Development of an HTRF Antibody Screening Assay for Cell Line
... In the production of monoclonal antibodies there is increasing demand to generate high producing cell lines and this usually requires screening large number of transfectants. Selecting the appropriate screening assay is important as the throughput of the assay is critical and a potential bottle neck ...
... In the production of monoclonal antibodies there is increasing demand to generate high producing cell lines and this usually requires screening large number of transfectants. Selecting the appropriate screening assay is important as the throughput of the assay is critical and a potential bottle neck ...
1 Lecture 6: Protein Primary, Secondary and Tertiary Structure +
... Native Structure: The native conformation of a given protein is its functionally active conformation. This is a well-defined arrangement of atoms in three-dimensional space that generates a complex surface topography. The catalytic, binding, and structural roles played by proteins are all dependent ...
... Native Structure: The native conformation of a given protein is its functionally active conformation. This is a well-defined arrangement of atoms in three-dimensional space that generates a complex surface topography. The catalytic, binding, and structural roles played by proteins are all dependent ...
A Glossary of Terms Used in the Biotherapeutic Industry
... Fragment antigen binding site. Antibodies are Y-shaped molecules. The arms of each Y are the FAb regions that bind to antigens; the stem of the Y is the Fc region, which attracts microbe-engulfing cells to destroy what has been bound. If the active part of an antibody can be identified, sometimes on ...
... Fragment antigen binding site. Antibodies are Y-shaped molecules. The arms of each Y are the FAb regions that bind to antigens; the stem of the Y is the Fc region, which attracts microbe-engulfing cells to destroy what has been bound. If the active part of an antibody can be identified, sometimes on ...
ppt
... VAST – Automated classification http://www.ncbi.nlm.nih.gov/Structure/VAST/vasthelp.html All against all BLAST comparison of NCBI’s MMDB (database of known protein structure at NCBI, derived from the PDB) Clustered into groups by a neighbor joining procedure, using BLAST p-value cutoffs of C or les ...
... VAST – Automated classification http://www.ncbi.nlm.nih.gov/Structure/VAST/vasthelp.html All against all BLAST comparison of NCBI’s MMDB (database of known protein structure at NCBI, derived from the PDB) Clustered into groups by a neighbor joining procedure, using BLAST p-value cutoffs of C or les ...
Document
... • Tertiary structure – Interaction among the R groups of amino acids – Interactions include ionic bonds, hydrogen bonds, hydrophobic interactions, and covalent bonds – What type of amino acids involved in each? ...
... • Tertiary structure – Interaction among the R groups of amino acids – Interactions include ionic bonds, hydrogen bonds, hydrophobic interactions, and covalent bonds – What type of amino acids involved in each? ...
doc - University of California, Santa Cruz
... about the biological and evolutionary significance of introns. We therefore need a simple way of investigating those, and the enzymes involved in the intron turnover pathway. The target enzyme of the study, the RNA lariat debranching enzyme (DBR) from mosquito-borne parasitic protozoan Plasmodium fa ...
... about the biological and evolutionary significance of introns. We therefore need a simple way of investigating those, and the enzymes involved in the intron turnover pathway. The target enzyme of the study, the RNA lariat debranching enzyme (DBR) from mosquito-borne parasitic protozoan Plasmodium fa ...
Ch 5 Activity List File
... 9. Identify an ester linkage and describe how it is formed. 10. Distinguish between saturated and unsaturated fats. 11. Name the principal energy storage molecules of plants and animals. 12. Distinguish between a protein and a polypeptide. 13. Explain how a peptide bond forms between two amino acids ...
... 9. Identify an ester linkage and describe how it is formed. 10. Distinguish between saturated and unsaturated fats. 11. Name the principal energy storage molecules of plants and animals. 12. Distinguish between a protein and a polypeptide. 13. Explain how a peptide bond forms between two amino acids ...
A Toc75-like protein import channel is abundant in chloroplasts
... antisera might be infected by bacteria and thus developed antibodies to polypeptides of the pathogen. In order to characterize the 66 kDa protein further, the purified outer envelope membranes were either treated with the protease thermolysin to determine whether the polypeptide contains surface exp ...
... antisera might be infected by bacteria and thus developed antibodies to polypeptides of the pathogen. In order to characterize the 66 kDa protein further, the purified outer envelope membranes were either treated with the protease thermolysin to determine whether the polypeptide contains surface exp ...
Psoriasis
... • Fusion proteins are fully human constructs between the constant portion of an immunoglobulin molecule and the binding site of a receptor ( designated “cept”) ...
... • Fusion proteins are fully human constructs between the constant portion of an immunoglobulin molecule and the binding site of a receptor ( designated “cept”) ...
A Toc75like protein import channel is abundant in
... antisera might be infected by bacteria and thus developed antibodies to polypeptides of the pathogen. In order to characterize the 66 kDa protein further, the purified outer envelope membranes were either treated with the protease thermolysin to determine whether the polypeptide contains surface exp ...
... antisera might be infected by bacteria and thus developed antibodies to polypeptides of the pathogen. In order to characterize the 66 kDa protein further, the purified outer envelope membranes were either treated with the protease thermolysin to determine whether the polypeptide contains surface exp ...
Functional genomics: assigning functions to genome sequences
... Combining Inferences of CoEvolution from 4 Methods We use a Bayesian approach to combine the probabilities from the four methods to arrive at a single probability that two proteins co-evolve: ...
