Cellular Defence Mechanisms
... Immunity – ability to resist infectious disease Non-specific e.g. phagocytosis - provides a general protection Specific - specialised response against an infectious agent E.g. antibody production ...
... Immunity – ability to resist infectious disease Non-specific e.g. phagocytosis - provides a general protection Specific - specialised response against an infectious agent E.g. antibody production ...
Grooving Down the Helix
... National Laboratory, Harvard University, and the Indian Institute of Science has made a major step in understanding how molecules locate the genetic information in DNA that is necessary to carry out important biological processes. The research, published in the December 1, 2009 edition of Nature Str ...
... National Laboratory, Harvard University, and the Indian Institute of Science has made a major step in understanding how molecules locate the genetic information in DNA that is necessary to carry out important biological processes. The research, published in the December 1, 2009 edition of Nature Str ...
Protein Folding Problem
... Peptide chains can be cross-linked by disulfides, Zinc, heme or other liganding compounds. Zinc has a complete d orbital , one stable oxidation state and forms ligands with sulfur, nitrogen and oxygen. Proteins refold very rapidly and generally in only one stable conformation. ...
... Peptide chains can be cross-linked by disulfides, Zinc, heme or other liganding compounds. Zinc has a complete d orbital , one stable oxidation state and forms ligands with sulfur, nitrogen and oxygen. Proteins refold very rapidly and generally in only one stable conformation. ...
Chapter Five: The Structure and Function of Macromolecules
... 4. Think about the structure of fatty acids. What do they all have in common? ...
... 4. Think about the structure of fatty acids. What do they all have in common? ...
Lecture exam 1A
... E. None of the above is correct 3. Which of the following statements, if any, is FALSE? If none of A-D are false select E. A. Protein folding often occurs spontaneously B. Chaperones are specific proteins involved in protein folding C. The final tertiary structure of a protein is dictated by its ami ...
... E. None of the above is correct 3. Which of the following statements, if any, is FALSE? If none of A-D are false select E. A. Protein folding often occurs spontaneously B. Chaperones are specific proteins involved in protein folding C. The final tertiary structure of a protein is dictated by its ami ...
Post-translational Modification Learning Objective Post
... 2. Protein translation: The process by which the mRNA template is read by ribosomes to synthesize the corresponding protein molecule on the basis of the three letter codons, which code for specific amino acids. 3. Cytosol: A cellular compartment that serves as the site for protein synthesis. 4. Sign ...
... 2. Protein translation: The process by which the mRNA template is read by ribosomes to synthesize the corresponding protein molecule on the basis of the three letter codons, which code for specific amino acids. 3. Cytosol: A cellular compartment that serves as the site for protein synthesis. 4. Sign ...
PAGE n MALDI for reference - AOS-HCI-2011-Research
... Mentored by: Ms. Sandra Tan Co-Mentored by: Mr. Goh Yan Yih ...
... Mentored by: Ms. Sandra Tan Co-Mentored by: Mr. Goh Yan Yih ...
Homo sapiens glucokinase (hexokinase 4) (GCK), transcript variant 1
... Summary: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localiz ...
... Summary: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localiz ...
Biochemistry Presentation Notes Pre-AP 14-15
... *Enzymes can create or break down molecules at incredible rates!!!* ...
... *Enzymes can create or break down molecules at incredible rates!!!* ...
Ribosomes and The Golgi Apparatus
... Over view: Golgi Body The golgi apparatus adds carbohydrates and lipids to ...
... Over view: Golgi Body The golgi apparatus adds carbohydrates and lipids to ...
Methods S1.
... chondrocytes were electrophoresed and separated on a 4-20% Tris-HCl gel (BioRad, Hercules, CA) and transferred to a Hybond-ECL nitrocellulose membrane (Amersham Biosciences, Piscataway, NJ). The membrane was probed with antibodies described in suppl. Table 3. The nitrocellulose membranes were then e ...
... chondrocytes were electrophoresed and separated on a 4-20% Tris-HCl gel (BioRad, Hercules, CA) and transferred to a Hybond-ECL nitrocellulose membrane (Amersham Biosciences, Piscataway, NJ). The membrane was probed with antibodies described in suppl. Table 3. The nitrocellulose membranes were then e ...
Slide 1
... • Some irregular viruses do exist and many of these have underlying helical or icosohedral symmetry. • Note-viruses form regular shapes but use irregular proteins to do so. • This creates a problem that must be solved for assembly to occur. • For example, it would be easy to imagine how a virus migh ...
... • Some irregular viruses do exist and many of these have underlying helical or icosohedral symmetry. • Note-viruses form regular shapes but use irregular proteins to do so. • This creates a problem that must be solved for assembly to occur. • For example, it would be easy to imagine how a virus migh ...
Proteins Animal By-Products Seed By
... The bodies of our livestock, pets, and ourselves have many uses for proteins. Enzymes, antibodies, many hormones, and substances that help the blood to clot, are all proteins. Proteins form part of muscles and many cell structures, including the cell membrane. During the process of digestion, protei ...
... The bodies of our livestock, pets, and ourselves have many uses for proteins. Enzymes, antibodies, many hormones, and substances that help the blood to clot, are all proteins. Proteins form part of muscles and many cell structures, including the cell membrane. During the process of digestion, protei ...
BIOCHEMISTRY (CHEM 360)
... explain your answer for 10.2 (why each messenger goes where it does). IP3 is water soluble and is capable of reaching the organelles in the cytosol DAG is hydrophobic and stays in the lipophilic regions in the plasma membrane ...
