
The Two Major Membrane Skeletal Proteins (Articulins) of Euglena
The Two Major Membrane Skeletal Proteins (Articulins) of Euglena

... (Rosiere et al., 1990). Actin is not present in the membrane skeleton, and the microtubules found in vivo (Leedale, 1964; Mignot, 1965; Dubreuil and Bouck, 1985) are not required for in vitro assembly of the membrane skeleton. To determine the relationship of the articulins to one another and to oth ...
Problem 1
Problem 1

... (a) Indicate the order in which these proteins will elute from a gel-filtration column (starting with the one that elutes first). You can use letters A-E (see table) for simplicity. C, A, B, E, D (b) You load this mixture on a cation exchange column (i.e. column that bears negatively charged groups) ...
Proteomics Center University of Missouri
Proteomics Center University of Missouri

... – detergent – breaks open cell compartments – reducing agent – breaks specific protein bonds – heat – break peptide bonds to “linearize” protein ...
4th seminar (ELISA, functional)_2016
4th seminar (ELISA, functional)_2016

... ELISA systems can be used not only for the detection of a material, but its exact concentration can also be measured by the help of a known concentration standard ...
The Exocytic/Lysosomal Transport Pathway
The Exocytic/Lysosomal Transport Pathway

... Directed Translocation of Secretory Vesicles • apical targeting in caveolin coated, lipidraft vesicles • basolateral targeting signals – direct protein to this membrane ...
Introduction to Virology
Introduction to Virology

... infant mortality in the developing world. Viruses spread between hosts by direct transmission, contaminated food or water, or arthropod vectors. ...
Food Chemistry for 1..
Food Chemistry for 1..

... apart if the temperature is higher • Peptide bonds can also be broken apart by acid ...
Biomolecules
Biomolecules

PROTEIN SYNTHESIS STEP 1:​ ​Transcription
PROTEIN SYNTHESIS STEP 1:​ ​Transcription

... If the protein needs to have a specific sequence of amino acids,  then how does the tRNA know which amino acid to bring to the  ribosome?  ● There are only 20 different amino acids.   ● There are four different bases (A, U, C, G).   ● Each codon is made of 3 bases (letters).  ● That means there are  ...
Structural Genomics - University of Houston
Structural Genomics - University of Houston

... protein. If each residue is considered to have just 3 possible conformations the total number of conformations of the protein is 3100. Conformational changes occur on a time scale of 10-13 seconds i.e. the time required to sample all possible conformations would be 3100 x 10-13 seconds which is abou ...
Supplementary Figures
Supplementary Figures

... boiled in SDS-PAGE sample buffer for 3 minutes and sonicated to reduce viscosity of released phage DNA. Samples were electrophoresed through 10% acrylamide gels and protein bands visualized by Coomassie staining. Individual bands were excised and trypsinized. The peptide mixture of these digested pr ...
Protein regulation: The statistical theory of
Protein regulation: The statistical theory of

... made through the use of NMR spectroscopy, which provides a variety of tools for characterizing the structure and dynamics of proteins3–6. Particularly attractive in this context is the use of chemical shifts, because these parameters can be measured with great accuracy and under a wide variety of di ...
Slide 1
Slide 1

... Detail of interactions between recognition helix and DNA ...
In vivo chloroplast protein synthesis by the
In vivo chloroplast protein synthesis by the

... IgG was purified from the antisera by passage over a CM Affi-Gel Blue column (Bio-Rad) followed by precipitationwith 45% (NH4)2S04. The precipitate was desalted by passage over Sephadex G-50 and concentrated in an Amicon ultrafiltration unit. Antigens were detected essentially according to Towbin et ...
Supporting Information Legends Supplementary Table S1
Supporting Information Legends Supplementary Table S1

... Supplementary Figure S6. Hypothetical model illustrating SlSUT2-brassinosteroid interaction. The model illustrates a potential sucrose efflux into the periarbuscular space from where it is retrieved by SlSUT2 back into the plant root cells. Efflux might potentially be mediated by still uncharacteriz ...
Protein Synthesis
Protein Synthesis

... (instructions) for making a specific protein • The average gene is 3000 bases • Total number of genes is 30,000 to ...
See advert for details
See advert for details

... includes 12 European laboratories and companies which cooperatively develop research on photosynthetic energy conversion into biomass and biofuels. Salary is competitive and include benefits. The successful candidate: - is within 5 years from her/his master degree. - is strongly motivated to carry o ...
Protein foods - Deans Community High School
Protein foods - Deans Community High School

...  the iodine test turns from brown to black if starch is in food.  the filter paper test goes transparent if fat is in food. Chemists also have a way to test whether foods contain protein. This test is called the soda lime test. Watch your teacher demonstrate (demo 3.40) what happens in the soda li ...
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Western blot



The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.
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