... Combining Inferences of CoEvolution from 4 Methods We use a Bayesian approach to combine the probabilities from the four methods to arrive at a single probability that two proteins co-evolve: ...
Some Like it Cool
... The details of hydration still raise fundamental questions relevant to a large variety of problems in chemistry and biology. We have shown the THz spectroscopy in combination with MD simulations is a powerful took to study the sub-nsec hydration. THz spectroscopy is also able to reveal the important ...
... The details of hydration still raise fundamental questions relevant to a large variety of problems in chemistry and biology. We have shown the THz spectroscopy in combination with MD simulations is a powerful took to study the sub-nsec hydration. THz spectroscopy is also able to reveal the important ...
Full Text - BioTechniques
... negative control, staining was also performed on guinea pig tumors induced by a guinea pig tumor cell line (Line 10). To confirm identity of blood vessels, sections were also stained with anti-VEGF:VEGFR antibody GV39 or anti-CD31 antibody 9G11. Proteins directed against the VEGF:VEGFR complex were ...
... negative control, staining was also performed on guinea pig tumors induced by a guinea pig tumor cell line (Line 10). To confirm identity of blood vessels, sections were also stained with anti-VEGF:VEGFR antibody GV39 or anti-CD31 antibody 9G11. Proteins directed against the VEGF:VEGFR complex were ...
Journal of Bacteriology
... malate dehydrogenase activity. A comparison of the proteins of the various cell fractions by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (Fig. 2, lanes C, D, E, and F) confirms that the four cell fractions have unique protein patterns. In conclusion, the two methods described here enabl ...
... malate dehydrogenase activity. A comparison of the proteins of the various cell fractions by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (Fig. 2, lanes C, D, E, and F) confirms that the four cell fractions have unique protein patterns. In conclusion, the two methods described here enabl ...
On the Importance of Amino Acid Sequence and Spatial Proximity of
... analysis is done is the range of interaction distances studied, which is mostly irrelevant to protein structure. The authors measure the cumulative distribution of the number of Cα-Cα interactions over the range 0–60 Å for specific residue types and fit this to simple sigmoids. The fits show no clea ...
... analysis is done is the range of interaction distances studied, which is mostly irrelevant to protein structure. The authors measure the cumulative distribution of the number of Cα-Cα interactions over the range 0–60 Å for specific residue types and fit this to simple sigmoids. The fits show no clea ...
Cardiomyopathy: myozap for the heart Immunity: FOXO maintains
... Cancer often arises from a combination of multiple genetic changes, each conferring characteristics that predispose cells to cause disease. T-cell acute lymphoblastic leukemia (T-ALL) can result from mutations leading to the overexpression of oncogenic transcription factors, such as LMO2, during cel ...
... Cancer often arises from a combination of multiple genetic changes, each conferring characteristics that predispose cells to cause disease. T-cell acute lymphoblastic leukemia (T-ALL) can result from mutations leading to the overexpression of oncogenic transcription factors, such as LMO2, during cel ...
How do we purify proteins? GFP as model system to learn
... sonication, Microbial cells pressure, or tissue psmotic ...
... sonication, Microbial cells pressure, or tissue psmotic ...
A simple and efficient method for the purification
... Kawaguchi.H. el al. (1989) Nucleic Acids Res. 17, 6229-6240. Ronai.Z. and Weinstein.I.B. (1990) Cancer Res. 50, 5374-5381. DignamJ.D. et al. (1983) Nucleic Acids Res. 11, 1475-1489. ...
... Kawaguchi.H. el al. (1989) Nucleic Acids Res. 17, 6229-6240. Ronai.Z. and Weinstein.I.B. (1990) Cancer Res. 50, 5374-5381. DignamJ.D. et al. (1983) Nucleic Acids Res. 11, 1475-1489. ...
Study Guide for Understanding the Concept of Protein Synthesis
... Individual "escort" ribosomes from the cytoplasm appear to the amino acids. At the presence of these ribosomes, the amino acids release. Step #4: Transfer RNA (tRNA) acts as a "taxi" by which the "escort" ribosomes take the amino acids and position them into place as Ribosomal RNA (rRNA). Step #5: R ...
... Individual "escort" ribosomes from the cytoplasm appear to the amino acids. At the presence of these ribosomes, the amino acids release. Step #4: Transfer RNA (tRNA) acts as a "taxi" by which the "escort" ribosomes take the amino acids and position them into place as Ribosomal RNA (rRNA). Step #5: R ...
Slides #5B (Green)
... MS for identifying proteins in a mixture Protein interactions Important types of proteins ...
... MS for identifying proteins in a mixture Protein interactions Important types of proteins ...
The SPFH domain - Tavernarakis Lab
... Caenorhabditis elegans encodes nine stomatin-related genes, three of which have been genetically characterized. ...
... Caenorhabditis elegans encodes nine stomatin-related genes, three of which have been genetically characterized. ...
Bonding is more than attraction
... - It is a long chain of smaller molecules called nucleotides. • What is a nucleotide? - A nucleotide has three parts: a sugar, a base, and a phosphate group, which contains phosphorus and oxygen atoms. ...
... - It is a long chain of smaller molecules called nucleotides. • What is a nucleotide? - A nucleotide has three parts: a sugar, a base, and a phosphate group, which contains phosphorus and oxygen atoms. ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.