DIFFUSION, OSMOSIS AND CELLULAR TRANSPORT
... specific for one chemical; binding of substrate causes shape change in transport protein ...
... specific for one chemical; binding of substrate causes shape change in transport protein ...
Protein and its functional properties in food
... Protein is made up of small units called amino acids. Amino acids are compounds which contain carbon, hydrogen, oxygen and nitrogen. A few also contain sulphur and phosphorous. There are around 20 different amino acids commonly found in plant and animal proteins. All amino acids have an acid group ( ...
... Protein is made up of small units called amino acids. Amino acids are compounds which contain carbon, hydrogen, oxygen and nitrogen. A few also contain sulphur and phosphorous. There are around 20 different amino acids commonly found in plant and animal proteins. All amino acids have an acid group ( ...
Nutrients - Food a fact of life
... Protein is made up of small units called amino acids. Amino acids are compounds which contain carbon, hydrogen, oxygen and nitrogen. A few also contain sulphur and phosphorous. There are around 20 different amino acids commonly found in plant and animal proteins. All amino acids have an acid group ( ...
... Protein is made up of small units called amino acids. Amino acids are compounds which contain carbon, hydrogen, oxygen and nitrogen. A few also contain sulphur and phosphorous. There are around 20 different amino acids commonly found in plant and animal proteins. All amino acids have an acid group ( ...
Anti-Actin, a-Smooth Muscle antibody, Mouse monoclonal, clone
... same population of smooth muscle cells during development, pathological situations and different culture conditions. The actin in cells of various species and tissue origin are very similar in their immunological and physical properties. ...
... same population of smooth muscle cells during development, pathological situations and different culture conditions. The actin in cells of various species and tissue origin are very similar in their immunological and physical properties. ...
Organic Molecules Study Guide: Substance Basic Structure
... Which organic molecule is known for insulation? _______lipids__________ Which organic molecule is found in cellular membranes? ________lipids________ Which organic molecule helps with protein synthesis and carries genetic information? __Nucleic ...
... Which organic molecule is known for insulation? _______lipids__________ Which organic molecule is found in cellular membranes? ________lipids________ Which organic molecule helps with protein synthesis and carries genetic information? __Nucleic ...
workshops: absences: examinations: textbook
... explain their nomenclature; to explain how their different properties place them into different environments in proteins. 20 'common' amino acids used in proteins. The CORN Law denotes L isomer amino acid. 6 key features of an amino acid: Size Hydrophobic Character Shape Hydrogen bonding capacity Ch ...
... explain their nomenclature; to explain how their different properties place them into different environments in proteins. 20 'common' amino acids used in proteins. The CORN Law denotes L isomer amino acid. 6 key features of an amino acid: Size Hydrophobic Character Shape Hydrogen bonding capacity Ch ...
Diffusion _ Cell Transport Powerpoint
... CARBON DIOXIDE IS HIGH, BECAUSE IT IS GENERATED INSIDE THE CELL BY RESPIRATION. THE CONCENTRATION OF CO2 IS MUCH LOWER OUTSIDE THE CELL. THE CELL MEMBRANE IS FREELY PERMEABLE TO CO2 AND THEREFORE IT DIFFUSES INTO THE EXTRACELLULAR FLUID- DOWN ITS CONCENTRATION GRADIENT-AND THEN INTO BLOODSTREAM. ...
... CARBON DIOXIDE IS HIGH, BECAUSE IT IS GENERATED INSIDE THE CELL BY RESPIRATION. THE CONCENTRATION OF CO2 IS MUCH LOWER OUTSIDE THE CELL. THE CELL MEMBRANE IS FREELY PERMEABLE TO CO2 AND THEREFORE IT DIFFUSES INTO THE EXTRACELLULAR FLUID- DOWN ITS CONCENTRATION GRADIENT-AND THEN INTO BLOODSTREAM. ...
Red meat and protein
... Diets must provide the right balance of amino acids and nitrogen essential for the body to be able to synthesise protein for growth and maintenance. Protein quality is a measure of how well or poorly the body can use a given protein to meet its needs. This is dependent on the essential amino acid co ...
... Diets must provide the right balance of amino acids and nitrogen essential for the body to be able to synthesise protein for growth and maintenance. Protein quality is a measure of how well or poorly the body can use a given protein to meet its needs. This is dependent on the essential amino acid co ...
April 3 lecture slides
... Structures like this involving DNA with bound activator proteins and RNA polymerase complex are names “enhanceosomes”. TBP stands for TATAbinding protein, a component of RNA polymerase II associated factor, TFIID ...
... Structures like this involving DNA with bound activator proteins and RNA polymerase complex are names “enhanceosomes”. TBP stands for TATAbinding protein, a component of RNA polymerase II associated factor, TFIID ...
Test Correction Rubric
... Proteins are composed of any combination of 20 amino acids joined together by peptide bonds. The sequence of the amino acids is the primary order of the protein structure and ultimately determines the ...
... Proteins are composed of any combination of 20 amino acids joined together by peptide bonds. The sequence of the amino acids is the primary order of the protein structure and ultimately determines the ...
Lecture2-2010
... Both His12 and His119 are protonated in the enzymeinhibitor complex. The proton is protected from exchange by the presence of the inhibitor. Need to go to higher pH to remove it. ...