... explain your answer for 10.2 (why each messenger goes where it does). IP3 is water soluble and is capable of reaching the organelles in the cytosol DAG is hydrophobic and stays in the lipophilic regions in the plasma membrane ...
Gene7-08
... released into the cytosol after synthesis on free ribosomes. Some have signals for targeting to organelles such as the nucleus or mitochondria. Proteins that are localized cotranslationally associate with the ER membrane during synthesis, so their ribosomes are "membranebound". The proteins pass int ...
... released into the cytosol after synthesis on free ribosomes. Some have signals for targeting to organelles such as the nucleus or mitochondria. Proteins that are localized cotranslationally associate with the ER membrane during synthesis, so their ribosomes are "membranebound". The proteins pass int ...
Biochemical studies on antioxidant
... activities of the aqueous extracts of the above plants towards protein and estimation of some markers of the protein oxidation were also investigated. Methods: The antioxidant activities of the above plants extracts, such as superoxide dismutase (SOD)- like and scavenging of diphenyl picrylhydrazyl ...
... activities of the aqueous extracts of the above plants towards protein and estimation of some markers of the protein oxidation were also investigated. Methods: The antioxidant activities of the above plants extracts, such as superoxide dismutase (SOD)- like and scavenging of diphenyl picrylhydrazyl ...
Chapter 5 Lecture Notes: Microbial Nutrition
... 3. Passage of compounds through outer membrane occurs via specific and "nonspecific" porins via simple diffusion (therefore, the concentration in the periplasm must be less than that of the external environment – see simple diffusion below) 4. Passage of compounds through sieve-like periplasm withou ...
... 3. Passage of compounds through outer membrane occurs via specific and "nonspecific" porins via simple diffusion (therefore, the concentration in the periplasm must be less than that of the external environment – see simple diffusion below) 4. Passage of compounds through sieve-like periplasm withou ...
Detection of protein-protein interactions
... • Expression of target and bait protein fusions are induced by IPTG. • If bait and target interact, RNApol initiates transcription of His3 and Strepr genes. • Screening 1st for His prototrophy, 2nd for streptomycin resistance. ...
... • Expression of target and bait protein fusions are induced by IPTG. • If bait and target interact, RNApol initiates transcription of His3 and Strepr genes. • Screening 1st for His prototrophy, 2nd for streptomycin resistance. ...
Chapter 6 Section 3
... A large, complex polymer composed of carbon, hydrogen, oxygen, nitrogen and sometimes sulfur Essential to all life/provides structure for tissues and organs Amino Acids—basic building blocks of all proteins Proteins are bonded by PEPTIDE BONDS ...
... A large, complex polymer composed of carbon, hydrogen, oxygen, nitrogen and sometimes sulfur Essential to all life/provides structure for tissues and organs Amino Acids—basic building blocks of all proteins Proteins are bonded by PEPTIDE BONDS ...
Notes_Biochemistry_Short_Course
... Importance: These criteria are used to minimize damage to the healthy tissue from the radiation. The purpose of the radiation is to damage the faster growing cancer cells or to highlight the fast working cells but cause no additional damage to other cells. ...
... Importance: These criteria are used to minimize damage to the healthy tissue from the radiation. The purpose of the radiation is to damage the faster growing cancer cells or to highlight the fast working cells but cause no additional damage to other cells. ...
Sample Preparation Methods for MS Based Proteomics
... 2. Wash with 25mM ABC in 70% ACN 3. Reduce with 10mM DTT in 25mM ABC Alkylate with 50mM IAA in 25mM ABC 4. Wash with 25mM ABC in 70% CAN (dehydrate) 5. Digest with trypsin in 25mM ABC over night 6. Extract peptides with 5% FA in 50% ACN 7. Remove ACN before LCMSMS analysis ...
... 2. Wash with 25mM ABC in 70% ACN 3. Reduce with 10mM DTT in 25mM ABC Alkylate with 50mM IAA in 25mM ABC 4. Wash with 25mM ABC in 70% CAN (dehydrate) 5. Digest with trypsin in 25mM ABC over night 6. Extract peptides with 5% FA in 50% ACN 7. Remove ACN before LCMSMS analysis ...
Document
... A COOH (carboxy) end that “loses” a H+ ion A NH2 (amino) end that “takes” a H+ ion More than 170 known, but only 20 are coded by nucleic acids and “used” to make proteins 19 are l-chiral (left-handed) & one is symmetric Carboxy & amino ends “plug” together to form a peptide bond and thus make long c ...
... A COOH (carboxy) end that “loses” a H+ ion A NH2 (amino) end that “takes” a H+ ion More than 170 known, but only 20 are coded by nucleic acids and “used” to make proteins 19 are l-chiral (left-handed) & one is symmetric Carboxy & amino ends “plug” together to form a peptide bond and thus make long c ...
Chemistry part 2
... which it can function • Each enzyme has an optimal pH in which it can function • Tertiary structure can be radically altered by changes in pH ...
... which it can function • Each enzyme has an optimal pH in which it can function • Tertiary structure can be radically altered by changes in pH ...
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[Business Communication]
... Dogma: central and peripheral • Most genes in higher organisms contain internal untranslated regions, or introns. • Some regions of DNA sequence are devoted to control mechanism • Substantial amount of the genomes appears to be “junk” (we do not yet understand) • DNA molecules are chemically simila ...
... Dogma: central and peripheral • Most genes in higher organisms contain internal untranslated regions, or introns. • Some regions of DNA sequence are devoted to control mechanism • Substantial amount of the genomes appears to be “junk” (we do not yet understand) • DNA molecules are chemically simila ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.