... Both His12 and His119 are protonated in the enzymeinhibitor complex. The proton is protected from exchange by the presence of the inhibitor. Need to go to higher pH to remove it. ...
Carbon Compounds
... • Examples - most animal fats ( butter, lard, grease, etc.) • These are the “Unhealthy” ones! ...
... • Examples - most animal fats ( butter, lard, grease, etc.) • These are the “Unhealthy” ones! ...
The Science of Proteins in Milk (including A1 vs A2 Milk)
... Claims suggested that A1 milk caused autism, ischaemic ...
... Claims suggested that A1 milk caused autism, ischaemic ...
Lectrure 9 - Columbus Labs
... • The complex amino acid mixture in the hydrolysate obtained after digestion of a protein in 6 N HCl can be separated into the component amino acids by either ion exchange chromatography (separation by charge) or reverse-phase chromatography (separation by polarity) • Both of these methods of separa ...
... • The complex amino acid mixture in the hydrolysate obtained after digestion of a protein in 6 N HCl can be separated into the component amino acids by either ion exchange chromatography (separation by charge) or reverse-phase chromatography (separation by polarity) • Both of these methods of separa ...
lecture 3
... proteins in isolated (pure) systems may not behave as they do in the cell - binding partner(s) might be missing - post-translational modifications might be missing ...
... proteins in isolated (pure) systems may not behave as they do in the cell - binding partner(s) might be missing - post-translational modifications might be missing ...
Gene Section ATF1 (activating transcription factor 1) Atlas of Genetics and Cytogenetics
... The chimaeric protein is composed of the N-terminal domain of EWS linked to the bZIP domain of ATF-1. Oncogenesis Binds to ATF sites present in cAMP-responsive promoters via the ATF1 bZIP domain and activates transcription constitutively, dependent on the activation domain (EAD) present in EWSR1. ...
... The chimaeric protein is composed of the N-terminal domain of EWS linked to the bZIP domain of ATF-1. Oncogenesis Binds to ATF sites present in cAMP-responsive promoters via the ATF1 bZIP domain and activates transcription constitutively, dependent on the activation domain (EAD) present in EWSR1. ...
Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe
... • incubate 30min at room temperature in Blocking Solution, rocking optional • incubate 30min at room temperature with 30ml Blocking Solution + 2µl anti-DIG-AP Conjugate (premix before adding to blot), rocking optional • wash 2X 15min at room temperature with 100ml Washing Buffer on rocker • equilibr ...
... • incubate 30min at room temperature in Blocking Solution, rocking optional • incubate 30min at room temperature with 30ml Blocking Solution + 2µl anti-DIG-AP Conjugate (premix before adding to blot), rocking optional • wash 2X 15min at room temperature with 100ml Washing Buffer on rocker • equilibr ...
Lecture 2 - cell assembly
... • Cell wall structure • Two distinct groups of bacteria with very different cell walls – Gram negative has an outer lipid membrane (different from the inner, or plasma membrane) – Gram positive lacks the outer membrane but has a ...
... • Cell wall structure • Two distinct groups of bacteria with very different cell walls – Gram negative has an outer lipid membrane (different from the inner, or plasma membrane) – Gram positive lacks the outer membrane but has a ...
Gene Ontology (GO)
... A motif (or fingerprint) is a short, conserved region of a protein. Its size is often 10 to 20 amino acids. Simple motifs include transmembrane domains and phosphorylation sites. These do not imply homology when found in a group of proteins. In PROSITE,a pattern is a qualitative motif description (a ...
... A motif (or fingerprint) is a short, conserved region of a protein. Its size is often 10 to 20 amino acids. Simple motifs include transmembrane domains and phosphorylation sites. These do not imply homology when found in a group of proteins. In PROSITE,a pattern is a qualitative motif description (a ...
Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe
... • wash 2X 15min at room temperature with 2X SSC, 0.1% SDS on rocker • wash 2X 15min at 42°C with 0.5X SSC, 0.1% SDS on rocker (washes can be modified to control stringency – this is fairly stringent) • rinse in Washing Buffer • incubate 30min at room temperature in Blocking Solution, rocking optiona ...
... • wash 2X 15min at room temperature with 2X SSC, 0.1% SDS on rocker • wash 2X 15min at 42°C with 0.5X SSC, 0.1% SDS on rocker (washes can be modified to control stringency – this is fairly stringent) • rinse in Washing Buffer • incubate 30min at room temperature in Blocking Solution, rocking optiona ...
Chem*3560 Lecture 19: Review of regulation
... Liver enzyme contains a protein kinase A target sequence at Ser 32; when phophorylated, it masks the PFK2 site and exposes the FBPase 2 site. Cyclic AMP and Protein kinase A promote glycogen breakdown and gluconeogenesis in liver, so that glucose can be released into the blood. Muscle enzyme contain ...
... Liver enzyme contains a protein kinase A target sequence at Ser 32; when phophorylated, it masks the PFK2 site and exposes the FBPase 2 site. Cyclic AMP and Protein kinase A promote glycogen breakdown and gluconeogenesis in liver, so that glucose can be released into the blood. Muscle enzyme contain ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.